Article
Microbiology
Xiafei Zheng, Zhongneng Yan, Chenxi Zhao, Lin He, Zhihua Lin, Minhai Liu
Summary: This study investigated the diversity, dynamics, drivers, and assembly mechanisms of total bacteria, napA, and nosZ denitrifiers in intensive shrimp aquaculture ponds. The results showed that the diversity of the total bacterial community increased over time, while the diversity of napA and nosZ bacteria remained stable. The community structure changed significantly over the culture period and multiple factors were identified as drivers of the denitrifying bacterial communities. Network analysis revealed complex co-occurrence patterns between total, napA, and nosZ bacteria, which fluctuated over time. The null model approach showed that napA and nosZ bacteria were primarily governed by deterministic processes. The findings provide important ecological insights for optimizing microbial community structure and function.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yue Hou, Duoying Zhang, Haoran Cao, Yanlong Zhang, Dandan Zhao, Weimin Zeng, Hong Lei, Ye Bai
Summary: In this study, a strain called Psychrobacter cryohalolentis F5-6 was isolated and found to have a high aerobic-denitrifying rate at low temperatures. It has a high percentage of unsaturated fatty acids and short chain saturated acids. The strain prefers acetate as the carbon source for denitrification. The study also showed the nitrate metabolism processes of the strain and determined the optimal conditions for denitrification. This research provides valuable information and a potential bacterial resource for improving nitrate removal from low C/N water at low temperatures.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Engineering, Environmental
Shizong Wang, Jun Hu, Jianlong Wang
Summary: A stable denitrifying microbial consortium capable of degrading triclosan was isolated from a denitrifying bioreactor. The denitrification efficiency was higher under anoxic condition. Triclosan had a negative impact on denitrification under both aerobic and anoxic conditions.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
Article
Engineering, Environmental
Maria Jose De la Fuente, Rodrigo De la Iglesia, Laura Farias, Holger Daims, Michael Lukumbuzya, Ignacio T. Vargas
Summary: This study successfully enriched marine denitrifying microorganisms by applying a specific potential, -260 mV (vs. Ag/AgCl), demonstrating significantly higher nitrate removal compared to other conditions. The polarized electrodes were found to work as external electron donors for nitrate reduction, indicating the feasibility of enriching denitrifying microorganisms without the need for a culture medium, exogenous electron donor, or previously enriched inoculum.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Environmental Sciences
Fabio Manca, Carla Wegscheidl, Rhianna Robinson, Suzette Argent, Christopher Algar, Daniele De Rosa, Matthew Griffiths, Fiona George, David Rowlings, Louis Schipper, Peter Grace
Summary: This study trialed different designs of woodchip bioreactors to quantify their NO3- removal performance in sugar cane farms in North Queensland. The results showed that the bioreactors were effective at reducing NO3- runoff to the Great Barrier Reef, with the beds exhibiting a higher NO3- removal rate compared to the wall.
Article
Engineering, Environmental
Meng Jiang, Yating Wu, Shiyin Hu, Qingxi Li, Shouwen Chen
Summary: This study enriched a high-efficiency denitrification community by gradually shifting the COD/N ratio and assembled a simplified denitrification microbial consortium. The novel consortium achieved a 98.4% total nitrogen removal rate at a COD/N ratio of 3.2 and enhanced the denitrification performance of nitrate-contaminated groundwater under low COD/N ratios.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Soil Science
Xiya Wang, Yujiao Li, Ignacio A. Ciampitti, Ping He, Xinpeng Xu, Shaojun Qiu, Shicheng Zhao
Summary: The results of this study indicate that the rate of straw return significantly affects soil denitrification potential and the community composition of denitrifying bacteria, with increasing straw return rates leading to higher soil DP and abundance of nirK and nirS genes. However, the a-diversity of the denitrifying communities remained unaffected by chemical fertilization and straw rates.
APPLIED SOIL ECOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiaotian Zuo, Wei Xu, Shiping Wei, Shuangcheng Jiang, Yu Luo, Minghuang Ling, Kai Zhang, Yuanhao Gao, Zhichao Wang, Jiege Hu, Hans-Peter Grossart, Zhuhua Luo
Summary: In recent years, the use of mixed microbial cultures for nitrogen removal has gained attention due to cooperative metabolism. A natural bacterial-fungal consortium isolated from mariculture showed excellent aerobic denitrification capacity, with nitrate removal and denitrification efficiencies reaching 100% and 44.27%, respectively. High-throughput sequencing and network analysis revealed that the co-occurrence of bacterial genera such as Vibrio and fungal genera such as Fusarium potentially drove the aerobic denitrification process. The consortium also exhibited high steady aerobic denitrification performance in sub-culturing experiments, highlighting its potential for biotechnology applications.
Article
Agricultural Engineering
Lei He, Xuejie He, Xing Fan, Shuohui Shi, Tao Yang, Hongyuan Li, Jian Zhou
Summary: This study found that the use of S. oneidensis MR-1 in combination with denitrifying microbial community can significantly enhance the bio-denitrification process through interspecies electron transfer (IET). The nitrogen removal rate was increased by 74.74% and the accumulation of nitrite was greatly reduced. The results also indicated that mediated interspecies electron transfer (MIET) played a role in enhancing denitrification, as observed when S. oneidensis MR-1 was separated by a dialysis bag.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Chen Chen, Pingping Liu, Yingjie Liu, Yuquan Wei, Ji Li, Guo-chun Ding
Summary: This study investigated the changes in the abundance and diversity of denitrifying and DNRA bacterial communities in anaerobic subsoil in response to carbon amendment. The results showed that straw amendment significantly reduced nitrate leaching and increased total nitrogen content in the subsoil. It also enriched specific bacterial communities and influenced the reassembly of microbial communities.
JOURNAL OF SOILS AND SEDIMENTS
(2023)
Article
Biotechnology & Applied Microbiology
Chen Chen, Hui Han, Ya Meng, Haiqing Gong, Rui Jia, Ting Xu, Guo-Chun Ding, Ji Li
Summary: The study found that carbon amendment in greenhouse soils can significantly reduce nitrate leaching, increase nitrogen content in the subsoil, and enrich specific bacterial genera. Carbon amendment may achieve these effects by activating denitrifying and anaerobic bacterial populations.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Review
Engineering, Multidisciplinary
Yuchuan Fan, Jie Zhuang, Michael Essington, Sindhu Jagadamma, John Schwartz, Jaehoon Lee
Summary: This study summarizes 41 types of substrates using a dataset collected from 63 peer-reviewed articles, which include 219 independent denitrifying bioreactor units. The analysis indicates that woodchips and corncobs are the most cost-effective substrates among natural carbon substrates, and multiple materials substrates are recommended as the optimal choice, especially woodchip-based and corncob-based substrates, which have great potential for improvement. This analysis can assist in optimizing the design of denitrifying bioreactors to meet the environmental, economic, and practical requirements of users.
Article
Biochemistry & Molecular Biology
Hui Zhang, Lin Song, Xiaolin Chen, Pengcheng Li
Summary: The study showed that using seaweed polysaccharides as stimulant can promote the growth and enhance the nitrate removal efficiency of denitrifying bacteria, which is significant in mitigating soil salinization, improving nitrogen utilization efficiency, and avoiding toxic effects on plants and the environment.
Article
Environmental Sciences
Shuaimin Chen, Eiko E. Kuramae, Zhongjun Jia, Binbin Liu
Summary: This study utilized stable isotope probing and molecular biology techniques to investigate the composition and function of active denitrifying microorganisms in the vadose zone. It revealed significant differences in the composition and function of active denitrifiers between different soil layers. This research improves our understanding of active denitrifying microbes in the vadose zone and provides insights for reducing nitrate pollution in groundwater.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Environmental Sciences
Yinfeng Xia, Debao Lu, Yiting Qi, Han Chen, Yufeng Zhao, Yu Bai, Lifang Zhu, Nan Geng, Cundong Xu, Ertian Hua
Summary: A biochar electrode based biofilm reactor was developed for advanced removal of nitrate from agricultural runoff. By optimizing operation conditions and utilizing bio-electrochemical reduction, high nitrate removal efficiency could be achieved. The biochar electrode material significantly promotes the utilization efficiency of cathode electrons by microorganisms, thereby improving the denitrification capacity.