Article
Biotechnology & Applied Microbiology
L. H. L. Hermans, M. A. J. Van Kelle, P. J. A. Oomen, R. G. P. Lopata, S. Loerakker, C. V. C. Bouten
Summary: This study investigates the influence of anisotropic and isotropic mechanical loading on tissue-engineered cardiovascular constructs. The results show that the (an)isotropic loading induced by the initial scaffold geometry differentially affects the mechanical state, geometry, and final structural organization of the constructs over time.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Pilar Montero-Calle, Maria Flandes-Iparraguirre, Konstantinos Mountris, Ana de la Nava, Nicolas Laita, Ricardo M. Rosales, Olalla Iglesias-Garcia, Elena M. de-Juan-Pardo, Felipe Atienza, Maria Eugenia Fernandez-Santos, Estefania Pena, Manuel Doblare, Juan J. Gavira, Francisco Fernandez-Aviles, Felipe Prosper, Esther Pueyo, Manuel M. Mazo
Summary: Biofabrication of human tissues has experienced significant growth, but challenges remain in generating cardiac tissue with adequate mechanical properties and predictable evolution. In this study, the researchers used MEW and hiPSC-derived cardiac cells to create fibre-reinforced human cardiac minitissues. The minitissues displayed advanced maturation, improved cellular alignment, and enhanced functional performance compared to conventional 2D controls. The researchers also developed computational models and simulations to predict the post-fabrication evolution of the cardiac minitissues.
Article
Engineering, Biomedical
Zhiyan Hao, Sen Wang, Jichang Nie, Dichen Li, Ao Fang, Jianfeng Kang, Chaozong Liu, Ling Wang
Summary: The translation of tissue-engineered cartilage (TEC) into potential alternatives for articular cartilage defect repair has been found to vary from natural cartilage properties. Mechanical stimulation, such as compressive load, has been shown to influence matrix remodeling in TEC. However, the effects of shear from tissue fluid phase remain understudied. Research suggests that larger strains and higher frequencies are favorable for cell proliferation and extracellular matrix synthesis in TEC, and the combined mechanical stimulation is more beneficial for matrix remodeling than single loading motion.
BIO-DESIGN AND MANUFACTURING
(2021)
Article
Biochemistry & Molecular Biology
Akbar Teguh Prakoso, Hasan Basri, Dendy Adanta, Irsyadi Yani, Muhammad Imam Ammarullah, Imam Akbar, Farah Amira Ghazali, Ardiyansyah Syahrom, Tunku Kamarul
Summary: Permeability is an important factor to consider in the design of porous scaffolds for cell migration and bone tissue regeneration. This study developed a porous scaffold based on the morphological indices of cancellous bone and analyzed fluid flow through the scaffold using computational fluid dynamics. The permeability of the scaffold was compared to that of natural cancellous bone and different scaffold types. The results showed that the Negative Schwarz Primitive (NSP) scaffold had similar permeability to natural cancellous bone and could be manipulated by changing the curvature to support cell migration and tissue regeneration.
Article
Cell & Tissue Engineering
Cecillia Lui, Alexander F. Chin, Seungman Park, Enoch Yeung, Chulan Kwon, Gordon Tomaselli, Yun Chen, Narutoshi Hibino
Summary: The study aimed to create scaffold-free, 3D-printed cardiac tissue grafts from hiPSC-derived CMs and assess the impact of mechanical stimulation on graft maturation. Results showed that mechanical stretching enhanced tissue development, increasing sarcomere length, improving extracellular matrix alignment, and upregulating cardiac-specific gene expression. This highlights the importance of mechanical conditioning for engineering mature scaffold-free cardiac tissue grafts.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Chemistry, Physical
Tiago H. Pires, John W. C. Dunlop, Andre P. G. Castro, Paulo R. Fernandes
Summary: When designing bone tissue engineering scaffolds, the wall shear stress (WSS) generated by fluid flow is an important factor to consider. This study used computational fluid dynamics (CFD) analysis to evaluate the average WSS in different scaffold topologies and found that a smooth surface topology with tetrahedral elements resulted in 35% higher WSS compared to a non-smooth surface topology with hexahedral elements. The study also demonstrated the efficacy of using the optimization algorithm simulated annealing to design scaffolds with specific average WSS levels.
Article
Multidisciplinary Sciences
M. I. Rizzo, L. Tomao, S. Tedesco, M. Cajozzo, M. Esposito, C. De Stefanis, A. M. Ferranti, D. Mezzogori, A. Palmieri, G. Pozzato, M. Algeri, F. Locatelli, L. Leone, M. Zama
Summary: This study developed a novel natural scaffold derived from a decellularized porcine mucoperiosteum, engineered by an innovative micro-perforation procedure and recellularized with human bone marrow-derived mesenchymal stem cells. The new scaffold showed efficiency in supporting hMSC engraftment, spreading, and differentiation, as well as increased potential for enhanced palatal bone regeneration. This innovative tissue engineering protocol could have significant implications for future clinical applications in human cleft lip and palate repair.
SCIENTIFIC REPORTS
(2021)
Review
Engineering, Biomedical
Leanne de Silva, Paulina N. Bernal, A. J. W. Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Summary: The development of tissue engineering strategies for treatment of large bone defects is crucial, and creating a vascular network is vital for tissue engineered bone constructs.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Multidisciplinary
Fangwei Qi, Xiuwen Gao, Yang Shuai, Shuping Peng, Youwen Deng, Sheng Yang, Youwen Yang, Cijun Shuai
Summary: A wireless electrical stimulation system based on magnetoelectric coupling effect was constructed, which effectively promoted cell proliferation and gene expression, and provided new possibilities for bone repair.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Engineering, Biomedical
Elisa Fiume, Alessandro Schiavi, Gissur Orlygsson, Cristina Bignardi, Enrica Verne, Francesco Baino
Summary: Proper microstructural and transport properties are crucial for scaffold design in tissue engineering applications. Evaluating permeability remains a challenge, while pore characteristics are relatively easier to assess.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Multidisciplinary
Changsong Zhou, Shenghui Su, Jiwen Fan, Jiuzao Lin, Xiaoyong Wang
Summary: In this study, PLGA/Si3N4 composite nanofiber scaffolds were successfully prepared and showed promising potential for bone regeneration due to their enhanced mechanical strength and biological activity.
FRONTIERS IN MATERIALS
(2022)
Article
Multidisciplinary Sciences
Lan Li, Peng Wang, Huixin Liang, Jing Jin, Yibo Zhang, Jianping Shi, Yun Zhang, Siyuan He, Hongli Mao, Bin Xue, Jiancheng Lai, Liya Zhu, Qing Jiang
Summary: The Haversian system-like gradient structure can effectively promote bone regeneration. This structural design, based on triply periodic minimal surface architectures, with pore size varying from the edge to the center, can be used as a new solution for the clinical application of prosthesis design.
JOURNAL OF ADVANCED RESEARCH
(2023)
Review
Polymer Science
Mohammad Javad Javid-Naderi, Javad Behravan, Negar Karimi-Hajishohreh, Shirin Toosi
Summary: Damage or loss of bone tissue is a major issue affecting human well-being, and tissue engineering is used to recover and regenerate bone fractures and damage. Polymer biomaterials are the most commonly required materials for synthetic bone scaffolds due to their suitable mechanical properties and degradation rates. Synthetic polymers offer advantages in bone replacement because they can be designed and changed easily, and their biocompatibility has been widely accepted in tissue engineering. This article updates on materials and application usage of scaffolds in bone tissue engineering for hard tissue regeneration.
POLYMERS FOR ADVANCED TECHNOLOGIES
(2023)
Article
Engineering, Chemical
Muhammad Azfar Noordin, Mohamad Ikhwan Kori, Abdul Hadi Abdul Wahab, Ardiyansyah Syahrom, Amir Putra Md Saad
Summary: The dynamic characteristic of bone allows it to remodel itself through mechanobiological responses. This study investigates the fluid flow properties when bone scaffold is integrated into cancellous bone at different skeletal sites. The results show significant differences in the localization and maximum value of shear stress between independent bone scaffold and bone scaffold integrated with cancellous bone.
TRANSPORT IN POROUS MEDIA
(2022)
Review
Chemistry, Multidisciplinary
Teng Wan, Meng Zhang, Hao-Ran Jiang, Yi-Chong Zhang, Xiao-Meng Zhang, Yi-Lin Wang, Pei-Xun Zhang
Summary: This review provides an overview of the current state-of-the-art research in developing and using nanomaterials for better bone injury repair. It discusses the hierarchical architecture of natural bone as the basis for constructing bone substitutes and the potential of nanomaterials to overcome urgent clinical issues associated with bone injury. The review also describes the characteristics and results of related bone engineering studies on inorganic and organic nanomaterials, inspiring therapeutic strategies with potential for clinical applications.
Article
Engineering, Biomedical
Katarzyna Polak-Krasna, Ali Reza Abaei, Reyhaneh Neghabat Shirazi, Eoin Parle, Oliver Carroll, William Ronan, Ted J. Vaughan
Summary: This study systematically evaluated the physical, thermal, and mechanical performance of medical-grade semi-crystalline PLLA undergoing thermally-accelerated degradation. The results showed a reduction in molecular weight and melting temperature, an increase in percent crystallinity, and an increase in brittleness and degradation rate over time. Despite maintaining elastic modulus and yield strength, a drastic reduction in tensile failure strain was observed beyond day 49, indicating a substantial deterioration in load-bearing capacity.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Engineering, Biomedical
Ciara G. McKenna, Ted J. Vaughan
Summary: A computational framework was developed in this study to predict the functional performance of self-expanding wire braided stents, with braid angle identified as a key governing parameter for stent performance. The addition of a polymeric cover was found to cause a stiffer radial response for the wire braided stents.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Materials Science, Biomaterials
Felix Benn, Nadja Kroger, Max Zinser, Kerstin van Gaalen, Ted J. Vaughan, Ming Yan, Ralf Smeets, Eric Bibiza, Savko Malinov, Fraser Buchanan, Alexander Kopp
Summary: The further development of future Magnesium based biodegradable implants needs to consider both design freedom and implant volume reduction, with laser powder bed fusion (LPBF) being introduced as a promising manufacturing method.
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2021)
Article
Multidisciplinary Sciences
Ivor P. Geoghegan, Laoise M. McNamara, David A. Hoey
Summary: Estrogen deficiency in post-menopausal osteoporosis leads to osteoclastogenesis and bone loss. Increased pro-osteoclastogenic signaling by osteocytes following estrogen withdrawal is associated with a disrupted actin cytoskeleton. Hedgehog signaling in osteocytes is involved in RANKL production, and the elongation of cilia is linked to a reduction in focal adhesions and actin contractility.
SCIENTIFIC REPORTS
(2021)
Review
Chemistry, Multidisciplinary
Gabriele Nasello, Mar Condor, Ted Vaughan, Jessica Schiavi
Summary: The recent development of bone-on-chips (BOCs) utilizes minimal cell and material quantity and incorporates hydrogels to create a three-dimensional culture environment. This technology allows for mimicking various cell growth environments and personalized therapy development.
APPLIED SCIENCES-BASEL
(2021)
Article
Physiology
Lin Qi, Wenbo Zhu, Wei Qian, Lisheng Xu, Ying He, Feihu Zhao
Summary: The study evaluated the performance of a catheter with a spherical-tip design in post-dilation, showing that the spherical-tip catheter is easier and safer to pass through a stent in a curved vessel. The finite element analysis and experimental validation demonstrated the consistency of the design, making it a useful tool for future optimization and evaluation of catheters.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Feihu Zhao, Yi Xiong, Keita Ito, Bert van Rietbergen, Sandra Hofmann
Summary: Mechanobiology research aims to understand the role of mechanics in cell physiology and pathology, especially in the study of bone physiology and pathology. Researchers can influence the cell response by adjusting scaffold pore geometries and the micro-mechanical environment in bioreactors. Future work may involve using artificial intelligence-assisted techniques for automatic design of solid porous scaffold geometry to optimize the micro-mechanical environment.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biophysics
Mahdi Tavakol, Ted J. Vaughan
Summary: This study investigated the role of calcium ions on energy dissipation at the interface between osteopontin (OPN) and hydroxyapatite (HAp). It found that calcium ions greatly influenced the structure and energy dissipation performance of OPN proteins. Under small deformation, the compact OPN structure facilitated greater energy dissipation through mechanisms mediated by surface-bound calcium and sacrificial bond breaking. At larger deformation, the compact structure also enabled OPN to dissipate higher energy. Phosphorylation of OPN also played an important role in energy dissipation.
BIOPHYSICAL JOURNAL
(2022)
Article
Engineering, Biomedical
Constantino Fiuza, Katarzyna Polak-Krasna, Luca Antonini, Lorenza Petrini, Oliver Carroll, William Ronan, Ted J. Vaughan
Summary: The study evaluated the mechanical, micro-mechanical, and physical properties of Reva Medical Fantom Encore Bioresorbable Scaffolds subjected to thermally-accelerated degradation. Results showed an initial increase in radial strength and stiffness, followed by a decrease in ductility and load-bearing capacity with continued degradation. The scaffolds exhibited increased crystallinity throughout the degradation process, with minimal changes in nano-mechanical properties.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Review
Endocrinology & Metabolism
Laoise M. McNamara
Summary: Postmenopausal osteoporosis is not only characterized by bone loss due to decreased estrogen levels, but also by alterations in mechanosensitive osteocytes. Research shows that estrogen deficiency affects the osteocyte mechanical and microenvironment, leading to impaired mechanobiological responses and exacerbating osteocyte regulation of osteoclasts. Understanding these changes is crucial for developing targeted therapies to inhibit resorption and secondary mineralization in osteoporosis.
CURRENT OSTEOPOROSIS REPORTS
(2021)
Article
Cardiac & Cardiovascular Systems
Ciara G. McKenna, Ted J. Vaughan
Summary: This study found that stent covering increases initial axial stiffness and up to 60% greater radial resistive force at small crimp diameters. The use of a stiffer PET polymeric covering material significantly alters the radial and axial response of the stent.
CARDIOVASCULAR ENGINEERING AND TECHNOLOGY
(2022)
Article
Engineering, Biomedical
Kerstin van Gaalen, Felix Gremse, Felix Benn, Peter E. McHugh, Alexander Kopp, Ted J. Vaughan
Summary: This study develops an automated detection framework (PitScan) using Python-based algorithm to evaluate the severity and phenomenology of pitting corrosion. The framework analyzes microcomputer-tomography scans (mu CT) of corroded cylindrical specimens and identifies surface-based corrosion features, providing a spatial characterization of pitting parameters. The study also establishes relationships between key pitting parameters and mechanical performance in Rare Earth Magnesium alloy specimens. The results show that certain parameters described in ASTM G46-94 have little correlation with mechanical performance, while parameters directly linked to the reduction of cross-sectional area are more indicative of specimen's mechanical performance. The developed automated detection framework has the potential to standardize pitting corrosion measurements and predict mechanical strength degradation over time.
BIOACTIVE MATERIALS
(2022)
Article
Biology
Monika Colombo, Anna Corti, Diego Gallo, Andrea Colombo, Giacomo Antognoli, Martina Bernini, Ciara McKenna, Scott Berceli, Ted Vaughan, Francesco Migliavacca, Claudio Chiastra
Summary: This study analyzed the impact of stent design and stent overlapping on patient-specific SFA hemodynamics. The results showed that different stent designs and overlapping configurations significantly altered the hemodynamic parameters. Stent design type and overlapping length had a significant impact on the hemodynamics.
COMPUTERS IN BIOLOGY AND MEDICINE
(2022)
Article
Engineering, Biomedical
Hamid Alijani, Ted J. Vaughan
Summary: This study investigates the effects of intra- and extra-fibrillar mineralization on the elastic properties of lamellar bone tissue through a multiscale finite element analysis. The results demonstrate that the overall mineral content in the tissue is the biggest contributor to the effective elastic properties of lamellar bone, and the extra-fibrillar matrix plays a primary role in the elastic response of the tissue.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Proceedings Paper
Engineering, Biomedical
Georgia S. Karanasiou, Panagiota I. Tsobou, Nikolaos S. Tachos, Luca Antonini, Lorenza Petrini, Giancarlo Pennati, Frank Gijsen, Farhad Rikhtegar Nezami, Rami Tzafiri, Ted Vaughan, Martin Fawdry, Dimitrios I. Fotiadis
42ND ANNUAL INTERNATIONAL CONFERENCES OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY: ENABLING INNOVATIVE TECHNOLOGIES FOR GLOBAL HEALTHCARE EMBC'20
(2020)