Review
Cell Biology
Zinia D'Souza, Farhana Taher Sumya, Amrita Khakurel, Vladimir Lupashin
Summary: The Golgi is the central organelle of the secretory pathway housing majority of the glycosylation machinery. Vesicular trafficking machinery plays a major role in coordinating Golgi trafficking for achieving Golgi homeostasis. Mutations in various components of glycosylation machinery can cause congenital disorders of glycosylation (CDG) with varying severities.
Article
Multidisciplinary Sciences
Ida Signe Bohse Larsen, Lorenzo Povolo, Luping Zhou, Weihua Tian, Kasper Johansen Mygind, John Hintze, Chen Jiang, Verity Hartill, Katrina Prescott, Colin A. Johnson, Sureni V. Mullegama, Allyn McConkie-Rosell, Marie McDonald, Lars Hansen, Sergey Y. Vakhrushev, Katrine T. Schjoldager, Henrik Clausen, Thomas Worzfeld, Hiren J. Joshi, Adnan Halim
Summary: Mutations in the TMEM260 gene cause heart defects and renal anomalies syndrome. The TMEM260 gene encodes an ER-located protein O-mannosyltransferase that selectively glycosylates IPT domains. Disease-causing TMEM260 mutations impair O-mannosylation of IPT domains and result in receptor matura-tion defects and abnormal growth of 3D cell models.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Chemistry, Physical
David Teze, Joan Coines, Folmer Fredslund, Kshatresh D. Dubey, Gonzalo N. Bidart, Paul D. Adams, John E. Dueber, Birte Svensson, Carme Rovira, Ditte H. Welner
Summary: The study found that UGT1 from Polygonum tinctorium can catalyze N-, O-, or S-glycosylation with similar rates, and elucidated the molecular basis of N-, O-, and S-specificities using experimental mutagenesis and simulations. It also showed that the active site can be engineered to increase or favor one of the three glycosylation activities over another, leading to the design of more active and specific enzyme variants for glycoside production.
Article
Multidisciplinary Sciences
Ping Lu, Yusong Liu, Maozhou He, Ting Cao, Mengquan Yang, Shutao Qi, Hongtao Yu, Haishan Gao
Summary: O-GlcNAcylation is an important post-translational modification that regulates cellular processes. The enzymes OGT and OGA mediate the addition and removal of O-GlcNAc on proteins. Cryo-EM structures reveal the mechanism by which OGT selects substrates and the mutual inhibition between OGT and OGA.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Saulius Vainauskas, Helene Guntz, Elizabeth McLeod, Colleen McClung, Cristian Ruse, Xiaofeng Shi, Christopher H. Taron
Summary: Characterizing mucin-type O-glycans linked to serine/threonine of glycoproteins is challenging due to limited enzymatic tools. This study demonstrated the effectiveness of the immunomodulating metalloprotease IMPa from Pseudomonas aeruginosa in cleaving peptides and proteins with various O-glycans, enabling comprehensive analysis without glycopeptide enrichment.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
He Zhu, Luyao Liu, Zheng Fang, Hongqiang Qin, Mingming Dong, Mingliang Ye
Summary: Different from N-linked glycosylation, the diversity and interpretation of O-linked glycans pose challenges. This study discovered Y-ion patterns in O-glycopeptide spectra, and proposed a search approach based on these patterns to identify O-glycopeptides. The approach constructs theoretical Y-ion patterns for matching experimental Y-ions, reducing search space and improving identification compared to existing software tools.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Noortje de Haan, Yoshiki Narimatsu, Mikkel Koed Moller Aasted, Ida S. B. Larsen, Irina N. Marinova, Sally Dabelsteen, Sergey Y. Vakhrushev, Hans H. Wandall
Summary: In this study, a high-throughput sample preparation method was developed for the nonreductive release and characterization of O-glycans from human cell materials. By using reducing-end labeling and mass spectrometry analysis, O-glycan structural isomers in complex mixtures were efficiently separated and detected. This method provides an opportunity to study glycomic changes in human tissue and disease models using mainstream analytical equipment.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hua Tang, Qiang Tang, Qian Zhang, Pengmian Feng
Summary: This study constructed a prediction model based on feature fusion to predict O-glycosites linked to threonine residues in human proteins. Random forest was selected as the final classifier and achieved high accuracy on both training and validation datasets. The developed webserver, O-GlyThr, assists glycobiologists in researching glycosylation structure and function.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Eugenia Wulff-Fuentes, Rex R. Berendt, Logan Massman, Laura Danner, Florian Malard, Jeet Vora, Robel Kahsay, Stephanie Olivier-Van Stichelen
Summary: Over the past 35 years, extensive research has been conducted on protein O-GlcNAcylation, resulting in the establishment of a database of human O-GlcNAcylated proteins and the acquisition of a more robust semi-consensus sequence through analysis. Additionally, a comprehensive meta-analysis of human O-GlcNAcylated proteins has revealed that O-GlcNAcylation is a master regulator of cell signaling.
Review
Endocrinology & Metabolism
Thomas J. McCorvie, Douglas Ferreira, Wyatt W. W. Yue, D. Sean Froese
Summary: Vitamin B-12 is an essential cofactor for two human enzymes, and its metabolism involves a complex network of transporters, enzymes, and chaperones. Recent studies have provided new insights into the structure and interactions of these proteins and how they regulate the absorption, transportation, and utilization of vitamin B-12. Future research combining structural and interactome-based approaches and considering post-translational modifications holds great promise for further understanding of vitamin B-12 metabolism.
JOURNAL OF INHERITED METABOLIC DISEASE
(2023)
Article
Biochemical Research Methods
Gurdeep Chahal, Medea Padra, Mattias Erhardsson, Chunsheng Jin, Macarena Quintana-Hayashi, Vignesh Venkatakrishnan, Janos Tamas Padra, Helen Stenback, Anders Thorell, Niclas G. Karlsson, Sara K. Linden
Summary: This study found that Helicobacter pylori infection in the stomach results in increased diversity and variation in glycan structures, primarily influenced by the binding ability of the BabA protein to fucosylated structures.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Multidisciplinary Sciences
Victor Taleb, Qinghua Liao, Yoshiki Narimatsu, Ana Garcia-Garcia, Ismael Companon, Rafael Junqueira Borges, Andres Manuel Gonzalez-Ramirez, Francisco Corzana, Henrik Clausen, Carme Rovira, Ramon Hurtado-Guerrero
Summary: This study provides molecular insights into the interaction between mucinase AM0627 and O-glycans in gut bacteria. The enzyme specifically cleaves bis-T O-glycopeptides by recognizing both the sugar moieties and the peptide sequence. Structural comparison reveals a conserved residue responsible for the common activity of AM0627 and another mucinase with bis-T/Tn substrates.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Andrea Zanotti, Joao P. L. Coelho, Dinah Kaylani, Gurdeep Singh, Marina Tauber, Manuel Hitzenberger, Donem Avci, Martin Zacharias, Robert B. Russell, Marius K. Lemberg, Matthias J. Feige
Summary: This study reveals a previously unknown function of the human signal peptidase complex (SPC) as a membrane protein quality control factor. The SPC cleaves membrane proteins at hidden cleavage sites when they fail to fold or assemble correctly, contributing to membrane protein homeostasis and cellular fitness.
Article
Chemistry, Analytical
Miloslav Sanda, Lindsay Morrison, Radoslav Goldman
Summary: The outbreak of the Covid-19 pandemic has caused the current global health crisis, prompting the need for detailed studies on SARS-CoV-2 proteins to develop effective antiviral compounds and vaccines. The glycosylation of the spike (S) protein plays a significant role in its proteolytic activation, expanding the current knowledge in this area.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Deva Saroja Talasila, Eike B. Bauer
Summary: The new strategy utilizing ferrocenium complexes as promoters has successfully activated glycosyl halide donors in glycosylation reactions, leading to high isolated yields and short reaction times, showing promising prospects for application.