Article
Chemistry, Analytical
Yue Wu, Yuyuan Zhou, Ratul Paul, Xiaochen Qin, Khayrul Islam, Yaling Liu
Summary: This study reports a microfluidic vessel-on-a-chip platform for recapitulating key metastatic steps of tumor cells in blood vessels and investigating the interactions between cells and vessels. Biomechanical factors like vascular surface properties and hemodynamics were found to have significant impacts on cell adhesion. Computational models based on confocal scanned 3D images were established to provide insights into tumor metastasis and inspire new ideas for anticancer therapy development.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Agnieszka Krakos (Podwin), Joanna Jarosz, Patrycja Sniadek, Mateusz Psurski, Adrianna Graja, Marcin Bialas, Ewa Oliszewska, Joanna Wietrzyk, Rafal Walczak, Jan Dziuban
Summary: This paper presents a lab-on-chip platform for the long-term cultivation of human cancer cells, which meets the requirements of CubeSat biological missions. Cancer cell lines UM-UC-3 and RT 112 were cultured on the chip for up to 50 days, showing successful results and providing a basis for further testing in terrestrial and space conditions.
Review
Biotechnology & Applied Microbiology
Faezeh Vakhshiteh, Zeinab Bagheri, Marziye Soleimani, Akram Ahvaraki, Parisa Pournemat, Seyed Ebrahim Alavi, Zahra Madjd
Summary: Nanomedicine is a promising therapeutic approach, but its clinical translation has been hindered by the lack of cellular models to predict tumor cell response. Heterotypic 3D tumor spheroids have become popular as a superior model for mimicking in vivo tumor heterogeneity and physiology. The stromal interactions in heterotypic 3D tumor spheroids can affect various aspects of cancer research, including response to therapy and nanomedicine penetration.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Zhengkun Chen, Sina Kheiri, Albert Gevorkian, Edmond W. K. Young, Valerie Andre, Ted Deisenroth, Eugenia Kumacheva
Summary: Skin cell organotypic micrometre-size 3D aggregates, known as multicellular spheroids, have potential to replace animal models in testing skincare products. A microfluidic platform for dermal fibroblast spheroids (DFSs) growth has been developed to screen active ingredients (AIs) like vitamin C efficiently. The computational simulation suggests that DFSs can serve as a reliable in vitro dermal model for skincare product screening.
Article
Multidisciplinary Sciences
Pei-Yu Chu, Han-Yu Hsieh, Pei-Shan Chung, Pai-We Wang, Ming-Chung Wu, Yin-Quan Chen, Jean-Chen Kuo, Yu-Jui Fan
Summary: The objective of this study is to develop a device that mimics the microfluidic system of human arterial blood vessels. The device combines fluid shear stress (FSS) and cyclic stretch (CS) resulting from blood flow and pressure. It enables real-time observation of dynamic morphological changes in cells under different flow fields and stretch. By understanding the effects of FSS and CS on endothelial cells (ECs), including alignment of cytoskeleton proteins with fluid flow and redistribution of paxillin, this study can contribute to the prevention and improvement of cardiovascular diseases.
Article
Biotechnology & Applied Microbiology
Qiao Xiong, Ting Liu, Yidie Ying, Xiaowen Yu, Ziwei Wang, Hongliang Gao, Tianhai Lin, Weihua Fan, Zhensheng Zhang, Qiang Wei, Yuqing Ge, Shuxiong Zeng, Chuanliang Xu
Summary: This study established a bladder cancer spheroids microfluidic drug sensitivity analysis system, which accurately assesses the response of bladder cancer to different chemotherapeutic agents and correlates with the clinical response of patients.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Polymer Science
Gulsim Kulsharova, Akbota Kurmangaliyeva, Elvira Darbayeva, Luis Rojas-Solorzano, Galiya Toxeitova
Summary: The study introduces a hybrid COC-PDMS microfluidic chip for successfully culturing Huh7 hepatoma cell line, showing potential for developing liver-on-a-chip platforms.
Article
Chemistry, Multidisciplinary
Umut Can Coskun, Funda Kus, Ateeq Ur Rehman, Berna Morova, Merve Gulle, Hatice Baser, Demet Kul, Alper Kiraz, Kemal Baysal, Ahmet Erten
Summary: Compared to traditional 2D cell cultures, 3D cell cultures provide a more clinically relevant cellular microenvironment. This study presents an easy-to-fabricate microfluidic platform called STICI3D that supports 3D cell cultures and enables precise control of the cellular microenvironment. The platform can be rapidly fabricated and mass manufactured, making it highly adaptable for researchers and those in need of ready-to-use plastic platforms.
Article
Pharmacology & Pharmacy
Valentina Palacio-Castaneda, Rik Oude Egberink, Arbaaz Sait, Lea Andree, Benedetta Maria Sala, Negar Hassani Besheli, Egbert Oosterwijk, Johan Nilvebrant, Sander C. G. Leeuwenburgh, Roland Brock, Wouter P. R. Verdurmen
Summary: Investigating drug delivery using a versatile microfluidic platform demonstrates specific targeting of proteins and mRNA, showing potential for bone defect regeneration in a 3D culture system.
Article
Multidisciplinary Sciences
Naoyuki Takahashi, Daisuke Yoshino, Ryuji Sugahara, Satomi Hirose, Kazuki Sone, Jean-Paul Rieu, Kenichi Funamoto
Summary: This study developed a microfluidic platform for reconstructing hypoxic vascular microenvironments, by simultaneously applying hypoxic stress and fluid shear stress. The results showed that under the simultaneous exposure to hypoxic stress and fluid shear stress, the migration velocity of endothelial cells increased and gradually decreased, reaching the lowest value. Meanwhile, the cells aligned and elongated in the flow direction, with enhanced expression of VE-cadherin and actin filament assembly. Therefore, this microfluidic platform is useful for investigating the dynamics of endothelial cells in vascular microenvironments.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Ece Yildiz-Ozturk, Pelin Saglam-Metiner, Ozlem Yesil-Celiktas
Summary: This study successfully developed a lung carcinoma spheroid-based microfluidic platform with perfusion function to mimic lung cancer pathology and investigate the effect of the potential drug molecule panaxatriol. Results showed that panaxatriol exhibited higher cytotoxic effect on cancer cells, with an IC50 value of 61.55 µM. Panaxatriol was more effective on single cells around the spheroid structure, but less effective in 3D spheroid tissues with a compact structure in static conditions compared to dynamic systems.
Review
Medicine, Research & Experimental
Leah Susser, Katey J. Rayner
Summary: Cardiovascular disease is the leading cause of death worldwide, with coronary artery disease being the most common underlying condition. CVDs are now recognized as chronic inflammatory conditions, with arterial blockages and atherosclerosis being key factors. Macrophages play a crucial role in the progression and regression of atherosclerosis.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Chemistry, Multidisciplinary
Xiongfa Ji, Ho-Pan Bei, Guoqing Zhong, Hongwei Shao, Xuecheng He, Xin Qian, Yu Zhang, Xin Zhao
Summary: Primary cancer influences the bone microenvironment to induce dormancy and metastasis in tumor cells, leading to organ metastasis and death. In this study, a bone-on-a-chip (BOC) platform is created using 3D printing technology to mimic the pre-metastatic niches (PMNs), allowing investigation of the interactions between bone-resident cells and metastatic tumor cells under the influence of primary cancer. The platform utilizes a photocrosslinkable gelatin methacrylate (GelMA) hydrogel for 3D cell culture, combined with circulating tumor culture medium (CM) to examine the role of mesenchymal stem cells (MSCs) and osteoclasts (RAW264.7s). Three niches representing dormancy, perivascular, and vicious cycle are designed to simulate bone metastasis on a single chip with high cell viability and nutrient exchange. The platform is demonstrated to be functional and practical, providing insights into PMN formation and potential drug discovery.
Article
Chemistry, Analytical
Dong Hee Kang, Na Kyong Kim, Sang-Woo Park, Hyun Wook Kang
Summary: FLISA is a commonly used, quantitative technique for detecting biochemical changes based on antigen-antibody binding reactions using a well-plate platform. The proposed microfluidic system with a three-dimensional incubation chamber allows for reduced reagent usage and faster processing time. The system achieves antigen-antibody binding reactions and the binding of fluorogenic substrates to target proteins.
Article
Engineering, Biomedical
B. Lucendo-Villarin, J. Meseguer-Ripolles, J. Drew, L. Fischer, E. Ma, O. Flint, K. J. Simpson, L. M. Machesky, J. C. Mountford, D. C. Hay
Summary: Liver disease is a growing global health problem, with liver transplant as the only curative treatment for end-stage liver disease, but donor organs are insufficient. In order to address the limitations of current cell-based models in performance and cost, the authors have developed an automated platform to scale up the production of liver tissue for disease modeling and drug screening.
Article
Engineering, Mechanical
Wenbo Shi, Zhousuo Zhang
Summary: This study presents a new contact characteristic parameters model for bolted joint interfaces, which overcomes the shortcomings of homogeneous distributions of contact stiffness and damping in previous models. By transforming the contact pressure distribution into normal contact load distribution, it is found that the interfacial contact stiffness and damping exhibit significant inhomogeneous distributions.
TRIBOLOGY INTERNATIONAL
(2022)
Article
Chemistry, Multidisciplinary
Xiao-lian Liu, Gui-ming Zhang, Si-si Huang, Wen-hui Shi, Lin-xuan Ye, Zhong-lu Ren, Jia-jie Zhang, Shu-wen Liu, Le Yu, Yi-lei Li
Summary: PTEN loss correlates with hypersensitivity to rapalogs in ccRCC cells and could be a marker for rapalog therapy selection in ccRCC patients.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Thermodynamics
Mengdi Cui, Baolong Wang, Falin Wei, Wenxing Shi
Summary: The study proposes a modified exergy analysis method to locate major imperfections and determine optimization directions for developing high-efficiency vapor compression systems, achieving significant improvements in coefficient of performance for air source heat pump water heaters and room air conditioners through improving energy efficiency of compressors and decreasing refrigerant exergy destruction.
APPLIED THERMAL ENGINEERING
(2022)
Article
Engineering, Environmental
Chuang Wang, Jinying Du, Zhijie Liang, Jialiang Liang, Zhiwei Zhao, Fuyi Cui, Wenxin Shi
Summary: The study investigated the removal efficiency and mechanism of fluoroquinolones using the VUV/Fe2+/PMS process, showing excellent performance at wide initial pH range. The degradation pathways of fluoroquinolones and main inorganic byproducts were thoroughly studied in this synergistic activation process.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Chemical
Bing Zhang, Xin Mao, Xiaomin Tang, Heli Tang, Bing Zhang, Yu Shen, Wenxin Shi
Summary: This study compares the application of modified microbial flocculants (MMFs) with conventional organic flocculants in mitigating membrane fouling. The results show that MMF has better performance in pollutant removal and membrane fouling control. The study also reveals that cake layer formation is the main mechanism of membrane fouling and MMF can reduce the accumulation of foulants on the membrane surface. Correlation analysis indicates that total phosphorus, polysaccharide, zeta potential, and particle size have the greatest correlation with fouling resistance.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Environmental Sciences
Zhanhua Zhou, Kim Yrjala, Junhui Chen, Chenliang Yu, Wenhui Shi, Hua Qin, Weiwu Yu, Wensheng Dai, Yuanyuan Hu, Jiasheng Wu
Summary: The combination of biochar and sheep manure significantly improved soil fertility and plant growth in a Torreya grandis plantation, demonstrating significant effects on plant quality and soil nutrients when compared to individual treatments with manure or rapeseed cake.
JOURNAL OF SOILS AND SEDIMENTS
(2022)
Article
Chemistry, Analytical
Tyler Chozinski, B. Scott Ferguson, William Fisher, Shencheng Ge, Qiang Gong, Hui Kang, John McDermott, Alexander Scott, Wentao Shi, Jeremiah J. Trausch, Thorsten Verch, Matthew Vukovich, Jinpeng Wang, J. Emma Wu, Qin Yang
Summary: This study aims to develop a portable device for affinity assays of viral particles in viral vaccines by combining aptamers, microfluidic devices, and electrochemical detection. By generating and characterizing aptamers and designing/fabricating electrochemical microfluidic devices, CMV concentrations were successfully measured. This work represents a critical first step towards developing simple, affordable, and robust affinity assays for global deployment.
ANALYTICAL CHEMISTRY
(2022)
Article
Acoustics
Wentao Shi, Xiangshui Li, Weidong Wang, Weijie Tan, Hui Li
Summary: In this article, a sparse alternating iterative minimization (SAIM) method is proposed to address the direction-of-arrival (DOA) estimation problem via a non-ideal acoustic vector hydrophone (AVH) array. The method establishes a non-ideal AVH array model and formulates a new cost function to provide accurate DOA estimation. Extensive numerical simulation and experimental results demonstrate the superior DOA estimation performance of the proposed method.
Article
Engineering, Environmental
Bijiang Geng, Shirui Zhang, Xue Yang, Wenyan Shi, Ping Li, Dengyu Pan, Longxiang Shen
Summary: This study developed a novel heterojunction nanosonosensitizer with enhanced production of singlet oxygen and hydroxyl radicals for sonodynamic therapy. The nanosonosensitizer effectively separated the electron-hole pairs generated by ultrasound through a direct Z-scheme charge transfer mechanism. The experiments demonstrated complete tumor eradication in osteosarcoma models using a single drug injection and single ultrasound irradiation.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Pharmacology & Pharmacy
Wentao Shi, Manali Singha, Gopal Srivastava, Limeng Pu, J. Ramanujam, Michal Brylinski
Summary: Computational modeling is crucial in modern drug discovery, particularly in predicting binding molecules. Pocket2Drug is a promising computational approach that uses data mining and machine learning to predict binding molecules for a given ligand binding site.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Crystallography
Shuai Yue, Yushuai Bai, Zhonghua Du, Huaiwu Zou, Wenhui Shi, Guang Zheng
Summary: This study aims to investigate the dynamic response associated with the impact of a kinetic projectile on the internal structure of an artificial satellite. Through ground damage testing and finite element dynamic simulation, we determined the lateral effect and penetration performance of the projectile, and studied its dynamic behavior when penetrating a honeycomb sandwich panel and multi-layer plates. The ballistic limit velocity of the projectile was found to be 150 m/s, and the deformation of the opening had little relationship with the projectile velocity. Additionally, it was observed that the launch velocity required to meet the damage requirements of the multi-layer plates was within 325 +/- 25 m/s, with higher initial velocities resulting in reduced damage area.
Article
Immunology
Mingyue Cai, Wensou Huang, Jingjun Huang, Wenbo Shi, Yongjian Guo, Licong Liang, Jingwen Zhou, Liteng Lin, Bihui Cao, Ye Chen, Juan Zhou, Kangshun Zhu
Summary: TACE-L-P significantly improves survival in advanced HCC patients with acceptable safety, especially in those without main portal vein invasion but with extrahepatic metastasis or tumor number >3.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Surgery
Haifeng Wang, Wei Xue, Weigang Yan, Lei Yin, Baijun Dong, Biming He, Yongwei Yu, Wentao Shi, Zhien Zhou, Hengzhi Lin, Yi Zhou, Yanqing Wang, Zhenkai Shi, Shancheng Ren, Xu Gao, Linhui Wang, Chuanliang Xu
Summary: High-frequency irreversible electroporation (H-FIRE) is a promising treatment for localized prostate cancer. It can selectively destroy cancer cells while preserving surrounding nerves and blood vessels. The study showed that H-FIRE had a lower rate of clinically significant prostate cancer at 6 months compared to other energy platforms.
Article
Materials Science, Multidisciplinary
Yaxi Tian, Dongyun Que, Rongfeng Guan, WenYan Shi
Summary: Cu-BiVO4 photocatalysts with excellent visible light response were successfully fabricated by modification with a SiO2 template and Cu doping. Cu doping can reduce carrier recombination rate and greatly improve the ability of BiVO4 to degrade RhB. The crystal phase and photocatalytic activity of Cu-BiVO4 are influenced by doping amount. The mechanism of Cu doping was investigated using first-principles calculations.
MATERIALS TODAY COMMUNICATIONS
(2022)
Article
Business, Finance
Wen Shi, Xiaogang Bi
Summary: This research examines the impact of Buddhism on mergers & acquisitions (M&As) performance using data from 4622 M&A transactions of Chinese public firms between 2004 and 2018. The findings provide ample evidence that acquirers located in regions with a higher intensity of Buddhism achieve better short-term and long-term performance after the merger. Furthermore, the study reveals that state ownership plays a crucial role in mitigating this effect, and the value creation is driven by higher deal completion rates and fewer violation cases for acquiring firms. Finally, the study addresses the issue of endogeneity, and the results remain unchanged.
ACCOUNTING AND FINANCE
(2023)