Article
Cell Biology
Abigail A. Kindberg, Vasudha Srivastava, Jonathon M. Muncie, Valerie M. Weaver, Zev J. Gartner, Jeffrey O. Bush
Summary: The study reveals that EPH/EPHRIN signaling decreases the stability of heterotypic cell:cell contacts through increased cortical actomyosin contractility, driving cellular self-organization. The cell segregation driven by EPH/EPHRIN depends on actomyosin contractility but occurs independently of directed cell migration and without changes in cell adhesion.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Physics, Fluids & Plasmas
Satoru Okuda, Tetsuya Hiraiwa
Summary: In this study, a continuum model was constructed to describe long-term adherent cell dynamics in three-dimensional space. The model reproduced the dynamic behaviors of adherent cells on a substrate and discovered more complex cell slipping and membrane flow patterns. The proposed model has the potential to analyze the mechanical behavior of cell adhesion in long-term dynamics.
Review
Cell Biology
Brenda Canales Coutino, Roberto Mayor
Summary: This article discusses the mechanical forces exerted on neural crest cells during migration and differentiation and describes proteins with mechanosensitive functions in other systems. It proposes future studies to elucidate the mechanosensitive functions of these receptors in neural crest development.
DEVELOPMENTAL CELL
(2022)
Article
Chemistry, Multidisciplinary
Qiongyu Li, Yixuan Xie, Rachel Rice, Emanual Maverakis, Carlito B. Lebrilla
Summary: Antigen-antibody proximity labeling (AAPL) is a novel method to map the antigen interaction sites as well as interactors of antibody-targeted proteins. Oxidative proteomic analysis can determine the degree of oxidation, the site of oxidation within the targeted antigen, and the interacting proteins that were in close proximity to the targeted antigen.
Article
Multidisciplinary Sciences
Shigetaka Nishiguchi, Rinshi S. Kasai, Takayuki Uchihashi
Summary: Cadherin EGF LAG seven-pass G-type receptors (CELSR) cadherins and adhesion G-protein-coupled receptors are crucial for cell-cell adhesion and the establishment of planar cell polarity. This study investigated the binding mode of CELSR cadherins and found that the trans-dimer of CELSR cadherin is the essential adhesive unit between cells. The analysis results also showed that EC1-EC8 overlap and twist to form antiparallel dimer conformations, and the binding of EC1-EC4 is sufficient for bead aggregation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Anne-Florence Blandin, Elisabete Cruz Da Silva, Marie-Cecile Mercier, Oleksandr Glushonkov, Pascal Didier, Stephane Dedieu, Cristophe Schneider, Jessica Devy, Nelly Etienne-Selloum, Monique Dontenwill, Laurence Choulier, Maxime Lehmann
Summary: The study showed that EGFR TKIs induce increased EGFR accumulation in early endosomes in GBM cells, potentially altering the response of glioma cells to therapeutic treatment. Depletion of integrins can also affect the sensitivity of glioma cells to tyrosine kinase inhibitors.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Dan Tian, Qing Qin, Mingfei Li, Xiaoyu Li, Qing Xu, Qianzhou Lv
Summary: This study found significantly elevated levels of EphA2, VCAM-1, and Hcy in patients with arteriosclerosis, with EphA2 knockdown leading to impaired cell adhesion and migration. Furthermore, the interaction between PGRN and EphA2 signaling pathway may play a crucial role in Hcy-induced endothelial injury.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Alejandra Matamoros-Recio, Juan Felipe Franco-Gonzalez, Lucia Perez-Regidor, Jean-Marc Billod, Joan Guzman-Caldentey, Sonsoles Martin-Santamaria
Summary: This study provides a realistic and complete 3D model of the TLR4 complex, revealing its behavior in different membrane environments, offering insights into the signal transduction mechanism, and promoting the discovery of new TLR4 modulators.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Multidisciplinary Sciences
Bin Xie, Allison Maker, Andrew Priest, David M. Dranow, Jenny N. Phan, Thomas E. Edwards, Bart L. Staker, Peter J. Myler, Barry M. Gumbiner, Sanjeevi Sivasankar
Summary: The monoclonal antibody 19A11 can interact with Ecad in two distinct modes, one of which enhances the adhesion of Ecad. Adhesion is strengthened by the formation of a salt bridge.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Christopher Mendoza, Sai Harsha Nagidi, Dario Mizrachi
Summary: The JAM family, members of the immunoglobulin superfamily, each have unique tertiary structures despite similar secondary structures; heterotypic interactions among JAM family members may be greatly favored compared to homotypic interactions; homotypic or heterotypic interactions among JAMs are stronger than that of E-CADs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Di Wu, Kenneth M. Yamada, Shaohe Wang
Summary: Multicellular organisms generate diverse tissues through interactions between cells and the extracellular matrix. Cells make decisions based on chemical and mechanical information from their environment, which in turn modifies the tissue morphology.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Jake Burton, Jesus A. Siller-Farfan, Johannes Pettmann, Benjamin Salzer, Mikhail Kutuzov, P. Anton van der Merwe, Omer Dushek
Summary: Chimeric antigen receptors (CARs) have lower sensitivity to antigens presented on antigen-presenting cells (APCs) compared to T cell receptors (TCR), but nearly identical sensitivity to purified protein antigens. Engaging CD2 or LFA-1 can significantly improve TCR sensitivity, but has minimal effect on CAR sensitivity. Fusion of CAR variable domains to the TCR CD3 epsilon subunit (TRuC) or exchanging TCR alpha beta variable domains for those of the CAR (STAR or HIT) can partially or fully restore antigen sensitivity, respectively, by enhancing their ability to exploit CD2 and LFA-1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Robert M. Harmon, John Devany, Margaret L. Gardel
Summary: This study investigates the mechanism by which the actin assembly factor Dia1 guides cell positioning in multicellular tissues. The results suggest that Dia1 promotes rapid growth of cell-cell adhesions and plays a crucial role in the construction of a highly crowded monolayer. The study also finds a close correlation between Dia1 expression patterns, cell stratification, and differentiation.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Andres Martin Toscani, Pablo Aguilera, Federico Coluccio Leskow
Summary: The physical interaction between ErbB2 and DDR1 receptors in breast cancer cells suggests their involvement in the signaling pathway. These receptors are coexpressed in normal mammary gland but not in breast tumors.
Review
Cell Biology
Amanda Haage, Archana Dhasarathy
Summary: Cells are able to sense environmental changes, transmit signals, and influence adaptive changes in cell behavior. Mechanosensing, involving various macromolecular structures, plays a role in integrating physical stimuli and regulating cellular behavior. The extracellular matrix and cell adhesion proteins are crucial components of the cellular microenvironment, and their disruption can lead to disease. Recent advances in microscopy and genomic technologies have enhanced our understanding of the impact of nuclear localization of adhesion proteins. This review will cover the migration of adhesion proteins to the nucleus and their downstream functions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biology
Zherui Xiong, Harriet P. Lo, Kerrie-Ann Mcmahon, Nick Martel, Alun Jones, Michelle M. Hill, Robert G. Parton, Thomas E. Hall
Summary: This method utilizes proximity-dependent biotin labelling in zebrafish to conduct tissue-specific proteomic profiling, demonstrating its applicability in diverse cell types. Using this system, specific interaction networks within muscle cells for closely related Cavin4 and Cavin1 proteins were successfully defined.
Article
Multidisciplinary Sciences
Xuan Sun, Benjamin Cao, Marina Naval-Sanchez, Tony Pham, Yu Bo Yang Sun, Brenda Williams, Shen Y. Heazlewood, Nikita Deshpande, Jinhua Li, Felix Kraus, James Rae, Quan Nguyen, Hamed Yari, Jan Schroder, Chad K. Heazlewood, Madeline Fulton, Jessica Hatwell-Humble, Kaustav Das Gupta, Ronan Kapetanovic, Xiaoli Chen, Matthew J. Sweet, Robert G. Parton, Michael T. Ryan, Jose M. Polo, Christian M. Nefzger, Susan K. Nilsson
Summary: Nicotinamide riboside treatment can restore metabolic capacity of hematopoietic stem cells through various ways and shift aged HSC function, metabolism, and molecular characteristics towards a youthful state.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Kai-En Chen, Qian Guo, Timothy A. Hill, Yi Cui, Amy K. Kendall, Zhe Yang, Ryan J. Hall, Michael D. Healy, Joanna Sacharz, Suzanne J. Norwood, Sachini Fonseka, Boyang Xie, Robert C. Reid, Natalya Leneva, Robert G. Parton, Rajesh Ghai, David A. Stroud, David P. Fairlie, Hiroaki Suga, Lauren P. Jackson, Rohan D. Teasdale, Toby Passioura, Brett M. Collins
Summary: The retromer complex plays a crucial role in endosomal membrane trafficking and signaling. Novel macrocyclic peptides have been developed to modulate its function by specifically binding to different subunits and disrupting interactions with other factors. These peptides provide insights into retromer function and offer a new therapeutic avenue for targeting this complex.
Article
Cell Biology
Amelia L. Parker, Wee Siang Teo, Simon Brayford, Ullhas K. Moorthi, Senthil Arumugam, Charles Ferguson, Robert G. Parton, Joshua A. McCarroll, Maria Kavallaris
Summary: Beta III-tubulin, aberrantly expressed in epithelial cancers, impacts mitochondrial volume and shape through its C-terminal tail. It interacts specifically with regulators of mitochondrial dynamics and promotes a dynamic and extended mitochondrial network. The regulatory role of Beta III-tubulin in mitochondrial network dynamics has implications for maintaining mitochondrial homeostasis in health and disease.
Editorial Material
Multidisciplinary Sciences
Robert G. Parton, Brett M. Collins
Summary: The structure of caveolin, the major membrane protein of caveolae, is revealed by cryo-electron microscopy, providing new insights into its functions.
Article
Cell Biology
Danyon Harkins, Tracey J. Harvey, Cooper Atterton, Ingrid Miller, Laura Currey, Sabrina Oishi, Maria Kasherman, Raul Ayala Davila, Lucy Harris, Kathryn Green, Hannah Piper, Robert G. Parton, Stefan Thor, Helen M. Cooper, Michael Piper
Summary: The transcription factor NFIX plays a pivotal role in the regulation of cell adhesion within ependymal cells of the lateral ventricles, with Nfix(-/-) mice exhibiting abnormal cilia structure and disrupted localization of adhesion proteins.
Review
Pharmacology & Pharmacy
Gareth Griffiths, Jean Gruenberg, Mark Marsh, Jens Wohlmann, Arwyn T. Jones, Robert G. Parton
Summary: Nanoparticles are promising for delivering drugs, proteins, and nucleic acids into cells. The development and application of nanoparticles involve scientists from various fields who design, fabricate, and characterize nanoparticles in vitro. However, the research on the interactions between nanoparticles and cells in the field of cell biology is often of sub-standard quality.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Correction
Cell Biology
Paulina Moreno-Layseca, Niklas Z. Jantti, Rashmi Godbole, Christian Sommer, Guillaume Jacquemet, Hussein Al-Akhrass, James R. W. Conway, Pauliina Kronqvist, Roosa E. Kallionpaa, Leticia Oliveira-Ferrer, Pasquale Cervero, Stefan Linder, Martin Aepfelbacher, Henrik Zauber, James Rae, Robert G. Parton, Andrea Disanza, Giorgio Scita, Satyajit Mayor, Matthias Selbach, Stefan Veltel, Johanna Ivaska
NATURE CELL BIOLOGY
(2022)
Article
Cell Biology
Elin Larsson, Bjoern Moren, Kerrie-Ann McMahon, Robert G. Parton, Richard Lundmark
Summary: Research reveals that the large GTPase dynamin 2 does not mediate caveolae fission from the plasma membrane, as previously thought. Instead, dynamin 2 associates with a subset of caveolae and restrains their presence on the plasma membrane via a GTPase-independent mechanism.
JOURNAL OF CELL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yeping Wu, Ye-Wheen Lim, Robert G. Parton
Summary: Oxidative stress is a common feature in many diseases, but cells have evolved complex mechanisms to respond to this stress. Recent studies suggest that oxidative stress can cause disassembly of caveolae, releasing caveola proteins that regulate redox balance in the cell. These studies highlight the crucial role of caveolae in maintaining cellular susceptibility to oxidative stress-induced cell death and in cellular homeostasis and wound healing.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2023)
Article
Cell Biology
Yeping Wu, Ye-Wheen Lim, David A. Stroud, Nick Martel, Thomas E. Hall, Harriet P. Lo, Charles Ferguson, Michael T. Ryan, Kerrie-Ann McMahon, Robert G. Parton
Summary: Using quantitative whole-cell proteomics, researchers found that the oxidative stress response is the major pathway dysregulated in cells lacking the caveola structural protein CAVIN1. CAVIN1 deletion compromised sensitivity to oxidative stress and wound-induced reactive oxygen species accumulation, affecting regeneration. Oxidative stress triggered lipid peroxidation and caveolar disassembly, allowing release of CAVIN1 and direct interaction with NRF2, resulting in NRF2 degradation and resistance to lipid-peroxidation-induced ferroptosis in CAVIN1-null cells.
DEVELOPMENTAL CELL
(2023)
Review
Immunology
Remi Safi, Miguel Sanchez-Alvarez, Marta Bosch, Caroline Demangel, Robert G. Parton, Albert Pol
Summary: Microbes have developed strategies to exploit lipid droplets (LDs), which are major lipid storage organelles in eukaryotes, as a source of nutrients for host colonization. However, recent studies have shown that LDs possess protein-mediated antibiotic activity, which is upregulated in response to danger signals and sepsis. LDs may serve as a chokepoint utilized by the innate immune system to defend against intracellular pathogens.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Multidisciplinary Sciences
Azelle Hawdon, Niall D. Geoghegan, Monika Mohenska, Anja Elsenhans, Charles Ferguson, Jose M. Polo, Robert G. Parton, Jennifer Zenker
Summary: The spatial sorting of RNA transcripts in mouse preimplantation embryos plays a crucial role in gene expression and cell fate determination. Basally directed RNA transport is facilitated by microtubules and lysosomes. Despite higher accumulation in basal regions, higher translation activity occurs at the dispersed apical RNA foci, demonstrating spatial heterogeneities in RNA subtypes, organelle interactions, and translation events. Biased inheritance of RNA transcripts and differential translation capacity regulate cell fate allocation during embryonic development.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Christopher J. Peddie, Christel Genoud, Anna Kreshuk, Kimberly Meechan, Kristina D. Micheva, Kedar Narayan, Constantin Pape, Robert G. Parton, Nicole L. Schieber, Yannick Schwab, Benjamin Titze, Paul Verkade, Aubrey Weigel, Lucy M. Collinson
Summary: Volume electron microscopy (vEM) is an emerging technique that allows deep imaging of cellular and tissue structures. This article provides an introduction to the different vEM imaging modalities, specialized sample processing, and image analysis pipelines, showcasing key applications in the biosciences.
NATURE REVIEWS METHODS PRIMERS
(2022)
Correction
Multidisciplinary Sciences
Christopher J. Peddie, Christel Genoud, Anna Kreshuk, Kimberly Meechan, Kristina D. Micheva, Kedar Narayan, Constantin Pape, Robert G. Parton, Nicole L. Schieber, Yannick Schwab, Benjamin Titze, Paul Verkade, Aubrey Weigel, Lucy M. Collinson
NATURE REVIEWS METHODS PRIMERS
(2022)
