Article
Materials Science, Ceramics
Yessie W. Sari, Annisa Tsalsabila, Noviyan Darmawan, Yuliati Herbani
Summary: This study investigated the effects of surface-modified gold nanoparticles with different amino acids on the formation of hydroxyapatite. The results showed that aspartic acid modification of the gold nanoparticles promoted the crystallization of hydroxyapatite with a smaller crystallite size and a higher Ca/P ratio, which is beneficial for bone substitute applications.
CERAMICS INTERNATIONAL
(2022)
Article
Materials Science, Ceramics
Yessie W. Sari, Annisa Tsalsabila, Angga Saputra, Nur Aisyah Nuzulia, Yuliati Herbani
Summary: Surface modification of biomaterials affects the surface reactivity of implantable materials and induces the formation of apatite-like layers. This study investigates the bioactivity and surface reactivity of modified biomaterials (calcium-deficient hydroxyapatite [CDHA] on amino acid-capped AuNPs [AA-AuNPs]) by immersing them in simulated body fluid (SBF). The results show that AA-AuNPs, capped with charged and polar uncharged amino acids, are capable of inducing biomimetic hydroxyapatite formation. The presence of hydroxyapatite peaks in XRD patterns confirms the formation of hydroxyapatite after immersion in SBF. Furthermore, longer immersion time leads to nucleation and growth of hydroxyapatite, as indicated by changes in crystal structure. The surface modification of AA-AuNPs significantly affects the orientation of hydroxyapatite crystals and the presence of charged amino acids contributes to nucleation and growth, while polar uncharged amino acids only contribute to growth. The study highlights the bioactivity of CDHA-modulated AA-AuNPs in hydroxyapatite formation for bone tissue regeneration.
CERAMICS INTERNATIONAL
(2023)
Article
Chemistry, Multidisciplinary
Phil Opitz, Laura Besch, Martin Panthoefer, Anke Kabelitz, Ronald E. Unger, Franziska Emmerling, Mihail Mondeshki, Wolfgang Tremel
Summary: This study reports a protein-free formation of bone-like apatite from amorphous precursors through ball-milling. Mg2+ ions are crucial to achieve complete amorphization of CaCO3, promoting cell growth through stimulating cell viability ex vivo.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Yuchen Zhang, Tianyu Shu, Silin Wang, Zhongbo Liu, Yilong Cheng, Ang Li, Dandan Pei
Summary: The report reviewed the complex and tight relationship between bone healing response and CaP-based biomaterials, with emphasis on in vivo degradation mechanisms of such materials and their osteoinductive properties mediated by immune responses, osteoclastogenesis and osteoblasts. A deep understanding of the interaction between biological healing process and biomaterials is crucial for optimizing the design of CaP-based biomaterials and effective strategies for customization.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Yuchen Zhang, Shaoyang Ma, Jiaming Nie, Zhongbo Liu, Faming Chen, Ang Li, Dandan Pei
Summary: Bone mineralization is a complex process involving the interplay between organic and inorganic components of bone tissues. This review focuses on the evolution of mineral precursors in the bone mineralization process and highlights the tight control organisms have over each step. The connection between biomineralization and biomaterial design strategies is discussed, along with the detailed implementation of mineral precursor-based biomaterials in various bone graft materials.
Article
Engineering, Biomedical
Annika Seifert, Tina Tylek, Carina Blum, Naomi Hemmelmann, Bettina Boettcher, Uwe Gbureck, Juergen Groll
Summary: This study investigated the response of human macrophages to different CaPs and pore structures. The study found that macrophages are capable of releasing ETs in biomaterials, and METs associated with calcium deficient hydroxyapatite were significantly weaker or absent.
Review
Engineering, Biomedical
Ilijana Kovrlija, Janis Locs, Dagnija Loca
Summary: Octacalcium phosphate (OCP) has significant advantages as a precursor to biologically formed apatite and is utilized as a carrier for biologically active substances in bone regeneration. However, research on drug delivery systems with OCP is still in its pioneering stage, and comprehensive data is lacking to fully understand the benefits it can bring. Further studies are needed to explore the potential of OCP in drug delivery systems for bone tissue engineering.
ACTA BIOMATERIALIA
(2021)
Article
Materials Science, Ceramics
Y. W. Sari, A. Saputra, A. Bahtiar, N. A. Nuzulia
Summary: This paper investigates the effects of microwave irradiation treatment on the properties of nanohydroxyapatite (nanoHAP) materials, highlighting the advantages of rapid heating, reduced reaction time, and increased productivity. Microwave irradiation is shown to facilitate the transformation of eggshell calcium into nanoHAP and the production of smaller particles with extended irradiation. Toxicity analysis reveals high cell viability of human embryonic cells after exposure to nanoHAP obtained from microwave irradiation treatment, indicating its potential as a biomaterial.
CERAMICS INTERNATIONAL
(2021)
Article
Biology
Petra Wolint, Lukas Naf, Desiree Schibler, Nora Hild, Wendelin J. Stark, Pietro Giovanoli, Maurizio Calcagni, Johanna Buschmann
Summary: The study investigates the use of calcium phosphate nanoparticles to induce stem cells towards bone cell differentiation, showing potential for osteogenic and endothelial commitment.
Article
Biochemistry & Molecular Biology
Masako Fujioka-Kobayashi, Hiroki Katagiri, Niklaus P. Lang, Jean-Claude Imber, Benoit Schaller, Nikola Saulacic
Summary: The aim of this study was to investigate the effect of synthetic bone substitutes in combination with deproteinized bovine bone mineral on bone formation. The results showed that the combination of alpha-TCP and DBBM resulted in similar levels of new bone formation compared to the negative control. Micro-CT analysis indicated resorption of the combined materials, but without any change in volume.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Marcio H. Nogueira, Lucile Humblot, Raghvendra Pratap Singh, Emilie Dieude-Fauvel, Bertrand Doumert, Sarah Nasser, Celine Lesur, Romdhane Karoui, Guillaume Delaplace, Paulo P. S. Peixoto
Summary: The study found that the removal of CCPs affects the structural modification of casein micelle, but the loss of CCPs is not always equivalent to the reduction in total calcium and phosphorous ions. Demineralization results in the total depletion of some CCPs, while the decrease in Hard regions is not directly correlated to the decrease in CCPs.
FOOD HYDROCOLLOIDS
(2021)
Article
Biochemistry & Molecular Biology
Yu-Hsun Chiu, I-Cheng Chen, Chen-Ying Su, Hsin-Hua Tsai, Tai-Horng Young, Hsu-Wei Fang
Summary: The demand for bone grafting is increasing as the global population ages. This study aims to develop ideal injectable implants from biomaterials for minimally invasive surgery. The developed injectable paste showed good handling properties, biocompatibility, and mechanical strength.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Green & Sustainable Science & Technology
Mohd Faiz Muaz Ahmad Zamri, Raihana Bahru, Rashid Amin, Muhammad Umar Aslam Khan, Saiful Izwan Abd Razak, Shukur Abu Hassan, Mohammed Rafiq Abdul Kadir, Nadirul Hasraf Mat Nayan
Summary: The utilization of waste-derived materials in tissue engineering applications shows outstanding biocompatibility and biodegradability, contributing to environmental sustainability by reducing waste disposal, increasing source availability, and minimizing manufacturing cost. The desirable properties and potentials of waste materials create significant opportunities for their implementation in tissue engineering, as summarized in recent research on the subject.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Chemistry, Multidisciplinary
Shuqin Jiang, Yuanyuan Cao, Chenxi Zong, Yuanfeng Pang, Zhiwen Sun
Summary: The addition time of Mg2+ plays a crucial role in the regulation of amorphous calcium phosphate-mediated hydroxyapatite crystallization. Adding Mg2+ before or during ACP formation inhibits HAP crystallization effectively, while adding it in the unstable phase has no effect. Surface associated Mg2+ on ACP is shown to be more suppressive to HAP crystallization than Mg2+ incorporation.
Article
Chemistry, Multidisciplinary
Md. Sahadat Hossain, Samina Ahmed
Summary: Hydroxyapatites were synthesized in amorphous and crystalline phases by sintering calcium carbonate and ortho-phosphoric acid at temperatures ranging from 300 to 1100 degrees C. Fourier transformation infrared (FTIR) spectra revealed variations in the asymmetric and symmetric stretching, and bending vibrations of phosphate and hydroxyl groups. The peak intensities at specific wavenumbers gradually increased with higher sintering temperatures, and peak separations were observed at certain wavenumbers when the temperature reached 700 degrees C or higher. X-ray diffraction (XRD) was also used to investigate the crystalline and amorphous phases of the synthesized hydroxyapatites.
Article
Materials Science, Biomaterials
Rita Gelli, Gemma Di Pompo, Gabriela Graziani, Sofia Avnet, Nicola Baldini, Piero Baglioni, Francesca Ridi
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Article
Chemistry, Physical
Rita Gelli, Monica Tonelli, Francesca Ridi, Massimo Bonini, Habauka M. Kwaambwa, Adrian R. Rennie, Piero Baglioni
Summary: The study investigated the effect of proteins extracted from Moringa oleifera seeds on the stability and crystallization pathway of endogenous amorphous magnesium-calcium phosphates in the human body. It was found that these proteins effectively delayed the transition from amorphous to crystalline phases and influenced the type and amount of crystalline material formed. This research provides valuable insights for potential applications in the fields of nutraceutics and drug delivery.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Archaeology
Monica Tonelli, Rita Gelli, Rodorico Giorgi, Maria Isabella Pierige, Francesca Ridi, Piero Baglioni
Summary: The study developed three cement-based formulations to repair various types of damaged concrete-based monuments, including delamination issues and cracks of different sizes. By adding silica and halloysite nanotubes loaded with a corrosion inhibitor, the formulations showed high adhesion and corrosion resistance.
JOURNAL OF CULTURAL HERITAGE
(2021)
Article
Construction & Building Technology
Monica Tonelli, Rita Gelli, Francesca Ridi, Piero Baglioni
Summary: The study developed a novel material made of magnesium phosphate cement doped with Halloysite nanotubes for repairing cement-based structures, incorporating antimicrobial agents for anti-fouling protection. The addition of Halloysites not only enhanced the mechanical properties of the cement but also allowed for slow release of active molecules.
CONSTRUCTION AND BUILDING MATERIALS
(2021)
Article
Chemistry, Physical
Rita Gelli, Francesca Martini, Marco Geppi, Silvia Borsacchi, Francesca Ridi, Piero Baglioni
Summary: The study demonstrates that AMCPs can form in the presence of mucin, with the glycoprotein efficiently incorporated in the amorphous particles. Solid State NMR investigation reveals interactions between AMCPs and mucin, affecting the features of the glycoprotein.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Physical
Rita Gelli, Lorenzo Briccolani-Bandini, Marco Pagliai, Gianni Cardini, Francesca Ridi, Piero Baglioni
Summary: This study investigates the impact of Mg2+ on the structure and stability of Mg-substituted ACP nanoparticles by preparing particles with tunable composition and crystallinity. Results show that the stability of particles depends on the degree of Mg2+ substitution in the clusters, offering useful guidelines for the preparation and design of AMCP particles.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Rita Gelli, Giulia Mugnaini, Tessa Bolognesi, Massimo Bonini
Summary: Gelatin particles prepared by double emulsion methods exhibit excellent biocompatibility and versatility, with the ability to obtain porous microparticles with different structures. By adjusting preparation parameters, microparticles with tunable features can be designed.
Article
Chemistry, Physical
Rita Gelli, Valentina Pucci, Francesca Ridi, Piero Baglioni
Summary: This study describes the formation and crystallization mechanism of synthetic analogs of endogenous calciprotein particles (CPPs). Different stabilizing agents have different mechanisms of action in preventing the formation of CPPs or delaying their crystallization. These findings are of interest for developing effective therapies for vascular calcifications and deepening our understanding of amorphous calcium phosphate stabilization and its interaction with proteins.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Materials Science, Multidisciplinary
Rita Gelli, Lucrezia Sforzi, Francesco Montanari, Francesca Ridi, Piero Baglioni
Summary: Magnesium phosphate-based cements (MPCs) have been used as materials for bone repair. Gelatin microparticles were added as templating agents to create macroporous MPCs with the local release of alendronate, resulting in a material with ideal features for bone repair.
JOURNAL OF MATERIALS SCIENCE
(2022)
Article
Construction & Building Technology
Rita Gelli, Monica Tonelli, Francesca Martini, Lucia Calucci, Silvia Borsacchi, Francesca Ridi
Summary: This study explores the concentration dependent retarding effect of borax on tri-magnesium phosphate-based cements for the first time. By investigating the hydration and phases formed in the set cements using multiple characterization techniques, it was found that borax slows down the hydration process, reduces heat release, and leads to the formation of struvite instead of newberyite, without affecting the strength of the cement. The observed boron species suggest that the retardation is caused by the complexation of NH4+ and Mg2+ ions with borate and polyborate anions.
CONSTRUCTION AND BUILDING MATERIALS
(2022)
Article
Biophysics
Rita Gelli, Francesca Ridi
Summary: Calciprotein particles (CPPs) are colloidal hybrid particles formed in serum, consisting of amorphous calcium phosphate stabilized by fetuin-A protein. This study found that albumin, although abundant in serum, cannot prolong the lifetime of the amorphous phase of CPPs. However, albumin is effective in delaying the sedimentation of CPPs after crystallization and significantly decreases the amount and size of CPPs when present in their synthetic medium, playing a crucial role in the stabilization of CPPs.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Pharmacology & Pharmacy
Ivo Skorup, Gina Valentino, Simone Aleandri, Rita Gelli, Aymar Abel Ganguin, Eric Felli, Sonia Emilia Selicean, Rosanne Angela Marxer, Sarah Teworte, Ana Lucic, Jordi Gracia-Sancho, Annalisa Berzigotti, Francesca Ridi, Paola Luciani
Summary: Liver fibrosis is a disease characterized by the accumulation of extracellular matrix due to liver damage. Currently, there are no approved drugs specifically for treating liver fibrosis. Recent studies have shown that polyenylphosphatidylcholines (PPCs) can reverse fibrogenic features of hepatic stellate cells. In this study, a tablet formulation containing PPCs was developed and showed promising results in terms of its physical properties and bioactivity. The tablets, when taken orally, exerted the same antifibrotic effect as plain PPCs liposomes on fibrogenic cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Materials Science, Multidisciplinary
Giulia Mugnaini, Rita Gelli, Leonardo Mori, Massimo Bonini
Summary: Gelatin-based hydrogels are widely used in various fields due to their biocompatibility and versatility. However, their poor thermal stability limits their applications. In this study, we investigated the use of glutaraldehyde and glyceraldehyde as cross-linking agents in gelatin hydrogels. We found that by adjusting the type and amount of the cross-linker, we can tune the properties of the hydrogels, including their dissolution behavior, micro- and nanostructure, and mechanical properties. These findings are important for finding alternative cross-linking agents without toxicity concerns.
ACS APPLIED POLYMER MATERIALS
(2023)
Review
Engineering, Biomedical
Rita Gelli, Francesca Ridi
Summary: In the search for effective biomaterials for bone repair, magnesium phosphate cements (MPCs) are gaining importance as bone void fillers. This review highlights the main properties and applications of MPCs in the orthopedic field, discussing different formulations and their in vivo and in vitro response. The results showcase the potential of MPCs in orthopedics and provide strategies for further clinical application.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Materials Science, Biomaterials
Rita Gelli, Monica Tonelli, Francesca Ridi, Dominik Terefinko, Anna Dzimitrowicz, Pawel Pohl, Aleksandra Bielawska-Pohl, Piotr Jamroz, Aleksandra Klimczak, Massimo Bonini
Summary: Atmospheric pressure plasma treatments can modify the surface properties of magnesium phosphate-based cements (MPCs), resulting in changes in the relative amounts of different phases. These modified MPCs show good biocompatibility and promote cell proliferation, adhesion, and osteogenic differentiation.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
