Article
Chemistry, Physical
Satoshi Ishii, Shogo Yoshimoto, Katsutoshi Hori
Summary: The research showed that Acinetobacter sp. Tol 5 has strong adhesion force, mainly achieved through the interaction between the AtaA protein on the cell surface and the surface. This adhesion force increases with the increase of AtaA molecules. Amino acid hydrolysates and deionized water can inhibit the direct interaction between cells and surfaces, respectively.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Physical
Xinran Zhang, Jiangang Guo, Weigao Zhao
Summary: The adhesion force between a single phenyl ring and monolayer graphene was measured and calculated through experimental and theoretical methods. The single-bond force played a key role in the desorption process and can be divided into two stages.
APPLIED SURFACE SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Peter N. Lipke, Jason M. Rauceo, Albertus Viljoen
Summary: Mating and DNA transfer are crucial events for living organisms, and cell adhesion between gametes is essential for facilitating mating. Studies have provided insights into the structural information, specificities, and affinities of mating adhesins. Single-cell force spectroscopy has furthered our understanding of the design and evolution of these specialized adhesion proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Siying Li, Xuelei Pang, Jing Zhao, Qingrong Zhang, Yuping Shan
Summary: Targeted peptides are important in the construction of intelligent drug delivery systems, and the interaction between peptides and receptors is crucial for the efficacy of targeted nano-drugs. Using single-molecule force spectroscopy, this study evaluated the stability and effectiveness of three common peptides targeting specific receptors on living cell surfaces. The T7-TfR bond was found to be the most stable among the three peptide-receptor pairs.
Article
Multidisciplinary Sciences
Keunyeong Kim, Byungkook Jang, Sanghoon Lee, Gisu Park
Summary: This study compared three different force measurement techniques in a shock tunnel, showing similar drag coefficients but varying levels of measurement uncertainties.
Article
Multidisciplinary Sciences
Keunyeong Kim, Byungkook Jang, Sanghoon Lee, Gisu Park
Summary: Three force measurement techniques in a shock tunnel were evaluated and compared in this study. The system setup, data processing method, measurement uncertainties, and applied range of each technique were described and compared. Results showed that the three techniques yielded similar drag coefficients, but the order of increasing measurement uncertainties varied.
Review
Chemistry, Physical
Mi Li, Lianqing Liu, Tomaso Zambelli
Summary: Fluidic force microscopy (FluidFM), combining AFM with microchanneled cantilevers, allows for force-sensitive nanopipette manipulation under aqueous conditions. It has unique advantages in simultaneous three-dimensional manipulations and mechanical measurements at the micro-/nanoscale, showing great potential in biophysical assays, particularly at the single-cell level.
Article
Immunology
Xiaoxu Zhang, Bixia Hong, Peng Wei, Pengfei Pei, Haifeng Xu, Long Chen, Yigang Tong, Jialin Chen, Shi-Zhong Luo, Huahao Fan, Chengzhi He
Summary: This study investigated the adhesion of spike proteins from different variants to ACE2 using single-molecule and single-cell force spectroscopy. The results showed that the binding force was highest for the Delta variant, and slightly higher for the Omicron variant compared to the wild type.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Materials Science, Paper & Wood
Eko Setio Wibowo, Byung-Dae Park
Summary: This study measured the surface adhesion between cellulose and urea-formaldehyde resin adhesives. The results showed that the adhesion between 1.6 UF resins and nanocellulose is stronger due to the dominance of van der Waals forces. The higher surface free energy of 1.6 UF resins also contributes to the stronger adhesion.
Article
Multidisciplinary Sciences
Filippo Bonafe, Francesco Decataldo, Isabella Zironi, Daniel Remondini, Tobias Cramer, Beatrice Fraboni
Summary: Research on electrolyte-gated and organic electrochemical transistor (OECT) architectures aims to develop a highly biocompatible interface for amplifying bioelectronic signals at the site of detection. This study presents a model experiment to simulate the detection of a single cell using impedance sensing, and provides clear guidelines for optimizing OECTs as single cell sensors. The experimental results demonstrate significant gain compared to traditional electrode-based impedance sensors.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Electrical & Electronic
Yaowei Liu, Lu Li, Huiying Gong, Ke Li, Qili Zhao, Ruixin Li, Linkun Zhang, Xin Zhao, Mingzhu Sun
Summary: This study aimed to develop a robotic system for automated cell aspiration and in situ micropipette cleaning to measure cellular shear adhesion force. The system achieved automation through cell selection based on image texture analysis, contact detection based on motion history image analysis, and cell aspiration state detection based on sub-pixel image registration. Experimental results showed that pancreatic cancer cells possessed higher shear adhesion force than osteoblasts and osteoclasts, with a significant difference between osteoblasts and osteoclasts.
IEEE SENSORS JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Wenting Wang, Wenhao Zhang, Xiqi Wu, Kaidi Zhang, Yuhang Chen
Summary: The study investigates the interfacial properties between thin films and elastomers by analyzing the interface adhesion between a graphite nanoflake and an elastic polymer. The relationship among interface adhesion, elastic strain energy, and buckle surface area is established, providing a promising approach to characterize the interfacial properties of multilayer samples. Various methods are applied to introduce buckles, and the uncertainty in measuring the interface adhesion is discussed.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biotechnology & Applied Microbiology
Masayuki Wada, Toshiyuki Nomura
Summary: This study investigated the microbial coaggregation of yeast and LAB cells and found that specific interactions between mannose on the yeast surface and the mannose-binding protein (MBP) on the LAB surface were the main factors in forming small aggregates.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Review
Cell Biology
Ashwini Shinde, Kavitha Illath, Pallavi Gupta, Pallavi Shinde, Ki-Taek Lim, Moeto Nagai, Tuhin Subhra Santra
Summary: Cells exert, sense, and respond to physical forces through diverse mechanisms, translating them into biochemical signals. Cell adhesion plays a crucial role in development, maintaining tissue morphogenesis and activating critical signaling pathways. Understanding cell adhesion regulation during development and its contribution to diseases with quantitative methods is essential.
Article
Cell Biology
Agoston G. Nagy, Inna Szekacs, Attila Bonyar, Robert Horvath
Summary: This study used high-throughput fluidic force microscopy to investigate the cellular adhesion maturation in epithelial cells. The results showed a significant strengthening adhesive tendency during the formation of Vero monolayers, but this step-by-step maturation was absent in HeLa cells. Additionally, cancerous HeLa cells displayed stronger adhesion to the tight Vero monolayer than cells of the same origin, and the mechanical characteristics of Vero monolayers upon cancerous HeLa cell influence were recorded and analyzed.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Chemistry, Physical
Pal Jedlovszky, Reka A. Horvath, Milan Szori
JOURNAL OF PHYSICAL CHEMISTRY C
(2020)
Article
Chemistry, Physical
Hugh Shearer Lawson, Gabor Hollo, Robert Horvath, Hiroyuki Kitahata, Istvan Lagzi
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2020)
Article
Multidisciplinary Sciences
Nicolett Kanyo, Kinga Dora Kovacs, Andras Saftics, Inna Szekacs, Beatrix Peter, Ana R. Santa-Maria, Fruzsina R. Walter, Andras Der, Maria A. Deli, Robert Horvath
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Hajnalka Jankovics, Boglarka Kovacs, Andras Saftics, Tamas Gerecsei, Eva Toth, Inna Szekacs, Ferenc Vonderviszt, Robert Horvath
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Analytical
Tamas Gerecsei, Peter Chrenko, Nicolett Kanyo, Beatrix Peter, Attila Bonyar, Inna Szekacs, Balint Szabo, Robert Horvath
Summary: This study utilized a computer-controlled micropipette (CCMP) to measure the dissociation constant (K-d) of integrin-RGD binding, combined with techniques such as surface coating and local flow inducing hydrodynamic lifting force to study cell adhesion behavior. The results demonstrated that CCMP could effectively characterize cell adhesion at both single-cell and whole population levels, offering a convenient and automated approach for quantifying cell adhesivity before further processing.
Article
Biochemistry & Molecular Biology
Beatrix Peter, Imre Boldizsar, Gabor M. Kovacs, Anna Erdei, Zsuzsa Bajtay, Alexandra Voros, Jeremy J. Ramsden, Ildiko Szabo, Szilvia Bosze, Robert Horvath
Summary: Plants and fungi produce bioactive metabolites that can benefit human health, with anti-inflammatory and anticancer effects by influencing cellular adhesion and migration. By systematically analyzing the effects of natural metabolites on adhesion and movement, as well as their role in gene expression, new label-free methods can be applied for quicker and easier screening of natural compounds, allowing for greater quantification than before. Recent studies on natural extracts affecting SARS-CoV-2 treatments mainly focus on the adhesion and entry of the virus.
Article
Chemistry, Analytical
Eniko Farkas, Robert Tarr, Tamas Gerecsei, Andras Saftics, Kinga Dora Kovacs, Balazs Stercz, Judit Domokos, Beatrix Peter, Sandor Kurunczi, Inna Szekacs, Attila Bonyar, Anita Banyai, Peter Furjes, Szilvia Ruszkai-Szaniszlo, Mate Varga, Barnabas Szabo, Eszter Ostorhazi, Dora Szabo, Robert Horvath
Summary: There is a growing demand for bacteria repellent surfaces and antibody-based coatings in the field of biosensors. The optical waveguide lightmode spectroscopy (OWLS) technique offers a non-invasive and label-free method for characterizing protein and bacterial adsorption. In this study, an OWLS-based method was used to develop bacteria repellent surfaces and characterize different antibody-based coatings for bacterial assays. Nonspecific binding blocking agents were tested and immobilization methods for selected antibodies were applied. The performance of the biosensor was evaluated using enzyme-linked immunosorbent assay (ELISA) measurements. The best performance was achieved with a polyclonal antibody in combination with protein A-based immobilization and blocking of nonspecific binding.
Review
Chemistry, Analytical
Beatrix Peter, Eniko Farkas, Sandor Kurunczi, Zoltan Szittner, Szilvia Bosze, Jeremy J. Ramsden, Inna Szekacs, Robert Horvath
Summary: This article provides an overview and potential future directions of label-free biosensors in bacteria monitoring. While these sensors have the ability to detect bacteria adhesion, biofilm formation, and the effect of antibiotics, their application in basic biological research is still limited. The article discusses optical, mechanical, and electrical sensing technologies and summarizes key kinetic processes related to bacterial adhesion and survival, which could be explored further using label-free technologies. Additionally, the article explores the importance of antibacterial resistance, strategies to control adhesion, and bacteria-mammalian host cell interactions in the development and applications of biosensors.
Article
Biochemistry & Molecular Biology
Borbala Gemes, Eszter Takacs, Inna Szekacs, Robert Horvath, Andras Szekacs
Summary: The study demonstrates that glyphosate and its metabolites can inhibit multiple RGD binding integrins, affecting cell adhesion and physiological functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Anita Banyai, Eniko Farkas, Hajnalka Jankovics, Inna Szekacs, Eszter Leelossyne Toth, Ferenc Vonderviszt, Robert Horvath, Mate Varga, Peter Furjes
Summary: The recent work aims to provide a better understanding of how Dean vortices impact lateral focusing and investigate how cell morphology influences the focusing position compared to spherical particles. Different flow rates and bead diameters were used to examine the position and extent of the focused region. The study generated a precise map of equilibrium positions for spherical beads, serving as a benchmark for focusing multi-dimensional particles and cells. Biological samples of various shapes, including rod-shaped E. coli, discoid biconcave-shaped RBCs, round or ovoid-shaped yeast, and soft-irregular-shaped cancer cells, were studied to explore the relationship between cell shape and the focusing position at the channel's end.
Article
Materials Science, Multidisciplinary
Beatrix Peter, Nicolett Kanyo, Inna Szekacs, Antal Csampai, Szilvia Bosze, Robert Horvath
Summary: This study revealed the interaction between fibronectin and the natural active substance EGCG and its oxidized form, and investigated their effects on cancer cell adhesion. The results showed that both forms of polyphenols can bind to fibronectin and inhibit cellular adhesion, with the oxidized form having a more pronounced effect. The study also proposed a novel molecular scale active mechanism involving fibronectin's disulfide bridges to explain the observed kinetic signals and highlight the protein's role in adhesion.
MATERIALS ADVANCES
(2022)
Article
Chemistry, Physical
Tamas Gerecsei, Rita Ungai-Salanki, Andras Saftics, Imre Derenyi, Robert Horvath, Balint Szabo
Summary: This article describes the dissolution of water in oil, and derives simple equations to calculate the diffusion coefficient of water in oil. This is important for the production of miniature reactors for crystallization.
COLLOIDS AND INTERFACES
(2022)
Article
Chemistry, Physical
Kinga Dora Kovacs, Martin Novak, Zoltan Hajnal, Csaba Hos, Balint Szabo, Inna Szekacs, Ye Fang, Attila Bonyar, Robert Horvath
Summary: Fluidic flow is crucial in colloid and interface sciences, and monitoring cell response to varied flow velocities using optical biosensors presents a challenging yet innovative approach. By rotating liquid over a biosensor array, the study demonstrated the capability to measure cell adhesion and detachment at different flow velocities simultaneously, providing insights into cellular behavior under mechanical and chemical stimulation. This setup offers a new avenue for investigating interface phenomena under controlled flow conditions.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Physical
Reka A. Horvath, George Horvai, Abdenacer Idrissi, Pal Jedlovszky
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2020)
Article
Chemistry, Analytical
Beatrix Peter, Andras Saftics, Boglarka Kovacs, Sandor Kurunczi, Robert Horvath
