Article
Nanoscience & Nanotechnology
Shengxian Li, Si Dong, Jing Wu, Xinping Lv, Ning Yang, Qiuyu Wei, Chunxi Wang, Jingtao Chen
Summary: To improve the efficiency of cancer vaccines, personalized cancer vaccines and effective delivery systems are required. In this study, personalized cancer cell membranes (CCMs) were prepared from surgically harvested cancer tissues and used as tumor-associated antigens (TAAs). The personalized CCMs were then coated onto nanoparticles loaded with imiquimod (R837) to efficiently deliver them to draining lymph nodes and elicit enhanced antitumor immune responses. When combined with programmed cell death-1 antibodies, the coated nanoparticles exhibited high efficiency against tumor progression. Therefore, personalized CCM-coated nanoparticles represent a promising cancer vaccine with significant potential for cancer immunotherapy.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Immunology
Tanjila Hasan, Ryo Kawanishi, Hidetaka Akita, Yoshifumi Nishikawa
Summary: ssPalmE-LNP is an effective carrier for DNA vaccines against protozoan infections, leading to increased survival rates and antibody production in mice. Further immunological studies are needed to explore the mechanism of protective immunity induced by ssPalmE-LNPTgGRA15.
Review
Pharmacology & Pharmacy
Abdellatif Bouazzaoui, Ahmed A. H. Abdellatif, Faisal A. Al-Allaf, Neda M. Bogari, Saied Al-Dehlawi, Sameer H. Qari
Summary: The current COVID-19 pandemic has prompted a global effort to develop effective vaccines against the SARS-CoV-2 virus, which has caused significant economic, social, and psychological concerns. Research teams worldwide are adopting various strategies, from conventional approaches to novel methods, to combat this virulent pathogen and address the challenges in vaccine development.
Article
Materials Science, Multidisciplinary
Qiuyan Guo, Chufan Wang, Qiang Zhang, Keman Cheng, Wenjun Shan, Xiumin Wang, Jun Yang, Yunlong Wang, Lei Ren
Summary: A novel tumor vaccine delivery strategy using a biodegradable microneedle patch has been developed, which allows sustained release of tumor antigen and induces long term anti-tumor response. Incorporating mesoporous silica nanoparticles as a vaccine adjuvant enhances DC function and increases OVA presentation on DCs. This vaccine platform shows potential in tumor therapy with enhanced therapeutic effect and long term immune memory.
APPLIED MATERIALS TODAY
(2021)
Review
Immunology
Samantha J. Paston, Victoria A. Brentville, Peter Symonds, Lindy G. Durrant
Summary: Vaccination has had a significant impact in preventing infectious diseases, but the development of therapeutic cancer vaccines has been challenging.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Dengning Xia, Rui Jin, Gaurav Byagathvalli, Huan Yu, Ling Ye, Chao-Yi Lu, M. Saad Bhamla, Chinglai Yang, Mark R. Prausnitz
Summary: The novel ePatch system enables low-cost, handheld DNA vaccination against SARS-CoV-2, inducing strong antibody responses with at least 10-fold dose sparing compared to conventional injection methods. The vaccination process is well tolerated with mild, transient effects on the skin, offering a portable and accessible approach for combating COVID-19 and other epidemics.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Elana Ben-Akiva, Johan Karlsson, Shayan Hemmati, Hongzhe Yu, Stephany Y. Tzeng, Drew M. Pardoll, Jordan J. Green
Summary: Nanoparticle-based mRNA cancer vaccines show great promise for personalized cancer treatments. We developed a bioreducible lipophilic poly(beta-amino ester) nanocarrier that can efficiently deliver multiple antigen-encoding mRNAs and nucleic acid-based adjuvants to dendritic cells. The engineered nanocarrier facilitated targeted delivery without the need for surface functionalization and resulted in robust CD8+ T cell responses and efficient antitumor therapy in murine melanoma and colon adenocarcinoma models.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Materials Science, Multidisciplinary
Shengxian Li, Jing Wu, Xiaoping Li, Jingtao Chen, Chunxi Wang
Summary: Cancer vaccines are a promising strategy for preventing cancer progression through activating antitumor immunity. However, their clinical application is limited by systemic toxicity and low immunogenicity. To overcome these limitations, researchers are exploring the use of biomaterials as immune adjuvants in cancer vaccines to enhance immunogenicity and antigen presentation.
MATERIALS & DESIGN
(2022)
Review
Biochemistry & Molecular Biology
Sandeep Pallerla, Ata ur Rahman Mohammed Abdul, Jill Comeau, Seetharama Jois
Summary: Breast cancer is a major cause of death in women, with improvements in targeted therapies but still facing issues of resistance and limitations. Vaccine therapy introduces antigens to activate the immune system, providing longer-term immune memory to prevent cancer relapse.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Farrukh Jamil, Laiba Aslam, Laraib, Hira Ali, Kainaat Shoukat, Muhammad Asif Rasheed, Sohail Raza, Muhammad Ibrahim
Summary: A new multi-epitope vaccine with higher efficiency against Newcastle disease virus (NDV) has been designed by shuffling cytotoxic T lymphocytes segments. This new vaccine construct shows improved immunogenicity, antigenicity, and stability, making it more effective against NDV strains in Pakistan.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Marisa E. Aikins, You Qin, Hannah E. Dobson, Alireza Hassani Najafabadi, Kexing Lyu, Yao Xu, Ying Xin, Anna Schwendeman, Max S. Wicha, Alfred E. Chang, Qiao Li, James J. Moon
Summary: One major obstacle in cancer treatment is the presence of cancer stem cells (CSCs), which are resistant to traditional therapies and contribute to metastasis and relapse. In this study, a therapeutic vaccine targeting CSCs in melanoma was developed and shown to effectively reduce tumor growth and improve survival. The vaccine stimulated strong T cell responses against CSC-associated antigens and promoted infiltration of CD8+ T cells into tumors, while reducing the frequency of CSCs. This approach represents a safe and cost-effective strategy for targeting and eliminating CSCs in melanoma and other types of cancer.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Chemistry, Multidisciplinary
Zeinab Y. Al Subeh, Dakota B. Poschel, Priscilla S. Redd, John D. Klement, Alyssa D. Merting, Dafeng Yang, Megh Mehta, Huidong Shi, Yolonda L. Colson, Nicholas H. Oberlies, Cedric J. Pearce, Aaron H. Colby, Mark W. Grinstaff, Kebin Liu
Summary: Fas ligand (FasL) expressed on activated cytotoxic T lymphocytes (CTLs) binds to the cell surface receptor Fas, initiating a variety of signaling pathways and resulting in anti-tumor effects. Research has shown that Fas is crucial for the efficacy of CAR-T cell immunotherapy. Restoring Fas expression in tumor cells can overcome resistance to T cell cytotoxicity in human melanoma.
Review
Pharmacology & Pharmacy
Megan McNamee, Shuyi Wong, Owen Guy, Sanjiv Sharma
Summary: Microneedle fabrication was developed in the 1970s for painless transdermal drug delivery. In the past two decades, there has been significant research and investment in this area, with the emergence of SARS-CoV-2 and other vaccines accelerating their application for in vivo intradermal vaccine delivery. Microneedle arrays can be fabricated in different shapes, geometries, formats, and materials.
EXPERT OPINION ON DRUG DELIVERY
(2023)
Article
Engineering, Biomedical
Wenjing Li, Zhe Jing, Shuqing Wang, Qiyu Li, Yutong Xing, Haobo Shi, Shuang Li, Zhangyong Hong
Summary: This study utilized virus-like particles (VLPs) derived from the phage P22 to deliver peptide antigens efficiently for therapeutic cancer vaccines. Results demonstrated the potential advantages of VLPs as vaccine carriers and showed that VLP-OVAT significantly inhibited tumor growth in mouse models.
Article
Immunology
Clarety Kaseke, Rhoda Tano-Menka, Fernando Senjobe, Gaurav D. Gaiha
Summary: The development of an effective HIV cure is crucial, with the role of CTLs in suppressing viral outgrowth without therapy being emphasized. Successful containment of the virus in vivo is dependent on targeting mutation-resistant regions within the HIV proteome, highlighting the potential for therapeutic vaccine development.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Instruments & Instrumentation
Yu Wu, Lalitkumar K. Vora, Ryan F. Donnelly, Thakur Raghu Raj Singh
Summary: In this study, dissolving bilayer microneedles were developed as a safe and effective intraocular protein delivery system for the treatment of retinal vascular disorders.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Engineering, Biomedical
Adrian D. Juncos Bombin, Nicholas Dunne, Helen O. McCarthy
Summary: The downregulation of miR-31 and miR-132 is linked to delayed wound healing. In this study, it was hypothesized that delivering miR-31 and miR-132 intracellularly, either individually or in a blend, could enhance tissue repair. The use of a blend formulation can minimize toxicity and maximize therapeutic effects.
ACTA BIOMATERIALIA
(2023)
Review
Engineering, Biomedical
Achmad Himawan, Lalitkumar K. Vora, Andi Dian Permana, Sumarheni Sudir, Airin R. Nurdin, Ririn Nislawati, Rafikah Hasyim, Christopher J. Scott, Ryan F. Donnelly
Summary: Extracellular tissue fluids contain important biomarkers, but their accessibility has been a challenge in clinical diagnosis and monitoring. Microneedle technology provides a minimally invasive solution to collect tissue fluid samples and utilize them for disease diagnosis and monitoring.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Qonita Kurnia Anjani, Sara Demartis, Fabiana Volpe-Zanutto, Huanhuan Li, Akmal Hidayat Bin Sabri, Elisabetta Gavini, Ryan F. F. Donnelly
Summary: Rose Bengal (RB) is a fluorescent dye used in biomedical applications, especially in dermatology. Advanced delivery systems have been developed to enhance its permeation across the skin. However, there is currently no validated method for quantitatively analyzing RB in the skin. This study proposes fluorescence-based techniques and describes the development and validation of a fluorescence-coupled HPLC method to quantify RB in the skin matrix for the first time. Qualitative analysis using multiphoton microscopy and fluorescence emission spectra provides additional insight into RB's distribution and behavior in different environments.
Article
Pharmacology & Pharmacy
Delly Ramadon, Lissa Florencia Putri Sutrisna, Yahdiana Harahap, Kurnia Sari Setio Putri, Fathin Ulayya, Pietradewi Hartrianti, Qonita Kurnia Anjani, Ryan F. Donnelly
Summary: This study aimed to formulate and evaluate Lidocaine hydrochloride (LiH)-loaded dissolving microneedles (DMNs) with different polymer bases. Based on the in vitro permeation study, LiH-loaded DMNs with hyaluronic acid (10%) and poly(vinyl pyrrolidone) (25%) were found to deposit in the skin with safe concentrations of the drug permeating into systemic circulation.+/-
Review
Biotechnology & Applied Microbiology
Nikoletta Sargioti, Tanya J. J. Levingstone, Eoin D. D. O'Cearbhaill, Helen O. O. McCarthy, Nicholas J. J. Dunne
Summary: Current procedures for transdermal drug delivery have limitations in administering nucleic acid and small or large drug molecules, and causing pain and stress for needle phobic individuals. The development of a painless micro-sized device for efficient drug delivery is still needed. Recent advances in 3D printing have made it possible to fabricate metallic microneedle arrays, which have the potential to overcome the limitations of traditional systems. This review summarizes the types and modes of drug delivery of microneedles in transdermal drug delivery, discusses their applications in the treatment of diseases, and explores the fabrication techniques and geometrical characteristics of solid metallic microneedles.
BIOENGINEERING-BASEL
(2023)
Article
Pharmacology & Pharmacy
Marco T. A. Abbate, Inken K. Ramoller, Akmal H. Sabri, Alejandro J. Paredes, Aaron J. Hutton, Peter E. McKenna, Ke Peng, Jessica A. Hollett, Helen O. McCarthy, Ryan F. Donnelly
Summary: HIV/AIDS remains a global public health issue, which can lead to HIV-associated neurocognitive disorder due to the restriction of drugs crossing the blood-brain barrier. The nose-to-brain pathway and facial intradermal injection provide potential solutions to deliver drugs into the central nervous system. Nanoparticles and microneedle arrays are effective in increasing drug delivery via these routes.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Multidisciplinary
Yara A. Naser, Ismaiel A. Tekko, Lalitkumar K. Vora, Ke Peng, Qonita K. Anjani, Brett Greer, Christopher Elliott, Helen O. McCarthy, Ryan F. Donnelly
Summary: This study demonstrates the successful transdermal long-acting delivery of the hydrophobic drug atorvastatin (ATR) via hydrogel-forming microarray patches (HF-MAPs) using poly(ethylene)glycol (PEG)-based solid dispersion (SD) reservoirs.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Li Zhao, Lalitkumar K. Vora, Stephen A. Kelly, Linlin Li, Eneko Larraneta, Helen O. McCarthy, Ryan F. Donnelly
Summary: Antibiotic resistance is a serious health problem and is expected to worsen in the future. Researchers have developed a hydrogel-forming microarray patch system that can deliver antibiotics in a sustained manner.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Engineering, Biomedical
Sophie M. Coulter, Sreekanth Pentlavalli, Lalitkumar K. Vora, Yuming An, Emily R. Cross, Ke Peng, Kate McAulay, Ralf Schweins, Ryan F. Donnelly, Helen O. McCarthy, Garry Laverty
Summary: Eradicating HIV/AIDS by 2030 is a central goal of the World Health Organization. Patient adherence to complicated dosage regimens remains a key barrier. This paper presents an alternative platform, an injectable in situ forming hydrogel implant to deliver a model antiretroviral drug (zidovudine [AZT]) over 28 days.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Medicinal
Delly Ramadon, Fathin Ulayya, Annisa Sakinah Qur'ani, Iskandarsyah Iskandarsyah, Yahdiana Harahap, Qonita Kurnia Anjani, Vania Aileen, Pietradewi Hartrianti, Ryan F. F. Donnelly
Summary: This study aimed to develop dissolving microneedles (DMN) containing ketoprofen-loaded nanosuspension (NS) and co-grinding (CG) formulations. The most promising formulations for transdermal delivery of ketoprofen were F5-MN-NS (PVA 5%-PVP 10%) and F11-MN-CG (PVA 7.5%-PVP 15%). These findings suggest that the combination of DMN with nanosuspension or a co-grinding system can effectively enhance the transdermal delivery of ketoprofen.
Article
Chemistry, Multidisciplinary
Phillip Chambers, Monika Ziminska, Ahmed Elkashif, Jordan Wilson, John Redmond, Antzela Tzagiollari, Cole Ferreira, Auden Balouch, Jasmine Bogle, Seth W. Donahue, Nicholas J. Dunne, Helen O. Mccarthy
Summary: Bone-related injuries and diseases are a common cause of morbidity worldwide. This study demonstrates the potential of using microRNA (miRNA) gene therapy, specifically miR-26a, for bone healing. The researchers found that delivering miR-26a using the peptide RALA was effective in regulating osteogenic signaling in mesenchymal stem cells. They also showed that treating critical bone defects with RALA/miR-26a nanoparticles resulted in increased bone volume, density, and blood vessel formation. These findings highlight the utility of miRNA gene therapy in promoting bone healing, calling for further investigation in in vivo models.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Cian M. Mccrudden, Lindsey Bennie, Philip Chambers, Jordan Wilson, Megan Kerr, Monika Ziminska, Hayley Douglas, Sarah Kuhn, Emma Carroll, Garrett O'Brien, Niamh Buckley, Nicholas J. Dunne, Helen O. Mccarthy
Summary: Lipid nanoparticles (LNP) have played a crucial role in the success of mRNA vaccines and have opened up new opportunities for therapeutics. This study investigated the use of RALA-SME nanoparticles, which contained a blend of Spike, Membrane, and Envelope antigens, for immunization. The results showed that RALA-SME induced a robust immune response, with highly significant antigen-specific antibodies and T-cell response. Immunization with RALA-SME derivatives in Syrian Golden hamsters resulted in the production of functional antibodies capable of neutralizing SARS-CoV-2 from different lineages, providing protection against disease.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Materials Science, Biomaterials
Juhaina M. Abu Ershaid, Lalitkumar K. Vora, Fabiana Volpe-Zanutto, Akmal H. Sabri, Ke Peng, Qonita K. Anjani, Peter E. Mckenna, Anastasia Ripolin, Eneko Larraneta, Helen O. Mccarthy, Ryan F. Donnelly
Summary: Schizophrenia is a severe mental illness characterized by impaired emotion and cognition. Injectable antipsychotics, although designed to enhance adherence, are invasive and require professional administration. To overcome these issues, research has focused on developing transdermal antipsychotic formulations.
BIOMATERIALS ADVANCES
(2023)
Article
Instruments & Instrumentation
Ryan F. Donnelly, Mark R. Prausnitz
Summary: Microneedle (MN) technologies offer the opportunity to improve drug delivery and treatment by targeting specific tissues. Hollow MNs have been FDA-approved for targeted drug delivery to skin-resident immune cells and specific parts of the eye. MN technology also has applications in cosmetic products. With further research and approved products, MN technology shows promising growth potential.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
