Article
Medicine, General & Internal
Vanessa Vedder, Verena Schildgen, Jessica Lusebrink, Ramona Liza Tillmann, Birgitta Domscheit, Wolfram Windisch, Christian Karagiannidis, Michael Brockmann, Oliver Schildgen
Summary: The study compared the cytological profiles of BALs from patients infected with various respiratory pathogens, finding that different pathogens caused distinct cell pattern changes but no general cell distribution pattern. Although the cytology pattern of BAL fluid is not specific enough to be used as the sole diagnostic criterion for COVID-19, it may support clinical decision-making.
Review
Medicine, General & Internal
Alexandros Kalkanis, Dimitrios Papadopoulos, Dries Testelmans, Alexandra Kopitopoulou, Eva Boeykens, Els Wauters
Summary: Detecting molecular markers in bronchoscopy samples, specifically BALF, can enhance lung cancer diagnosis efficiency and evaluate tumor progression microenvironment. BALF analysis enables the detection of predictive and prognostic biomarkers and guides treatment choices and evaluation of therapy-induced toxicities.
Article
Multidisciplinary Sciences
Meijuan Song, Xiuwei Zhang, Yizhou Gao, Bing Wan, Jinqiang Wang, Jinghang Li, Yuanyuan Song, Xiaowei Shen, Li Wang, Mao Huang, Xiaowei Wang
Summary: By analyzing the lncRNAs in BALF exosomes, potential diagnostic and therapeutic targets for acute lung injury (ALI) can be identified.
Review
Biochemistry & Molecular Biology
Lukasz Zareba, Jacek Szymanski, Zuzanna Homoncik, Malgorzata Czystowska-Kuzmicz
Summary: Extracellular vesicles (EVs) play a significant role in lung inflammation by transferring molecular cargo in BALF, influencing the alveolar microenvironment. The information transferred by EVs can induce and propagate lung diseases, especially lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Wenwen Lin, Feng Huang, Yin Yuan, Qiaowei Li, Zhong Lin, Wenqing Zhu, Binbin Lin, Pengli Zhu
Summary: This study aims to investigate the effect of inflamed endothelial exosomes (exo-T) on macrophage function. The results showed that exposure to exo-T for 24 hours skewed macrophages towards the M1 subtype, exacerbated lipid deposition, and promoted macrophage apoptosis. However, it did not significantly affect macrophage migration. Furthermore, the study identified differentially expressed microRNAs (DE-miRNAs) in exo/exo-T and demonstrated that exo-T intervention improves the phosphorylation of MAPK/NF-κB-related proteins.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Pharmacology & Pharmacy
Yu Hua, Yan Ding, Yapeng Hou, Yanhong Liu, Kejun Mao, Yong Cui, Hongguang Nie
Summary: Exosomes play a crucial role in intercellular communication by delivering cargo to pulmonary target cells, contributing to the pathogenesis, diagnosis, and therapy of lung diseases.
CURRENT PHARMACEUTICAL DESIGN
(2021)
Article
Oncology
Pascal Bezel, Alan Valaperti, Urs Steiner, Dieter Scholtze, Stephan Wieser, Maya Vonow-Eisenring, Andrea Widmer, Benedikt Kowalski, Malcolm Kohler, Daniel P. Franzen
Summary: Our study analyzed cytokines in bronchoalveolar lavage fluid (BALF) of lung cancer patients, finding significant differences compared to healthy individuals, but these differences were not statistically significant when compared to other lung diseases in multivariate analysis. Cytokine levels in BALF were close to the lower detection limit and showed minimal correlation with their levels in serum of the same individual.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2021)
Letter
Respiratory System
S. D. Pouwels, Janette K. Burgess, Erik Verschuuren, Dirk-Jan Slebos
Summary: The study assessed the cellular composition of lung lining fluid at different locations within the lung post-lung transplantation. Neutrophils and lymphocytes decrease in more distal regions, while macrophages increase. This information can help determine the best sampling technique and location for measuring specific factors and biomarkers in lung lining fluid.
RESPIRATORY RESEARCH
(2021)
Review
Biology
Mariaenrica Tine, Davide Biondini, Marco Damin, Umberto Semenzato, Erica Bazzan, Graziella Turato
Summary: Lung cancer is the leading cause of cancer death worldwide, mainly due to delayed diagnosis in advanced stages. Recent advancements in targeted therapies and immunotherapies have emerged for specific genetic or immunological lung cancer patients. The identification and characterization of tumors rely on bioptic samples, but less invasive procedures are becoming more common. Extracellular vesicles (EV), released by various cells, have shown potential as diagnostic markers. This review summarizes the promising findings on lung-cancer-related EV, discussing their role as potential biomarkers and their impact on lung cancer pathogenesis.
Article
Medicine, General & Internal
Feng Zhu, Qinfang Ou, Jian Zheng, Min Zhou, Huaxin Chen, Xiufeng Jiang
Summary: The study evaluated the value of IL-27 in the diagnosis of smear-negative pulmonary TB, finding that BALF IL-27 had a higher diagnostic value for TB, especially in patients with negative smear results. Serum IL-27 could also serve as an auxiliary method for TB screening.
Article
Chemistry, Analytical
Linxiu Peng, Weichen Xu, Jingying Wang, Yan Liu, Wenjuan Qian, Shaodong Wang, Tong Xie, Jinjun Shan
Summary: Bronchoalveolar lavage (BAL) is a minimally invasive and well-tolerated procedure widely used for diagnosing pulmonary diseases. Lipid analysis of BAL fluid provides comprehensive information about lipid phenotypes, potential biomarkers, and biological mechanisms of respiratory diseases. However, the diverse concentration of lipids in BAL fluid poses a challenge in obtaining high-quality lipidomic data. This study investigated the optimal concentration multiple of BAL fluid for lipidomic analysis and found that freeze-drying/ reconstitution prior to lipid extraction increased the richness and abundance of lipids. The altered phospholipids in the BAL fluid of H1N1 infected mice were found, indicating disrupted inflammatory homeostasis in the lungs.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Pediatrics
Chiara Tirone, Federica Iavarone, Milena Tana, Alessandra Lio, Claudia Aurilia, Simonetta Costa, Massimo Castagnola, Irene Messana, Giovanni Vento
Summary: The study investigates the role of oxidative and proteolytic inactivation of alpha-1 antitrypsin (AAT) in the pathogenesis of bronchopulmonary dysplasia (BPD) in premature infants. Higher levels of AAT fragments and oxidized AAT fragments were found in preterm infants developing BPD, highlighting the central role of an imbalance between proteases and protease inhibitors in exacerbating lung injury and inducing severe forms of BPD.
FRONTIERS IN PEDIATRICS
(2021)
Article
Multidisciplinary Sciences
Nathan D. Maulding, Spencer Seiler, Alexander Pearson, Nicholas Kreusser, Joshua M. Stuart
Summary: The SARS-CoV-2 pandemic has posed challenges in understanding the health effects caused by the novel infection. Transcriptomic RNA sequencing has provided new insights into viral mechanisms and host responses. The study reveals tissue specificity of SARS-CoV-2 expression, inferred lipopolysaccharide response, and co-regulation with CXCL, SPRR, and S100. These findings have implications for future therapeutic research.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Xiaohua Liu, Yuxiang Du, Zhonghan Zhao, Jun Zou, Xiaojing Zhang, Lingli Zhang
Summary: White adipose tissue (WAT) functions as both an energy storage reservoir and a metabolically active endocrine organ. It can secrete various adipocytokines and synthesize and secrete exosomes, which play important roles in intercellular communication and physiological processes. The skeleton, on the other hand, acts as a protective organ, gives the body its shape, and is regulated by cytokines secreted by WAT. The link between WAT and bone lipid regulation has been identified, suggesting potential targets for treating skeletal diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Oncology
Lei Li, Zhujia Ye, Sai Yang, Hao Yang, Jing Jin, Yingying Zhu, Jinsheng Tao, Siyu Chen, Jiehan Xu, Yanying Liu, Weihe Liang, Bo Wang, Mengzhu Yang, Qiaoyun Huang, Zhiwei Chen, Weimin Li, Jian-Bing Fan, Dan Liu
Summary: A 5-marker model was developed to differentiate pulmonary adenocarcinoma and squamous carcinoma from benign diseases, especially for infection, inflammation, and tuberculosis, with high diagnostic accuracy. The model's performance is not affected by gender, age, smoking history, or the solid components of nodules.
CLINICAL EPIGENETICS
(2021)
Article
Virology
Jian Wu, Mariza Bortolanza, Guanghua Zhai, Anquan Shang, Zongxin Ling, Bin Jiang, Xiaochen Shen, Yiwen Yao, Jiong Yu, Lanjuan Li, Hongcui Cao
Summary: The study found no significant difference in bacterial diversity between patients with acute hepatitis E and healthy controls, but Proteobacteria, Gammaproteobacteria, and Enterobacteriaceae were more abundant in AHE patients. Functional prediction analysis revealed that ribosome, purine metabolism, and two-component system were the top three pathways affected by HEV infection. Furthermore, higher levels of interferon-gamma were associated with increased abundance of certain bacterial taxa in AHE patients, and Gammaproteobacteria abundance was positively correlated with liver enzyme levels.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Immunology
Chen Chen, Anquan Shang, Zujun Sun, Yuting Gao, Jingjuan Huang, Yili Ping, Wenjing Chang, Chenzheng Gu, Junjun Sun, Ping Ji, Yi Yuan, Renquan Lu, Dong Li
Summary: TERC is significantly upregulated in urinary exosomes from bladder urothelial carcinoma (BLCA) patients. Urinary exosomal TERC has higher sensitivity and accuracy, and could serve as a diagnostic and prognostic biomarker for BLCA.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Lipei Wu, Weiwei Wang, Jiale Tian, Chunrun Qi, Zhengxin Cai, Wenhui Yan, Shihai Xuan, Anquan Shang
Summary: Combination therapy of Nab-paclitaxel and IL-15 fusion protein effectively inhibits tumor growth in colorectal cancer and stimulates the activity of immune effector cells, thus suppressing the accumulation of immunosuppressive cells.
Article
Medicine, General & Internal
An Shang, Min Wang, Yongping Yang, Zeyun Zhao, Donglin Li, Yu Guo, Rui Qi, Yang Yang, Shuang Wang
Summary: In this study, TPDLP was found to have a favorable prognosis for perineal wounds with acceptable side-effects.
Article
Immunology
Jiale Tian, Weiwei Wang, Jichao Zhu, Yun Zhuang, Chunrun Qi, Zhengxin Cai, Wenhui Yan, Wenying Lu, Anquan Shang
Summary: In colorectal cancer, SETDB1 activates the BATF3/PD-L1 axis by inhibiting FOSB-mediated miR-22, promoting immune evasion in tumor.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Wenhui Yan, Wuchao Liu, Junlu Wu, Lipei Wu, Shihai Xuan, Weiwei Wang, Anquan Shang
Summary: This study found that NPY interacts with NPY2R in the amygdala to activate the MAPK signaling pathway, thereby promoting the occurrence of neuropathic pain.
Article
Oncology
Dan Qiao, Chenzheng Gu, Weiwei Wang, Wenhui Yan, Chenfei Jiang, Jingwen Hu, Anquan Shang, Jian Guo
Summary: This study identified tumor-originated exosomal hsa-miR-3937 as a potential and effective liquid biopsy marker for colorectal cancer detection and therapy, and investigated its role and mechanism in CRC.
JOURNAL OF ONCOLOGY
(2022)
Article
Medicine, General & Internal
Yu Guo, An Shang, Shuang Wang, Min Wang
Summary: The study found that CHMPs are significantly elevated in liver hepatocellular carcinoma and strongly associated with immune-cell infiltration, poor prognosis, multiple key pathways, and drug resistance.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2022)
Review
Immunology
Yuting Gao, Hao Zhou, Gege Liu, Junlu Wu, Yi Yuan, Anquan Shang
Summary: Lactic acid plays an important role in tumor development and its accumulation is detrimental to health. It acts as an immunomodulatory chemical and affects immune cell functions. Inhibiting lactate metabolism to enhance anticancer immune responses may alter lactate levels in the tumor microenvironment. Targeting lactate synthesis and sources has emerged as a potential therapeutic option for cancer treatment.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Article
Immunology
Chenzheng Gu, Junlu Wu, Wei Zhang, Yiwen Yao, Wenhui Yan, Yi Yuan, Weiwei Wang, Anquan Shang
Summary: This study investigated m6A methylation in ulcerative colitis (UC) and identified 9 potential m6A methylation-dependent gene regulatory networks. These findings contribute to the assessment of UC severity and the development of targeted therapeutic approaches for this disease.
JOURNAL OF IMMUNOLOGY RESEARCH
(2022)
Article
Oncology
Chenzheng Gu, Wenjing Chang, Junlu Wu, Yiwen Yao, Gege Liu, Yi Yuan, Wenqiang Quan, Zujun Sun, Anquan Shang, Dong Li
Summary: This study explored the expression patterns and prognostic relevance of NCOA4 in malignancies, and investigated its association with clinicopathological features and immune cell infiltration. The downregulation of NCOA4 was found to be associated with poor prognosis, particularly in colon adenocarcinoma.
JOURNAL OF ONCOLOGY
(2022)
Article
Oncology
Chenzheng Gu, Caiyun Li, Weiwei Wang, Wenhui Yan, Yiwen Yao, Meng Shi, Fei Han, Anquan Shang
Summary: Acute lung injury (ALI) is a severe form of sepsis with high morbidity and mortality. Epigenetic alterations play an important role in the pathophysiology of ALI, and using epigenetic inhibitors may reduce symptoms. Methylation of m6A is relevant in embryonic development, circadian rhythm, cell cycle, and alveolar lymphoma. Our study identified potential prognostic markers and therapeutic targets for ALI therapy using m6A and immune invading cells.
JOURNAL OF ONCOLOGY
(2022)
Article
Oncology
Weiwei Wang, Lipei Wu, Jiale Tian, Wenhui Yan, Chunrun Qi, Wuchao Liu, Shihai Xuan, Anquan Shang
Summary: This study investigated the role of EVs secreted by cervical cancer cells in cervical cancer. miR-146a-5p promotes the metastatic potential of cervical cancer cells by activating the YAP pathway to inhibit cofilin phosphorylation and promote the depolymerization of F-actin. EVs secreted by highly metastatic cervical cancer cells also promote the metastasis of low-metastatic cervical cancer cells.
JOURNAL OF ONCOLOGY
(2022)
Review
Oncology
Haiyang Wang, Jiayi Hu, Junlu Wu, Ping Ji, Anquan Shang, Dong Li
Summary: This article provides detailed information on the role of the microbiome in the occurrence and progression of lung cancer. It discusses mechanisms such as regulation of host immune activity, alteration of host metabolism, and dysbiosis of the microbiome. Researches have shown that the commensal microbiota plays a vital role in both tumor development and response to lung cancer treatment.
Article
Medical Laboratory Technology
Chenzheng Gu, Anquan Shang, Gege Liu, Jichao Zhu, Wei Zhang, Limin Jin, Zujun Sun, Dong Li
Summary: This study identified increased CD147 expression in exosomes as a potential diagnostic and prognostic biomarker for colorectal cancer (CRC). A high-sensitive exosome-counting system using specific antibodies was used for rapid and sensitive collection and measurement of exosomes.
CLINICA CHIMICA ACTA
(2023)
