Review
Biochemistry & Molecular Biology
Ivan Y. Y. Filin, Yuri P. P. Mayasin, Chulpan B. B. Kharisova, Anna V. Gorodilova, Kristina V. V. Kitaeva, Daria S. S. Chulpanova, Valeriya V. V. Solovyeva, Albert A. A. Rizvanov
Summary: Melanoma is a highly aggressive and therapy-resistant cancer, with increasing incidence rates. Conventional methods of treatment often result in relapse. Adjuvant therapies, such as cell-based immunotherapy, can be used to reduce recurrence risk by mobilizing the immune system to kill cancer cells. This review discusses the advantages, challenges, and ongoing clinical trials of cell-based therapy for adjuvant treatment of melanoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cardiac & Cardiovascular Systems
Mona Fiuzat, Carine E. Hamo, Javed Butler, William T. Abraham, Ersilia M. DeFilippis, Gregg C. Fonarow, Joann Lindenfeld, Robert J. Mentz, Mitchell A. Psotka, Scott D. Solomon, John R. Teerlink, Muthiah Vaduganathan, Orly Vardeny, John J. McMurray, Christopher M. O'Connor
Summary: This paper summarizes the discussion on the standardization of background drug therapy in heart failure treatment and provides potential conceptual approaches, with a focus on therapies indicated for HF with reduced ejection fraction.
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
(2022)
Review
Oncology
Hanna H. Kakish, Fasih Ali Ahmed, Mohamedraed Elshami, Alexander W. Loftus, Richard S. Hoehn, John B. Ammori, Lee M. Ocuin, Jordan M. Winter, Jeremy S. Bordeaux, Ankit Mangla, Luke D. Rothermel
Summary: In recent years, there has been an increasing focus on clinical trial investment in melanoma therapy, particularly in exploring novel drug mechanisms like immunotherapy and vaccines. Results indicate that ongoing trials may offer new treatment strategies for melanoma patients.
Review
Chemistry, Multidisciplinary
Junying Zhang, Hailiang Li, Lamyaa Albakr, Yiwen Zhang, Aiyu Lu, Wenlin Chen, Tianyu Shao, Luying Zhu, Hua Yuan, Gongjun Yang, Nial J. Wheate, Lifeng Kang, Chungyong Wu
Summary: This review provides an update and analysis on the latest progress of MN-based clinical trials since 2007. MNs have received extensive attention and have been widely used in local and systemic therapy due to their self-application, painlessness, and ability to carry multiple therapeutic agents. The different types of MNs in the trials, including hollow MNs, MN patches, radiofrequency MNs, MN rollers, and other MNs, are summarized along with their clinical trial time and regions. This review aims to present an overview of MN development and offer insights for future research, and it is the first review focused on MN clinical trials showcasing the latest applications of this advanced technology in medicine.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Nanoscience & Nanotechnology
Zhaosong Feng, Yuyang Gu, Mengping Yuan, Renzhong Xiao, Zhenghua Fei
Summary: This study aimed to synthesize current findings from clinical trials of liposomes in children's anticancer therapy (LCAT) listed on ClinicalTrials.gov. The results showed that most of the trials were non-randomized, single group assignment, and non-blinded interventional trials of small scale, focusing on organic chemicals, with leukemia, lymphoma, and ovarian cancer being the main diseases studied.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Oncology
Vivian Chua, Jane Mattei, Anna Han, Lauren Johnston, Kyleigh LiPira, Sara M. Selig, Richard D. Carvajal, Andrew E. Aplin, Sapna P. Patel
Summary: Uveal melanoma, a rare cancer in adults, poses a challenging treatment due to lack of effective options. However, ongoing clinical trials and novel targets show promise in improving treatment outcomes, highlighting the high interest and unmet needs in this field.
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Melissa M. de Meza, Willeke A. M. Blokx, Han J. Bonenkamp, Cristian U. Blank, Maureen J. B. Aarts, Franchette W. P. J. van den Berkmortel, Marye J. Boers-Sonderen, Jan Willem B. de Groot, John B. Haanen, Geke A. P. Hospers, Ellen W. Kapiteijn, Olivier J. van Not, Djura Piersma, Rozemarijn S. van Rijn, Marion A. Stevense-Den A. Boer, Astrid A. M. van Der Veldt, Gerard Vreugdenhil, Alfons J. M. van den Eertwegh, Karijn P. M. Suijkerbuijk, Michel W. J. M. Wouters
Summary: This study examines the adjuvant systemic therapy of in-transit melanoma (ITM) patients and compares the outcomes of different patient groups. The results indicate that ITM-only patients have similar recurrence-free survival and overall survival rates to non-ITM patients, while patients with both ITM and nodal disease have lower rates.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Review
Veterinary Sciences
Jessica A. Bertout, Philippe J. R. Baneux, Carol K. Robertson-Plouch
Summary: Ethical review is crucial for ensuring the welfare and safety of research subjects and data integrity. Differences exist between laws and standards for human and animal studies, with clinical research for client-owned animals having less defined regulations. Expanding the functions of veterinary clinical studies committees is proposed as a solution to address gaps in ethical review of clinical animal research in the United States.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Medicine, General & Internal
Simone Amabile, Gabriele Roccuzzo, Valentina Pala, Luca Tonella, Marco Rubatto, Martina Merli, Paolo Fava, Simone Ribero, Maria Teresa Fierro, Paola Queirolo, Pietro Quaglino
Summary: This narrative review focuses on adjuvant studies for resected stage III/IV melanoma, specifically looking at distant metastasis-free survival (DMFS) trends and their correlation with relapse-free survival (RFS). The data shows that DMFS trends closely follow those of RFS, with most patients experiencing relapse at visceral sites rather than regional sites. DMFS could be considered a primary endpoint in adjuvant trials, identifying patients with a worse prognosis who may need further systemic treatment.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Cell Biology
Jafar Rezaie, Maryam Feghhi, Tahereh Etemadi
Summary: Exosomes, as significant mediators of cell-to-cell communication, have promising applications in the treatment of various diseases. They can be used as biomarkers, cell-free therapeutic agents, and drug delivery carriers. However, there are challenges to overcome in the clinical application of exosomes, and further research and standardization are needed.
CELL COMMUNICATION AND SIGNALING
(2022)
Review
Pharmacology & Pharmacy
Carolina Iglesias-Lopez, Antonia Agusti, Antonio Vallano, Merce Obach
Summary: The study analyzed the methodological features of clinical trials supporting the marketing authorization of approved ATMPs in the EU, finding that the majority of trials were non-controlled, single-arm studies. A shift towards more robust clinical trial designs is expected in the future to better define the benefits and therapeutic added value of ATMPs.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Gastroenterology & Hepatology
Elliot B. Tapper, Marina Serper, David S. Goldberg
Summary: Patients with chronic liver disease can benefit from pragmatic trial designs, which aim to provide evidence for the adoption of interventions in real-world clinical practice. The efficiency of identifying, recruiting, and following patients, the resemblance of interventions and design to usual clinical care, and the importance of outcomes to patients are key factors in determining the pragmatism of a trial. This review focuses on the promise, trade-offs, and purpose of pragmatic trials in hepatology.
Review
Immunology
Mario Sousa-Pimenta, Angelo Martins, Jose Mario Mariz, Pedro Berraondo
Summary: This meta-analysis examined the efficacy of different therapeutic approaches in Richter syndrome through early clinical trials. The results showed that immunochemotherapy and combinatorial regimens were more effective, while cell-based therapies showed lower efficacy. Bispecific antibodies demonstrated promising response rates, while Bruton tyrosine kinase inhibitors had lower rates of complete response.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Oncology
Russell G. Witt, Derek J. Erstad, Jennifer A. Wargo
Summary: The treatment of malignant melanoma has undergone significant changes in recent years, including the use of immune checkpoint blockade, targeted therapy, and intralesional therapy. Despite improvements in patient response rates, there remains a subset of high-risk patients with surgically resectable disease. Neoadjuvant therapy offers several advantages for this high-risk group, including a stronger immune response, the ability to assess treatment response, and increased disease resectability.
THERAPEUTIC ADVANCES IN MEDICAL ONCOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Claus Garbe, Reinhard Dummer, Teresa Amaral, Rodabe N. Amaria, Paolo A. Ascierto, Elizabeth M. Burton, Brigitte Dreno, Alexander M. M. Eggermont, Axel Hauschild, Christoph Hoeller, Roland Kaufmann, Celeste Lebbe, Mario Mandala, Alexander M. Menzies, David Moreno, Olivier Michielin, Paul Nathan, Sapna P. Patel, Caroline Robert, Dirk Schadendorf, Paul C. Lorigan, Richard A. Scolyer, Hussein A. Tawbi, Bart A. van de Wiel, Christian Blank, Georgina V. Long
Summary: Individuals with cutaneous melanoma who receive neoadjuvant immunotherapy demonstrate significant improvement, which should be included in standard care.
Article
Cardiac & Cardiovascular Systems
Marek T. Tomala, Aleksander Trabka-Zawicki, Andrzej Machnik, Bartlomiej A. Nawrotek, Wojciech Zajdel, Ewa L. Stepien, Jacek Legutko, Krzysztof Zmudka
Summary: This study evaluated the efficacy of ticagrelor in comatose survivors of out-of-hospital cardiac arrest treated with therapeutic hypothermia. The results showed that therapeutic hypothermia did not affect the platelet-inhibitory effect of ticagrelor and did not increase the risk of stent thrombosis.
CARDIOLOGY JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Agnieszka Kaminska, Maciej Roman, Andrzej Wrobel, Agnieszka Gala-Bladzinska, Maciej T. Malecki, Czeslawa Paluszkiewicz, Ewa L. Stepien
Summary: This study verified the hypothesis that the Raman signature of urinary extracellular vesicles (UEVs) can be used to classify patients with diabetes at different stages of chronic kidney disease (CKD). The study found significant correlations between Raman bands measured for UEVs and clinical parameters, and significant differences in specific bands mainly derived from proteins and lipids between different study groups. Regression models including tryptophan and amide III bands were able to estimate the values of estimated glomerular filtration rate (eGFR).
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Thais S. M. Lima, Wanderson Souza, Luths R. O. Geaquinto, Priscila L. Sanches, Ewa L. Stepien, Joao Meneses, Eli Fernandez-de Gortari, Nicole Meisner-Kober, Martin Himly, Jose M. Granjeiro, Ana R. Ribeiro
Summary: The increasing use of nanomaterials has raised concerns about nanosafety and their potential toxic effects on human health. Studies have shown that nanomaterials can alter cell communication by reshaping and altering the secretion of extracellular vesicles (EVs), leading to dysfunction in recipient cells. However, our understanding of how the physicochemical characteristics of nanomaterials affect EV content and their physiological functions is limited. This review explores the relevance of EVs in the field of nanotoxicology and assesses the modulation of EVs by nanomaterial exposure and the regulation of signaling pathways and physiological responses.
Article
Cell Biology
Magdalena Surman, Sylwia Kedracka-Krok, Magdalena Wilczak, Piotr Rybczynski, Urszula Jankowska, Malgorzata Przybylo
Summary: Proteins carried by tumor-derived vesicles play an important role in cancer progression and have potential as diagnostic markers. This study compared protein content between vesicles released by normal and cancerous thyroid cells, and investigated the effects of these vesicles on other cells. The results showed that cancer-derived vesicles contained more proteins, some of which were unique and may be useful for thyroid cancer diagnosis and treatment. Additionally, these vesicles were found to promote cell proliferation and migration.
Article
Biochemistry & Molecular Biology
Magdalena E. Marzec, Carina Rzaca, Pawel Moskal, Ewa L. Stepien
Summary: Time-of-flight secondary ion mass spectrometry (TOF-SIMS) with the Bi3thorn liquid metal ion gun was used to investigate the content of lipids and amino acids in extracellular vesicles. The study confirmed the detrimental effect of hyperglycemia on lipids and amino acids in released extracellular vesicles.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Cell Biology
Anna Drozdz, Tomasz Kolodziej, Sonia Wrobel, Krzysztof Misztal, Marta Targosz-Korecka, Marek Drab, Robert Jach, Carina Rzaca, Magdalena Surman, Malgorzata Przybylo, Zenon Rajfur, Ewa L. Stepien
Summary: Extracellular vesicles, particularly LEVs, are important in intercellular communication. This study investigates the changes in human umbilical vein endothelial cells (HUVECs) under high glucose conditions (HGC) and the effects of LEVs released by endothelial cells on HUVECs. The findings reveal the impact of HGC on HUVEC shape, mobility, and metabolic pathways, and the ability of LEVs to activate gene expression related to protein synthesis and vesicle trafficking in HUVECs.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Magdalena Wilczak, Magdalena Surman, Malgorzata Przybylo
Summary: Bladder cancer is the 10th most common malignancy worldwide, with a high mortality rate. Altered glycosylation of bladder cancer cells plays a crucial role in disease progression and could be used for diagnostic and therapeutic purposes.
Article
Chemistry, Multidisciplinary
Barbara Zapala, Agnieszka Kaminska, Monika Piwowar, Agnieszka Paziewska, Agnieszka Gala-Bladzinska, Ewa L. Stepien
Summary: This study investigated the role of urine-derived extracellular vesicles (uEVs) in diabetic kidney disease (DKD) in patients with type 2 diabetes mellitus (T2DM), identifying specific miRNAs that are enriched or downregulated in T2DM patients with DKD. These miRNAs are involved in cellular processes such as apoptosis, inflammation, and tissue remodeling, and further experimental studies are needed to confirm these findings.
PHARMACEUTICAL RESEARCH
(2023)
Review
Physics, Multidisciplinary
Steven D. Bass, Sebastiano Mariazzi, Pawel Moskal, Ewa Stepien
Summary: Positronium, composed of an electron and a positron, reveals valuable insights into quantum electrodynamics (QED) and the structure and processes of biological materials at the nanoscale. Spectroscopic measurements help refine our understanding of QED bound state theory, while studying rare decays and the effect of gravitation on positronium aids in the search for new physics phenomena. In biological materials, positronium decays provide information about intermolecular and intramolecular structure, as well as the metabolism of living organisms. This opens up possibilities for positronium imaging in medicine, particularly in positron emission tomography (PET), where positronium atoms are produced during annihilation inside the patient's body. Advanced PET systems offer potential clinical applications of positronium as a biomarker for tissue pathology and oxidation level.
REVIEWS OF MODERN PHYSICS
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Pawel Moskal, Ewelina Kubicz, Grzegorz Grudzien, Eryk Czerwinski, Kamil Dulski, Bartosz Leszczynski, Szymon Niedzwiecki, Ewa L. Stepien
Summary: A new imaging method called ortho-Positronium (o-Ps) imaging was proposed to determine cardiac myxoma by detecting the o-Ps atom generated in the patient's body. The study found that the o-Ps lifetime in myxoma tissue was significantly shorter compared to normal tissue. This method provides a new quantitative approach for in vivo assessment of intracardiac masses on a molecular (nanoscale) level in cardiovascular imaging.
Article
Radiology, Nuclear Medicine & Medical Imaging
S. Sharma, J. Baran, N. Chug, C. Curceanu, E. Czerwinski, M. Dadgar, K. Dulski, K. Eliyan, A. Gajos, N. Gupta-Sharma, B. C. Hiesmayr, K. Kacprzak, L. Kaplon, K. Klimaszewski, P. Konieczka, G. Korcyl, T. Kozik, W. Krzemien, D. Kumar, Sz. Niedzwiecki, D. Panek, S. Parzych, E. Perez del Rio, L. Raczynski, Shivani Choudhary, R. Y. Shopa, M. Skurzok, E. L. Stepien, F. Tayefi, K. Tayefi, W. Wislicki, P. Moskal
Summary: This study aims to determine the registration efficiency of the J-PET tomograph as a function of energy deposition by incident photons and the intrinsic efficiency of the J-PET scanner in detecting photons of different incident energies. The experimental results show that the registration efficiency of the J-PET scanner varies between 20% and 100% in the energy deposition range of 70-270 keV.
Review
Medicine, General & Internal
Ewa L. Stepien, Martyna Durak-Kozica, Pawel Moskal
Summary: Extracellular vesicles (EVs) are released by all cells, both in physiological and pathological conditions. Their molecular charge and composition emerge as possible biomarkers, but EVs may also be considered for other clinical applications. This review discusses the role of other features of EVs, such as their lipid components or composition of glycans that form the EV corona and regulate EV biodistribution and uptake by target cells. The importance of EV electric charge has been discussed as a new insight into EV fate and destination.
POLISH ARCHIVES OF INTERNAL MEDICINE-POLSKIE ARCHIWUM MEDYCYNY WEWNETRZNEJ
(2023)
Review
Mathematical & Computational Biology
Pawel Moskal, Ewa L. Stepien
Summary: This review article discusses the potential use of positronium as a biomarker for hypoxia assessment, highlighting the differences in oxygen partial pressure between healthy and cancer tissues and how it affects the positronium lifetime. It also emphasizes the feasibility of achieving organ-wise positronium imaging with total-body PET systems.
BIO-ALGORITHMS AND MED-SYSTEMS
(2022)
Review
Mathematical & Computational Biology
Ewa L. Stepien, Carina Rzaca, Pawel Moskal
Summary: Extracellular vesicles (EVs) are nano- and micro-sized double-layered membrane entities derived from most cell types and released into biological fluids. Recent advances in the field of EVs and developments of new imaging methods have revealed benefits of radiolabeled EVs in diagnostic and interventional medicine as a potential drug delivery system in theranostics.
BIO-ALGORITHMS AND MED-SYSTEMS
(2022)
Review
Mathematical & Computational Biology
Abass Alavi, Thomas J. Werner, Ewa L. Stepien, Pawel Moskal
Summary: PET imaging is a quantitative modality for assessing disease activity, with revolutionary impact on medical research and practice. Development of novel radiotracers and PET instrumentation has enabled total-body molecular imaging and holds potential for future advancements in understanding molecular and biological bases of disorders.
BIO-ALGORITHMS AND MED-SYSTEMS
(2022)
