Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 182, Issue 3, Pages 990-999Publisher
SPRINGER
DOI: 10.1007/s12010-016-2376-1
Keywords
Poly-gamma-glutamic acid (gamma-PGA); Polypeptides; Bacillus licheniformis; Fermentation; Production kinetics
Funding
- Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University
- Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission
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Bacillus licheniformis TISTR 1010 was used for glutamic acid-independent production of poly-gamma-glutamic acid (gamma-PGA). A fed-batch production strategy was developed involving feedings of glucose, citric acid, and ammonium chloride at specified stages of the fermentation. With the dissolved oxygen concentration controlled at >= 50% of air saturation and the pH controlled at similar to 7.4, the fed-batch operation at 37 degrees C afforded a peak gamma-PGA concentration of 39.9 +/- 0.3 g L-1 with a productivity of 0.926 +/- 0.006 g L-1 h(-1). The observed productivity was nearly threefold greater than previously reported for glutamic acid-independent production using the strain TISTR 1010. The molecular weight of gamma-PGA was in the approximate range of 60 to 135 kDa.
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