Journal
ACS SENSORS
Volume 4, Issue 6, Pages 1691-1700Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b00594
Keywords
point-of-care; paper microfluidics; nucleic acid testing; sensitivity enhancement; dissoluble saline barriers; simulation
Funding
- National Natural Science Foundation of China [51806169]
- National Key R&D Program of China [2018YFC1707700]
- Key Program for Science and Technology Innovative Research Team in Shaanxi Province of China [2017KCT-22]
- Program for Innovative Research Team in Yulin Shaanxi Province of China [2017KJJH-02]
- Shaanxi Young 1000 Talent Program
- Key Program for International S&T Cooperation Projects of Shaanxi [2014KW12-01]
- China Postdoctoral Science Foundation [2017M623167]
- Fundamental Research Funds for the Central Universities [xjj2018256]
- Initiative Postdocs Supporting Program [BX201700186]
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Nucleic acid lateral flow assays (NALFAs) have attracted much attention due to their rapid, robust, simple, and cost-effective features. However, the current NALFAs are still limited by low sensitivity because of the poor understanding and control of the underlying complex flow and reaction processes. Although enormous efforts have been devoted to enhancing detection sensitivity of NALFAs, developing simple NALFAs with high sensitivity remains difficult. Thus, we proposed a novel physical-chemical coupling method using dissoluble saline barriers and developed the corresponding mathematical model to better understand the underlying processes to enhance the NALFA sensitivity. Through optimizing the design parameters (e.g., saline barriers patterns, volume, and concentrations) experimentally and numerically, we achieved the highest 10-fold sensitivity enhancement for detection of nucleic acids (including HBV, Staphylococcus aureus, and salmonella as model targets) using this method. The physical-chemical coupling method offers a facile strategy for developing highly sensitive NALFAs.
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