Article
Nutrition & Dietetics
Ilse H. de Lange, Charlotte van Gorp, Kimberly R. I. Massy, Lilian Kessels, Nico Kloosterboer, Ann Bjornshave, Marie Stampe Ostenfeld, Jan G. M. C. Damoiseaux, Joep P. M. Derikx, Wim G. van Gemert, Tim G. A. M. Wolfs
Summary: Many whey proteins, peptides, and protein-derived amino acids can improve gut health through their anti-oxidant, anti-microbial, barrier-protective, and immune-modulating effects. This study investigated the effects of whey protein fractions with different degrees of enzymatic hydrolysis on the intestinal epithelium, as well as their anti-microbial and immune effects. The results highlight the importance of hydrolysis degree in influencing the biological effects of whey protein fractions, and provide new insights for improving immune tolerance and promoting intestinal health.
Article
Chemistry, Analytical
Miki Matsumoto, Yuya Morimoto, Toshiro Sato, Shoji Takeuchi
Summary: In this study, a microfluidic organoid-trapping device was proposed to immobilize and stimulate human intestinal organoids. The device successfully trapped and cultured human intestinal organoids, and demonstrated the ability to induce organoid fusion and regulate their surface structures using fluidic shear stress.
Review
Gastroenterology & Hepatology
Xiao Han, Matthias A. Mslati, Emily Davies, Yan Chen, Joannie M. Allaire, Bruce A. Vallance
Summary: Intestinal organoids play a crucial role in gastrointestinal research, particularly in studying nontransformed intestinal epithelial cells and their involvement in diseases like inflammatory bowel disease and colon cancer. Research on the advantages of using organoids derived from adult stem cells, as well as the impact of biopsy location and patient age on organoids and their interactions with microbes, has made significant progress. Various methods of introducing bacteria in a relevant manner and assessing key outputs using these models have also been addressed.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Review
Immunology
Lila S. Nolan, Megan T. Baldridge
Summary: This article describes the use of human intestinal enteroids (HIEs) to study the mechanisms of human enteric viral infections in vitro, as well as the role of interferons in antiviral immune responses. These research findings help to reveal important aspects of enteric viral infections and facilitate the development of treatments and vaccines.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Veterinary Sciences
Amy Stieler Stewart, Cecilia R. Schaaf, Brittany Veerasammy, John M. Freund, Liara M. Gonzalez
Summary: This study describes the isolation and culture of equine intestinal epithelial stem cells (ISCs) after delayed tissue storage. The resulting enteroids showed minimal differences even after 24-48 hours of storage, suggesting that ISCs could be isolated and stored for several days for future experimental and therapeutic use. Cryopreserved enteroid fragments showed improved growth efficiency compared to cryopreserved crypts, indicating potential storage techniques for ISCs.
BMC VETERINARY RESEARCH
(2022)
Article
Pediatrics
Shan He, Gang Liu, Xueping Zhu
Summary: This study demonstrates the beneficial effects of human breast milk-derived exosomes in preventing necrotizing enterocolitis by reducing inflammation and injury, and restoring intestinal tight-junction proteins.
PEDIATRIC RESEARCH
(2021)
Article
Veterinary Sciences
Brigid Orr, Kate Sutton, Sonja Christian, Tessa Nash, Helle Niemann, Lone Lind Hansen, Mike J. McGrew, Stina Rikke Jensen, Lonneke Vervelde
Summary: The 2D enteroids model developed in chicken recapitulates major differentiated cell lineages and shows tight cell layer formation with minimal paracellular flux, robust epithelial integrity, and appropriate innate immune responses. This model serves as a valuable tool for studying intestinal cell biology and innate immune function, with potential applications in screening of various compounds.
VETERINARY RESEARCH
(2021)
Review
Veterinary Sciences
Tessa Nash, Lonneke Vervelde
Summary: There is a growing interest in studying the avian intestine and its role in dietary components, probiotic microorganisms, and infectious diseases. However, the lack of physiologically relevant models has hindered research in this field. This review discusses various in vitro tools that aim to mimic the intestinal environment, from simple cell lines to complex 3D enteroids, highlighting their benefits, limitations, and potential applications in intestinal biology and pathology research. The review also emphasizes the need for improved models that incorporate microbiota and a dynamic mechanical environment.
Article
Agriculture, Dairy & Animal Science
Dan Zhao, Morgan B. Farnell, Michael H. Kogut, Kenneth J. Genovese, Robert S. Chapkin, Laurie A. Davidson, Luc R. Berghman, Yuhua Z. Farnell
Summary: This study successfully generated crypt-derived organoids from chicken intestines and optimized conditions for their growth and cryopreservation. The developed model will contribute to understanding host-pathogen interactions and discovering pathogen intervention strategies in poultry.
Article
Nutrition & Dietetics
Samuel Blake Mitchell, Yu-Han Hung, Trista Lee Thorn, Jiaqi Zou, Filiz Baser, Sukru Gulec, Celeste Cheung, Tolunay Beker Aydemir
Summary: This study aims to investigate the impact of high sucrose consumption on zinc homeostasis and intestinal permeability, and its correlation with the development of chronic diseases.
FRONTIERS IN NUTRITION
(2023)
Article
Immunology
Xiao-Lin Yan, Xiao-Chen Liu, Yu-Ni Zhang, Ting-Ting Du, Qing Ai, Xiong Gao, Jing-Li Yang, Lei Bao, Lu-Quan Li
Summary: This study found that succinate plays an important role in the pathogenesis and severity of necrotizing enterocolitis, and the activation of the HIF-1α signaling pathway may lead to disease progression.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Jorik M. van Rijn, Jens Eriksson, Jana Gruttner, Magnus Sundbom, Dominic-Luc Webb, Per M. Hellstrom, Staffan G. Svard, Mikael E. Sellin
Summary: The study focuses on dynamic interactions between pathogenic microbes and host tissues, utilizing live-cell microscopy to visualize microbe behavior on intestinal epithelial cell (IEC) layers. By imaging without fluorescent labels, the method provides high-resolution observation of Salmonella and Giardia interactions on the epithelial surface, revealing pathogen-specific swimming patterns and stages of infection cycles previously unrecognized. This relatively inexpensive approach bridges the divide between technical requirements for live-cell imaging and physiological infection niche parameters.
Article
Microbiology
Jorik M. van Rijn, Jens Eriksson, Jana Gruttner, Magnus Sundbom, Dominic-Luc Webb, Per M. Hellstrom, Staffan G. Svard, Mikael E. Sellin
Summary: Understanding the dynamic interactions between individual microbes and host cells is crucial for infectious disease research. A new method for live-cell imaging of microbial infection dynamics on human intestinal epithelial cell layers has been developed, providing insights into the behavior of Salmonella and Giardia pathogens.
Article
Virology
Ruchika Shakya, Alejandro Jimenez-Melendez, Lucy J. Robertson, Mette Myrmel
Summary: This study established bovine enteroids as an in vitro replication system for BCoV and found that the expression of pro-inflammatory genes remained unchanged, while the expression of other immune genes was significantly downregulated in response to BCoV infection.
Article
Infectious Diseases
K. Brooke Snyder, Alena Golubkova, Tyler Leiva, Chase Calkins, Heather Liebe, Camille Schlegel, Catherine J. Hunter
Summary: Necrotizing enterocolitis (NEC) is a devastating disease of premature neonates with substantial morbidity and mortality. This study found that patients with NEC have an increased hyperinflammatory intestinal response compared with those without NEC.
SURGICAL INFECTIONS
(2023)
Article
Surgery
Christie L. Buonpane, Guillermo J. Ares, Ethan G. Englert, Irene Helenowski, Valeria C. Cohran, Catherine J. Hunter
AMERICAN JOURNAL OF SURGERY
(2018)
Article
Pediatrics
Christie Buonpane, Timothy B. Lautz, Yue-Yung Hu
JOURNAL OF PEDIATRIC SURGERY
(2019)
Article
Pediatrics
Guillermo J. Ares, Christie Buonpane, Irene Helenowski, Marleta Reynolds, Catherine J. Hunter
PEDIATRIC SURGERY INTERNATIONAL
(2019)
Article
Surgery
Steven T. Papastefan, Christie Buonpane, Guillermo Ares, Beshoy Benyamen, Irene Helenowski, Catherine J. Hunter
JOURNAL OF SURGICAL RESEARCH
(2019)
Article
Multidisciplinary Sciences
Guillermo Ares, Christie Buonpane, John Sincavage, Carrie Yuan, Douglas R. Wood, Catherine J. Hunter
SCIENTIFIC REPORTS
(2019)
Article
Surgery
Christie Buonpane, Enobong Efiong, Marie Hunsinger, Marcus Fluck, Mohsen Shabahang, Jeffrey Wild, Kristen Halm, Kevin Long, Christopher Buzas, Joseph Blansfield