Article
Cell Biology
Noreen Mumtaz, Amel Dudakovic, Asha Nair, Marijke Koedam, Johannes P. T. M. van Leeuwen, Marion P. G. Koopmans, Barry Rockx, Andre J. J. van Wijnen, Bram C. J. van der Eerden
Summary: Arboviruses like Zika virus affect bone development by targeting osteoblasts and altering bone remodeling. In this study, the researchers investigated the effects of Zika virus infection on mesenchymal stromal cells (MSCs) at different stages of osteogenesis. They found that late-stage infected MSCs showed elevated osteogenic markers, calcium content, and altered gene expression compared to early-stage infected MSCs.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yoon-Young Go, Chan-Mi Lee, Sung-Won Chae, Jae-Jun Song
Summary: The study demonstrated the potential of trophoblast-derived conditioned medium (TB-CM) in promoting osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) and inhibiting adipogenesis. By activating biological processes such as signaling pathways, the trophoblast-derived secretomes show promise for clinical interventions in bone regeneration and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Antonia Ressler, Maja Antunovic, Laura Teruel-Biosca, Gloria Gallego Ferrer, Slaven Babic, Inga Urlic, Marica Ivankovic, Hrvoje Ivankovic
Summary: Ionic substitutions in composite materials have shown promising potential in enhancing the biological performance for bone tissue engineering applications. This study focused on multi-substituted organic/inorganic scaffolds and demonstrated that these scaffolds have high stability and can promote osteogenic potential of human mesenchymal stem cells. Results highlighted the beneficial effect of ionic substitutions on cells and tissues.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Vitale Miceli, Alessandro Bertani, Cinzia Maria Chinnici, Matteo Bulati, Mariangela Pampalone, Giandomenico Amico, Claudia Carcione, Eva Schmelzer, Jorg C. Gerlach, Pier Giulio Conaldi
Summary: The study showed that the conditioned medium of human amnion-derived MSCs can alleviate the effects of lung IRI by improving the efficacy of EVLP, preserving the viability of alveolar epithelial cells and delaying apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Ying Zhang, Ling Ling, Arya Ajay D. O. Ajayakumar, Yating Michelle Eio, Andre J. van Wijnen, Victor Nurcombe, Simon M. Cool
Summary: This study reveals a positive correlation between FGFR2 expression and osteogenic potential in human adult bone marrow-derived MSCs. Knockdown of FGFR2 leads to downregulation of pro-osteogenic genes and upregulation of pro-adipogenic genes, suppressing osteogenesis. Furthermore, FGFR2 knockdown results in upregulation of EZH2, an enzyme that regulates MSC lineage commitment and suppresses osteogenesis under osteogenic induction.
Article
Biochemistry & Molecular Biology
Shuang Zhang, Jeroen van de Peppel, Marijke Koedam, Johannes P. T. M. van Leeuwen, Bram C. J. van der Eerden
Summary: In this study, TNS3 was found to be involved in the fate decision of human bone marrow-derived stromal cells (BMSCs), regulating osteogenesis and adipogenesis through the control of RhoA activity and cytoskeletal reorganization. The findings highlight the crucial role of TNS3 in bone metabolism.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Oliwia Milek, Dino Tur, Lucia Ahcin, Olha Voitseshyna, Christian Behm, Oleh Andrukhov
Summary: This study investigated the impact of osteogenic differentiation on the immunomodulatory properties of hPDL-MSCs, and found that osteogenic differentiation reduced the ability of hPDL-MSCs to suppress CD4(+) T lymphocyte proliferation and enhanced this ability in the presence of IL-1 beta. Additionally, the expression and activity of indoleamine-2,3-dioxygenase and interleukin-8 were also affected by the differentiation process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Zhenghai Zhang, Lulu Gong, Min Li, Guoshuai Wei, Yan Liu
Summary: This study investigated the effects of NF and FFF scaffolds on the behavior of hBMSCs using MFCS, IFS, and SVM, revealing that NF scaffolds induce the expression of more osteogenic-related genes in hBMSCs. Enrichment analysis of common genes suggests that NF scaffolds may enhance the osteogenic differentiation ability of hBMSCs.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Materials Science, Multidisciplinary
Jong Bo Park, Ji Yeon Ahn, Woo Sub Yang, Seong Chae Park, Young Jin Jung, Myungook Lee, Byung Hee Hong, Jeong Mook Lim
Summary: This study investigated the effect of nanoscale spacing by stacked graphene film on osteogenic differentiation of human adipose-derived mesenchymal stromal cells. The results showed that osteogenic differentiation was promoted as the stacking layer increased, with the most significant effects observed in the three layers. The study demonstrated that multilayer-stacked graphene created nanoscale spaces promote calcium deposit and cytoskeletal integrity in hADSC-related, in vitro-osteogenesis.
Article
Biochemistry & Molecular Biology
Giulia Montagna, Giuseppe Pani, Dani Flinkman, Francesco Cristofaro, Barbara Pascucci, Luca Massimino, Luigi Antonio Lamparelli, Lorenzo Fassina, Peter James, Eleanor Coffey, Giuseppina Rea, Livia Visai, Angela Maria Rizzo
Summary: For the first time, this study characterizes the morphological, biochemical, and molecular changes in human bone marrow stromal cells during long-term simulated microgravity exposure. It reveals that simulated microgravity affects osteogenic differentiation and energy metabolism, and identifies dysregulated proteins that may be potential targets for future translational projects. The findings provide insights into the cell response to simulated microgravity and suggest the application of nanomaterials for mitigating the effects of microgravity.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jing Wang, Qingchen Qiao, Yaxi Sun, Wenting Yu, Jiran Wang, Minjia Zhu, Kai Yang, Xiaofeng Huang, Yuxing Bai
Summary: This study investigated the impact of cell culture density on stem cell proliferation, osteoblastogenesis, and regulation. The results showed that the proliferation rate of human periodontal ligament stem cells (hPDLSCs) decreased with an increase in initial plating density. After osteogenic differentiation, hPDLSCs initially plated at a density of 2 x 10(4) cells/cm(2) showed the highest expression of OPG and RUNX2, as well as the highest OPG/RANKL ratio and cell calcium concentration. Furthermore, exosomes derived from hPDLSCs cultured at different initial cell densities were found to regulate osteoblastic differentiation of human bone marrow stromal cells (hBMSC), with the 2 x 10(4) cells/cm(2) initial cell density group showing the highest expression of OPG, OCN, RUNX2, and osterix, as well as the highest average calcium concentration. This provides a new perspective for the clinical application of stem cell osteogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laura Alves, Vanessa Machado, Joao Botelho, Jose Joao Mendes, Joaquim M. S. Cabral, Claudia L. da Silva, Marta S. Carvalho
Summary: Cell-based therapies using PDLSC may be a promising alternative to MSC(M) and MSC(AT) for periodontal regeneration, as PDLSC showed enhanced proliferative and osteogenic potential. PDLSC expressed osteogenic markers and had higher calcium content and upregulated osteogenic/periodontal genes compared to MSC(M) and MSC(AT). CD146 was specifically expressed by PDLSC, which also had a higher proliferative capacity. However, alkaline phosphatase activity did not increase in PDLSC.
Article
Engineering, Industrial
Marco Sorgato, Enrica Guidi, Maria Teresa Conconi, Giovanni Lucchetta
Summary: Surface nanostructuring of bioresorbable polymers shows promise as a solution for tissue regeneration therapies, with optimized nanopillar design parameters inducing osteogenic differentiation of human stem cells. Both in vitro and in vivo cell experiments confirm the potential application in tissue regeneration, highlighting the need for independent optimization of nanopillar diameter, height, and spacing.
CIRP ANNALS-MANUFACTURING TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kenny Man, Liam Lawlor, Lin-Hua Jiang, Xuebin B. Yang
Summary: The study showed that the selective inhibitor MI192 for HDAC2 and 3 can enhance the osteogenic capacity of hDPSCs and promote bone regeneration. Pre-treatment with MI192 enhances epigenetic functionality, induces cell cycle arrest, and upregulates expression of osteoblast-related genes in hDPSCs during osteogenic differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Yu-Jin Kim, Sang Ho Lee, Jisoo Im, Jihun Song, Han Young Kim, Suk Ho Bhang
Summary: This study demonstrates that light irradiation can enhance the expression of angiogenic paracrine factors in spheroids of human adipose-derived stem cells (hADSCs). Conditioned medium derived from light-irradiated hADSCs shows improved viability, migration, and angiogenesis in cells. In vivo experiments using a mouse hindlimb ischemia model also confirm the increased angiogenesis induced by conditioned medium derived from light-irradiated hADSCs.
COMMUNICATIONS BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Marco Govoni, Claudio Muscari, Francesca Bonafe, Paolo Giovanni Morselli, Marilisa Cortesi, Dante Dallari, Emanuele Giordano
Summary: This study demonstrated that under very low oxygen tension, human adipose-derived mesenchymal stem cells can commit to chondrogenic differentiation in just 2 days, with p38 MAPK signaling pathway playing a crucial role in the transcriptional regulation of chondrogenesis.
ADVANCES IN MEDICAL SCIENCES
(2021)
Article
Engineering, Biomedical
Alice Pasini, Joseph Lovecchio, Marilisa Cortesi, Chiara Liverani, Chiara Spadazzi, Laura Mercatali, Toni Ibrahim, Emanuele Giordano
Summary: This study suggests a breast cancer cell culture model that combines a 3D environment and fluid perfusion, improving cell distribution viability and uniformity while inducing morphological, functional, and molecular remodeling of cancer cells.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Biophysics
Marilisa Cortesi, Andrea Samore, Joseph Lovecchio, Roberta Ramilli, Marco Tartagni, Emanuele Giordano, Marco Crescentini
Summary: This study focuses on mineralization in 3D cell cultures and proposes electrical impedance tomography (EIT) as a non-destructive approach. By developing an integrated hardware/software system for acquiring experimental data and reconstructing conductivity maps, the functionality of the system is validated and biological variability is limited.
PHYSIOLOGICAL MEASUREMENT
(2021)
Article
Pharmacology & Pharmacy
Erwin Pavel Lamparelli, Joseph Lovecchio, Maria Camilla Ciardulli, Valentina Giudice, Tina P. Dale, Carmine Selleri, Nicholas Forsyth, Emanuele Giordano, Nicola Maffulli, Giovanna Della Porta
Summary: This study demonstrated the successful chondrogenic differentiation of human Bone Marrow Mesenchymal Stem Cells within a collagen matrix functionalized with PLGA-MCs under dynamic perfusion conditions. The findings highlight the importance of growth factor controlled delivery in 3D hydrogel scaffolds for tissue engineering applications.
Article
Pharmacology & Pharmacy
Maria Camilla Ciardulli, Joseph Lovecchio, Pasqualina Scala, Erwin Pavel Lamparelli, Tina Patricia Dale, Valentina Giudice, Emanuele Giordano, Carmine Selleri, Nicholas Robert Forsyth, Nicola Maffulli, Giovanna Della Porta
Summary: This study described a novel bio-functionalized 3D fibrous construct for studying the tenogenic potential events of mesenchymal stem cells. The structure showed promising results in promoting teno-regenerative events and significant gene upregulation effects were observed.
Article
Biotechnology & Applied Microbiology
Pasqualina Scala, J. Lovecchio, E. P. Lamparelli, R. Vitolo, V Giudice, E. Giordano, C. Selleri, L. Rehak, N. Maffulli, G. Della Porta
Summary: An in-vitro model of human bone marrow mesenchymal stem cells (hBM-MSCs) myogenic commitment was developed using static co-seeding and perfused culture systems. Static co-seeding showed a significant increase in gene expression, while the myogenic induction ability of hSkMs on hBM-MSCs under perfusion conditions was mainly exerted through paracrine effect. The dynamic culture also resulted in a significant increase in anti-inflammatory cytokine gene expression, confirming the immunomodulatory activity of hBM-MSCs along with commitment events.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
J. Lovecchio, V Betti, M. Cortesi, E. Ravagli, S. Severi, E. Giordano
Summary: Monitoring the maturation of bone tissue engineered constructs is crucial for ensuring their quality. Traditional destructive methods require sacrificing samples, while light spectroscopy-based methods provide non-destructive monitoring. In this study, a miniaturized and inexpensive custom-made spectrometer device is proposed for real-time/in-line analysis and integration with bioreactor systems.
ROYAL SOCIETY OPEN SCIENCE
(2022)
Article
Chemistry, Physical
Joseph Lovecchio, Marilisa Cortesi, Marco Zani, Marco Govoni, Dante Dallari, Emanuele Giordano
Summary: 3D printing has provided new opportunities for the fabrication of biocompatible hybrid pseudo-tissues. This study focuses on modifying a commercial 3D printer into a 3D bioplotter to control scaffold fiber thickness and porosity. The results demonstrate the feasibility of printing hydrogels commonly used for cell culture and tissue engineering applications.
Article
Biotechnology & Applied Microbiology
Pasqualina Scala, Paola Manzo, Erwin Pavel Lamparelli, Joseph Lovecchio, Maria Camilla Ciardulli, Valentina Giudice, Carmine Selleri, Emanuele Giordano, Laura Rehak, Nicola Maffulli, Giovanna Della Porta
Summary: In this study, a 3D environment was created using fibrin scaffold and two cell populations. The addition of peripheral blood mononuclear cells (PBMCs) to the culture system significantly influenced myogenesis. The best cell ratio for the 3D culture was found to be 1:1 (BM-MSCs plus SkMs:PBMCs), resulting in enhanced viability and myogenic gene expression.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Physical
Gabriele Boretti, Emanuele Giordano, Mariana Ionita, George Mihail Vlasceanu, olafur Eysteinn Sigurjonsson, Paolo Gargiulo, Joseph Lovecchio
Summary: A study was conducted to investigate if the osteogenic potential of CGG scaffolds without adding GO could be improved by enhancing medium diffusion in a 3D culture. The study used human bone-marrow-derived stem cells (hBMSC) seeded in 3D CGG scaffolds and found that perfusion flow in dynamic culture promoted cell differentiation and enhanced extracellular matrix (ECM) mineralization compared to static culture. This research reveals a new perspective on the clinical application of CGG scaffolds without GO.
Meeting Abstract
Cell & Tissue Engineering
Erwin Pavel Lamparelli, Joseph Lovecchio, Rossella Vitolo, Emanuele Giordano, Carmine Selleri, Nicola Maffulli, Giovanna Della Porta
TISSUE ENGINEERING PART A
(2022)