Journal
FRONTIERS IN MICROBIOLOGY
Volume 10, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2019.00788
Keywords
bla(NDM-1); ST405; Escherichia coli; IncN2; China; carbapenem-resistant Enterobacteriaceae
Categories
Funding
- Focus on Research and Development Plan in Shandong Province [2016GSF201078]
- Jinan Science and Technology Development Plan [201805021]
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We report a bla(NDM)-carrying ST405 Escherichia coli recovered from the abdominal fluid of a patient in Shandong, China. This strain belonged to the high-risk phylogenetic group D and carried the virulence genes, papG II, papG III, papC, and iroN. In addition to bla(NDM-1), this isolate carried the quinolone resistance gene acc(6')-Ib and extended-spectrum beta-lactamase (ESBL) genes bla(CTX-M-)(15) and bla(CTX-M-)(14). bla(NDM-1) was located on a 41 Kb IncN2 self-transmissible plasmid. The IncN2 plasmid named as pJN24NDM1 was fully sequenced and analyzed. Genome comparative analysis showed that IncN2 plasmids harboring carbapenem-resistance genes possessed conserved backbones and variable accessory regions. Phylogenetic analysis of 87 IncN plasmids based on orthologous genes indicated that 9 IncN2 plasmids fell into one phylogenetic Glade, while 4 IncN3 plasmids were in two phylogenetic clades of the 74 IncN1 plasmids. The presence of IncN2 plasmids harboring bla(NDM) in the high-risk clone ST405 E. coli raises serious concerns for its potential of dissemination.
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