Article
Multidisciplinary Sciences
Shaobo Wei, Xia Li, Zefu Lu, Hui Zhang, Xiangyuan Ye, Yujie Zhou, Jing Li, Yanyan Yan, Hongcui Pei, Fengying Duan, Danying Wang, Song Chen, Peng Wang, Chao Zhang, Lianguang Shang, Yue Zhou, Peng Yan, Ming Zhao, Jirong Huang, Ralph Bock, Qian Qian, Wenbin Zhou
Summary: In this study, a photosynthesis-related transcription factor, OsDREB1C, was identified and found to regulate various functions such as photosynthetic capacity, nitrogen utilization, and flowering time in rice. Field trials with OsDREB1C-overexpressing rice showed increased yield, shortened growth duration, and improved nitrogen use efficiency.
Article
Multidisciplinary Sciences
Aaron N. Brooks, Amanda L. Hughes, Sandra Clauder-Muenster, Leslie A. Mitchell, Jef D. Boeke, Lars M. Steinmetz
Summary: This study investigates the effects of sequence features and transcriptional context on RNA transcript isoform expression. Using synthetic yeast strains with rearranged genes, the researchers discovered that neighboring transcription also influences gene expression levels and transcript isoform boundaries. They identified features of transcriptional context that can predict these alterations and successfully engineered a synthetic circuit where transcript length is controlled by neighboring transcription.
Article
Genetics & Heredity
Shen Tang, Ping Men, Wei Zhang, Hui Li, Zhimin Li, Xuenian Huang, Xuefeng Lu
Summary: The secondary metabolism in filamentous fungi is highly sensitive to the environment, with biosynthetic gene clusters of secondary metabolites often being silent under laboratory conditions. This study successfully identified a highly conserved naphthopyrone PKS gene and determined its product to be naphthoquinones through activation of the silent biosynthetic pathway. These naphthoquinones are soluble pigments that can be secreted into the agar medium and culture broth, resulting in darker coloration of the activated mutant compared to the parental strain.
FUNGAL GENETICS AND BIOLOGY
(2022)
Article
Immunology
Noam Kadouri, Tal Givony, Shir Nevo, Joschka Hey, Shifra Ben Dor, Golda Damari, Bareket Dassa, Jan Dobes, Dieter Weichenhan, Marion Bahr, Michelle Paulsen, Rebecca Haffner-Krausz, Marcus A. Mall, Christoph Plass, Yael Goldfarb, Jakub Abramson
Summary: This study reveals the importance of FOXN1 in the development of thymic epithelial cells and hair follicle cells, and identifies key regulatory regions and transcription factors involved in its expression.
SCIENCE IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yi-Hua Huang, Jia-Qi Han, Biao Ma, Wu-Qiang Cao, Xin-Kai Li, Qing Xiong, He Zhao, Rui Zhao, Xun Zhang, Yang Zhou, Wei Wei, Jian-Jun Tao, Wan-Ke Zhang, Wenfeng Qian, Shou-Yi Chen, Chao Yang, Cui-Cui Yin, Jin-Song Zhang
Summary: Through the analysis of an ethylene-response mutant mhz9, we identified a glycine-tyrosine-phenylalanine (GYF) domain protein MHZ9, which positively regulates ethylene signaling at the translational level in rice. MHZ9 is localized in RNA processing bodies. Our study reveals that MHZ9 interacts with OsEIN2 and directly binds to OsEBF1/2 mRNAs to inhibit translation, leading to the accumulation of OsEIL1 transcription factor and activation of downstream signaling. Ribo-seq analysis indicates that MHZ9 is required for the regulation of approximately 90% of genes affected by ethylene at the translational level.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Bin Zhang, Zhi-yi Liu, Rui Wu, Cheng-ming Zhang, Kuan Cao, Wen-gang Shan, Zhen Liu, Ming Ji, Zi-lu Tian, Gautam Sethi, Heng-liang Shi, Ren-hao Wang
Summary: CTR9, a scaffold protein of the PAF1 complex, plays essential roles in hepatocellular carcinoma by promoting cell proliferation, invasion, and migration. CTR9 also promotes HCC development by enhancing the transcription of the oncogene PEG10. Targeting CTR9 may provide a novel therapeutic strategy for HCC.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Plant Sciences
Yunan Lu, Jianbo Qiu, Shufang Wang, Jianhong Xu, Guizhen Ma, Jianrong Shi, Zenghai Bao
Summary: In this study, phylogenetic analyses were conducted on 186 Fusarium isolates from rice and soybean, revealing 12 different species with the majority belonging to the Incarnatum clade of the FIESC species. Host plants influenced the population composition, with specific species dominating in rice and soybean. Analysis of Tri gene sequences showed consistent results with phylogenetic markers, and the main mycotoxins produced were Type-A and -B trichothecenes, with Diacetoxyscirpenol being detected in all strains and other toxins in specific FIESC strains. These findings provide valuable information for the risk assessment of mycotoxins in agricultural products.
Article
Biochemical Research Methods
Nolan J. O'Connor, Antoni E. Bordoy, Anushree Chatterjee
Summary: Antisense transcription plays a widespread role in influencing gene expression through transcriptional interference (TI), and the processivity control of RNA polymerase (RNAP) can be used to tune TI and enhance gene repression, suggesting potential applications in synthetic biology. In this study, three distinct antitermination strategies were applied to control TI and design minimal genetic NAND and NOR logic gates, demonstrating the importance of processivity control for strong TI and highlighting TI's potential in synthetic biology.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Plant Sciences
Yunfei Wu, Leilei Wang, Ebenezer Ottopah Ansah, Wangmenghan Peng, Weiyang Zhang, Peng Li, Gynheung An, Fei Xiong
Summary: Photosynthetic tissues are dynamic structures that rely on the balance of self-maintenance and supporting sink tissues. This study reveals the key role of OsDOF11 in mediating sucrose transport and cytokinin signaling in rice. OsDOF11 regulates sucrose transport by inducing the expression of sucrose transporter genes, and directly binds to the promoter regions of OsCKX4 to control cytokinin metabolism. These findings uncover a feedback loop for maintaining tissue homeostasis.
Article
Plant Sciences
Gouranga Upadhyaya, Arup Das, Sudipta Ray
Summary: The R2R3 type MYB transcription factors play a crucial role in controlling flavonoid production in plants, such as anthocyanin and proanthocyanin. By studying the relationship between OsC1 and anthocyanin biosynthesis as well as oxidative stress tolerance in rice, it was found that OsC1 may act as a transcriptional regulator of ABP genes, enhancing plant tolerance to oxidative stresses.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Kompal Gaur, Nirmaljit Kaur, Anju Bala Sharma, Anuj Choudhary
Summary: In this study, the potential of seed priming with plant growth substances on bakanae infected seedlings of aromatic rice varieties Pusa Basmati 1121 and Pusa Basmati 1509 was evaluated. Seed priming treatments resulted in improved germination, weight, vigour, chlorophyll and carotenoid content. The treatments also reduced disease incidence and visible symptoms while enhancing field emergence and biochemical attributes. This study can contribute to the understanding of the biochemical and molecular mechanisms involved in plant growth substance interactions during bakanae infestation in rice.
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Karolina Kotecka, Adam Kawalek, Magdalena Modrzejewska-Balcerek, Jan Gawor, Karolina Zuchniewicz, Robert Gromadka, Aneta Agnieszka Bartosik
Summary: Pseudomonas aeruginosa PA3973 is an uncharacterized transcriptional regulator that influences the survival ability and stress response of the pathogen by controlling the expression of other genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Arezoo Rahimi, Omid Karami, Angga Dwituti Lestari, Tobias de Werk, Petra Amakorova, Dongbo Shi, Ondrej Novak, Thomas Greb, Remko Offringa
Summary: AHL15 transcriptional regulator plays an important role in controlling vascular cambium activity and promoting secondary xylem formation. Overexpression of AHL15 can induce woody inflorescence stems in herbaceous plants. AHL15 induces vascular cambium activity by repressing SOC1 and FUL transcription factors.
Article
Biochemistry & Molecular Biology
Nayeon Ki, Jinshil Kim, Inseong Jo, Yongseong Hyun, Sangryeol Ryu, Nam-Chul Ha
Summary: This study determined the crystal structure of the regulatory domain of RipR from the intracellular pathogen Salmonella, revealing the typical dimeric arrangement. Isothermal titration calorimetry experiments identified isocitrate as the physiological ligand of RipR, which increases intracellular level in response to itaconic acid stress. Additionally, 3-phenylpropionic acid was found to significantly reduce bacterial resistance to an itaconic acid challenge.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Kenichiro Abe, Tamas Schauer, Maria-Elena Torres-Padilla
Summary: This study investigates the regulation of transcription during early mammalian development by examining the distribution of Serine 5 and Serine 2-phosphorylated Pol II on the genome in mouse embryos. The study reveals that Serine 2-phosphorylated Pol II appears before gene activation, potentially priming gene expression. CDK9 and SPT5 are identified as major regulators of zygotic genome activation.