Journal
STEM CELLS TRANSLATIONAL MEDICINE
Volume 8, Issue 8, Pages 844-857Publisher
OXFORD UNIV PRESS
DOI: 10.1002/sctm.18-0182
Keywords
Bone engineering; Mesenchymal stem cells; Hypoxia; Intramembranous ossification; Calvaria
Categories
Funding
- University Paris Descartes
- Fondation des Gueules Cassees
- National French Agency for research (ANR PulpCell 2014-2018)
- Fondation de la Recherche Medicale [FRM DGE20111123012]
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The craniofacial area is prone to trauma or pathologies often resulting in large bone damages. One potential treatment option is the grafting of a tissue-engineered construct seeded with adult mesenchymal stem cells (MSCs). The dental pulp appears as a relevant source of MSCs, as dental pulp stem cells display strong osteogenic properties and are efficient at bone formation and repair. Fibroblast growth factor-2 (FGF-2) and/or hypoxia primings were shown to boost the angiogenesis potential of dental pulp stem cells from human exfoliated deciduous teeth (SHED). Based on these findings, we hypothesized here that these primings would also improve bone formation in the context of craniofacial bone repair. We found that both hypoxic and FGF-2 primings enhanced SHED proliferation and osteogenic differentiation into plastically compressed collagen hydrogels, with a much stronger effect observed with the FGF-2 priming. After implantation in immunodeficient mice, the tissue-engineered constructs seeded with FGF-2 primed SHED mediated faster intramembranous bone formation into critical size calvarial defects than the other groups (no priming and hypoxia priming). The results of this study highlight the interest of FGF-2 priming in tissue engineering for craniofacial bone repair.
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