Article
Environmental Sciences
Anup Kumar Singh, Ajay Kumar Pandey, Mohit Kumar, Tanushree Paul, Naseem A. Gaur
Summary: Due to the availability of abundant feedstocks and the environmentally friendly nature of microbial production processes, the production of potential value-added products from different lignocellulosic biomass is becoming more common. In this study, a potential yeast isolate called Candida tropicalis K2 was identified for xylitol production. The isolate showed the highest potential for xylitol production and tolerance to inhibitors compared to other tested isolates. Under specific conditions, C. tropicalis K2 produced high yields of xylitol in batch fermentation, and even higher yields when glycerol was used as a co-substrate or non-detoxified hydrolysates were used. This finding suggests that C. tropicalis K2 is a promising candidate for xylitol production from various lignocellulosic biomass sources.
ENVIRONMENTAL TECHNOLOGY
(2022)
Review
Energy & Fuels
Vasundhara Jain, Sanjoy Ghosh
Summary: Lignocellulosic biomass is a significant renewable energy source, consisting mainly of cellulose, hemicellulose, and lignin, which can be transformed into valuable chemical products. Xylitol, a high-value product derived from lignocellulosic biomass, currently produced chemically, has the potential for biotechnological production to reduce costs and utilize waste. Advances in yeast-based xylitol production are focused on in this review, highlighting pretreatment methods, fermentation strategies, and product recovery.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Biotechnology & Applied Microbiology
Juliana P. Galhardo, Andre P. Piffer, Mateus B. Fiamenghi, Guilherme Borelli, Duguay R. M. da Silva, Adrielle A. Vasconcelos, Marcelo F. Carazzolle, Goncalo A. G. Pereira, Juliana Jose
Summary: A previously ignored yeast enzyme, XylDH, is found to be an important factor in yeast metabolism and crucial for improving second-generation ethanol production. The utilization of D-xylose by yeasts is crucial for enhancing second-generation ethanol production, but industrial yeast strains are unable to consume D-xylose. Through analysis of the XylDH gene family, researchers have provided evidence of the significance of XylDH in D-xylose-related phenotypes in yeasts.
FEMS YEAST RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
N. Kusumawati, S. H. Sumarlan, E. Zubaidah, A. K. Wardani
Summary: This study aims to isolate xylose-utilizing yeasts and analyze their fermentation activity to produce xylitol and ethanol, as well as their ability to grow in liquid hydrolysate produced from pretreated lignocellulosic biomass. Several yeast isolates were found to be able to grow on xylose as a carbon source and produce xylitol and ethanol. These isolated yeast strains have the potential to be used in the bioconversion process of lignocellulosic biomass waste.
BIORESOURCES AND BIOPROCESSING
(2023)
Article
Biochemistry & Molecular Biology
Alejandra Karina Estrada-Avila, Juan Carlos Gonzalez-Hernandez, Martha Calahorra, Norma Silvia Sanchez, Antonio Pena
Summary: Six different yeasts were studied for their metabolism of glucose and xylose. Only K. marxianus and M. guilliermondii were able to produce ethanol from xylose. All strains could produce xylitol from xylose, with K. marxianus, M. guilliermondii, C. lusitaniae, and C. guilliermondii being the most active. The effects of xylose depended on the yeast background and experimental conditions.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Biotechnology & Applied Microbiology
Tai Man Louie, Kailin Louie, Samuel DenHartog, Sridhar Gopishetty, Mani Subramanian, Mark Arnold, Shuvendu Das
Summary: The study introduced a two-stage strategy for producing bio-xylitol from D-xylose using recombinant Pichia pastoris expressing a heterologous xylose reductase gene. The biotransformation was shown to be efficient with high conversion rates, and the whole cells could be reused without loss of activity. This method can be applied to other oxidoreductase reactions, demonstrating its potential for various industrial applications beyond xylitol production.
MICROBIAL CELL FACTORIES
(2021)
Article
Agricultural Engineering
Saumya Singh, Dalveer Kaur, Sudesh Kumar Yadav, Meena Krishania
Summary: The study focuses on optimizing operational parameters for acid-catalyzed rice straw pretreatment in xylitol production. By pretreating rice straw soaked in 1.5% H2SO4 at 121 degrees C for 30 minutes, a xylose yield of 75.77% was achieved. Fermenting the hydrolysate with C. tropicalis MTCC 6192 resulted in a xylitol yield of 0.6 g/g of xylose, showing promise for commercial scale production and minimal pollution impact.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lihua Zhang, Zhen Chen, Junhua Wang, Wei Shen, Qi Li, Xianzhong Chen
Summary: Through a combination of metabolic engineering and process optimization, a uracil-deficient Candida tropicalis strain was successfully engineered to enhance xylitol production from xylose mother liquor (XML). The study provided a convenient method to transform the industrial by-product XML into the useful material xylitol.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Pattanan Songdech, Rawitsara Intasit, Yodying Yingchutrakul, Chutikarn Butkinaree, Khanok Ratanakhanokchai, Nitnipa Soontorngun
Summary: The transcription factor Znf1 plays a key role in activating cryptic xylose metabolism in yeast for xylitol production. Overexpression of ZNF1 and the absence of the xylose suppressor bud21 Delta can significantly increase xylitol production and improve tolerance to lignocellulosic inhibitors. This study highlights the potential application of Znf1 and Bud21 in enhancing xylose utilization for sustainable lignocellulose-based bioeconomy with a generally recognised as safe yeast.
MICROBIAL CELL FACTORIES
(2022)
Article
Biotechnology & Applied Microbiology
Thomas P. West
Summary: Xylitol is an industrially important chemical with commercial applications, particularly as a sweetener and in biomedicine. Candida species of yeast are able to synthesize xylitol, with xylitol reductase enzyme playing a key role in this process. The use of agricultural residues and grasses as hydrolysate substrates for yeast xylitol production can potentially reduce costs and increase production efficiency.
FERMENTATION-BASEL
(2021)
Article
Chemistry, Inorganic & Nuclear
Sho Yamaguchi, Tomoo Mizugaki, Takato Mitsudome
Summary: The highly efficient and selective hydrogenation of D-xylose to D-xylitol was achieved using a hydrotalcite-supported nickel phosphide nanoparticle catalyst. Notably, the reaction proceeded even under 1 bar of H-2 or at room temperature for the first time. The catalyst exhibited the highest activity among previously reported non-noble metal catalysts, with potential for industrial production of D-xylitol.
EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Viviani Tadioto, Leticia M. Milani, evelyn T. Barrilli, Cristina W. Baptista, Leticia Bohn, Aline Dresch, Ricardo Harakava, Odinei Fogolari, Guilherme M. Mibielli, Joao P. Bender, Helen Treichel, Boris U. Stambuk, Caroline Mueller, Sergio L. Alves
Summary: This study isolated and identified four new indigenous yeast strains and revealed their metabolic characteristics under different carbon sources and oxygen conditions through cultivation and fermentation experiments. The results are important for increasing knowledge about indigenous yeasts and demonstrating the biotechnological potential of these strains.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katarina Suchova, Andrej Chyba, Zuzana Hegyi, Martin Rebros, Vladimir Puchart
Summary: Xylanases are enzymes that break down hemicellulose in plant cell walls, with potential for biotechnological applications in lignocellulose product preparation. This study characterized a GH30 xylanase SlXyn30A from the yeast Sugiyamaella lignohabitans, expanding the group of glucuronoxylanases/xylobiohydrolases within GH30.
Article
Biotechnology & Applied Microbiology
Yixuan Zhu, Jingtao Zhang, Lang Zhu, Zefang Jia, Qi Li, Wei Xiao, Limin Cao
Summary: Efficient xylose fermentation in budding yeast was achieved through rational promoter elements engineering, with HXT7 showing the best performance among surveyed promoters. The redox balance of the xylose utilization pathway was optimized to achieve a balanced xylose metabolism toward ethanol formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Wencheng Yin, Yujin Cao, Miaomiao Jin, Mo Xian, Wei Liu
Summary: A biosynthetic pathway for the production of xylose from glucose in Escherichia coli was designed, leading to an increased yield of xylose to 3.3 g/L through pathway optimization. The biosynthetic pathway of xylose from glucose was found to be universally applicable for the synthesis of downstream compounds of xylose.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Food Science & Technology
Priscilla Macedo Lima Andrade, Luciana Baptista, Camila Oliveira Bezerra, Rosane Marina Peralta, Aristoteles Goes-Neto, Ana Paula Trovatti Uetanabaro, Andrea Miura da Costa
Summary: In this study, a newly isolated tannase enzyme from Penicillium rolfsii CCMB 714 was immobilized on calcium alginate and showed high efficiency and stability in clarifying juice, especially apple juice. The immobilized tannase demonstrated improved thermal and pH stability compared to the free form, making it a promising candidate for future industrial applications in juice processing.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2021)
Article
Energy & Fuels
Barbara Ribeiro Alves Alencar, Nilson Medeiros, Carolaine Larissa Lira da Silva, Aldo Torres, Emmanuel Damilano Dutra, Everardo Valadares de Sa Barreto Sampaio, Romulo Simoes Cezar Menezes, Marcos Antonio Morais Junior
Summary: This study evaluated the effects of harvest time and season on biomass pH and dry matter concentration of cactus pear cladodes and compared the hydrolysis and fermentation of fresh and dried biomass. The results showed that drying at 105 degrees C for 12 hours yielded the best results in terms of solids concentration. The enzymatic hydrolysis and fermentation of the dried biomass resulted in the production of glucose and ethanol.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biotechnology & Applied Microbiology
Irina Charlot Pena-Moreno, Denise Castro Parente, Karolini Miranda da Silva, Elton Pedro Nunes Pena, Fabiana Aparecida Cavalcante Silva, Tercilio Calsa Junior, Will de Barros Pita, Marcos Antonio de Morais Jr
Summary: The yeast Dekkera bruxellensis exhibits superior anaerobic growth and ethanol yield in the presence of nitrate, due to balanced activation of TORC1 and NCR de-repression mechanisms regulating cell metabolism. On the other hand, poor growth in aerobiosis is likely caused by oxidative stress triggered by nitrate when oxygen is present.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Weilan Gomes da Paixao Melo, Tassio Brito de Oliveira, Silvio Lovato Arcuri, Paula Benevides de Morais, Fernando Carlos Pagnocca
Summary: The study found that the external refuse dump in the nests of Acromyrmex balzani in the Brazilian savanna has richer and more diverse yeast communities compared to the fungus gardens. These yeasts are capable of degrading carbon polymer and utilizing carbon sources in plant materials, indicating that this interaction may vary depending on environmental conditions.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Hector M. C. Navarro, Ciro Ramon Felix, Victor D. F. S. Tavares, Francisca M. P. de Sousa, Ana R. O. Santos, Paula B. Morais, Carlos A. Rosa, Patricia Valente, Melissa Fontes Landell
Summary: In this study, eight yeast isolates closely related to the genus Tremella were obtained from bromeliads in different locations in Brazil. Sequence analysis revealed that they may represent two novel species of Tremella.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Matheus Mikio Takeyama, Marcia Correa de Carvalho, Helena Sacco Carvalho, Cristiane Rodrigues Silva, Ana Paula Trovatti Uetanabaro, Andrea Miura da Costa, Joseph A. Medeiros Evaristo, Fabio Cesar Sousa Nogueira, Ana Elizabeth Cavalcante Fai, Maria Gabriela Bello Koblitz
Summary: A sequential design strategy was used to optimize the secretion of pectinases by a Saccharomyces cerevisiae strain on passion fruit residue flour. The results showed that passion fruit residue flour might be a cost-effective and eco-friendly substrate for pectinase production.
Article
Microbiology
Junia Schultz, Mariana Teixeira Dornelles Parise, Doglas Parise, Laenne G. Medeiros, Thiago J. Sousa, Rodrigo B. Kato, Ana Paula Trovatti Uetanabaro, Fabricio Araujo, Rommel Thiago Juca Ramos, Siomar de Castro Soares, Bertram Brenig, Vasco Ariston de Carvalho Azevedo, Aristoteles Goes-Neto, Alexandre S. Rosado
Summary: This study provides insights into the genomic features and phylogenomics of Anoxybacillus strains inhabiting the Antarctic thermophilic ecosystem. The strains showed high genomic similarity to each other and to A. flavithermus, and possessed various genes related to resistance, metabolism, and temperature adaptation. Moreover, prediction of glycosyl hydrolase enzyme-encoding genes, secondary metabolites, and prophage sequences suggests their potential for biotechnological applications.
Article
Biodiversity Conservation
Guilherme Sena, Alan M. Tonin, Adriano Caliman, Marcos Callisto, Neusa Hamada, Luiz U. Hepp, Vania L. Kowalczuk, Renato T. Martins, Adriana O. Medeiros, Paula B. Morais, Marcelo Moretti, Yara Moretto, Mauricio M. Petrucio, Lais Salgueiro, Luciana S. Carneiro, Gisele M. dos Santos, Edson S. A. Junior, Lorrane A. M. Feitoza, Jose F. Goncalves Jr
Summary: Riparian forests provide plant litter for terrestrial and aquatic ecosystems, and this study explored the traits of litter from riparian forests in three South American biomes. The findings showed similarities and differences in litter traits, with litter from drier biomes being more chemically defended and lower in nutritional quality than litter from humid biomes. The study also found that trait differences across biomes were primarily determined by environmental factors rather than phylogenetics.
Review
Microbiology
Allyson Andrade Mendonca, Walter de Paula Pinto-Neto, Giselle Alves da Paixao, Dayane da Silva Santos, Marcos Antonio De Morais Jr, Rafael Barros De Souza
Summary: This review provides an overview of the technological and biological challenges in the production and use of probiotic bacteria for promoting human health. It discusses the challenges in industrial production, from physiology to stress resistance, and emphasizes the importance of maintaining cell viability and vitality in the product. It also highlights the obstacles probiotic cells face within the human body, such as enzymes, antibacterial molecules, and pH changes.
Article
Biochemistry & Molecular Biology
Gilberto Henrique Teles, Mariana Rodrigues Xavier, Jackeline Maria Da Silva, Rafael Barros De Souza, Will de Barros Pita, Marcos Antonio de Morais Jr
Summary: Dekkera bruxellensis is an important yeast in industrial fermentation processes. Acetate, a metabolite found in its cultivations, affects its fermentation capacity. This study investigated the role of acetate metabolism in respiring cells using different nitrogen sources. The results showed that acetate production is crucial for carbon assimilation and provided valuable insights into the oxidative metabolism of this yeast.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Carlos A. Rosa, Marc-Andre Lachance, Savitree Limtong, Ana R. O. Santos, Mellisa F. Landell, Andreas K. Gombert, Paula B. Morais, Jose P. Sampaio, Carla Goncalves, Paula Goncalves, Aristoteles Goes-Neto, Rosangela Santa-Brigida, Marlucia B. Martins, Daniel H. Janzen, Winnie Hallwachs
Summary: Tropical rainforests and related biomes are tremendous sources of novel yeasts. Tropical yeasts participate in several ecological interactions that contribute to the health of these ecosystems. Most studies of yeast diversity in tropical forests were carried out using culture-dependent methods. Yeasts from tropical rainforests are rich sources for bioinnovation.
Article
Microbiology
Barbara Ribeiro Alves Alencar, Renan Anderson Alves de Freitas, Victor Emanuel Petricio Guimaraes, Rayssa Karla Silva, Carolina Elsztein, Suzyanne Porfirio da Silva, Emmanuel Damilano Dutra, Marcos Antonio de Morais Junior, Rafael Barros de Souza
Summary: In this study, a yeast strain called Meyerozyma caribbica URM 8365 was isolated and tested from soil irrigated with vinasse, which has high mineral content and acidic pH. The strain was able to ferment glucose and use xylose when oxygen was provided. It also showed tolerance to inhibitors and high ethanol production efficiencies in various substrates. A proposed metabolic model allowed for the calculation of carbon allocation for ethanol and/or xylitol production and could be used to design an industrial plant for combined production using different substrates.
Article
Biotechnology & Applied Microbiology
Rayssa Karla Silva, Will de Barros Pita, Marcos Antonio de Morais, Rafael Barros de Souza
Summary: This study investigated the detrimental effect of excessive minerals in industrial substrates on the fermentation performance of Saccharomyces cerevisiae and its effect on the physiology of Dekkera bruxellensis. The highest mineral toxicity was observed with Cu2+, which depended on the level of medium aeration. Copper stimulated respiration and glucose fermentation was hindered by most growth inhibitors, leading to changes in carbon distribution. The negative effect of Cu2+ on yeast fermentation was partially alleviated by Mg2+ and Mn2+, similar to magnesium antagonism observed in S. cerevisiae.
LETTERS IN APPLIED MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Mariana Rodrigues Xavier, Gilberto Henrique Teles, Jennyfer Martins de Carvalho, Leucio Duarte Vieira, Will de Barros Pita, Marcos Antonio de Morais
Summary: This study aimed to investigate the biological defence mechanism used by the yeast Dekkera bruxellensis to tolerate the presence of exogenous H2O2. The combination of glutathione peroxidase and sulfhydryl-containing proteins was found to be the preferred defence mechanism against H2O2, especially under respiratory metabolism. However, the action of this mechanism was suppressed when the cells were metabolizing nitrate.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Taides T. dos Santos, Ana Maria O. Pes, Paula B. de Morais
Summary: Research on the fungal community associated with the digestive tract of aquatic shredder larvae revealed that the fungi primarily derive from food sources in riparian vegetation or indigenous to aquatic ecosystems. The diversity of fungal taxa is high, with a significant portion exhibiting cellulolytic activity and showing host specificity.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2021)
