Journal
ACS SYNTHETIC BIOLOGY
Volume 8, Issue 6, Pages 1224-1230Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00435
Keywords
artificial cell; protein membrane interactions; cell-free expression; membrane logic gate
Categories
Funding
- Northwestern's Biomedical Engineering Department
- National Institute of General Medical Sciences [T32GM008382]
- Searle Funds at The Chicago Community Trust
- National Science Foundation [1844336]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1844336] Funding Source: National Science Foundation
Ask authors/readers for more resources
The assembly of channel proteins into vesicle membranes is a useful strategy to control activities of vesicle-based systems. Here, we developed a membrane AND gate that responds to both a fatty acid and a pore-forming channel protein to induce the release of encapsulated cargo. We explored how membrane composition affects the functional assembly of alpha-hemolysin into phospholipid vesicles as a function of oleic acid content and alpha-hemolysin concentration. We then showed that we could induce alpha-hemolysin assembly when we added oleic acid micelles to a specific composition of phospholipid vesicles. Finally, we demonstrated that our membrane AND gate could be coupled to a gene expression system. Our study provides a new method to control the temporal dynamics of vesicle permeability by controlling when the functional assembly of a channel protein into synthetic vesicles occurs. Furthermore, a membrane AND gate that utilizes membrane-associating biomolecules introduces a new way to implement Boolean logic that should complement genetic logic circuits and ultimately enhance the capabilities of artificial cellular systems.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available