Journal
NUTRIENTS
Volume 11, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/nu11051116
Keywords
breast cancer; cancer cell migration; Ganoderma lucidum; lamellipodin; Rac
Categories
Funding
- National Institutes of Health [SC3GM111171, MD007583, GM103475]
- INBRE-Supplies for Graduate Students
- Puerto Rico Science, Technology and Research Trust (PRSTRT) [SGRP 2017-043]
- U.S. Dept. of Education Title-V-PPOHA [P031M105050]
- U.S. Dept. of Education Title-V-Cooperative [P031S130068]
- PRSTRT Hurricane Relief Funds
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Breast cancer (BC) is the second leading cause of cancer death among women worldwide. The main cause of BC morbidity and mortality is the invasiveness capacity of cancer cells that may lead to metastasis. Here, we aimed to investigate the therapeutic efficacy of Ganoderma lucidum extract (GLE)a medicinal mushroom with anticancer propertieson BC motility via the Rac/Lamellipodin pathway. GLE treatment effects were tested on MDA-MB-231 breast cancer cells. The effects were tested on cell viability, migration and invasion. Pulldowns, immunoblotting, and immunofluorescence were used to measure Rac activity and the expression of proteins involved in cell migration and in lamellipodia formation, respectively. As a result, GLE suppressed BC cell viability, migration, and invasion capacity. GLE impaired Rac activity, as well as downregulated Lamellipodin, ENA/VASP, p-FAK (Tyr925), Cdc42, and c-Myc expression. Lamellipodia formation was significantly reduced by GLE. In conclusion, we demonstrate that GLE reduces Rac activity and downregulates signaling molecules involved in lamellipodia formation. These novel findings serve as basis for further studies to elucidate the potential of GLE as a therapeutic agent regulating the Rac/Lamellipodin pathway in BC metastasis.
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