Article
Gastroenterology & Hepatology
Jyotirmayee Dey, Soumya Ranjan Mahapatra, T. Kiran Raj, Taranjeet Kaur, Parul Jain, Arushi Tiwari, Shubhransu Patro, Namrata Misra, Mrutyunjay Suar
Summary: This study aimed to design a novel vaccine against Enterococcus faecium using immunoinformatics approaches. Through in-depth analysis, suitable epitopes were selected for vaccine development. The constructed vaccine showed high immunogenicity and stability. Immune simulation results indicated a strong immune response, making it a promising target.
Article
Microbiology
Ana R. Freitas, Ana P. Tedim, Ana C. Almeida-Santos, Barbara Duarte, Houyem Elghaieb, Mohamed S. Abbassi, Abdennaceur Hassen, Carla Novais, Luisa Peixe
Summary: Through genomic analysis, researchers identified multidrug-resistant Enterococcus faecium strains causing human infections in Tunisian healthcare institutions. These strains were compared with non-human sources within the same country, revealing their spread across different hosts and regions with similar antibiotic resistance and virulence genetic patterns.
Article
Microbiology
Jaclyn A. Cusumano, Ruhma Khan, Zeel Shah, Cassie Philogene, Amrita Harrichand, Vanthida Huang
Summary: Penicillin plus ceftriaxone demonstrates similar synergistic activity against Enterococcus faecalis to ampicillin plus ceftriaxone. Isolates with a penicillin MIC of 4 mg/L and a ceftriaxone MIC of 512 or higher lack penicillin plus ceftriaxone synergy despite the penicillin susceptibility MIC breakpoint of 8 mg/L.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Gregory S. Canfield, Anushila Chatterjee, Juliel Espinosa, Mihnea R. Mangalea, Emma K. Sheriff, Micah Keidan, Sara W. McBride, Bruce D. McCollister, Howard C. Hang, Breck A. Duerkop
Summary: Enterococcus faecium, a commensal of the human intestine, has evolved into a hospital-adapted, multidrug-resistant pathogen. Bacteriophages, natural predators of bacteria, show potential as therapeutics against MDR E. faecium infections, despite the unknown molecular events governing their interactions. While phage resistance may emerge, it is shown that lytic phages could effectively synergize with antibiotics to slow or inhibit E. faecium growth.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Nicole E. Vanzeeland, Kathryn M. Schultz, Candice S. Klug, Christopher J. Kristich
Summary: Enterococci are normal human commensals that often cause hospital-acquired infections. They exhibit antibiotic resistance, including resistance to cephalosporins. A study on Enterococcus faecalis revealed that the transmembrane kinase IreK plays a crucial role in cephalosporin resistance, with its activity being boosted by the cytoplasmic protein GpsB. Phosphorylation of specific sites on GpsB negatively regulates IreK activity, while phosphorylation at a unique site enables efficient phosphorylation at other sites and modulates cephalosporin resistance.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Microbiology
Lara Thieme, Simon Briggs, Eamon Duffy, Oliwia Makarewicz, Mathias W. Pletz
Summary: This study suggests that benzylpenicillin/ceftriaxone is suitable for the treatment of E. faecalis infections with ceftriaxone MIC ≤ 256mg/L, but not for those with ceftriaxone MIC ≥ 512mg/L. Further investigation is needed to analyze the relationship between ceftriaxone susceptibility and penicillin/ceftriaxone synergy.
Article
Microbiology
Helene Faury, Ronan Le Guen, Vanessa Demontant, Christophe Rodriguez, Berenice Souhail, Adrien Galy, Sarah Jolivet, Raphael Lepeule, Jean-Winoc Decousser, Vincent Fihman, Paul-Louis Woerther, Guilhem Royer
Summary: This study provides important new insight into the clinical features and clades associated with ampicillin-susceptible and ampicillin-resistant Enterococcus faecium isolates. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with a custom-built database may enable the differentiation of different clades, facilitating the management of these infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biology
Yothin Teethaisong, Piyasiri Chueakwon, Kulwara Poolpol, Intu-orn Ayamuang, Siriporn Suknasang, Chittadech Apinundecha, Griangsak Eumkeb
Summary: This study investigated the antibacterial potential of Stephania suberosa extract (SSE) and its synergism with ampicillin (AMP) or vancomycin (VAN) against AMP- and VAN-resistant Enterococcus faecium. The results showed that SSE inhibited the growth of E. faecium and exhibited synergistic interaction with AMP and VAN. These findings support that SSE could reverse the activity of AMP and VAN, and synergistically inhibit AMP- and VAN-resistant E. faecium.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2023)
Article
Microbiology
Kavindra Singh, Maria Camila Montealegre, Barbara E. Murray
Summary: In previous research, it was found that health care-associated Glade A1 strains of Enterococcus faecium were outcompeted by Glade B strains in murine gastrointestinal colonization. However, parenterally administered piperacillin-tazobactam and ceftriaxone significantly promoted the colonization of Glade A1 strains, except for the least beta-lactam-resistant A1 strain. This study reflects the advantage of beta-lactam administration in promoting the colonization of highly ampicillin-resistant E. faecium strains over susceptible strains, similar to what happens in hospitalized patients.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Jaclyn A. Cusumano, Kathryn E. Daffinee, Paul Ugalde-Silva, Wolfgang Peti, Michel Arthur, Charlene Desbonnet, Louis B. Rice, Kerry L. LaPlante, Monica Garcia-Solache
Summary: Ampicillin-ceftriaxone is an effective treatment for Enterococcus faecalis endocarditis. This study characterized the penicillin-binding protein (PBP) profiles of different E. faecalis strains and tested for synergy to optimize beta-lactam treatment options for E. faecalis infections. Increased expression of PBP4 and PBP2B was observed in a strain with elevated MICs, but PBP2B did not significantly contribute to the elevated beta-lactam MICs. The combination of meropenem plus ceftriaxone or ertapenem plus ceftriaxone showed the most consistent synergistic activity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Helene Faury, Ronan Le Guen, Vanessa Demontant, Christophe Rodriguez, Berenice Souhail, Adrien Galy, Sarah Jolivet, Raphael Lepeule, Jean-Winoc Decousser, Vincent Fihman, Paul-Louis Woerther, Guilhem Royer
Summary: This study provides important insights into the clinical features and clades associated with ampicillin-susceptible and ampicillin-resistant Enterococcus faecium isolates. The findings suggest that ampicillin-resistant isolates are associated with a longer hospital stay, history of prior hospitalization, and the carriage of multidrug-resistant organisms. Furthermore, the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry with a custom-built database may facilitate the management of these infections by distinguishing between clade B, which is typically susceptible to ampicillin, and clade A1/A2, which are typically resistant.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Mina Nasiri, Shahram Hanifian
Summary: The study assessed the prevalence of enterococci in pasteurized milk samples from retail stores in Northwest Iran, finding that most isolates were identified as E. faecalis or E. faecium, carrying various virulence genes and showing high resistance to most antibiotics tested. Additionally, the isolates were able to produce biofilm, suggesting that pasteurized milk could potentially transmit multidrug-resistant virulent enterococci strains to humans.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Virology
S. Rigvava, I Kusradze, I. Tchgkonia, N. Karumidze, T. Dvalidze, M. Goderdzishvili
Summary: Enterococcus spp. is a common commensal microorganism, but some strains can cause opportunistic infections in humans. The spread of multi-resistant bacterial strains has renewed interest in phage therapy as a potential treatment for Enterococcus faecium infections.
Article
Food Science & Technology
Marlena Golas-Pradzynska, Magdalena Luszczynska, Jolanta Grazyna Rola
Summary: This study investigates the antimicrobial resistance, virulence, and resistance genes of Enterococcus faecalis and Enterococcus faecium isolated from raw goat's and sheep's milk and cheese. The results show that E. faecalis is the dominant species, with high levels of antimicrobial resistance and presence of virulence genes in some strains.
Article
Microbiology
Georgios V. Zacharopoulos, Georgios A. Manios, Marios Papadakis, Dimitra Koumaki, Sofia Maraki, Dimitrios Kassotakis, Eelco De Bree, Andreas Manios
Summary: This study evaluated the antibiotic resistance of E. faecalis in surgical patients and found an increasing incidence of E. faecalis infections in the clinic. The results showed that ampicillin and teicoplanin had similar efficacy in treating E. faecalis infections, suggesting that ampicillin could be used as effective empiric therapy.
Article
Immunology
Maria Camila Montealegre, Kavindra V. Singh, Barbara E. Murray
JOURNAL OF INFECTIOUS DISEASES
(2016)
Article
Microbiology
Maria Camila Montealegre, Sabina Leanti La Rosa, Jung Hyeob Roh, Barrett R. Harvey, Barbara E. Murray
Article
Immunology
Maria Camila Montealegre, Kavindra V. Singh, Sudha R. Somarajan, Puja Yadav, Chungyu Chang, Robert Spencer, Jouko Sillanpaa, Hung Ton-That, Barbara E. Murray
INFECTION AND IMMUNITY
(2016)
Article
Microbiology
Sabina Leanti La Rosa, Maria Camila Montealegre, Kavindra V. Singh, Barbara E. Murray
Article
Multidisciplinary Sciences
Emily K. Stinemetz, Peng Gao, Kenneth L. Pinkston, Maria Camila Montealegre, Barbara E. Murray, Barrett R. Harvey
Article
Public, Environmental & Occupational Health
Tala Navab-Daneshmand, Max N. D. Friedrich, Marja Gachter, Maria Camila Montealegre, Linn S. Mlambo, Tamuka Nhiwatiwa, Hans-Joachim Mosler, Timothy R. Julian
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2018)
Article
Microbiology
Maria Camila Montealegre, Juan Jose Maya, Adriana Correa, Paula Espinal, Maria F. Mojica, Sory J. Ruiz, Fernando Rosso, Jordi Vila, John P. Quinn, Maria Virginia Villegas
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2012)
Letter
Microbiology
L. J. Rojas, M. F. Mojica, V. M. Blanco, A. Correa, M. C. Montealegre, E. De La Cadena, J. J. Maya, R. D. Camargo, J. P. Quinn, M. V. Villegas
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2013)
Letter
Microbiology
A. Correa, M. C. Montealegre, M. F. Mojica, J. J. Maya, L. J. Rojas, E. P. De La Cadena, S. J. Ruiz, M. Recalde, F. Rosso, J. P. Quinn, M. V. Villegas
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2012)
Article
Microbiology
Ester Pietta, Maria Camila Montealegre, Jung Hyeob Roh, Pier Sandro Cocconcelli, Barbara E. Murray
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2014)
Letter
Infectious Diseases
Maria F. Mojica, Adriana Correa, Deninson A. Vargas, Juan J. Maya, Maria C. Montealegre, Laura J. Rojas, Sory J. Ruiz, John P. Quinn, Maria V. Villegas
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2012)
Article
Multidisciplinary Sciences
Jouko Sillanpaeae, Chungyu Chang, Kavindra V. Singh, Maria Camila Montealegre, Sreedhar R. Nallapareddy, Barrett R. Harvey, Hung Ton-That, Barbara E. Murray
Article
Genetics & Heredity
Alexandra N. Marshall, Maria Camila Montealegre, Claudia Jimenez-Lopez, Michael C. Lorenz, Ambro van Hoof
Article
Biotechnology & Applied Microbiology
Maria Camila Montealegre, Subarna Roy, Franziska Boni, Muhammed Iqbal Hossain, Tala Navab-Daneshmand, Lea Caduff, A. S. G. Faruque, Mohammad Aminul Islam, Timothy R. Julian
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)