Article
Biotechnology & Applied Microbiology
Ahmed M. Soliman, Hirofumi Nariya, Daiki Tanaka, Liansheng Yu, Junzo Hisatsune, Shizuo Kayama, Kohei Kondo, Motoyuki Sugai, Toshi Shimamoto, Tadashi Shimamoto
Summary: This study characterized carbapenemase-producing Klebsiella pneumoniae and Acinetobacter baumannii isolated from fresh vegetables in Japan. The findings revealed the presence of various antimicrobial resistance genes in these bacteria, posing a potential threat to food safety and public health.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Infectious Diseases
Hasan Ejaz, Mahtab Ahmad, Sonia Younas, Kashaf Junaid, Khalid Omer Abdalla Abosalif, Abualgasim Elgaili Abdalla, Ayman Ali Mohammed Alameen, Mohammed Yagoub Mohammed Elamir, Syed Nasir Abbas Bukhari, Naveed Ahmad, Muhammad Usman Qamar
Summary: The study revealed the widespread distribution of CR-AB ST2 clone harboring bla(OXA-23) and bla(NDM-1) in clinical settings in Pakistan, potentially increasing mortality rates. Strict compliance with the National Action Plan on Antimicrobial Resistance is necessary to mitigate the impacts of these strains.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Julia Moreno-Manjon, Santiago Castillo-Ramirez, Keith A. Jolley, Martin C. J. Maiden, Catalina Gayosso-Vazquez, Jose Luis Fernandez-Vazquez, Valeria Mateo-Estrada, Silvia Giono-Cerezo, Maria Dolores Alcantar-Curiel
Summary: This study analyzes the characteristics and related genes of A. baumannii isolates causing HAIs and outbreaks in a Mexican hospital over ten years. The isolates mainly belong to the global international clone 2 and the Latin America endemic clone 5, and they are extensively drug-resistant. Carbapenem resistance is commonly associated with oxacillinases, and significant biofilm-related genes were identified.
Article
Microbiology
Carina Mueller, Sandra Reuter, Julia Wille, Kyriaki Xanthopoulou, Danuta Stefanik, Hajo Grundmann, Paul G. Higgins, Harald Seifert
Summary: This study conducted molecular epidemiology and global distribution research on 313 carbapenem-resistant Acinetobacter baumannii isolated from 114 study centers in 47 countries. The results revealed a wide variation in the distribution of carbapenemase encoding genes among different geographical regions.
Article
Microbiology
Mohammad Al-Tamimi, Hadeel Albalawi, Mohamd Alkhawaldeh, Abdullah Alazzam, Hassan Ramadan, Majd Altalalwah, Ahmad Alma'aitah, Dua'a Al Balawi, Sharif Shalabi, Jumana Abu-Raideh, Ashraf Khasawneh, Farah Alhaj, Kamal Hijawi
Summary: This study is the first large-scale, multicenter, and prolonged research on Acinetobacter baumannii infections in Jordan, providing insights into the epidemiology and molecular characteristics of MDR-A. baumannii in the country. Attention to high-risk patients and early identification are crucial. Colistin and tigecycline are the most effective antimicrobials.
Article
Microbiology
Zixuan Ding, Zhaoyinqian Li, Yuanqing Zhao, Jingchen Hao, Tingting Li, Yao Liu, Zhangrui Zeng, Jinbo Liu
Summary: In this study, we isolated and characterized a tigecycline-resistant Acinetobacter pittii clinical strain carrying multiple antibiotic resistance genes. Whole-genome investigation revealed horizontal transfer of these resistance genes, including the discovery of two novel bla(OXA) genes, bla(OXA-1045) and bla(OXA-213). The strain exhibited differences in chromosomal composition and biofilm formation. Additionally, the expression levels of RND efflux pump-related genes were found to be correlated with tigecycline resistance.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Katerina Tsilipounidaki, Christos-George Gkountinoudis, Zoi Florou, George C. Fthenakis, Vivi Miriagou, Efthymia Petinaki
Summary: The present study reports the detection and molecular characterization of nine blaNDM-1-positive Acinetobacter baumannii isolates isolated from patients in a tertiary care hospital in Central Greece. The isolates were characterized using whole genome sequencing, revealing their multilocus sequencing typing (MLST), bla(NDM-1) environment, resistome, and virulence genes. The results showed that the isolates belonged to four different clones. This is the first report of the emergence of polyclonal bla(NDM-1)-positive Acinetobacter baumannii in Greece. The findings highlight the need for diligent surveillance protocols to limit the horizontal transfer of the bla(NDM-1) gene.
Article
Public, Environmental & Occupational Health
Chia-Hung Tsai, Nan-Yao Lee, Chien-Ming Chao, Chi-Chung Chen, Chih-Cheng Lai, Chung-Han Ho, Yu-Chin Chen, Po-Yu Chen, Hung-Jen Tang, Hung-Jui Chen, Bo-An Su, Hsin-Yu Lai, Julie Chi Chow, Tu-Hsuan Chang
Summary: This study investigated the characteristics of blaNDM-producing Enterobacterales in southern Taiwan, revealing an increase in blaNDM-5 and blaOXA-181 co-harboring strains, particularly the dissemination of E. coli ST8346. These strains exhibited high-level resistance to carbapenems and aminoglycosides, and a distinct IncF plasmid encoding blaNDM-5 was identified.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Infectious Diseases
Bence Balazs, Zoltan Toth, Fruzsina Nagy, Renato Kovacs, Hajnalka Toth, Jozsef Balint Nagy, Akos Toth, Krisztina Szarka, Laszlo Majoros, Gabor Kardos
Summary: The dominant carbapenem resistant Acinetobacter baumannii carrying bla(OXA-23-like) carbapenemase was replaced by strains carrying bla(OXA-40-like) in a Hungarian tertiary-care center with high meropenem but relatively low imipenem usage. Alterations in antibiotic consumption, particularly the uniform usage of meropenem, may have contributed to this strain replacement. Time-kill assays showed significant differences in susceptibility to meropenem between different sequence types, but not in imipenem resistance.
Article
Infectious Diseases
Yuiko Takebayashi, Jacqueline Findlay, Kate J. Heesom, Philip J. Warburton, Matthew B. Avison, Benjamin A. Evans
Summary: Elevated carbapenem MICs were observed by expression of OxaAb variants carrying clinically prevalent substitutions I129L and L167V, requiring overexpression by the upstream ISAba1 promoter. LC-MS/MS analysis confirmed overexpression of these variants, with no other differences in expression levels of proteins commonly associated with carbapenem resistance detected.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Immunology
Sandra Sanchez-Urtaza, Alain Ocampo-Sosa, Ainhoa Molins-Bengoetxea, Mohammed A. A. El-Kholy, Marta Hernandez, David Abad, Sherine M. M. Shawky, Itziar Alkorta, Lucia Gallego
Summary: This study investigated the genetic characteristics and antimicrobial susceptibility profile of multidrug resistant Acinetobacter baumannii clinical isolates obtained from hospitals in Alexandria, Egypt. The results showed resistance to multiple antibiotics and the presence of various antibiotic resistance genes and virulence factors. The study highlights the importance of investigating molecular epidemiology to control the spread of these multidrug resistant isolates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Swarnaletchumi Kanapathy, Godwin Attah Obande, Candy Chuah, Rafidah Hanim Shueb, Chan Yean Yean, Kirnpal Kaur Banga Singh
Summary: An enzyme-based electrochemical genosensor was developed for rapid detection of A. baumannii infections. With the analysis of asymmetric PCR amplicons of the bla(OXA-51-like) gene, the genosensor exhibited 100% specificity and sensitivity, and demonstrated good stability under different storage conditions.
Article
Biotechnology & Applied Microbiology
Hye-Rim Kim, Yong-Bin Eom
Summary: This study demonstrates the potential of repurposing auranofin as an antibiotic adjuvant against carbapenemase-producing Acinetobacter baumannii. The synergistic effect of auranofin and doripenem is confirmed, as well as the inhibitory effects of auranofin on biofilms and motility. Auranofin also induces changes in gene expression related to carbapenem resistance.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Infectious Diseases
Haiyang Liu, Robert A. Moran, Ying Chen, Emma L. Doughty, Xiaoting Hua, Yan Jiang, Qingye Xu, Linghong Zhang, Jessica M. A. Blair, Alan McNally, Willem van Schaik, Yunsong Yu
Summary: A novel plasmid type, GR34, carrying multiple antibiotic resistance genes was identified in this study. These plasmids are found in various Acinetobacter species in diverse environments, displaying considerable variation in accessory content.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Review
Infectious Diseases
Filippo Medioli, Erica Bacca, Matteo Faltoni, Giulia Jole Burastero, Sara Volpi, Marianna Menozzi, Gabriella Orlando, Andrea Bedini, Erica Franceschini, Cristina Mussini, Marianna Meschiari
Summary: Despite global efforts to control the spread of carbapenem-resistant Acinetobacter baumannii (CRAB), it remains a significant threat with high mortality rates. This review aims to provide evidence on the best infection prevention and control strategies to combat CRAB in endemic hospitals. The study found that while effective measures were implemented during outbreaks, the reported IPC measures varied greatly and were dependent on the setting and structural characteristics of the wards.
Article
Virology
Philippe Colson, Philippe Gautret, Jeremy Delerce, Herve Chaudet, Pierre Pontarotti, Patrick Forterre, Raphael Tola, Marielle Bedotto, Lea Delorme, Wahiba Bader, Anthony Levasseur, Jean-Christophe Lagier, Matthieu Million, Nouara Yahi, Jacques Fantini, Bernard La Scola, Pierre-Edouard Fournier, Didier Raoult
Summary: This study investigated the dynamics of the French variant Marseille-4 of SARS-CoV-2 over a 10-month period, identifying two subvariants and analyzing their mutations and lifetimes. The findings suggest that beneficial mutations can initiate new outbreaks, while the accumulation of other mutations can result in the disappearance of lineages.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Multidisciplinary Sciences
Samara Mejri, Alina Ghinet, Maryline Magnin-Robert, Beatrice Randoux, Cristina-Maria Abuhaie, Benoit Tisserant, Philippe Gautret, Benoit Rigo, Patrice Halama, Philippe Reignault, Ali Siah
Summary: The current global focus on agroecology and green agriculture requires the discovery of new ecofriendly and sustainable plant protection tools. This study synthesized 30 molecules from pyroglutamic acid, sourced from sugar beet byproducts, and examined their biological activity on wheat-Zymoseptoria tritici. The molecules provided significant disease protection when applied to the leaves, with up to 63% reduction in disease severity. Further investigation revealed that their activity was dependent on the activation of plant defense mechanisms. This study introduces a new family of bio-sourced resistance inducers and contributes to the development of sustainable agriculture.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Sory Ibrahima Traore, Cheikh Ibrahima Lo, Maaloum Mossaab, Guillaume Durand, Jean Christophe Lagier, Didier Raoult, Pierre-Edouard Fournier, Florence Fenollar
Summary: Bacterial strain Marseille-P3954 was isolated from a stool sample of a 35-year-old male patient living in France. It belongs to a new bacterial genus, classified in a new family, based on its genomic, phenotypic, and phylogenetic evidences. The proposed classification is Maliibacterium massiliense gen. nov., sp. nov. with the type strain Marseille-P3954 (CSUR P3954 = CECT 9568).
CURRENT MICROBIOLOGY
(2023)
Article
Infectious Diseases
Khouloud Slimene, Allaaeddin El Salabi, Olfa Dziri, Najla Mathlouthi, Seydina M. Diene, Elhussan Ahmed Mohamed, Jadalla M. A. Amhalhal, Mohammed O. Aboalgasem, Jomaa F. Alrjael, Jean-Marc Rolain, Chedly Chouchani
Summary: Antimicrobial resistance, especially to carbapenems, poses a major threat to public health. A study conducted in Ibn Sina Hospital in Libya identified low susceptibility to carbapenems among Gram-negative bacteria. Molecular investigations revealed the presence of New Delhi metallo-beta-lactamases-1 and Verona integron-encoded metallo-beta-lactamase enzymes in Pseudomonas spp. Additionally, a new gene and resistance gene were discovered in Acinetobacter baumannii.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Microbiology
Sarrah Landolsi, Rachid Selmi, Linda Hadjadj, Asma Ben Haj Yahia, Lilia Messadi, Jean-Marc Rolain
Summary: A study conducted in Tunisia found that rodents can potentially transmit multidrug resistant bacteria, posing a public health problem. The researchers isolated Enterobacteriaceae strains from the intestines of rats captured from various locations and found that rodents may play a role in the spread of antimicrobial resistant E. coli.
VETERINARY MICROBIOLOGY
(2023)
Correction
Genetics & Heredity
Sahare Kokcha, Ajay Kumar Mishra, Jean-Christophe Lagier, Matthieu Million, Quentin Leroy, Didier Raoult, Pierre-Edouard Fournier
ENVIRONMENTAL MICROBIOME
(2023)
Letter
Infectious Diseases
Hubert Bassene, Masse Sambou, Marielle Bedetto, Philippe Colson, Oleg Mediannikov, Ndiaw Goumballa, Georges Diatta, Philippe Gautret, Florence Fenollar, Cheikh Sokhna
NEW MICROBES AND NEW INFECTIONS
(2023)
Correction
Infectious Diseases
Philippe Gautret, Linda Houhamdi, Nhu Ngoc Nguyen, Van Thuan Hoang, Audrey Giraud-Gatineau, Didier Raoult
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Anna Barbiero, Tommaso Manciulli, Michele Spinicci, Iacopo Vellere, Maria Grazia Colao, Gian Maria Rossolini, Alessandro Bartoloni, Didier Raoult, Lorenzo Zammarchi
Summary: This study reports ten cases of SENLAT in Tuscany, Italy, with symptoms including scalp eschar and cervical lymphadenopathy. The difficulty of microbiological diagnosis and previous antibiotic treatment administration hindered the identification of the causative agent.
Expression of Concern
Immunology
S. Meconi, C. Capo, M. Remacle-Bonnet, G. Pommier, D. Raoult, J. L. Mege
INFECTION AND IMMUNITY
(2023)
Correction
Public, Environmental & Occupational Health
Thi Loi Dao, Van Thuan Hoang, Amal Magmoun, Tran Duc Anh Ly, Sophie Alexandra Baron, Linda Hadjadj, Naomie Canard, Tassadit Drali, Frederique Gouriet, Didier Raoult, Philippe Parola, Pierre Marty, Jean-Marc Rolain, Philippe Gautret
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Review
Cell Biology
Seydina M. Diene, Pierre Pontarotti, Said Azza, Nicholas Armstrong, Lucile Pinault, Eric Chabriere, Philippe Colson, Jean-Marc Rolain, Didier Raoult
Summary: Beta-lactamase enzymes are capable of conferring resistance to widely used antibiotics in human medicine, making them a topic of significant interest. Among these enzymes, the class B beta-lactamases are a type of metallo-beta-lactamase fold protein found not only in bacteria but also in other organisms. Previously unexplored in other organisms due to various barriers, modern search approaches have now uncovered their presence in different organisms including Bacteria, Archaea, Nanoarchaeota, Asgard, Humans, Giant viruses, and Candidate Phyla Radiation (CPR). These multifunctional enzymes exhibit diverse enzymatic or non-enzymatic activities, with at least thirteen reported activities such as beta-lactamase, ribonuclease, nuclease, glyoxalase, lactonase, phytase, ascorbic acid degradation, anti-cancer drug degradation, or membrane transport. This review discusses the existence of M beta L fold enzymes in different domains of life, presents suitable approaches for studying their homologous sequences in unexpected sources, and presents described M beta L fold enzymes with demonstrated enzymatic or non-enzymatic activities.
Article
Microbiology
Fatou Samba Diouf, Mamadou Beye, Mapenda Gaye, Babacar Mbaye, Stephane Alibar, Mariema Sarr, Gregory Dubourg, Jean-Christophe Lagier, Cheikh Sokhna, Florence Fenollar, Pierre-Edouard Fournier, Cheikh Ibrahima Lo
Summary: Blood is normally sterile, but bacteria in the bloodstream indicate an anomaly. By performing a blood culture on a patient with thalassemia major, a new strain of Streptococcus was isolated and identified through phenotypic, phylogenetic, and genomic characteristics analysis. Named Streptococcus thalassemiae sp. nov., this strain showed similarity to Streptococcus mitis but had significant differences based on DNA-DNA hybridization and average nucleotide identity analysis. This research demonstrates the importance of studying bacterial strains in blood cultures for accurate diagnosis.
INTERNATIONAL JOURNAL OF MICROBIOLOGY
(2023)
Letter
Infectious Diseases
Ali H. Hajeer, Hana M. A. Fakhoury, Tariq Z. Memish, Rana F. Kattan, Ziad A. Memish
NEW MICROBES AND NEW INFECTIONS
(2023)