Review
Oncology
Anna Kaluza, Justyna Szczykutowicz, Miroslawa Ferens-Sieczkowska
Summary: Aberrant protein glycosylation is a well-known hallmark of cancer, which can distinguish between benign prostate disease and cancer, as well as different stages of prostate cancer development and aggressiveness. Studies have shown that using glycosylation patterns as diagnostic and prognostic biomarkers for prostate cancer has great potential.
Review
Chemistry, Analytical
Tomas Bertok, Aniko Bertokova, Stefania Hroncekova, Erika Chocholova, Natalia Svecova, Lenka Lorencova, Peter Kasak, Jan Tkac
Summary: The review gives an overview of prostate cancer (PCa) incidence, mortality, and diagnostic methods, highlighting the need for novel biomarkers. In addition to prostate specific antigen, the review discusses alternative biomarkers available for PCa diagnostics, including low and high molecular mass biomolecules.
Article
Chemistry, Analytical
Balazs Reider, Gabor Jarvas, Jana Krenkova, Andras Guttman
Summary: Prostate cancer, with the highest malignancy rate in men worldwide, has seen a steep increase in false positive diagnoses despite the use of the gold standard PSA assays to reduce mortality. Complementary biomarkers are urgently needed to enhance the specificity and selectivity of current diagnostic methods.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Review
Andrology
Tushar Aditya Narain, Prasanna Sooriakumaran
Summary: PSA is a well-known biomarker used for screening, diagnosis, and follow-up of prostate cancer patients. Due to its limitations, newer blood and urinary biomarkers have been evaluated to improve detection and risk stratification. A combination of these markers, clinical risk factors, and advances in imaging offer the potential for better diagnostic performance and clearer risk stratification to guide therapeutics.
WORLD JOURNAL OF MENS HEALTH
(2022)
Article
Chemistry, Analytical
Balazs Reider, Eszter Gacsi, Hajnalka Jankovics, Ferenc Vonderviszt, Tibor Szarvas, Andras Guttman, Gabor Jarvas
Summary: This paper describes the development and implementation of a high-throughput capillary electrophoresis-based glycan analysis workflow for urinary PSA, which shows excellent selectivity and high resolution, making it a valuable tool for distinguishing different forms of prostate cancer.
ANALYTICA CHIMICA ACTA
(2021)
Article
Oncology
William Butler, Colin McDowell, Qing Yang, Yiping He, Yue Zhao, J. Spencer Hauck, Yinglu Zhou, Hong Zhang, Andrew J. Armstrong, Daniel J. George, Richard Drake, Jiaoti Huang
Summary: The glycome in prostate cancer undergoes significant changes during disease progression and therapy resistance, with specific glycosylation patterns correlating with remission, resistance, recurrence, and neuroendocrine differentiation. These findings have important implications for the diagnosis and treatment of prostate cancer.
NPJ PRECISION ONCOLOGY
(2023)
Article
Oncology
Bei Xu, Yan Chen, Xi Chen, Lingling Gan, Yamei Zhang, Jiafu Feng, Lin Yu
Summary: The study suggests that metabolic reprogramming, particularly in lipid metabolism, is a key feature of PCa. The 18 lipid or lipid-associated metabolites identified may serve as potential diagnostic markers for distinguishing between PCa patients and BPH individuals with PSA levels in the 4-10 ng/ml gray zone.
FRONTIERS IN ONCOLOGY
(2021)
Article
Urology & Nephrology
Marlon Perera, Lewis Smith, Ian Thompson, Geoff Breemer, Nathan Papa, Manish I. Patel, Peter Swindle, Elliot Smith
Summary: Machine learning models can enhance the diagnostic utility of PSA kinetics in the diagnosis of prostate cancer, demonstrating significant improvements in accuracy compared to traditional approaches.
EUROPEAN UROLOGY FOCUS
(2022)
Article
Cardiac & Cardiovascular Systems
Koki Mise, Mariko Imamura, Satoshi Yamaguchi, Mayu Watanabe, Chigusa Higuchi, Akihiro Katayama, Satoshi Miyamoto, Haruhito A. Uchida, Atsuko Nakatsuka, Jun Eguchi, Kazuyuki Hida, Tatsuaki Nakato, Atsuhito Tone, Sanae Teshigawara, Takashi Matsuoka, Shinji Kamei, Kazutoshi Murakami, Ikki Shimizu, Katsuhiro Miyashita, Shinichiro Ando, Tomokazu Nunoue, Michihiro Yoshida, Masao Yamada, Kenichi Shikata, Jun Wada
Summary: The study found that urinary glycan signals binding to two specific lectins were significantly associated with CVE, particularly the high-mannose type of N-glycans. Adding the glycan index for UDA to the model improved outcome prediction significantly.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Oncology
Danielle S. Bitterman, Ming-Hui Chen, Jing Wu, Andrew A. Renshaw, Marian Loffredo, Philip W. Kantoff, Eric J. Small, Anthony V. D'Amico
Summary: This study found that low testosterone level at PSA failure in high-risk patients with PC treated with RT is associated with increased PCSM and ACM risk, and that higher PSAn is also associated with worse PCSM and ACM risk, especially in men with normal testosterone levels at PSA failure.
Article
Biophysics
Corinne Dejous, Uma Maheswari Krishnan
Summary: Prostate cancer is a common form of cancer in men, with high mortality rates due to late diagnosis. Conventional diagnostic methods, such as the PSA biomarker, have limitations, leading to a search for novel biomarkers. Advancement in sensor technologies using nanomaterials may offer a rapid and sensitive alternative for detection of non-PSA markers.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Benildo Sousa Cavada, Messias Vital Oliveira, Vinicius Jose Silva Osterne, Vanir Reis Pinto-Junior, Francisco William Viana Martins, Cornevile Correia-Neto, Ronald Feitosa Pinheiro, Rodrigo Bainy Leal, Kyria Santiago Nascimento
Summary: Poor lifestyle choices and genetic predisposition contribute to the increase in cancer cases, such as breast cancer. The development of new strategies for early detection and treatment options is needed to improve survival rates. Glycans on cancer cell surfaces serve as efficient tumor cell markers and can be recognized by lectins. Plant lectins have potential applications in diagnosing and treating breast cancer by detecting and sorting glycans in healthy and malignant cells. Furthermore, lectins can be utilized in various ways, such as mapping differentially expressed glycans, histochemistry/cytochemistry analysis, lectin arrays, chromatographic matrices, drug delivery, and biosensing.
Article
Biochemistry & Molecular Biology
Aniko Bertokova, Tomas Bertok, Eduard Jane, Michal Hires, Petra Dubjakova, Olga Novotna, Vitazoslav Belan, Juraj Fillo, Jan Tkac
Summary: This study found that LacdiNAc on fPSA can serve as a biomarker for monitoring PCa development and progression. Genetic algorithm was applied to identify suspicious individuals in a BPH cohort. Furthermore, it is suggested for the first time that changed glycosylation on PSA can be used to identify CRPCa patients.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Tung-Shing Mamie Lih, Mingming Dong, Leslie Mangold, Alan Partin, Hui Zhang
Summary: This study investigated the clinical utility of urinary marker panels composed of urinary glycopeptides and/or urinary prostate-specific antigen (PSA) for distinguishing non-aggressive from aggressive prostate cancer. The multi-marker panels showed clinically meaningful results with area under the curve (AUC) ranging from 0.70 to 0.85.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, General & Internal
Alicja Popiolek, Bartosz Brzoszczyk, Piotr Jarzemski, Aleksandra Chyrek-Tomaszewska, Radoslaw Wieczor, Alina Borkowska, Maciej Bielinski
Summary: PSA levels at a distant postoperative time and free T level after hormonal treatment may be biomarkers of cognitive function. There was a strong correlation between PSA levels and verbal memory and executive function tests, with a trend observed for visuospatial memory. Free T levels after hormonal treatment were significantly correlated with executive functions, with the impact of comorbid diabetes on these correlations.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Chemistry, Analytical
Anna Blsakova, Filip Kveton, Lenka Lorencova, Ola Blixt, Alica Vikartovska, Peter Kasak, Jan Tkac
Summary: This study presents the development of a novel SUspension Magnetic-Bead-based Assay (SUMBA) for the detection of antibodies against aberrant glycans (AGA) as potential cancer biomarkers. The SUMBA method was extensively optimised to achieve ultrasensitive detection of AGA with high recovery rates in serum samples.
ANALYTICA CHIMICA ACTA
(2022)
Article
Polymer Science
Ana Antunes, Anton Popelka, Adriaan Stephanus Luyt, Abdelrahman Mahmoud, Omar Yosef Aljarod, Mohamed Hassan, Peter Kasak
Summary: This study demonstrated the potential use of corona and RF plasma treatment on improving the degradability of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) through hydrolytic and UV degradation promotion.
EXPRESS POLYMER LETTERS
(2022)
Article
Chemistry, Physical
Muhammad Imran, Ammar Bin Yousaf, Muhammad Farooq, Peter Kasak
Summary: The combination of BiVO4 nanorods and ZnCdS in a hetero-structure has shown great potential in improving the photocatalytic activity for hydrogen production through water splitting under visible light irradiation. The heterostructure exhibits enhanced visible light harvesting ability, prolongs the lifespan of charge carriers, and improves charge separation efficiency, leading to increased efficiency in water splitting.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Multidisciplinary Sciences
David Gallo, Jordan T. F. Young, Jimmy Fourtounis, Giovanni Martino, Alejandro Alvarez-Quilon, Cynthia Bernier, Nicole M. Duffy, Robert Papp, Anne Roulston, Rino Stocco, Janek Szychowski, Artur Veloso, Hunain Alam, Prasamit S. Baruah, Alexanne Bonneau Fortin, Julian Bowlan, Natasha Chaudhary, Jessica Desjardins, Evelyne Dietrich, Sara Fournier, Chloe Fugere-Desjardins, Theo Goullet de Rugy, Marie-Eve Leclaire, Bingcan Liu, Vivek Bhaskaran, Yael Mamane, Henrique Melo, Olivier Nicolas, Akul Singhania, Rachel K. Szilard, Jan Tkac, Shou Yun Yin, Stephen J. Morris, Michael Zinda, C. Gary Marshall, Daniel Durocher
Summary: Amplification of the CCNE1 locus on chromosome 19q12 is common in various tumor types, especially in high-grade serous ovarian cancer, uterine tumors, and gastro-esophageal cancers. The amplification of CCNE1 is associated with genome instability, high levels of cyclin E, and resistance to cytotoxic and targeted therapies. Through genome-scale CRISPR-Cas9-based synthetic lethality screens, it was discovered that increasing CCNE1 dosage creates vulnerability to the inhibition of the PKMYT1 kinase, a negative regulator of CDK1. Inhibiting PKMYT1 through the development of RP-6306, an orally available and selective inhibitor, shows single-agent activity and durable tumor regressions when combined with gemcitabine in models of CCNE1 amplification. This treatment activates CDK1 selectively in CCNE1-overexpressing cells, promoting early mitosis in cells undergoing DNA synthesis, which is disrupted by CCNE1 overexpression. Thus, PKMYT1 inhibition is a promising therapeutic strategy for CCNE1-amplified cancers.
Article
Medicine, Research & Experimental
Aniko Bertokova, Natalia Svecova, Katarina Kozics, Alena Gabelova, Alica Vikartovska, Eduard Jane, Michal Hires, Tomas Bertok, Jan Tkac
Summary: This article examines the best procedure for isolating exosomes, which are considered to be a rich source of biomarkers. The study compared several isolation procedures and found that magnetic-based kits were the most effective. It also determined that storing isolated exosomes at -20°C is optimal and observed that cancerous prostate cell lines produce more exosomes than non-cancerous cell lines. Additionally, the study explored the response of cell lines to stress and investigated the effect of Triton X-100 on exosome lysis. The article concludes by discussing the advantages of using a self-made 2D biochip for monitoring exosome interactions and the potential of lectin fluorescent microarrays for analyzing glycans in lysed exosomes.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Chemistry, Analytical
Ammar Bin Yousaf, Filip Kveton, Anna Blsakova, Anton Popelka, Jan Tkac, Peter Kasak
Summary: The study introduces a novel method to modify the surface chemistry of commercial tungsten carbide electrocatalyst using electrochemical oxygen reduction reactions. The modified tungsten carbide exhibits enhanced electrocatalytic activities in fuel cells, making it a promising material for applications in fuel cell technology.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Veronika Vrablova, Anna Blsakova, Lenka Lorencova, Jozef Kollar, Alica Vikartovska, Peter Kasak, Jan Tkac
Summary: An assay for detection of the cancer biomarker Thomsen-nouvelle (Tn) antigen on ELISA plates was developed and optimized in this study. The effect of size and charge density of magnetic beads on the sensitivity of the assay was investigated. The results provided valuable insights into the immobilization process on the nanoscale and achieved highly sensitive and selective detection of the analyte.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biochemistry & Molecular Biology
Bence Farkas, Hana Vojtkova, Zuzana Farkas, Domenico Pangallo, Peter Kasak, Antonio Lupini, Hyunjung Kim, Martin Urik, Peter Matus
Summary: Manganese oxides are crucial in natural geochemical barriers, as they have reactive properties towards essential and potentially toxic trace elements. Microorganisms can actively alter conditions in their microenvironment and dissolve minerals, as well as precipitate bioavailable manganese ions into biogenic minerals. These microbial transformations significantly impact the geochemistry of manganese and elements associated with its oxides, potentially leading to environmental consequences.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Marketa Ilcikova, Miroslav Mrlik, Martin Cvek, Dmitrij Bondarev, Zuzana Kronekova, Juraj Kronek, Peter Kasak, Jaroslav Mosnacek
Summary: Atom transfer radical polymerization (ATRP) and surface-initiated ATRP (SI-ATRP) were optimized to synthesize polymers with well-defined molar mass and narrow dispersity. The polymerization procedure was thoroughly investigated and optimized, leading to successful preparation of poly-(2-isopropenyl-2-oxazoline)(PIPOx) with controlled molar masses and dispersity. Additionally, magnetic CI-PIPOx core-shell particles with different grafted PIPOx molar masses were synthesized for potential applications as embolization agents.
Article
Materials Science, Multidisciplinary
Anton Popelka, Anjali Cheeramthodi Padmanabhan, Ahmed Sedeek Elgendy, Patrik Sobolciak, Igor Krupa, Ammar Bin Yousaf, Martin Sebesta, Jan Tkac, Peter Kasak
Summary: In this study, a stable and highly hydrophobic perfluoroctylsilane grafted Ti3C2X (PFMXene) was used to fabricate liquid marble and hydrogel-based liquid marble. The hydrogel-based liquid marbles demonstrated floating ability and self-assembly on the water's surface. Additionally, the controlled non-contact movement of hydrogel marbles in water reached a speed of up to 2 cm/s, and the hydrogel matrix allowed for controlled light-induced disintegration or application as a water evaporation system.
MATERIALS TODAY COMMUNICATIONS
(2023)
Article
Engineering, Biomedical
Stefania Hroncekova, Lenka Lorencova, Tomas Bertok, Michal Hires, Eduard Jane, Marek Bucko, Peter Kasak, Jan Tkac
Summary: Screen-printing technology is used to immobilize enzyme sarcosine oxidase (SOx) onto screen-printed carbon electrodes, creating a portable nanobiosensor for ultrasensitive detection of prostate cancer biomarker sarcosine. The nanobiosensor shows a high detection sensitivity of 7.0 nM using minimal sample volume and exhibits linear calibration curves in the concentration range of 5 nM to 50 μM. The device has a high recovery index and can be used for detection of sarcosine in urine for at least a period of 5 weeks.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
