Journal
BIOMASS & BIOENERGY
Volume 78, Issue -, Pages 156-163Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biombioe.2015.04.019
Keywords
Ethanol; Kluyveromyces marxianus; Rice straw; Nutrient supplementation; Simultaneous saccharification and fermentation
Funding
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil)
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil)
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil)
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The effect of nutrient supplementation on ethanol production by recently selected thermotolerant yeast (Kluyveromyces marxianus NRRL Y-6860) was investigated in different strategies of saccharification and fermentation employing rice straw pretreated by dilute acid. Among the evaluated strategies, similar ethanol yields (Y-p/s similar to 0.23 g g(-1)) were obtained with or without nutrient addition. However, considering the whole process time, the strategy based on simultaneous saccharification and fermentation (SSF), without prehydrolysis, was assigned as the most suitable configuration due to the highest ethanol volumetric productivity (1.4 g L-1 h(-1)), about 2-fold higher in relation to the others. The impact of enzymatic preparation employed in this study was also evaluated on glucose fermentation in semi-synthetic medium. The enzymatic preparation affected both glucose consumption and ethanol production by K. marxianus NRRL Y-6860, but just in the absence of nutrients. Therefore, the enzyme type and loading should be carefully defined, not only by the capital costs involved, but also by the possibility of increasing the fermentation inhibitors. (C) 2015 Published by Elsevier Ltd.
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