Review
Biochemistry & Molecular Biology
Haim Breitbart, Elina Grinshtein
Summary: Capacitation is a process in which mammalian sperm undergo a series of biochemical reactions in the female reproductive tract, protecting the sperm from spontaneous acrosome reaction and ensuring successful fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Yael Azoulay, Zvi Malik, Haim Breitbart
Summary: Bacterial contamination in semen can deteriorate sperm function, potentially by inducing a premature form of the acrosome reaction. To understand the mechanism, the effects of bacteria on sperm acrosome reaction and other parameters involved in sperm capacitation were studied.
Article
Pharmacology & Pharmacy
Shehreen Amjad, Md Saidur Rahman, Won-Ki Pang, Do-Yeal Ryu, Elikanah Olusayo Adegoke, Yoo-Jin Park, Myung-Geol Pang
Summary: In this study, exposure to a phthalate mixture resulted in decreased sperm motility and hyperactivity, compromised sperm ability to generate ATP, induced abnormal capacitation and acrosome reaction, upregulated protein tyrosine phosphorylation via a protein kinase-A-dependent pathway, and significantly affected fertilization and early embryonic development. The findings suggest that the phthalate mixtures adversely affected sperm processes and ultimately led to poor fertilization rates and repressed embryonic development, with the lowest observed adverse effect dose being <1 µg/mL in vitro.
Article
Agriculture, Dairy & Animal Science
Vitaly Denisenko, Irena Chistyakova, Natalia Volkova, Ludmila Volkova, Baylar Iolchiev, Tatyana Kuzmina
Summary: Progesterone affects the functional status of bovine spermatozoa by inducing capacitation and acrosome reactions. Prolactin and dibutyryl cyclic adenosine monophosphate can modulate these effects in the presence of progesterone.
Review
Biochemistry & Molecular Biology
Filip Benko, Dana Urminska, Michal Duracka, Eva Tvrda
Summary: This review provides an overview of specific ion channels and transporters located in the mammalian sperm plasma membrane and their functional roles in sperm development and function. It discusses the potential therapeutic targets for infertility management.
Article
Agriculture, Dairy & Animal Science
Laura Robles-Gomez, Leopoldo Gonzalez-Brusi, Paula Saez-Espinosa, Natalia Huerta-Retamal, Paula Cots-Rodriguez, Manuel Aviles, Maria Jose Gomez-Torres
Summary: The study revealed that different in vitro capacitation times and acrosome reactions can affect the specific location of boar sperm glycoconjugates, showing distinct binding patterns for different lectins at different stages.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Developmental Biology
Elina Grinshtain, Sally Shpungin, Micha Baum, Uri Nir, Haim Breitbart
Summary: The physiological acrosome reaction occurs after the process of capacitation in mammalian spermatozoa. The study reveals a novel pathway involving the tyrosine kinase Fer that prevents spontaneous acrosome reaction by enhancing actin polymerization.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Andrology
Luisa Ramirez-Agamez, Camilo Hernandez-Aviles, Isabel Ortiz, Charles C. Love, Dickson D. Varner, Katrin Hinrichs
Summary: This study demonstrates that incubation with a specific energy substrate, lactate, is associated with a spontaneous acrosome reaction in stallion spermatozoa. The proportion of live, acrosome-reacted spermatozoa obtained is among the highest reported for equine sperm. These findings contribute to our understanding of stallion sperm physiology.
Article
Agriculture, Dairy & Animal Science
Phillip H. Purdy, James K. Graham, Hymerson C. Azevedo
Summary: This study used flow cytometry to evaluate the viability and capacitation of sperm and investigated the effects of different freezing and capacitating media. The results showed that different media induced different responses in sperm capacitation and function.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Environmental Sciences
Lijun Shan, Yuhao Chai, Tian Gao, Kun Li, Jianmin Yu, Fei Liang, Ya Ni, Peibei Sun
Summary: PFOS and PFOA, persistent organic pollutants, have harmful effects on human health, especially on male reproduction. They inhibit sperm capacitation and fertilization by affecting hormone levels, spermatogenesis, and sperm quality. The exact mechanisms are still unclear.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
David Martin-Hidalgo, Beatriz Macias-Garcia, Lauro Gonzalez-Fernandez
Summary: Increasing sperm concentration stimulates capacitation and acrosome reaction in boars, accompanied by plasma membrane lipid disorganization and increased sperm viability.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Obstetrics & Gynecology
David Martin-Hidalgo, Lauro Gonzalez-Fernandez, M. Julia Bragado, Luis J. Garcia-Marin, Marco G. Alves, Pedro F. Oliveira
Summary: YK 3-237 induces capacitation-related events in human spermatozoa, such as an increase in tyrosine phosphorylation levels and acrosome-reacted spermatozoa after the ionophore challenge. These effects are independent of protein lysine acetylation but depend on bicarbonate and calcium levels.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Andrology
Nirmal Chhikara, Anil Kumar Tomar, Sudip Kumar Datta, Savita Yadav
Summary: This study investigates the changes in sperm proteins during in vitro capacitation and acrosome reaction, aiming to uncover unknown molecular aspects of fertilization and their impact on male fertility. A total of 1088 sperm proteins were identified, with significant differences in expression between capacitated and acrosome reacted spermatozoa. The proteomic changes associated with asthenozoospermia were also observed. The findings contribute to a better understanding of the processes involved in fertilization and male infertility.
Article
Anatomy & Morphology
Paula Saez-Espinosa, Alba Lopez-Huedo, Laura Robles-Gomez, Natalia Huerta-Retamal, Jon Aizpurua, Maria Jose Gomez-Torres
Summary: Research has shown a strong connection between head ConA-binding sites and flagellar tyrosine phosphorylation during spermatozoa capacitation, providing insights into the sperm subpopulations based on critical physiological events like capacitation and acrosome reaction. These findings could potentially have implications in improving sperm selection techniques.
CELLS TISSUES ORGANS
(2021)
Article
Biochemistry & Molecular Biology
Paula A. Balestrini, Claudia Sanchez-Cardenas, Guillermina M. Luque, Carolina Baro Graf, Jessica M. Sierra, Arturo Hernandez-Cruz, Pablo E. Visconti, Dario Krapf, Alberto Darszon, Mariano G. Buffone
Summary: The process of sperm capacitation is essential for fertilization capacity, resulting in acrosomal exocytosis. Em hyperpolarization has been shown to be necessary and sufficient to prepare human sperm for acrosomal exocytosis. This change in Em decreases Ca2+ oscillations, providing experimental evidence for the molecular mechanism underlying acrosomal responsiveness.
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.