Journal
STEM CELLS
Volume 37, Issue 9, Pages 1151-1157Publisher
WILEY
DOI: 10.1002/stem.3041
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Funding
- NCI NIH HHS [P30 CA124435] Funding Source: Medline
- NIGMS NIH HHS [DP2 GM123485] Funding Source: Medline
- NIH HHS [S10 OD018220] Funding Source: Medline
- NLM NIH HHS [T15 LM007033] Funding Source: Medline
- U.S. National Institutes of Health [S10OD018220] Funding Source: Medline
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Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator system adapted into hPSCs and perform RNA sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs toward differentiation. Stem Cells 2019;37:1151-1157
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