Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 296, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.05.095
Keywords
Pencil graphite electrode; Microelectrode array; HPLC; Electrochemical detection; Electrochemical flow cell
Funding
- Grant Agency of the Czech Republic [15-05198S]
- Palacky University [IGA_PrF_2018_027]
Ask authors/readers for more resources
A concentric thin layer cell accommodating a non-standard 0.2 mm diameter pencil graphite disposable working electrode is described. The cell was installed into HPLC manifold as an electrochemical detector. Trouble-free operation in mobile phases containing both low and high content of organic solvents is demonstrated by HPLC analyses of phenolic acids and tocopherol isomers. The data obtained from HPLC separation of model mixtures of gentisic, caffeic and dihydrocaffeic acids show a remarkable electrolytic efficiency exceeding 80% at 200, and 50% at 500 mu L min(-1), while for gentisic acid the limit of detection (LOD) was 0.4 nmol L-1 at 20 microliter sample loading (8 fmol on-column). Similar performances were found in non-aqueous mobile phase, where a LOD of 0.8 nmol L-1 was achieved for delta-tocopherol. The developed flow-through detector is designed to allow easy replacement of pencil graphite working electrode in a highly reproducible manner. The relative standard deviation for the HPLC analysis of tocopherol isomers was of 5.3% (n=3, C=500 nmol L-1). The combination of simple construction, excellent electrochemical performance and hydrodynamics identical to that of commercial UV-VIS HPLC detector suggests that the proposed device is a viable low-cost alternative to commercially available electrochemical detectors.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available