Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 285, Issue -, Pages 62-67Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.01.025
Keywords
Sensor; Matrix effect; Mercury; Ionic liquid; DNAzyme
Funding
- National Natural Science Foundation of China [21675112]
- Key project of science and technology plan of Beijing Education Commission [KZ201710028027]
- Hundred, Thousand, and Ten thousand Talent Projects in Beijing [2018A38]
- Beijing Natural Science Foundation [L182046]
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Heavy metal sensors are limited to the applications for environmental and drinking waters due to matrix interference. We report here a task-specific ionic liquid enabled DNAzyme-based mercury sensor (TSIL-Hg-sensor), allowing the sensitive detection of mercury in solid food samples. In TSIL-Hg-sensor, TSILs sequentially extracted mercury ion from microwave acid digestion solution with high efficiency, and then peroxidase-mimicking DNAzyme (PMD) that was used for the following signal amplification. Hg2+ and PMD shared the extraction capacity of TSILs. The greater amount of Hg2+ extracted from the first extraction would results in the less PMD from the second extraction, enabling the signal-on colorimetric detection of mercury. The detection of limit was 0.5 nM when [OMIM][PF6] was used for the extractions, 100 times more sensitive than the previously reported PMD-based signal-off sensor. The sensor with [Opy][BF4] was less sensitive, but highly specific for Hg2+. The practical applications were demonstrated by challenging TSIL-Hg-sensor with 5 standard solid foods containing mercury from 5.3 to 850 mu g/kg with the recover percentage of 102 (similar to)115%. TSILs are designable for almost any molecules besides metal ions. Nucleic acids are compatible with numerous signal amplification means. TSIL-Hg-sensor therefore represents a universal design for sensors with high matrix interference resistance.
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