Journal
BIOMARKERS
Volume 20, Issue 3, Pages 212-217Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/1354750X.2015.1062918
Keywords
Bilharziasis; bladder cancer; gold nanoparticles; long non-coding RNA-UCA1; urine biomarkers; urine cytology
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Funding
- Ain Shams University
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We developed a specific hybridization assay for direct detection of long non-coding RNA urothelial carcinoma associated-1 (lncRNA-UCA1). Total RNA was extracted from urine pellet samples (bladder carcinoma patients and controls). Then, we compared the developed nanoassay with quantitative real time polymerase chain reaction (qRT-PCR) results in detection of urine UCA1 in bladder cancer and control samples. The sensitivity and the specificity of UCA1 nanoassay were 92.1% and 93.3%, respectively. The concordance of the two methods was 98%. Interestingly, all bilharzial benign cases showed negative lncRNA-UCA1 using both methods. UCA1-nanoassay is a valid test for direct detection of urine UCA1 for bladder cancer detection.
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