Article
Biochemistry & Molecular Biology
Taisuke Watanabe, Yutaka Kitamura, Hachidai Aizawa, Hideo Masuki, Tetsuhiro Tsujino, Atsushi Sato, Hideo Kawabata, Kazushige Isobe, Koh Nakata, Tomoyuki Kawase
Summary: This study optimized quantification conditions and applied DAPI probe to assess platelet polyP levels in a Japanese population. Results showed significantly higher polyP levels in women compared to men, with a moderate negative correlation between age and polyP levels in women. Additionally, women exhibited significantly higher responsiveness to CaCl2 stimulation than men. This DAPI-based protocol may serve as an efficient and cost-effective method for routine laboratory testing of platelet polyP levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Zhaoyan Tian, Yafeng Wu, Fengying Shao, Dezhi Tang, Xiang Qin, Chenchen Wang, Songqin Liu
Summary: An integrated microfluidic system was designed for real-time monitoring of glycan expression on the surface of single tumor cells, achieving a level of 5.0 x 10^8 molecules per cell. This platform has the capability to distinguish single cancer cells from normal cells based on the expression level of glycans, providing a simple and accessible method for single-cell analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Plant Sciences
Tim Rammler, Frank Wackenhut, Johanna Rapp, Sven Zur Oven-Krockhaus, Karl Forchhammer, Alfred J. J. Meixner, Klaus Harter
Summary: Photosynthesis is a vital biological process that produces oxygen and is the foundation for various plant products. Measurement of photosynthetic properties can provide insights into the physiological conditions of organisms. A new method using an optical Fabry-Perot microcavity allows for the assessment of photosynthetic activity and properties for individual cyanobacteria.
Article
Microbiology
Antonio Llop, Jose I. Labella, Marina Borisova, Karl Forchhammer, Khaled A. Selim, Asuncion Contreras
Summary: The cyanobacterial protein PipY belongs to the PLP-binding proteins family and is involved in the homeostasis of vitamin B-6 vitamers and amino/keto acids. It is connected with PipX in the cyanobacteria genomic context and involved in signaling the intracellular energy status and carbon-to-nitrogen balance. While the function of PipY is still uncertain, preliminary data suggests its involvement in signaling pathways related to the stringent stress response.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Computer Science, Artificial Intelligence
Gyuhyun Lee, Jeong-Woo Oh, Nam-Gu Her, Won-Ki Jeong
Summary: The proposed method in this paper aims to transform bright-field microscopy images into synthetic fluorescence images for observing cell apoptosis, nuclei, and cytoplasm, commonly used in drug screening. By leveraging deep learning and multi-task learning with adversarial losses, the proposed method generates accurate and realistic synthetic fluorescence images, reducing preparation time and improving screening efficiency.
MEDICAL IMAGE ANALYSIS
(2021)
Article
Biotechnology & Applied Microbiology
Yavuz S. Yalcin, Busra Aydin, Huan Chen, Samson Gichuki, Viji Sitther
Summary: In this study, the effects of nanoscale zero-valent iron nanoparticles (nZVIs) and ampicillin on lipid production and cellular structural changes in Fremyella diplosiphon strain B481-SD were investigated. The results showed that the individual application of 0.8 mg/L ampicillin, 3.2 mg/L nZVIs, and a combined regimen of both significantly increased total lipid abundance, fatty acid methyl ester (FAME) compositions, and alkene production. In addition, the combination regimen had a significant effect on monounsaturated fatty acids (MUFAs) and membrane lipids. Transmission electron microscopy studies revealed the presence of complex stacked membranes in the treated F. diplosiphon.
MICROBIAL CELL FACTORIES
(2023)
Article
Chemistry, Multidisciplinary
Guankui Wang, Markella Zannikou, Laren Lofchy, Yue Li, Hanmant Gaikwad, Irina Balyasnikova, Dmitri Simberg
Summary: The type of fluorescent label on liposomes significantly impacts their extravasation and accumulation in tumors. In animal experiments, indocarbocyanine lipids (ICLs) were found to spread and accumulate in tumor tissue more easily compared to fluorescent phospholipids (FPLs).
Article
Biochemical Research Methods
Miriam C. Bassler, Tim Rammler, Frank Wackenhut, Sven Zur Oven-Krockhaus, Ivona Secic, Rainer Ritz, Alfred J. Meixner, Marc Brecht
Summary: This study investigates the distribution and penetration behavior of hypericin in glioma cell spheroids using fluorescence microscopy and FLIM. The results show that hypericin exhibits a gradient distribution towards the spheroid core for short incubation periods or small concentrations, while a homogeneous distribution is observed for long incubation times or high concentrations.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Liya Ma, Juan Francisco Guerra Maldonado, Arash Zamyadi, Sarah Dorner, Michele Prevost
Summary: There has been an increase in potentially toxic cyanobacterial blooms in drinking water sources. This study validates in situ probes for detecting cyanobacterial breakthrough in drinking water treatment plants. The results show that the probes successfully detect high cyanobacterial biovolumes in the clarification process.
ENVIRONMENTAL MONITORING AND ASSESSMENT
(2023)
Article
Biochemical Research Methods
Ellen D. Zhong, Tristan Bepler, Bonnie Berger, Joseph H. Davis
Summary: cryoDRGN is an algorithm that uses the representation power of deep neural networks to directly reconstruct continuous distributions of 3D density maps and map per-particle heterogeneity of single-particle cryo-EM datasets. It can uncover residual heterogeneity in high-resolution datasets and visualize large-scale continuous motions of protein complexes, while also offering interactive tools for dataset visualization and analysis.
Article
Chemistry, Physical
Y. Yang, J. Cui, H. J. Guo, X. Shen, Y. Yao, R. C. Yu, R. Wen
Summary: This study investigates the Li-ion transport mechanism in solid-state electrolytes using real-time in situ techniques, revealing the presence of inhomogeneous Li-ion accumulation during charging and suggesting that grain boundaries may have a negative impact on ion conductivity. These findings contribute to a better understanding of interfacial mechanisms and the optimization of solid-state batteries.
JOURNAL OF MATERIALS CHEMISTRY A
(2021)
Review
Biochemistry & Molecular Biology
Ivan Kushkevych, Blanka Hyzova, Monika Vitezova, Simon K. -M. R. Rittmann
Summary: This paper explores microscopic methods for identifying sulfate-reducing bacteria (SRB), discussing their presence in various habitats and the techniques used for their detection. It also highlights the economic and health implications of SRB in industries and human microbiota, emphasizing the importance of methods for detecting these microorganisms, including molecular and fluorescence techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Jeonggeun Song, Juehyung Kang, Ungyo Kang, Hyeong Soo Nam, Hyun Jung Kim, Ryeong Hyeon Kim, Jin Won Kim, Hongki Yoo
Summary: Two-photon microscopy (TPM) is a biomedical imaging method with a strong ability for deep penetration and optical sectioning. In this study, we used a pulse picker to increase the pulse peak power of the excitation source, leading to higher emission of two-photon fluorescence. By applying a time gating detection method, we were able to significantly reduce the background noise of the images.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Soorya Pradeep, Tasmia Tasnim, Huanan Zhang, Thomas A. Zangle
Summary: Measurement of neuron behavior is crucial for studying neural development and evaluating potential therapies. This paper presents a label-free, quantitative method using QPI to separately measure the mass accumulation rates of soma and neurites, as well as to quantify the processes of maturation and formation of neurites. The approach simplifies conventional neurite outgrowth measurements while providing key data on resources used for neurite production during neural development.
Article
Chemistry, Applied
Alexey A. Kostyukov, Mikhail G. Mestergazi, Anton E. Egorov, Anna M. Shmykova, Anna Shibaeva, Alina A. Markova, Tatyana A. Podrugina, Vladimir I. Pogonin, Eugene Radchenko, Vladimir A. Palyulin, Alexander A. Shtil, Tatiana D. Nekipelova, Iouri E. Borissevitch, Vladimir A. Kuzmin
Summary: This study investigated the photophysical characteristics of a new bis-carbocyanine dye (BCD) and its interaction with human serum albumin (HSA) and DNA. The binding constants of BCD to HSA and DNA were determined, and the fluorescence quantum yield of BCD increased 10-fold after binding to HSA.
Article
Biotechnology & Applied Microbiology
Elena Kupriyanova, Maria A. Sinetova, Anna Leusenko, Alexander S. Voronkov, Dmitry A. Los
Summary: This study expressed different forms of EcaA protein in Escherichia coli and found that the recombinant proteins with a leader peptide could form mature EcaA in the outer layers of the cells, showing high extracellular carbonic anhydrase activity. The cyanobacterial signal peptide played an important role in the secretion machinery of E. coli.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Maria Breygina, Alexander Voronkov, Ilshat Galin, Guzel Akhiyarova, Svetlana Polevova, Ekaterina Klimenko, Igor Ivanov, Guzel Kudoyarova
Summary: This study used ELISA and immunolocalization techniques to investigate the changes in endogenous hormone levels and their localization in spruce pollen. The results showed significant fluctuations in hormone levels during pollen activation and polar growth, with variations in hormone localization between different cells.
Article
Chemistry, Physical
Ayshat M. Bozieva, Makhmadyusuf Kh. Khasimov, Roman A. Voloshin, Maria A. Sinetova, Elena V. Kupriyanova, Sergey K. Zharmukhamedov, Dmitry O. Dunikov, Anatoly A. Tsygankov, Tatsuya Tomo, Suleyman I. Allakhverdiev
Summary: This study aims to investigate the H2-releasing ability of new strains of cyanobacteria and evaluate the effects of the photosystem II inhibitor DCMU on H2 production under light and dark conditions. The results demonstrate that cultures treated with DCMU produced several times more H2 than untreated cells. The highest H2 photoproduction rate of 4.24 mmol H2 (mg Chl a h)-1 was observed in Dolichospermum sp. IPPAS B.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Plant Sciences
Ilya V. Seregin, Tatiana V. Ivanova, Alexander S. Voronkov, Anna D. Kozhevnikova, Henk Schat
Summary: This pilot study aimed to compare the effects of zinc and nickel on the fatty acid profiles, oxidative stress and desaturase activity in two different species of Arabidopsis plants, one being a zinc hyperaccumulator and the other being a metal excluder. The results showed that significant changes in fatty acid composition were observed in both species, but the changes were metal-dependent and species-specific. These metal-induced changes in fatty acid composition could affect the fluidity and stability of membranes in the plants, potentially contributing to their metal tolerance or acclimation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Plant Sciences
T. V. Ivanova, A. S. Voronkov
Summary: The vegetative organs of common basil grown in water culture at different levels of illumination were studied. The intensity of illumination showed significant effects on the fatty acid composition and morphophysiological parameters of the plants. Increasing the intensity of lighting to certain values directly affected the physiological parameters of the basil plants.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
D. A. Gabrielyan, M. A. Sinetova, A. K. Gabrielyan, L. A. Bobrovnikova, V. S. Bedbenov, A. Yu. Starikov, A. A. Zorina, B. V. Gabel, D. A. Los
Summary: This paper presents a laboratory system for intensive cultivation of microalgae and cyanobacteria, and showcases the results of experiments conducted using this system.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
L. A. Khalilova, A. S. Voronkov
Summary: Plants undergo various stress factors throughout their lifespan, and their plasma membrane is responsible for adapting to these stresses through protein remodeling via endocytosis. This study focused on the role of Flot1 protein in the endocytosis process. By inhibiting clathrin-mediated endocytosis and depleting the plasma membrane of sterols, morphological changes in the root cells were observed. In the mutant plants, the Golgi complex structure and early endosomes formation were affected under these conditions, suggesting that the microdomain-associated Flot1 protein plays a crucial role in maintaining Golgi complex structure and early endosome formation.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Elena Krivina, Maria Sinetova, Tatyana Savchenko, Evgeniy Degtyaryov, Elizaveta Tebina, Anna Temraleeva
Summary: The study on Micractinium genus species diversity is still relevant due to its simple morphology and high phenotypic plasticity. These studies have practical significance as some of these species have high biotechnological potential, capable of accumulating valuable metabolites, including polyunsaturated fatty acids. The studied strains were identified as two new species, M. lacustre and M. thermotolerans, based on morphological characteristics, habitat, phylogenetic analysis, and fatty acids profile.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2023)
Article
Biodiversity Conservation
Elena S. Krivina, Lidia A. Bobrovnikova, Anna D. Temraleeva, Alexandra G. Markelova, David A. Gabrielyan, Maria A. Sinetova
Summary: Despite the large number of publications about Chlorella-like algae, their identification remains challenging due to their simplicity and ability to change their appearance. This study uses molecular techniques to understand the diversity of these algae and proposes a new genus, Neochlorella, with IPPAS C-1210 as the type species. Additionally, the study reassigns the strain C. thermophila ITBB HTA 1-65 to this new genus. The study also discusses the unique ultrastructural and physiological traits of the new species.
Review
Biology
Natalya Yuorieva, Maria Sinetova, Ekaterina Messineva, Irina Kulichenko, Artem Fomenkov, Olga Vysotskaya, Ekaterina Osipova, Angela Baikalova, Olga Prudnikova, Maria Titova, Alexander V. V. Nosov, Elena Popova
Summary: This review focuses on six genetic collections of algae, cyanobacteria, and plant materials maintained at the Institute of Plant Physiology of the Russian Academy of Sciences. These collections have been preserved since the 1950-1970s using in vitro and cryopreservation techniques. They play a significant role in research, biotechnological interventions, commercial application, and biodiversity conservation, and serve as a genetic basis for green biotechnologies.
Article
Biochemistry & Molecular Biology
Maria Breygina, Alexander Voronkov, Tatiana Ivanova, Ksenia Babushkina
Summary: Pollen grains are unique haploid organisms with special composition and structure. Angiosperms and gymnosperms share similar mechanisms for pollen germination but gymnosperms exhibit slower growth rates and reduced reliance on female tissues. The properties of pollen lipids, especially very-long-chain FAs, contribute to these features. By analyzing the absolute content and FA composition of pollen lipids, significant differences were found between species, with spruce pollen exhibiting a higher proportion of saturated and monoene FAs. Lily and tobacco also showed variations in the FA composition between pollen coat and gametophyte cells, with a lower unsaturation index in the coat. Moreover, the absolute content of lipids was highest in lily pollen. This study provides new insights into the FA changes during pollen germination in gymnosperms and angiosperms, and highlights the impact of H2O2 on spruce pollen germination.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
A. I. Solov'eva, A. Y. Stepanova, T. V. Ivanova, A. S. Voronkov, Yu. M. Panov
Summary: This study investigated the effects of hydrogen peroxide (H2O2) on hairy roots of Scutellaria baicalensis. It was found that 1 mM H2O2 acted as a signaling molecule, promoting growth and increasing flavones content, while 10 mM H2O2 inhibited growth, increased malondialdehyde content, and reduced baicalein content.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Pavel Pashkovskiy, Lyudmila Khalilova, Mikhail Vereshchagin, Alexander Voronkov, Tatiana Ivanova, Anatoliy A. Kosobryukhov, Suleyman I. Allakhverdiev, Vladimir D. Kreslavski, Vladimir V. Kuznetsov
Summary: This study investigated the effects of different light spectra on photosynthesis, photomorphogenesis, and gene expression in Marchantia polymorpha. The results showed that red light and blue light had distinct effects on gene transcription and gametophyte morphology, while far-red light led to reduced photosynthetic activity. The ultrastructure of chloroplasts in different tissues was also examined.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Maria Breygina, Dmitry Kochkin, Alexander Voronkov, Tatiana Ivanova, Ksenia Babushkina, Ekaterina Klimenko
Summary: This study investigated the composition of stigma exudates in tobacco and lily plants, and their effects on pollen germination. It was found that abscisic acid (ABA) was present in tobacco stigma exudate and had a stimulating effect on pollen germination. Analysis also showed differences in the fatty acid composition, with tobacco stigma exudate being more unsaturated compared to lily stigma exudate. Both exudates were found to contain squalene.
Article
Microscopy
T. Kh. Kumachova, A. V. Babosha, A. S. Ryabchenko, A. S. Voronkov
Summary: This study investigated the micromorphology, histochemistry, and fluorescence characteristics of colleters on leaf structures of Mespilus germanica L. Colleters were found on the tips of leaf blades and stipules, and less frequently on the petioles. They were multicellular secretory structures and their secretion process occurred during the active growth of leaf structures. Polysaccharides, phenolic substances, and lipids were detected in the colleters. The fluorescence spectrum showed a peak at 671-672 nm upon excitation at 405 and 473 nm.
