Article
Biochemistry & Molecular Biology
Sini Li, Yan Qu, Lihui Liu, Xue Zhang, Yan He, Chao Wang, Yufeng Guo, Li Yuan, Zixiao Ma, Hua Bai, Jie Wang
Summary: This study investigated the plasma exosomes' proteomic profiles in patients with lung cancer (LC) who had liver metastasis (LM) or brain metastasis (BM). Differentially expressed exosome-based proteins (DEEPs) were identified in LM and BM, and they were found to be involved in proteomic triggering cascade, ECM-receptor interaction, and the collagen-containing extracellular matrix. Potential biomarkers for the diagnosis of LM and BM were also suggested.
CELL AND BIOSCIENCE
(2023)
Article
Gastroenterology & Hepatology
Soo Young Cho, Heeyoun Hwang, Yun-Hee Kim, Byong Chul Yoo, Nayoung Han, Sun-Young Kong, Min-Jeong Baek, Kyung-Hee Kim, Mi Rim Lee, Jae Gwang Park, Sung-Sik Han, Woo Jin Lee, Charny Park, Jong Bae Park, Jin Young Kim, Sang-Jae Park, Sang Myung Woo
Summary: Through comprehensive analysis of genomic, transcriptomic, proteomic, and phosphoproteomic data from 102 iCC patients, three clinically supported subtypes were identified (stem-like, poorly immunogenic, and metabolism), and an organoid model was constructed for therapeutic testing. The ALDH1A1 inhibitor NCT-501 showed synergy with nanoparticle albumin-bound-paclitaxel in the organoid model for the stem-like subtype. Dysregulations in oncometabolites were associated with different clinical outcomes in the stem-like and metabolism subtypes. The poorly immunogenic subtype exhibited non-T-cell tumor infiltration. Integrated multiomics analysis reproduced the 3 subtypes and revealed heterogeneity in iCC.
Review
Medicine, Research & Experimental
Hongyuan Liang, Lingyun Zhang, Jian Rong
Summary: The incidence and mortality rates of lung cancer (LC) are increasing globally each year. LC is difficult to detect early and easily metastasizes and relapses. Currently, there is no effective cure for advanced metastatic LC, leading to poor prognosis. Exosomes, nano-sized extracellular vesicles produced by healthy or diseased cells, contain various functional molecules and play crucial roles in the occurrence and metastasis of many malignancies, including LC. Understanding the potential roles of exosomes in the initiation, invasion, and metastasis of LC and the underlying molecular mechanisms could contribute to improved early diagnosis and treatment.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Cell Biology
Hao Zhou, Xiaoyun He, Yuxiang He, Chunlin Ou, Pengfei Cao
Summary: Exosomes play crucial roles in a range of human diseases, especially malignant tumors, by transmitting cargo for intercellular communication. Exosomal circRNAs have the potential to regulate tumor cell metastasis and serve as valuable diagnostic biomarkers and therapeutic targets in cancer patients. The mechanism by which exosomal circRNAs modulate metastatic phenomena and the prospects of their clinical applications in tumor therapy are discussed in this review.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Oncology
Agathe Quesnel, Amy Broughton, George S. Karagiannis, Panagiota S. Filippou
Summary: Exosomes are a type of extracellular vesicles that play a role in cell-to-cell communication by transporting biological molecules. The proteolytic enzymes carried by exosomes have been found to be important in the development and progression of cancer. However, their targeting has not been fully explored in the context of personalized medicine.
CANCER AND METASTASIS REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Eleni-Myrto Trifylli, Anastasios G. Kriebardis, Evangelos Koustas, Nikolaos Papadopoulos, Sofia Vasileiadi, Sotirios P. Fortis, Vassilis L. Tzounakas, Alkmini T. Anastasiadi, Panagiotis Sarantis, Effie G. Papageorgiou, Ariadne Tsagarakis, Georgios Aloizos, Spilios Manolakopoulos, Melanie Deutsch
Summary: This review provides an overview of the emerging role of extracellular vesicles in cholangiocarcinogenesis and their potential as diagnostic and therapeutic tools.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Lushan Peng, Dan Wang, Yingying Han, Tao Huang, Xiaoyun He, Junpu Wang, Chunlin Ou
Summary: CAF-derived exosomes play a crucial role in tumorigenesis, tumor progression, and clinical characteristics of cancer patients, and have the potential to serve as diagnostic biomarkers and therapeutic targets in human cancers.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Jinyi Liu, Liwen Ren, Sha Li, Wan Li, Xiangjin Zheng, Yihui Yang, Weiqi Fu, Jie Yi, Jinhua Wang, Guanhua Du
Summary: Exosomes are cell-derived nanovesicles that play essential roles in intercellular communication, cancer progression, and drug delivery. They have potential applications in cancer diagnosis, treatment, and research.
ACTA PHARMACEUTICA SINICA B
(2021)
Editorial Material
Cardiac & Cardiovascular Systems
Chantal M. Boulanger, Xavier Loyer, Pierre-Michael Coly, Nicolas Amabile
Summary: This editorial discusses the impact of myocardial hypoxic stress on the release of functional extracellular vesicles.
EUROPEAN HEART JOURNAL
(2021)
Article
Cell Biology
Martina Morini, Federica Raggi, Martina Bartolucci, Andrea Petretto, Martina Ardito, Chiara Rossi, Daniela Segalerba, Alberto Garaventa, Alessandra Eva, Davide Cangelosi, Maria Carla Bosco
Summary: This study characterizes the specifically expressed exosome proteins in neuroblastoma (NB) patients for the first time, which are associated with tumor phenotype and disease stage. Some of these proteins show deregulated expression in NB patients and are mainly involved in tumor-associated pathways. Additionally, certain proteins can differentiate between high-risk and low-risk NB patients with high accuracy.
Review
Medicine, General & Internal
Angel Earle, Madison Bessonny, Josh Benito, Kun Huang, Hannah Parker, Emily Tyler, Brittany Crawford, Nabeeha Khan, Bridget Armstrong, Alexis Stamatikos, Sudha Garimella, Alyssa Clay-Gilmour
Summary: The early detection of chronic kidney disease is crucial, and there is growing interest in finding new biomarkers. Currently, there is no single effective biomarker, but there is evidence supporting the use of urinary exosomal microRNAs as biomarkers for kidney fibrosis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Manuel Ramirez-Garrastacho, Viktor Berge, Aija Line, Alicia Llorente
Summary: The study found that miRNAs can be used to differentiate ISUP grades of prostate cancer patients, with miR-186-5p and similar miRNAs showing consistent results in different cohorts, indicating the potential role of liquid biomarkers.
BRITISH JOURNAL OF CANCER
(2022)
Review
Oncology
Gianluca Rompianesi, Marcello Di Martino, Alex Gordon-Weeks, Roberto Montalti, Roberto Troisi
Summary: Cholangiocarcinoma (CCA) is a heterogeneous group of tumors with complex diagnosis, management, and poor prognosis. Liquid biopsy (LB) offers a less invasive and serially repeatable way to detect tumor biomarkers from fluid samples, providing real-time monitoring of tumor genetic profile or therapy response.
WORLD JOURNAL OF GASTROINTESTINAL ONCOLOGY
(2021)
Review
Pharmacology & Pharmacy
Dwaipayan Dey, Srestha Ghosh, Divya Mirgh, Siva Parsad Panda, Niraj Kumar Jha, Saurabh Kumar Jha
Summary: This review discusses the role of exosomes in prostate cancer and their association with male fertility.
DRUG DISCOVERY TODAY
(2023)
Article
Cell Biology
Yun Lu, Xiangyu Li, Kai Zhao, Yuanxin Shi, Zhengdong Deng, Wei Yao, Jianming Wang
Summary: This study identified novel protein kinases associated with cholangiocarcinoma (CCA) and demonstrated the effectiveness of the pan-inhibitor of the PKD family, 1-naphthyl PP1 (1-NA-PP1), in inhibiting the proliferation, migration, and invasion ability of CCA cells. These findings provide new therapeutic targets for CCA.
Article
Food Science & Technology
Kanokwan Nonthawong, Chantragan Srisomsap, Daranee Chokchaichamnankit, Jisnuson Svasti, Pharima Phiriyangkul
Summary: This study compared the proteins in fresh and powdered fish from skipjack tuna and Nile tilapia using proteomic approach. It identified potential allergens and discovered peptide markers for fish adulteration. Interestingly, the potential allergens in fish were similar to peptides from chicken powder and egg yolk powder.
Article
Biochemistry & Molecular Biology
Nittaya Suwannachuen, Kantinan Leetanasaksakul, Sittiruk Roytrakul, Narumon Phaonakrop, Siriwan Thaisakun, Peerapat Roongsattham, Chatchawan Jantasuriyarat, Nuttha Sanevas, Anchalee Sirikhachornkit
Summary: Photosynthetic organisms need light to survive, but excessive light can produce reactive oxygen species (ROS) and cause cell damage or death. Studying plants' responses to light can improve their growth and productivity. This study compared the responses of two field isolates of Chlamydomonas to high light stress and found that the light-tolerant strain managed ROS better and utilized palmelloid formation and cell aggregation to protect cells from excessive light.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Environmental
Pakakrong Sangsanoh, Sonthaya Chaiarwut, Chasuda Choipang, Jitti Niyompanich, Orawan Suwantong, Kriengsak Lirdprapamongkol, Jisnuson Svasti, Piyachat Chuysinuan, Supanna Techasakul, Pitt Supaphol
Summary: Semi-solid PVA-borax hydrogels containing CBD/β-CD inclusion complex were prepared for wound management. The inclusion complex was successfully prepared and loaded into PVA hydrogels. The hydrogels showed antibacterial capability against S. aureus and MRSA and improved antioxidant properties when CBD was added. The hydrogels were non-cytotoxic and suppressed NO production in cells. These findings highlight the potential biomedical application of these hydrogels.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2023)
Article
Oncology
Siriporn Keeratichamroen, Thiwaree Sornprachum, Lukana Ngiwsara, Narittira Ornnork, Jisnuson Svasti
Summary: The tumor microenvironment has a significant impact on therapeutic responses. 3D in vitro culture of cancer cells mimics the complex 3D arrangement of tumors in vivo and provides insights into the mechanisms of microenvironmental influences on cellular functions and therapeutic efficacy. This study demonstrates that 3D-cultured A549 cells exhibit dormant cell characteristics and increased resistance to anticancer agents, suggesting the importance of 3D culture systems in searching for novel chemotherapeutic agents and therapeutic targets for cancer treatment.
Article
Polymer Science
Piyachat Chuysinuan, Chalinan Pengsuk, Kriengsak Lirdprapamongkol, Thanyaluck Thanyacharoen, Supanna Techasakul, Jisnuson Svasti, Patcharakamon Nooeaid
Summary: This study developed a wound dressing made of natural- and biowaste-derived biopolymers loaded with turmeric extract, which has antibacterial, antioxidant, and anti-inflammatory activities. The dressing showed inhibitory effects on bacterial growth due to the controlled release of the turmeric extract. It also exhibited antioxidant activity by scavenging radicals and demonstrated anti-inflammatory effects by inhibiting nitric oxide production. This study suggests that the dressing has potential for wound healing.
Article
Polymer Science
Seubsakul Phuegyod, Sasivimon Pramual, Nungnit Wattanavichean, Supasuda Assawajaruwan, Taweechai Amornsakchai, Panithi Sukho, Jisnuson Svasti, Rudee Surarit, Nuttawee Niamsiri
Summary: In this study, 3D porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content were fabricated and their in vitro proliferation of dental cells for tissue engineering application was evaluated. The results showed that the P(HB-50HV) scaffold exhibited excellent cell compatibility and could be applied as a biomaterial for periodontal tissue engineering and stem cell applications.
Article
Multidisciplinary Sciences
Mashima Naksawat, Chosita Norkaew, Kantorn Charoensedtasin, Sittiruk Roytrakul, Dalina Tanyong
Summary: This study revealed the anti-leukemic effects and underlying mechanisms of menthol in NB4 and Molt-4 leukemic cell lines, including induction of apoptosis and autophagy through activating the caspase cascade, regulating BAX and p53/MDM2, and modulating autophagy via the ATG3/mTOR signaling pathway.
Article
Food Science & Technology
Krittika Srisuksai, Kongphop Parunyakul, Pitchaya Santativongchai, Narumon Phaonakrop, Sittiruk Roytrakul, Phitsanu Tulayakul, Wirasak Fungfuang
Summary: Crocodile oil is rich in monounsaturated fatty acids and polyunsaturated fatty acids, which have antioxidant and cognitive effects. This study investigated the effect of crocodile oil on antioxidant activity and cognitive function in rats. The results showed that crocodile oil significantly decreased triglyceride levels and had stronger free radical scavenging ability compared to olive oil. It also improved memory function in rats without causing a decline in cognitive function. Therefore, crocodile oil can be considered as an alternative dietary oil with hypolipidemic and antioxidant effects.
Article
Biochemistry & Molecular Biology
Benya Nontaleerak, Chatchakorn Eurtivong, Churat Weeraphan, Hansuk Buncherd, Daranee Chokchaichamnankit, Chantragan Srisomsap, Jisnuson Svasti, Rojana Sukchawalit, Skorn Mongkolsuk
Summary: This study found that oxidation of C104 in the NieR protein under NaOCl stress leads to the formation of a disulfide-linked dimer, altering the protein's conformation and thereby abolishing its DNA-binding ability and repressing the expression of target genes.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Santhasiri Orrapin, Patcharawadee Thongkumkoon, Sasimol Udomruk, Sutpirat Moonmuang, Songphon Sutthitthasakul, Petlada Yongpitakwattana, Dumnoensun Pruksakorn, Parunya Chaiyawat
Summary: Circulating tumor cells (CTCs) play a crucial role in hematogenous dissemination and studying them is challenging due to their rarity and heterogeneity. Single-cell RNA sequencing (scRNA-seq) technology provides insights into CTC biology and helps identify potential therapeutic targets. This review comprehensively describes the current research on transcriptomic analysis of CTCs using scRNA-seq and highlights the workflow and bioinformatic tools involved.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Nilobon Jeanmard, Rassanee Bissanum, Hutcha Sriplung, Sawanya Charoenlappanit, Sittiruk Roytrakul, Raphatphorn Navakanitworakul
Summary: This study conducted a proteomic analysis comparing uEVs in healthy individuals and breast cancer patients, revealing differential protein expression and identifying potential biomarkers for breast cancer screening and diagnosis.
Article
Multidisciplinary Sciences
Pakorn Ruengket, Sittiruk Roytrakul, Daraka Tongthainan, Kanokwan Taruyanon, Bencharong Sangkharak, Paviga Limudomporn, Mongkol Pongsuchart, Chanya Udom, Wirasak Fungfuang
Summary: The number of patients infected with simian malaria is increasing in many countries of Southeast Asia and South America. This study identifies differentially expressed proteins in the serum of wild stump-tailed macaques with varying malarial infection status. The findings provide important insights into the understanding of malarial infection and host response, which can be crucial for diagnostic tools, medication development, and therapies.
Article
Multidisciplinary Sciences
Sittiruk Roytrakul, Pongsathorn M. Sangprasert, Janthima M. Jaresitthikunchai, Narumon M. Phaonakrop, Teerakul M. Arpornsuwan
Summary: This study aimed to find peptidome-based biomarkers of multidrug-resistant Neisseria gonorrhoeae using mass spectrometry and liquid chromatography. The peptide barcode database of multidrug-resistant N. gonorrhoeae was generated from 93 isolates from Thailand. The results showed that these isolates could be classified into five groups, including azithromycin resistance, ciprofloxacin resistance, ciprofloxacin and penicillin resistance, ciprofloxacin and tetracycline resistance, and ciprofloxacin, penicillin and tetracycline resistance. Comparative analysis of the peptidomes of these groups revealed nine peptides highly expressed in the azithromycin resistance group, which played a crucial role in pathways associated with antibiotic resistance.
Article
Multidisciplinary Sciences
Chadaporn Chaiden, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sittiruk Roytrakul, Anusak Kerdsin, Suphachai Nuanualsuwan
Summary: In this study, the peptidomes of highly virulent SS2 and less prevalent serotypes SS14, SS18, and SS19 of Streptococcus suis were analyzed. Six serotype-specific peptides were found in SS2 and their high expression may be related to bacterial cellular stability and cell wall formation. These virulence-related peptides could enhance the competitiveness of SS2 with other coexistences in specific conditions. Further in vivo studies are needed to confirm the pathogenic roles of these identified peptides.
Article
Multidisciplinary Sciences
Pornsuda Chawengrum, Natthaorn Luepongpatthana, Sanit Thongnest, Jitnapa Sirirak, Jutatip Boonsombat, Kriengsak Lirdprapamongkol, Siriporn Keeratichamroen, Patcharin Kongwaen, Phreeranat Montatip, Prasat Kittakoop, Jisnuson Svasti, Somsak Ruchirawat
Summary: Anticopalic acid (ACP), together with its derivatives, showed cytotoxic activity against cancer cell lines. Compound 4p exhibited the highest activity against leukemia cell lines, while compound 4l showed activity against triple-negative breast cancer cell line MDA-MB-231. Mechanistic studies revealed that compound 4l induced cell death through non-apoptotic regulated cell death and decreased the phosphorylation of FAK protein.
SCIENTIFIC REPORTS
(2023)
