Article
Materials Science, Multidisciplinary
Pengkai Wu, Shuai Yin, Tianqing Liu, Dan Ding, Kaikai Wang
Summary: By incorporating different functional ligands and linked reductive disulfides into low-molecular-weight PEI 1.8k, this study constructed reductive-responsive PEI-X-SS-X-PEI copolymers to enhance siRNA transfection efficiency. The strategy, named building-block crosslinking, showed a simple and smart way to synthesize new materials and improve gene delivery systems.
SCIENCE CHINA-MATERIALS
(2021)
Article
Polymer Science
Jixiang Ni, Matteo Lanzi, David H. Lamparelli, Arjan W. Kleij
Summary: A series of bicyclic carbonates with different functionalities have been prepared through various chemical reactions, which can be polymerized to obtain polycarbonates with diverse properties. This method provides an effective approach to synthesize functional polymer materials.
Article
Thermodynamics
Lipeng Liu, Ying Lu, Mingze Xia, Bo Wang, Yi Cheng, Zhiyong Wei
Summary: Aliphatic polycarbonates (APCs) have been synthesized using dimethyl carbonate (DMC) and different lengths of aliphatic diols, showing increased flexibility and crystallization with more methylene groups in the repeating units. However, the rigidity of the ester group affected the strength and Young's modulus of APCs, which decreased and then increased. Brill transition increased the distance between intermolecular chains, affecting the barrier properties of poly(octamethylene carbonate).
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2023)
Article
Multidisciplinary Sciences
Yuan Rui, David R. Wilson, Stephany Y. Tzeng, Hannah M. Yamagata, Deepti Sudhakar, Marranne Conge, Cynthia A. Berlinicke, Donald J. Zack, Anthony Tuesca, Jordan J. Green
Summary: Nanoparticle-based mRNA therapeutics show great promise, but cellular internalization and endosomal escape pose challenges for cytosolic delivery. Researchers developed a dual nanoparticle uptake and endosomal disruption assay, which can detect cellular internalization and endosomal disruption. The study found that this assay has higher predictive capacity for mRNA delivery compared to conventional polymer and nanoparticle characteristics.
Article
Nanoscience & Nanotechnology
Michael Karimov, Marion Schulz, Tim Kahl, Sandra Noske, Malgorzata Kubczak, Ines Gockel, Rene Thieme, Thomas Buech, Anja Reinert, Maksim Ionov, Maria Bryszewska, Heike Franke, Ute Kruegel, Alexander Ewe, Achim Aigner
Summary: This study demonstrates that tyrosine-modified linear PEIs significantly enhance the delivery and knockdown efficacy of siRNA in vitro, particularly LP10Y. This approach shows good biocompatibility and therapeutic anti-tumor effects in cell lines, mouse xenograft and patient-derived xenograft models.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jixian Luo, Jiangfeng Chen, Yan Liu, Yongji He, Wenjuan Dong
Summary: The modification of low-molecular-weight chitosan with arginine resulted in the successful creation of arginine-chitosan nanoparticles. These nanoparticles were able to encapsulate and deliver siRNA safely to leukemia cells, making them a potential tool for future therapies targeting specific genes. This study identified arginine-chitosan nanoparticles for the first time and explored their ability to deliver Rhoa siRNA into T-cell acute lymphoblastic leukemia cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Panagiotis Bexis, Julien De Winter, Maria C. Arno, Olivier Coulembier, Andrew P. Dove
Summary: The synthesis of well-defined propargyl-functional aliphatic polycarbonates is achieved using commercially available or readily made, shelf-stable organocatalysts, resulting in homopolymers with low dispersities and end-group fidelity, and demonstrating the versatility of the system through the synthesis of telechelic copolymers and block copolymers with molar mass up to 40 kDa.
MACROMOLECULAR RAPID COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Krishnapriya Syama, Zygmunt J. Jakubek, Sam Chen, Josh Zaifman, Yuen Yi C. Tam, Shan Zou
Summary: This study evaluated the toxicity of six lipid-based formulations on cells and found that anionic, cationic, and neutral lipid formulations were non-toxic. These findings suggest that these lipid-based formulations have potential as drug carriers.
SCIENTIFIC REPORTS
(2022)
Article
Materials Science, Multidisciplinary
Zhipeng Hou, Wei Xu, Siwen Chen, Jing Guo, Peng Li, Jianshe Hu, Liqun Yang, Jing Chen
Summary: In this study, new biodegradable contraceptive implants were developed using the UV irradiation molding method. The implants showed good degradation characteristics, with no acidic degradation products and excellent form stability. They also exhibited good biocompatibility and biosafety. The implants achieved stable and sustained release of the model drug LNG in female rats, providing a new option for birth control needs.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Oncology
Ilaria Cocchiararo, Melanie Cornut, Hadrien Soldati, Alessandro Bonavoglia, Perrine Castets
Summary: C2C12 cells are commonly used in muscle research, but transfecting these cells is challenging. This study compared the efficiency of five commercial transfection reagents in C2C12 cells and identified some low-cost alternatives to the current gold standard.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Chemistry, Physical
Yijia Yu, Shengjie Liu, Wenjuan Xia, Zhichen Zhu, Wenjin Wang, Chuanjiang Zhou, Zhaoqiang Wu, Hong Chen
Summary: A novel self-polishing antibacterial surface was developed by surface-initiated ring-opening grafting polymerization and sulfur(IV)-fluorine exchange postclick modification. The functionalized surface exhibited good biocompatibility, enzymatic biodegradable properties, and excellent bactericidal properties.
APPLIED SURFACE SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Wen Song, Qingyuan Ye, Zhe Li, Yajie Hou, Lina Niu, Zhiwei Ma, Chuanxu Yang
Summary: This study reveals the significant impact of transfection medium on chitosan-mediated siRNA delivery, with even disturbed physical parameters of nanoparticles still achieving high knockdown efficiency.
MATERIALS & DESIGN
(2022)
Article
Polymer Science
Matthew R. Bockman, Rishad J. Dalal, Ramya Kumar, Theresa M. Reineke
Summary: This study presents an improved synthetic route to incorporate a GalNAc-derived monomer into polymers targeting hepatocytes, showing good performance in terms of toxicity, uptake, and transfection efficiency against liver cells.
Article
Environmental Sciences
Xue Shi, Yu-Qing Wang, Yue-Ming Yang, Yi-Feng Li
Summary: This study investigated the expression of two deiodinase genes, McDx and McDy, in the larval stages of Mytilus coruscus. The results showed that these two genes are essential for larval development and knockdown of their expression significantly reduced larval metamorphosis. The study suggests a synergistic action of adrenergic and thyroid hormones in larval development and metamorphic transition in M. coruscus.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Polymer Science
Beste Cagdas Tunali, Omer Akturk, Durukan Sahingoz, Mustafa Turk, Ayten Celebi Keskin
Summary: In this study, polyethyleneimine-capped gold nanoparticles (PEI-AuNPs) were synthesized using the pulsed laser ablation in liquid (PLAL) technique, and then combined with Bombyx mori silk fibroin (SF) to form nanocomposites (PEI-SF-AuNPs). The biocompatibility of PEI-AuNPs and PEI-SF-AuNPs in breast cancer cells MCF-7 and MDA-MB231 were tested, and it was found that PEI-AuNP1 showed the least cytotoxicity. Therefore, PEI-AuNP1 nanocomposites can be suggested as siRNA carriers in gene-based breast cancer therapies.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Oncology
Antoine Bernard, Christophe Hibos, Corentin Richard, Etienne Viltard, Sandy Chevrier, Sophie Lemoine, Josephine Melin, Etienne Humblin, Romain Mary, Theo Accogli, Fanny Chalmin, Melanie Bruchard, Paul Peixoto, Eric Hervouet, Lionel Apetoh, Francois Ghiringhelli, Frederique Vegran, Romain Boidot
Summary: The tumor microenvironment (TME) influences immune cell activity by increasing alternative splicing events, leading to the expression of IRF1 Delta 7 in Th1 cells and subsequently decreasing IFN gamma secretion. Inhibiting the expression of IRF1 Delta 7 could enhance Th1 antitumor responses.
CANCER IMMUNOLOGY RESEARCH
(2021)
Article
Chemistry, Analytical
Elodie Dumont, Charlotte De Bleye, Gilles Rademaker, Laureen Coic, Julie Horne, Pierre-Yves Sacre, Olivier Peulen, Philippe Hubert, Eric Ziemons
Summary: A new methodology combining solid SERS substrates, a protein filtering device, and a handheld Raman analyzer enables near real-time, non-invasive, and non-destructive SERS monitoring of biological samples. This method has shown promising versatility and the capability to reduce cellular stress and contamination hazards.
Article
Oncology
Rafael S. Martinez, Mark J. Salji, Linda Rushworth, Chara Ntala, Giovanny Rodriguez Blanco, Ann Hedley, William Clark, Paul Peixoto, Eric Hervouet, Elodie Renaude, Sonia H. Y. Kung, Laura C. A. Galbraith, Colin Nixon, Sergio Lilla, Gillian M. Mackay, Ladan Fazli, Luke Gaughan, David Sumpton, Martin E. Gleave, Sara Zanivan, Arnaud Blomme, Hing Y. Leung
Summary: This study identified SLFN5 as a novel regulator of the LAT1 amino acid transporter in castration-resistant prostate cancer (CRPC), contributing to mTORC1 activity. High expression of SLFN5 in CRPC tumors was correlated with poor patient outcome, indicating its clinical relevance as a potential target for CRPC treatment.
Review
Immunology
Elodie Renaude, Marie Kroemer, Christophe Borg, Paul Peixoto, Eric Hervouet, Romain Loyon, Olivier Adotevi
Summary: The review discusses the role of CD4(+) Th cells in anticancer immunity and emphasizes the importance of epigenetics. Research describes how epigenetic factors regulate the differentiation and recruitment of CD4(+) T cells. Furthermore, the potential of using epigenetic drugs to enhance anticancer immunotherapy is explored.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Camille Lachat, Paul Peixoto, Eric Hervouet
Summary: Epithelial to mesenchymal transition (EMT) is a process that allows cells to acquire a mesenchymal phenotype, first studied in embryonic development. It has now become an important field in cancer research, as it has been shown to play various roles in cancer progression.
Article
Biochemistry & Molecular Biology
Seng Chuan Tang, Quentin Lion, Olivier Peulen, Philippe Chariot, Arnaud Lavergne, Alice Mayer, Paula Allepuz Fuster, Pierre Close, Sebastian Klein, Alexandra Florin, Reinhard Buettner, Ivan Nemazanyy, Kateryna Shostak, Alain Chariot
Summary: COP1 acts as an oncogenic E3 ligase by promoting ERα signaling but also acts as a tumor suppressor candidate by preventing EMT, reflecting a dual role of COP1 in breast cancer that has sparked widespread interest in the academic community.
Article
Multidisciplinary Sciences
Amandine Duchemin, Tina O'Grady, Sarah Hanache, Agnes Mereau, Marc Thiry, Ludivine Wacheul, Catherine Michaux, Eric Perpete, Eric Hervouet, Paul Peixoto, Felix G. M. Ernst, Yann Audic, Franck Dequiedt, Denis L. J. Lafontaine, Denis Mottet
Summary: TFIP11, a human homolog of the yeast spliceosome disassembly factor Ntr1, is essential for U6 snRNA 2'-O-methylation and U4/U6.U5 tri-snRNP assembly, independently of its known partner DHX15. This reveals a previously unrecognized function of TFIP11 in spliceosome assembly regulation in human cells.
NATURE COMMUNICATIONS
(2021)
Article
Polymer Science
Joanna Jaworska, Michal Sobota, Malgorzata Pastusiak, Michal Kawalec, Henryk Janeczek, Piotr Rychter, Kamila Lewicka, Piotr Dobrzynski
Summary: This work presents the results of research on the preparation of bioresorbable functional polyestercarbonates containing side carboxyl groups. The simplified one-step method allows obtaining copolymers with all carbonate units containing carboxyl groups, avoiding the issue of heavy metals residue from the traditional method.
Review
Chemistry, Analytical
Hussam A. AL Saoud, Myroslav Sprynskyy, Reza Pashaei, Michal Kawalec, Pawel Pomastowski, Boguslaw Buszewski
Summary: Diatomaceous biosilica is a unique and renewable material with various applications in different industries due to its chemical inertness, biocompatibility, and high stability.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Dawei Chen, Ivan Nemazanyy, Olivier Peulen, Kateryna Shostak, Xinyi Xu, Seng Chuan Tang, Caroline Wathieu, Silvia Turchetto, Sylvia Tielens, Laurent Nguyen, Pierre Close, Christophe Desmet, Sebastian Klein, Alexandra Florin, Reinhard Buttner, Georgios Petrellis, Benjamin Dewals, Alain Chariot
Summary: This study reveals the involvement of translational reprogramming in macrophage polarization, with a focus on the role of the Elongator complex subunit Elp3. Elp3 expression is regulated by different activating signals, with M1 signals downregulating its expression and M2 signals upregulating it. The metabolic reprogramming associated with M2 macrophage polarization is dependent on Elp3 and the translation of specific genes. Additionally, Elp3 plays a role in mTORC2 activation and the initiation of Wnt-driven tumor in the intestine by maintaining a pool of M2-like tumor-associated macrophages.
Article
Oncology
Gilles Rademaker, Brunella Costanza, Sebastien Pyr Dit Ruys, Raphael Peiffer, Ferman Agirman, Naima Maloujahmoum, Didier Vertommen, Andrei Turtoi, Akeila Bellahcene, Vincent Castronovo, Olivier Peulen
Summary: Paladin is identified as a potential biomarker for colon cancer liver metastases, and its depletion inhibits colon cancer cell migration and metastasis.
Article
Biochemistry & Molecular Biology
Gilles Rademaker, Yasmine Boumahd, Raphael Peiffer, Sandy Anania, Tom Wissocq, Maude Liegeois, Geraldine Luis, Nor Eddine Sounni, Ferman Agirman, Naima Maloujahmoum, Pascal De Tullio, Marc Thiry, Akeila Bellahcene, Vincent Castronovo, Olivier Peulen
Summary: The pharmacological compound WJ460, targeting myoferlin, triggers mitophagy and ROS accumulation, leading to lipid peroxidation and apoptosis-independent cell death. Inhibitors of mitophagy and iron chelators can suppress myoferlin-induced ROS production and restore cell growth. Additionally, a synergic effect exists between ferroptosis inducers and WJ460.
Article
Engineering, Biomedical
Magdalena Lepicka, Magdalena Rodziewicz, Michal Kawalec, Klaudia Nowicka, Yurii Tsybrii, Krzysztof Jan Kurzydlowski
Summary: The effect of introducing diatomaceous earth as a filler in a glass-ionomer cement matrix was investigated. The results showed that diatomaceous earth particles are uniformly distributed in the matrix and can enhance surface microhardness and roughness, but have no effect on wear performance or compressive strength.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Review
Oncology
Raphael Peiffer, Yasmine Boumahd, Charlotte Gullo, Rebekah Crake, Elisabeth Letellier, Akeila Bellahcene, Olivier Peulen
Summary: Cancer-associated fibroblasts (CAFs) play a critical role in pancreatic cancer by influencing cancer cell metabolism through various mechanisms. CAF heterogeneity is characteristic of the pancreatic tumor microenvironment. Environmental changes in pH, oxygenation, and nutrient availability are heavily driven by stromal CAFs. The bidirectional crosstalk between CAFs and cancer cells promotes tumor aggressiveness and adaptation to external stress. This review summarizes the diversity of CAF subtypes, the metabolic crosstalk between CAFs and tumor cells, and the role of CAF-driven signaling, desmoplasia, and acidosis in cancer cell metabolism, highlighting potential therapeutic strategies.
Article
Cell Biology
Rebekah Crake, Imene Gasmi, Jordan Dehaye, Fanny Lardinois, Raphael Peiffer, Naima Maloujahmoum, Ferman Agirman, Benjamin Koopmansch, Nicky D'Haene, Oier Azurmendi Senar, Tatjana Arsenijevic, Frederic Lambert, Olivier Peulen, Jean-Luc Van Laethem, Akeila Bellahcene
Summary: PDAC patients with elevated levels of oncometabolite methylglyoxal (MG) and glyoxalase 1 (GLO1) present with poor prognosis. PDAC cells resistant to gemcitabine show increased glycolysis and MG stress compared to parental cells. Induction of MG stress and heat shock response (HSR) activation by gemcitabine can be efficiently reversed with potent MG scavengers, improving the outcomes of gemcitabine therapy in PDAC.
