Article
Cell Biology
Danai Georgiadou, Souad Boussata, Remco Keijser, Dianta A. M. Janssen, Gijs B. Afink, Marie van Dijk
Summary: Mutations in the LINC-HELLP non-coding RNA (HELLPAR) are associated with familial forms of the pregnancy-specific HELLP syndrome, impacting EVT differentiation and the binding of RNA splicing complex proteins PCBP1, PCBP2, and YBX1 to LINC-HELLP. Knockdown of PCBP2, and to a lesser extent YBX1 and PCBP1, leads to failure of EVTs to differentiate towards an invasive phenotype. PCBP2 silencing alters splicing for genes related to cellular assembly, organization, function, maintenance, growth, proliferation, and the cell cycle, indicating its crucial role in EVT differentiation mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Robert Morey, Omar Farah, Sampada Kallol, Daniela F. Requena, Morgan Meads, Matteo Moretto-Zita, Francesca Soncin, Louise C. Laurent, Mana M. Parast
Summary: During pregnancy, the EVT invades and remodels maternal blood vessels to ensure blood supply to the placenta, exhibiting polyploidy and senescence in early gestation. Comprehensive profiling of genomic organization and transcriptome shows that both first trimester and term EVT undergo senescence, are primarily tetraploid, and lack high rates of copy number variations. Genes and pathways related to senescence and polyploidy are important for normal EVT differentiation and maturation, with a key role identified for the unfolded protein response.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Cassandra Meakin, Emily S. Barrett, Lauren M. Aleksunes
Summary: The migration and invasion of extravillous trophoblasts are crucial for proper placenta and fetus development during pregnancy. Environmental chemicals and pharmaceuticals can disrupt trophoblast migration and may lead to adverse pregnancy outcomes.
REPRODUCTIVE TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Kaela M. Varberg, Khursheed Iqbal, Masanaga Muto, Mikaela E. Simon, Regan L. Scott, Keisuke Kozai, Ruhul H. Choudhury, John D. Aplin, Rebecca Biswell, Margaret Gibson, Hiroaki Okae, Takahiro Arima, Jay L. Vivian, Elin Grundberg, Michael J. Soares
Summary: Invasive trophoblast cells play a crucial role in placental development and pregnancy outcomes. ASCL2 is identified as a key regulator of invasive trophoblast cell lineage development in humans and rats. ASCL2 deficiency impairs EVT cell differentiation and impacts placental morphogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemical Research Methods
Yong Pu, Jeremy Gingrich, Almudena Veiga-Lopez
Summary: Placental trophoblast cell invasion into the maternal uterus is a crucial and complex event in the formation of the maternal-fetal interface. A 3D PDMS microfluidic platform has been developed to accurately evaluate trophoblast invasion, allowing for real-time monitoring, imaging, and collection of invasive cells. This platform provides insights into pathways regulating trophoblast invasion and is useful for toxicological screening.
Article
Obstetrics & Gynecology
Lufen Gao, Hui Chen, Jing Liu, Minghua Wang, Fangfang Lin, Guang Yang, Gendie E. Lash, Ping Li
Summary: In cesarean scar pregnancy, the implantation site displays increased fibrosis and disrupted myometrium, while the non-scar implantation sites have specific features of local decidua. The differentiation process of the decidua is important for the management and avoidance of consequences in cesarean scar pregnancy.
ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA
(2022)
Article
Toxicology
Cassandra Meakin, Christine Kim, Thomas Lampert, Lauren M. Aleksunes
Summary: Migration and invasion of extravillous trophoblasts into the uterus are crucial for placenta formation, and disruption of these processes can lead to adverse pregnancy outcomes. A novel, high-throughput method for screening chemicals that can alter human trophoblast migration was developed. This method involves culturing trophoblast cells in migration plates and tracking their movement into a cell-free detection zone. The method was validated using known chemicals and tested on a panel of environmental chemicals. This real-time method has potential for screening placental toxicants.
TOXICOLOGY LETTERS
(2023)
Article
Cell Biology
Jiaye Chen, Tinglin Song, Sizhu Yang, Qingxue Meng, Xiaoyu Han, Ze Wu, Jung-Chien Cheng, Lanlan Fang
Summary: This study aimed to investigate the effect of GDF-8 on invasion and MMP2 expression in human EVT cells, and found that Snail is a key factor in GDF-8-induced MMP2 expression and cell invasion.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Cell Biology
Eliza Grbac, Teresa So, Swati Varshney, Nicholas Williamson, Evdokia Dimitriadis, Ellen Menkhorst
Summary: Research suggests that prednisolone affects the expression of cytokines and protein production in human endometrial stromal fibroblasts (hESF), altering their regulation of trophoblast cells. Decidualization of hESF in the presence of prednisolone enhances trophoblast cell growth and influences the expression of cell motility genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yunali Ashar, Qiuxu Teng, John N. D. Wurpel, Zhe-Sheng Chen, Sandra E. Reznik
Summary: In this study, it was found that palmitic acid increases MRP1-mediated folate efflux in placental extravillous trophoblasts, thereby interfering with their role in early placental vascular remodeling.
Article
Cell Biology
Asli Ozmen, Ozlem Guzeloglu-Kayisli, Selcuk Tabak, Xiaofang Guo, Nihan Semerci, Chinedu Nwabuobi, Kellie Larsen, Ali Wells, Asli Uyar, Sefa Arlier, Ishani Wickramage, Hasan Alhasan, Hana Totary-Jain, Frederick Schatz, Anthony O. Odibo, Charles J. Lockwood, Umit A. Kayisli
Summary: In preeclampsia, the competition binding of IL-6 and IL-11 to gp130 impairs the function and morphology of extravillous trophoblast cells (EVT), and the reduced expression of ISG15 is associated with this effect.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Orsolya Oravecz, Andrea Balogh, Roberto Romero, Yi Xu, Kata Juhasz, Zsolt Gelencser, Zhonghui Xu, Gaurav Bhatti, Roger Pique-Regi, Balint Peterfia, Petronella Hupuczi, Ilona Kovalszky, Padma Murthi, Adi L. Tarca, Zoltan Papp, Janos Matko, Nandor Gabor Than
Summary: Proteoglycan macromolecules, specifically syndecan-1, play crucial roles in various physiological processes during placenta development and healthy pregnancy. Dysregulated expression of proteoglycans, including syndecan-1, has been observed in pregnancy complications such as pre-eclampsia and fetal growth restriction. Further research is needed to explore the potential diagnostic and therapeutic applications of proteoglycans in complicated pregnancies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Tae Yokouchi-Konishi, Yongjie Liu, Liping Feng
Summary: Proper extravillous trophoblast invasion is important for normal placenta development and pregnancy. This study reveals that attenuation of progesterone receptor membrane component 2 promotes cell proliferation, invasion, and angiogenesis in extravillous trophoblasts by activating hypoxia-inducible factor 1 alpha signaling. This discovery provides new insights into the mechanisms of trophoblast invasion.
BIOLOGY OF REPRODUCTION
(2023)
Article
Peripheral Vascular Disease
Rebekka Vogtmann, Jacqueline Heupel, Florian Herse, Mahsa Matin, Henning Hagmann, Ivo Bendix, Kristin Kraeker, Ralf Dechend, Elke Winterhager, Rainer Kimmig, Angela Koeninger, Alexandra Gellhaus
Summary: By overexpressing hsFLT1 in mice, the study found that circulating maternal hsFLT1 is sufficient to induce typical maternal preeclampsia-like symptoms, impairing spiral artery remodeling independently from the fetoplacental compartment.
Article
Toxicology
Shoko Ogushi, Tsuyoshi Nakanishi, Tomoki Kimura
Summary: Cadmium inhibits trophoblast differentiation into extravillous cytotrophoblasts, leading to suppressed placental formation and a potential increase in the risks of gestational hypertension and early preterm birth.
TOXICOLOGICAL SCIENCES
(2023)
Article
Cell Biology
Hannah Lake, Kate Rainczuk, Evdokia Dimitriadis
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
(2019)
Article
Oncology
Michelle Van Sinderen, Meaghan Griffiths, Ellen Menkhorst, Keith Niven, Evdokia Dimitriadis
Article
Biochemistry & Molecular Biology
Lisanne George, Amy Winship, Kelli Sorby, Evdokia Dimitriadis, Ellen Menkhorst
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Peripheral Vascular Disease
Ellen Menkhorst, Wei Zhou, Leilani L. Santos, Sarah Delforce, Teresa So, Kate Rainczuk, Hannah Loke, Argyro Syngelaki, Swati Varshney, Nicholas Williamson, Kirsty Pringle, Morag J. Young, Kypros H. Nicolaides, Yves St-Pierre, Eva Dimitriadis
Article
Medicine, General & Internal
Evdokia Dimitriadis, Ellen Menkhorst, Shigeru Saito, William H. Kutteh, Jan J. Brosens
NATURE REVIEWS DISEASE PRIMERS
(2020)
Article
Cell Biology
Eliza Grbac, Teresa So, Swati Varshney, Nicholas Williamson, Evdokia Dimitriadis, Ellen Menkhorst
Summary: Research suggests that prednisolone affects the expression of cytokines and protein production in human endometrial stromal fibroblasts (hESF), altering their regulation of trophoblast cells. Decidualization of hESF in the presence of prednisolone enhances trophoblast cell growth and influences the expression of cell motility genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Niloufar Ansari, Tsuyoshi Isojima, Blessing Crimeen-Irwin, Ingrid J. Poulton, Narelle E. McGregor, Patricia W. M. Ho, Mark R. Forwood, Christopher S. Kovacs, Evdokia Dimitriadis, Jonathan H. Gooi, T. John Martin, Natalie A. Sims
Summary: PTHrP is a pleiotropic regulator of tissue homeostasis, with significant impact on bone development. The study found that parental genotype plays a crucial role in influencing bone size and strength in offspring under the same experimental conditions, indicating a maternal influence on skeletal size.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Article
Developmental Biology
Wei Zhou, Ellen Menkhorst, Evdokia Dimitriadis
Summary: This study identified the expression pattern of CLIC4 in the placenta and decidua, and revealed its association with trophoblast cell function and TGF beta signaling. Knockdown of CLIC4 led to increased cell adhesion, migration, and invasion, along with alterations in the expression of downstream targets of the TGF beta signaling pathway. These findings indicate an inhibitory role for CLIC4 in regulating trophoblast migration and invasion.
Article
Developmental Biology
Siena Barton, Wei Zhou, Leilani L. Santos, Ellen Menkhorst, Guannan Yang, Wan Tinn Teh, Catarina Ang, Tarana Lucky, Evdokia Dimitriadis
Summary: miR-23b-3p expression is increased in the fertile endometrium during receptivity. This study investigates its role in endometrial adhesion and its downstream targets.
Article
Medicine, Research & Experimental
Meaghan J. Griffiths, Sarah A. . Marshall, Fiona L. . Cousins, Lauren R. . Alesi, Jordan Higgins, Saranya Giridharan, Urooza C. . Sarma, Ellen Menkhorst, Wei Zhou, Alison S. . Care, Jacqueline F. . Donoghue, Sarah J. . Holdsworth-Carson, Peter A. W. . Rogers, Evdokia Dimitriadis, Caroline E. Gargett, Sarah A. Robertson, Amy L. Winship, Karla J. Hutt
Summary: Female cancer survivors are at a higher risk of infertility due to the damaging effects of chemotherapy and radiotherapy on the ovaries. However, the effects of cancer treatments on the uterus have been understudied. This study shows that total-body gamma-irradiation (TBI) damages the uterus, leading to compromised pregnancy success. The pro-apoptotic protein Puma is identified as a key mechanism in TBI-induced uterine damage, suggesting that inhibiting PUMA may be a potential fertility preservation strategy.
Review
Developmental Biology
Sarah J. Holdsworth-Carson, Ellen Menkhorst, Jacqueline A. Maybin, Anna King, Jane E. Girling
Summary: This review focuses on the complex changes in the female reproductive tract during the menstrual cycle and explores reproductive tract abnormalities associated with the cycle. Women and people who menstruate in high-income countries can expect to experience around 450 menstrual cycles between menarche and menopause. The primary function of the menstrual cycle is to prepare the reproductive system for pregnancy, and its end triggers menstruation. This paper discusses the physiological processes involved in uterine cyclicity in humans and relevant mammals, highlighting knowledge gaps and the impact of reproductive tract and uterine cycle perturbations on health and fertility.
MOLECULAR HUMAN REPRODUCTION
(2023)
Meeting Abstract
Obstetrics & Gynecology
Ellen Menkhorst, Wei Zhou, Teresa So, Leilani Santos, Jian-Guo Zhang, Argyro Syngelaki, Kypros Nicolaides, Yves St-Pierre, Eva Dimitriadis
REPRODUCTIVE SCIENCES
(2020)
Meeting Abstract
Obstetrics & Gynecology
Ellen Menkhorst, Kate Rainczuk, Eva Dimitriadis
REPRODUCTIVE SCIENCES
(2019)
