Article
Biochemistry & Molecular Biology
Guojun Zhang, Guoliang Zheng, Fengli Jiang, Tianyi Wu, Lizhao Wu
Summary: ST-4 activates specific types of T cells to produce high levels of granzyme B and perforin, which exhibit anticancer effects. Inhibition of granzyme B and perforin weakens the anticancer effect of ST-4, indicating its potential in cancer immunotherapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Immunology
Vinicius O. Boldrini, Ana M. Marques, Raphael P. S. Quintiliano, Adriel S. Moraes, Carla R. A. V. Stella, Ana Leda F. Longhini, Irene Santos, Marilia Andrade, Breno Ferrari, Alfredo Damasceno, Rafael P. D. Carneiro, Carlos Otavio Brandao, Alessandro S. Farias, Leonilda M. B. Santos
Summary: This study found that CD19(+) B cells in the peripheral blood of multiple sclerosis (MS) patients may express serine-protease granzyme-B (GzmB), resembling cytotoxic CD8(+) T lymphocytes. This finding may provide important clues for investigating the pathogenesis of MS and for the development of new therapeutic interventions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Joanna S. Kritikou, Mariana M. S. Oliveira, Julien Record, Mezida B. Saeed, Saket M. Nigam, Minghui He, Marton Keszei, Arnika K. Wagner, Hanna Brauner, Anton Sendel, Saikiran K. Sedimbi, Stamatina Rentouli, David P. Lane, Scott B. Snapper, Klas Karre, Peter Vandenberghe, Jordan S. Orange, Lisa S. Westerberg
Summary: X-linked neutropenia (XLN) is caused by gain-of-function mutations in the actin regulator Wiskott-Aldrich Syndrome protein (WASp), leading to reduced numbers of cytotoxic cells in patients. However, NK and T cells from XLN patients show increased granzyme B content and enhanced degranulation and cytokine production compared to healthy controls, indicating an increased capacity to respond to and kill tumor cells.
Article
Biology
Violeta Soljic, Maja Barbaric, Martina Vukoja, Marina Curlin, Martina Orlovic Vlaho, Edita Cerni Obrdalj, Lidija Lasic Arapovic, Daniela Bevanda Glibo, Katarina Vukojevic
Summary: The study indicates that the expression of cytotoxic CD8(+) T cells in decidual tissue of patients with preeclampsia is altered, suggesting their potential role in the pathophysiology of preeclampsia and fetal-maternal immune tolerance.
Article
Multidisciplinary Sciences
Abhinav Kaushik, Diane Dunham, Xiaorui Han, Evan Do, Sandra Andorf, Sheena Gupta, Andrea Fernandes, Laurie Elizabeth Kost, Sayantani B. Sindher, Wong Yu, Mindy Tsai, Robert Tibshirani, Scott D. Boyd, Manisha Desai, Holden T. Maecker, Stephen J. Galli, R. Sharon Chinthrajah, Rosemarie H. DeKruyff, Monali Manohar, Kari C. Nadeau
Summary: This study analyzed immune cells from participants in a peanut oral immunotherapy (OIT) trial and found that the differentiation status of CD8(+) T cells at baseline may predict the likelihood of achieving sustained unresponsiveness (SU). The study also found that lower frequencies of naïve CD8(+) T cells and terminally differentiated CD57(+)CD8(+) T cell subsets at baseline were associated with SU, while higher frequencies of IL-4(+) and IFN gamma(+) CD4(+) T cells post-OIT were correlated with a lower likelihood of SU.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Iride Mascheretti, Michela Alfieri, Massimiliano Lauria, Franca Locatelli, Roberto Consonni, Erica Cusano, Romeo A. Dougue Kentsop, Marina Laura, Gianluca Ottolina, Franco Faoro, Monica Mattana
Summary: In this study, Linum austriacum in vitro cultures, cells, adventitious roots, and hairy roots were developed for the production of justicidin B through elicitation with MeJA and COR, with the highest productivity observed in the adventitious roots and hairy roots. The synthesis of justicidin B was induced more than three times by MeJA and COR treatments. RNA sequencing and microscopic analysis provided insights into the pathway and cellular localization of justicidin B in L. austriacum.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Neha Tripathi, Richard Danger, Melanie Chesneau, Sophie Brouard, Adele D. Laurent
Summary: This study used microsecond classical molecular dynamics simulations to explore the structural dynamics of active and inactive hGzmB in the catalytic cycle, revealing the involvement of Arg216 in the interactions within the catalytic triad.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2022)
Article
Biochemistry & Molecular Biology
Rongqing Pan, Jeremy Ryan, Deng Pan, Kai W. Wucherpfennig, Anthony Letai
Summary: The mitochondrial apoptosis pathway is crucial for efficient NK cell killing, with pre-activated NK cells demonstrating resistance to BH3 mimetics. Combining BH3 mimetics with NK cells synergistically kills cancer cells and inhibits tumor growth in vivo.
Article
Chemistry, Multidisciplinary
Lingling Xu, Nanhui Liu, Wenjun Zhan, Yu Deng, Zhaoxia Chen, Xiaoyang Liu, Ge Gao, Qian Chen, Zhuang Liu, Gaolin Liang
Summary: The study developed a near-infrared fluorescence probe Cy5.5-CBT-NPs for noninvasive and sensitive evaluation of cancer immunotherapy efficacy. The probe enabled real-time monitoring and enhancement of cytotoxic T lymphocytes (CTLs) responses against tumor cells.
Article
Chemistry, Physical
Tao Huang, Xiaofan Sun, Yingqiu Qi, Xi Yang, Linyao Fan, Mengdie Chen, Yale Yue, Hong Ge, Yiye Li, Guangjun Nie, Huan Min, Xianfu Sun
Summary: This study develops a targeted nanomedicine for breast cancer chemo-immunotherapy, which can induce immunogenic cell death (ICD) to kill cancer cells and stimulate antitumor immune response. The nanomedicine also competitively relieves the inhibition of serpinb9 (Sb9) to granzyme B (GrB), enhancing the combined antitumor effect.
Article
Oncology
Julia Femel, Luuk van Hooren, Melanie Herre, Jessica Cedervall, Falk Saupe, Elisabeth J. M. Huijbers, Danielle R. J. Verboogen, Matthias Reichel, Victor L. Thijssen, Arjan W. Griffioen, Lars Hellman, Anna Dimberg, Anna-Karin Olsson
Summary: Galectin-1 (Gal1) is a glycan-binding protein that promotes tumor progression through various mechanisms. Vaccination against Gal1 can generate high anti-Gal1 antibody levels and significantly impairs the growth of Gal1-expressing melanomas in mice. This is associated with improved tumor vasculature perfusion and increased infiltration of macrophages and cytotoxic T cells. Gal1 vaccination is a promising approach to enhance immunotherapeutic strategies for cancer therapy.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Public, Environmental & Occupational Health
Naoko Kumagai-Takei, Yasumitsu Nishimura, Hidenori Matsuzaki, Suni Lee, Kei Yoshitome, Tatsuo Ito, Takemi Otsuki
Summary: This study found that exposure to asbestos fibers leads to dysfunctional machinery in induced CTLs, which can be partially compensated by IL-15 supplementation. IL-15 is more effective than IL-2 in recovering proliferation and granzyme B levels from asbestos-induced suppression of CTL induction.
ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE
(2021)
Article
Multidisciplinary Sciences
Andreia L. Pinto, Ranjit K. Rai, Jonathan C. Brown, Paul Griffin, James R. Edgar, Anand Shah, Aran Singanayagam, Claire Hogg, Wendy S. Barclay, Clare E. Futter, Thomas Burgoyne
Summary: Ultrastructural studies reveal the mechanisms of SARS-CoV-2 infection within host cells, with ciliated cells being the primary target. The virus enters the cells through fusion with the plasma membrane, releasing the nucleoprotein-encapsidated genome, while viral budding occurs through fusion with membrane compartments.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Naoya Nakagawa, Yutaka Fujisawa, Huihui Xiang, Hidemitsu Kitamura, Keigo Nishida
Summary: Zinc is an essential trace element with various functions in the body. Zinc deficiency can cause immune abnormalities. This study focused on tumor immunity to investigate the effect of zinc on colorectal cancer. The results showed that zinc intake significantly reduced the number of tumors in the colon, and this inhibitory effect was dependent on T cells. Zinc also increased the transcription of granzyme B, an important molecule in tumor immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Yorleny Vicioso, Derek P. Wong, Nand K. Roy, Nayanika Das, Keman Zhang, Parameswaran Ramakrishnan, Reshmi Parameswaran
Summary: Germline deficiency of NF-kappa B c-Rel leads to a marked decrease in cytotoxic function of NK cells, which is regulated by c-Rel through controlling the expression of perforin and granzyme B. The study reveals a crucial role of c-Rel in the cytotoxic function of NK cells through transcriptional regulation of perforin and granzyme B expressions.
FRONTIERS IN IMMUNOLOGY
(2021)