Article
Oncology
Iskander Madhi, Ji-Hee Kim, Ji Eun Shin, Younghee Kim
Summary: Ginsenoside Re (G-Re) inhibits the production of inflammatory mediators in microglial cells and protects hippocampal cells from inflammatory and neurotoxic factors. This suggests G-Re may be a potential treatment option for neuroinflammatory disorders and various neurodegenerative diseases.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Pharmacology & Pharmacy
Gang Zhong, Huiping Long, Fei Chen, Yin Yu
Summary: In this study, oxoglaucine was found to attenuate progression of deterioration and pathological changes in osteoarthritis by activating autophagy and inhibiting calcium influx. It suppressed expression of proinflammatory and apoptosis-related proteins, prevented matrix degradation in OA chondrocytes, and blocked Ca2+ influx while up-regulating autophagic markers. Oxoglaucine shows potential as a candidate drug for OA treatment.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Anastasiia Bohush, Wieslawa Lesniak, Serge Weis, Anna Filipek
Summary: Parkinson's disease is a neurodegenerative disorder characterized by the formation of Lewy bodies and loss of dopaminergic neurons. Changes in intracellular Ca2+ levels can have serious consequences on cells, with calmodulin playing a crucial role in regulating neuronal plasticity. Therefore, alterations in the activity of calmodulin and its binding proteins may be linked to the development and progression of PD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Yuna Amemiya, Masatoshi Maki, Hideki Shibata, Terunao Takahara
Summary: Environmental factors play a crucial role in regulating cell growth and proliferation. The mTOR pathway is a central regulator that maintains cellular homeostasis in response to various stimuli. Dysregulation of mTOR signaling is implicated in diseases such as diabetes and cancer. While the involvement of calcium ion in mTOR signaling has been reported, the molecular mechanisms underlying this regulation are not fully understood. Understanding the role of Ca2+-regulated mTOR signaling, particularly through calcium-binding proteins like calmodulin, is of utmost importance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
Ji Cao, Jingjing Zhang, Jianan Qian, Xue Wang, Wei Zhang, Xiangfan Chen
Summary: This study investigated the effect of RIPK3-regulated CaMKII on necroptosis in heart failure and its mechanism. The results showed that depletion of RIPK3 alleviated cardiac dysfunction, CaMKII activation, and necroptosis. These findings provide valuable insights into the pathogenesis and treatment strategy of heart failure.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Cell Biology
Samar Sayedyahossein, Louise Thines, David B. Sacks
Summary: The Hippo signaling pathway regulates various cellular processes, including cell proliferation, differentiation, migration, and mechanosensation. Calcium ion (Ca2+) has been found to influence core components of the Hippo pathway. However, the crosstalk between Ca2+ signaling and the Hippo pathway is not fully understood.
CELLULAR SIGNALLING
(2023)
Article
Multidisciplinary Sciences
Premchand Rajeev, Nivedita Singh, Adel Kechkar, Cory Butler, Narendrakumar Ramanan, Jean-Baptiste Sibarita, Mini Jose, Deepak Nair
Summary: Synapse associated protein-97/Human Disk Large (SAP97/hDLG) is a conserved, alternatively spliced, modular, scaffolding protein critical in regulating the molecular organization of cell-cell junctions in vertebrates. The molecular determinants of first order phase transition of SAP97/hDLG is controlled by morpho-functional changes in its nanoscale organization. Additionally, the nanoscale molecular signatures of these signaling islands and phase transitions are altered in response to changes in cytosolic Ca2+, showing differential sensitivities to Ca2+ bound Calmodulin. SAP97/hDLG forms nanosized molecular domains in both heterologous cells and hippocampal pyramidal neurons, with molecular signatures and exchange kinetics being modulated by the availability of alternatively spliced isoforms.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Dawei Yang, Rong Rong, Rongliang Yang, Mengling You, Mengxiao Wang, Haibo Li, Dan Ji
Summary: This study elucidated the molecular mechanism of retinal cell damage caused by blue light, revealing that the CaMKII-Drp1 pathway plays a significant role in mediating this damage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biology
Eider Nunez, Frederick Jones, Arantza Muguruza-Montero, Janire Urrutia, Alejandra Aguado, Covadonga Malo, Ganeko Bernardo-Seisdedos, Carmen Domene, Oscar Millet, Nikita Gamper, Alvaro Villarroel, Henry M. Colecraft
Summary: Neuronal K(V)7 channels are highly sensitive to reactive oxygen species, and the S2S3 linker of the voltage sensor plays a crucial role in redox modulation of the channels. Recent structural studies have revealed interactions between this linker and the Ca2+-binding loop of calmodulin, specifically the EF3 hand, which is essential for the signaling. Disrupting Ca2+ binding to EF3 abolishes the oxidation-induced enhancement of K(V)7.4 currents. Furthermore, the loading of EF3 with Ca2+ is critical for the reorientation of the AB fork, which translates the Ca2+ signals.
Article
Microbiology
Guoqi Li, Shaoting Liu, Lijuan Wu, Xiao Wang, Rongrong Cuan, Yongliang Zheng, Deli Liu, Yongze Yuan
Summary: In this study, a new CaMK gene PiCaMK1 from the phytopathogenic fungus P. italicum was cloned and functionally characterized. Knockout of PiCaMK1 resulted in reduced vegetative growth, conidiation, and virulence, as well as decreased sensitivity to salinity and osmotic stress. Transcriptome analysis revealed the involvement of PiCaMK1 in regulating various physical and cellular processes.
Article
Biology
Jeannette Delva-Wiley, Ese S. Ekhator, Laquaundra L. Adams, Supriya Patwardhan, Ming Dong, Robert H. Newman
Summary: The effects of oxidation on the activity of PKA-C α towards different substrates were investigated in this study. The results showed that oxidation differentially affects the activity of PKA-C α towards different substrates. This study provides novel insights into the crosstalk between redox- and phosphorylation-dependent signaling pathways mediated by PKA and lays the foundation for future studies on the role of redox-dependent modification of kinase substrate selection in physiological and pathological states.
Review
Plant Sciences
Julia Medrano-Macias, Adriana Carolina Flores-Gallegos, Erika Nava-Reyna, Isidro Morales, Gonzalo Tortella, Susana Solis-Gaona, Adalberto Benavides-Mendoza
Summary: This review highlights the metabolism and functions of reactive oxygen, nitrogen, and sulfur species (RONSS) in plants' stress acclimation and adaptation. RONSS not only serve as an energy dissipation mechanism but also play essential roles in signaling processes by modifying gene expression and biochemical and physiological adjustments.
Review
Biochemistry & Molecular Biology
Hiroshi Tokumitsu, Hiroyuki Sakagami
Summary: CaMKK acts as the activating kinase for multiple downstream kinases, playing important roles in various Ca2+-dependent pathways as well as pathophysiological pathways. Its molecular mechanism and pharmacological inhibitors are areas of focus in research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Jinshui Yang, Wenli Li, Chao Xing, Guanlan Xing, Yinxue Guo, Hongli Yuan
Summary: Microalgae, as the largest CO2 fixer and O2 producer, play an important role in human life and production. Heat stress has a significant impact on the growth and metabolism of microalgae. This study revealed that Ca2+ is involved in the regulation of lipid synthesis in microalgae under heat stress.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Shoma Araki, Tsuyoshi Takata, Yukihiro Tsuchiya, Yasuo Watanabe
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
E. Doka, T. Ida, M. Dagnell, Y. Abiko, N. C. Luong, N. Balog, T. Takata, B. Espinosa, A. Nishimura, Q. Cheng, Y. Funato, H. Miki, J. M. Fukuto, J. R. Prigge, E. E. Schmidt, E. S. J. Arner, Y. Kumagai, T. Akaike, P. Nagy
Article
Biochemistry & Molecular Biology
Tsuyoshi Takata, Shoma Araki, Yukihiro Tsuchiya, Yasuo Watanabe
ANTIOXIDANTS & REDOX SIGNALING
(2020)
Article
Multidisciplinary Sciences
Koji Osuka, Yasuo Watanabe, Nobuteru Usuda, Kenichiro Iwami, Shigeru Miyachi, Masakazu Takayasu
Article
Multidisciplinary Sciences
Shotaro Kamata, Takuji Oyama, Kenta Saito, Akihiro Honda, Yume Yamamoto, Keisuke Suda, Ryo Ishikawa, Toshimasa Itoh, Yasuo Watanabe, Takahiro Shibata, Koji Uchida, Makoto Suematsu, Isao Ishii
Review
Biochemistry & Molecular Biology
Tsuyoshi Takata, Shoma Araki, Yukihiro Tsuchiya, Yasuo Watanabe
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Critical Care Medicine
Koji Osuka, Yusuke Ohmichi, Mika Ohmichi, Takahiro Nakura, Kenichiro Iwami, Yasuo Watanabe, Shigeru Miyachi
Summary: In the surgical treatment of chronic subdural hematoma, concentrations of all chemokines decreased after irrigation, but IL-8, GRO-alpha, ENA-78, MCP-1, and MPO significantly increased on day 1. These chemokines may play important roles in the early healing process of chronic subdural hematoma.
JOURNAL OF NEUROTRAUMA
(2021)
Article
Immunology
Koji Osuka, Yasuo Watanabe, Chiharu Suzuki, Kenichiro Iwami, Shigeru Miyachi
Summary: Neutrophils play a role in inducing inflammation after subarachnoid hemorrhage (SAH) by migrating into the cerebrospinal fluid (CSF) with the help of chemotactic factors sequentially.
JOURNAL OF NEUROIMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tomohiro Sawa, Tsuyoshi Takata, Tetsuro Matsunaga, Hideshi Ihara, Hozumi Motohashi, Takaaki Akaike
Summary: Polysulfide species, highly nucleophilic in nature, play a crucial role in antioxidant activities. Recent studies have shown that these species undergo hydrolysis to form nucleophilic hydropersulfide/polysulfide and electrophilic sulfenic acid species. Understanding the metabolism and occurrence of polysulfide species is critical for further exploration in this field.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Article
Biochemistry & Molecular Biology
Tsuyoshi Takata, Minkyung Jung, Tetsuro Matsunaga, Tomoaki Ida, Masanobu Morita, Hozumi Motohashi, Xinggui Shen, Christopher G. Kevil, Jon M. Fukuto, Takaaki Akaike
Summary: The article discusses the endogenous production of sulfides and persulfides in mammalian and human cells, highlighting the importance of these metabolites in cellular redox processes. Persulfides are shown to be active antioxidants and redox signaling molecules, with potential roles in energy metabolism. The study also emphasizes the need for advanced techniques to detect and quantify sulfides and persulfides in order to fully understand their functions in cells and tissues.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Qamarul Hafiz Zainol Abidin, Tomoaki Ida, Masanobu Morita, Tetsuro Matsunaga, Akira Nishimura, Minkyung Jung, Naim Hassan, Tsuyoshi Takata, Isao Ishii, Warren Kruger, Rui Wang, Hozumi Motohashi, Masato Tsutsui, Takaaki Akaike
Summary: Reactive sulfur species, such as cysteine hydropersulfide and glutathione persulfide, are produced abundantly in both prokaryotes and eukaryotes, including mammals. Cysteinyl-tRNA synthetase (CARS) has been identified as a new cysteine persulfide synthase (CPERS) responsible for the production of most reactive persulfides. However, the contribution of other enzymes, such as 3-mercaptopyruvate sulfurtransferase (3-MST), cystathionine beta-synthase (CBS), and cystathionine gamma-lyase (CSE), to reactive persulfide production is still debated. In this study, the authors used sulfur metabolome analysis to demonstrate that 3-MST, CBS, and CSE are not major sources of reactive persulfides in mammals, and CARS/CPERS is the principal enzyme involved.
Review
Biochemistry & Molecular Biology
Shoma Araki, Koji Osuka, Tsuyoshi Takata, Yukihiro Tsuchiya, Yasuo Watanabe
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Julia Claudiane da Veiga, Neidiquele Maria Silveira, Amedea Barozzi Seabra, Ilana Urbano Bron
Summary: This review investigates the strategy of using nitric oxide (NO) donors to extend postharvest shelf-life and improve fruit quality, discusses the interactions between NO and ethylene or reactive oxygen species, and highlights the potential of nanotechnology in NO applications.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2024)