Journal
MOLECULES
Volume 24, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/molecules24101863
Keywords
G-quadruplex; central loop length; thermal stability; entropy-driven; ionic effect
Funding
- National Natural Science Foundation of China [81371896]
- Natural Science Foundation of Liaoning Province [2013225089]
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The central loop of G-quadruplex molecular beacons is a key element to sense target DNA or RNA sequences. In this study, circular dichroism spectroscopy (CD), thermal difference spectrum (TDS), non-denatured non-denaturing gel electrophoresis, and thermal stability analysis were used to investigate the effect of the central loop length on G-quadruplex features. Two series of G-quadruplexes, AG(3)TTAG(3)-(TTA)n-G(3)TTAG(3)T (n = 1-8) (named TTA series) and AG(3)TTTG(3)-(TTA)n-G(3)TTTG(3)T (n = 1-8) (named TTT series) were examined in K+ and Na+ solutions, respectively. CD and TDS spectral data indicated that TTA series adopted an antiparallel G-quadruplex structure in Na+ solution and a hybrid G-quadruplex structure in K+ solution respectively. TTT series exhibited a hybrid G-quadruplex structure in both Na+ and K+ solutions. UV melting curves indicated that the stability of G-quadruplex in both series was reduced by the elongation of central loop. Thermal stability analysis concluded that the G-quadruplex destabilization with long central loop is an entropy-driven process due to more flexible and longer central loops.
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