Article
Microbiology
Lauren M. Hawkins, Anatoli V. Naumov, Mrinalini Batra, Changqi Wang, Dale Chaput, Elena S. Suvorova
Summary: This study investigates the process of endodyogeny in Toxoplasma gondii and discovers a novel parasite-specific complex, TgCrk6/TgCyc1, that regulates the spindle assembly checkpoint. The study demonstrates the involvement of parasite-specific tasks in the canonical checkpoint functions and provides insights into the unique replication mechanisms of Apicomplexa parasites.
Article
Microbiology
Lauren M. Hawkins, Anatoli Naumov, Mrinalini Batra, Changqi Wang, Dale Chaput, Elena S. Suvorova
Summary: In this study, a novel parasite-specific complex, TgCrk6/TgCyc1, was discovered to play a role in regulating the spindle assembly checkpoint. The evolution of replication mechanisms in Toxoplasma differs significantly from conventional cell cycles, with the incorporation of parasite-specific tasks providing essential tools to control the acute stage of infection.
Article
Biology
Taylor P. Enrico, Wayne Stallaert, Elizaveta T. Wick, Peter Ngoi, Xianxi Wang, Seth M. Rubin, Nicholas G. Brown, Jeremy E. Purvis, Michael J. Emanuele
Summary: Cell cycle gene expression programs are dysregulated in cancer, and the RB-family of proteins play a key role in repressing cell cycle gene expression and inhibiting proliferation. Phosphorylation and ubiquitination are crucial in cell cycle control, and the SCFcyclin F pathway may regulate the RB-network by affecting p130 degradation, potentially leading to cell cycle dysregulation in human cancers.
Article
Cell Biology
Savitha S. Sharma, W. Jack Pledger, Paturu Kondaiah
Summary: Cyclin F interacts with the substrate recognition subunit and deubiquitylase USP7 to play a critical role in the regulation of centrosomal duplication, DNA replication and repair, and maintenance of genomic stability.
Article
Biochemistry & Molecular Biology
Aroa Rey Campa, Leah M. Smith, Hannah G. Hampton, Sahil Sharma, Simon A. Jackson, Thorsten Bischler, Cynthia M. Sharma, Peter C. Fineran
Summary: Studies have shown that the Rsm/Csr pathway plays a key role in regulating the expression of multiple CRISPR-Cas systems in Serratia, allowing modulation of adaptive immunity through coregulation of CRISPR-Cas and flagella.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Shan Jiang, Jinwei Wei, Na Li, Zhibiao Wang, Yilan Zhang, Ran Xu, Lixun Zhou, Xiahe Huang, Li Wang, Siyi Guo, Yingchun Wang, Chun-Peng Song, Wei Qian, Yunhai Li
Summary: This study identifies a regulatory cascade involving UBP14/DA3-CDKB1;1-CDKG2/SUD6 that plays a crucial role in controlling endoreduplication and cell growth in Arabidopsis.
Article
Biochemistry & Molecular Biology
Nan Wang, Yao Deng, Lisha Zhang, Yingchun Wan, Ting Lei, Yimin Yang, Can Wu, Hai Du, Ping Feng, Wuzhong Yin, Guanghua He
Summary: A rice mutant, osuge1, lacking UDP-glucose epimerase activity, exhibited delayed tapetum degradation and abnormal pollen development. OsUGE1 was found to participate in the TIP2/bHLH142-TDR-EAT1/DTD transcriptional regulatory cascade, in which TIP2 and TDR regulate OsUGE1 expression while OsUGE1 regulates EAT1 expression. Moreover, OsUGE1 was shown to regulate its own gene expression by directly binding to an E-box element in its promoter. These findings demonstrate that OsUGE1 has moonlighting functions as a transcriptional activator in addition to its role as a UDP-glucose epimerase.
Article
Chemistry, Medicinal
Jiacheng Li, Ting Liu, Yuanli Song, Mingyu Wang, Liping Liu, Hongwen Zhu, Qi Li, Jin Lin, Hualiang Jiang, Kaixian Chen, Kehao Zhao, Mingliang Wang, Hu Zhou, Hua Lin, Cheng Luo
Summary: In this study, the hydrophobic tagging method was applied for the first time to induce degradation of the CDK9-cyclin T1 heterodimer, which plays a critical role in cancer cells. LL-K9-3 was identified as a potent small-molecule degrader of CDK9-cyclin T1, selectively degrading both proteins and reducing the expression of AR and cMyc. Compared with existing CDK9 inhibitors, LL-K9-3 exhibited enhanced anti-proliferative and pro-apoptotic effects, as well as more effective suppression of downstream signaling of CDK9 and AR. Additionally, LL-K9-3 inhibited oncogenic transcriptional programs driven by AR and Myc, and had stronger inhibitory effects on AR's intrinsic target genes.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Magdalena Modrzejewska, Adam Kawalek, Aneta Agnieszka Bartosik
Summary: This study reveals that BsrA, a LysR-type transcriptional regulator from Pseudomonas aeruginosa, acts as a part of an intricate global regulatory network, directly or indirectly repressing and/or activating genes from essential metabolic pathways and controlling transport functions and surface appendage formation. Expression of the bsrA gene is induced in the presence of antibiotics, indicating its role in redirecting metabolism under stressful conditions. These findings highlight the important functions of the BsrA regulator in carbon metabolism, biofilm formation, and antibiotic resistance in P. aeruginosa.
Article
Microbiology
Jun Zhang, Yi-Fei Wu, Shi-Tong Tang, Jian Chen, Barry P. Rosen, Fang-Jie Zhao
Summary: In this study, it was discovered that PadR(ars) can directly bind to the promoter region of the arsenic resistance operon and repress the transcription of arsV and arsW genes involved in MAs(III) resistance. The repressor protein PadR(ars) binds to two inverted-repeat sequences in the ars promoter to inhibit transcription. Furthermore, it was found that Cys56/57 is responsible for binding trivalent metalloids while C133 or C134 acts as a third ligand to trivalent metalloids.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Elia Mascolo, Satish Adhikari, Steven M. Caruso, Tagide deCarvalho, Anna Folch Salvador, Joan Serra-Sagrista, Ry Young, Ivan Erill, Patrick D. Curtis
Summary: Pilitropic and flagellotropic phages use bacterial pili and flagella for adsorption. Research has shown that these phages can synchronize their lysis with the host cell cycle, providing an adaptive advantage for infecting bacterial cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yi Chen, Zhiwei Xu, Yanli Zeng, Junping Liu, Xuemei Wang, Yi Kang
Summary: Autophagy plays a crucial role in liver regeneration by promoting hepatocellular proliferation through maintaining normal hepatic and systemic metabolism, while suppressing hepatocellular apoptosis.
Article
Biochemistry & Molecular Biology
Christine A. Mills, Xianxi Wang, Dhaval P. Bhatt, Paul A. Grimsrud, Jacob Peter Matson, Debojyoti Lahiri, Daniel J. Burke, Jeanette Gowen Cook, Matthew D. Hirschey, Michael J. Emanuele
Summary: This study identified SIRT5 as a novel substrate of Cyclin F, which regulates cell cycle and metabolism through interaction with Cyclin F. The knockout of SIRT5 results in changes in cell cycle signaling congruent with S and G2/M phase increase. These findings suggest a connection between SIRT5, cell cycle regulation, and metabolism.
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Article
Plant Sciences
Paul Bolay, Susan Schluter, Samuel Grimm, Matthias Riediger, Wolfgang R. Hess, Stephan Klaehn
Summary: Cyanobacteria evolved a CO2 concentrating mechanism to mitigate the ineffective fixation of CO2 by RuBisCO, however, the regulation of these carbon assimilatory systems is still not fully understood. By studying a model cyanobacterium, an essential transcriptional regulator Sll0998 was identified, which controls the activity of RuBisCO, crucial for engineering cyanobacterial cell factories.
Article
Biochemistry & Molecular Biology
Luojiang Huang, Kai Hua, Ran Xu, Dali Zeng, Ruci Wang, Guojun Dong, Guozheng Zhang, Xueli Lu, Na Fang, Dekai Wang, Penggen Duan, Baolan Zhang, Zupei Liu, Na Li, Yuehua Luo, Qian Qian, Shanguo Yao, Yunhai Li
Summary: The study reveals that the gene LARGE2 encodes the protein OsUPL2 in rice, which regulates panicle size and grain number by repressing meristematic activity. The protein physically interacts with positive regulators APO1 and APO2 to modulate their stabilities, functioning in a common pathway to regulate panicle size and grain number. These findings provide insights into the genetic and molecular mechanisms controlling panicle size and grain number in rice.
Article
Biochemistry & Molecular Biology
Hui Wang, Hui Shi, Malini Rajan, Elizabeth R. Canarie, Seoyeon Hong, Daniele Simoneschi, Michele Pagano, Matthew F. Bush, Stefan Stoll, Elizabeth A. Leibold, Ning Zheng
Article
Biochemistry & Molecular Biology
Jessica Sook Yuin Ho, Matthew Angel, Yixuan Ma, Elizabeth Sloan, Guojun Wang, Carles Martinez-Romero, Marta Alenquer, Vladimir Roudko, Liliane Chung, Simin Zheng, Max Chang, Yesai Fstkchyan, Sara Clohisey, Adam M. Dinan, James Gibbs, Robert Gifford, Rong Shen, Quan Gu, Nerea Irigoyen, Laura Campisi, Cheng Huang, Nan Zhao, Joshua D. Jones, Ingeborg van Knippenberg, Zeyu Zhu, Natasha Moshkina, Lea Meyer, Justine Noel, Zuleyma Peralta, Veronica Rezelj, Robyn Kaake, Brad Rosenberg, Bo Wang, Jiajie Wei, Slobodan Paessler, Helen M. Wise, Jeffrey Johnson, Alessandro Vannini, Maria Joao Amorim, J. Kenneth Baillie, Emily R. Miraldi, Christopher Benner, Ian Brierley, Paul Digard, Marta Luksza, Andrew E. Firth, Nevan Krogan, Benjamin D. Greenbaum, Megan K. MacLeod, Harm van Bakel, Adolfo Garcia-Sastre, Jonathan W. Yewdell, Edward Hutchinson, Ivan Marazzi
Article
Biochemistry & Molecular Biology
Zharko Daniloski, Tristan X. Jordan, Hans-Hermann Wessels, Daisy A. Hoagland, Silva Kasela, Mateusz Legut, Silas Maniatis, Eleni P. Mimitou, Lu Lu, Evan Geller, Oded Danziger, Brad R. Rosenberg, Hemali Phatnani, Peter Smibert, Tuuli Lappalainen, Benjamin R. tenOever, Neville E. Sanjana
Summary: Researchers conducted a genome-scale CRISPR screen to identify host factors crucial for SARS-CoV-2 infection, highlighting genes related to ATPase proton pump, Retromer, and Commander complexes. Validating these gene targets through multiple methods, they found shared transcriptional changes in cholesterol biosynthesis when top-ranked genes were lost. Loss of RAB7A was shown to reduce viral entry by sequestering the ACE2 receptor inside cells, underscoring its importance early in the viral infection process.
Article
Biology
Hajnalka L. Palinkas, Angela Bekesi, Gergely Rona, Lorinc Pongor, Gabor Papp, Gergely Tihanyi, Eszter Holub, Adam Poti, Carolina Gemma, Simak Ali, Michael J. Morten, Eli Rothenberg, Michele Pagano, David Szuts, Balazs Gyorffy, Beata G. Vertessy
Article
Biology
Roosheel S. Patel, Joy E. Tomlinson, Thomas J. Divers, Gerlinde R. Van de Walle, Brad R. Rosenberg
Summary: This study demonstrates the utility of single-cell RNA sequencing (scRNA-Seq) in characterizing cellular diversity of equine peripheral blood mononuclear cells (PBMC), identifying a majority of T-bet(+) B cells in the equine peripheral B cell compartment, which is typically associated with chronic infection and inflammation in human and mouse.
Editorial Material
Cell Biology
Luca Lignitto, Michele Pagano
Summary: Cell-cell fusion, crucial for the development of multicellular organisms, is driven by the remodeling of the actin cytoskeleton. A recent study reveals how CRL3-dependent mono-ubiquitylation regulates cell fusion by controlling the dynamics of cytoskeletal rearrangements.
DEVELOPMENTAL CELL
(2021)
Article
Gastroenterology & Hepatology
Joy E. Tomlinson, Raphael Wolfisberg, Ulrik Fahnoe, Roosheel S. Patel, Sheetal Trivedi, Arvind Kumar, Himanshu Sharma, Louise Nielsen, Sean P. McDonough, Jens Bukh, Bud C. Tennant, Amit Kapoor, Brad R. Rosenberg, Charles M. Rice, Thomas J. Divers, Gerlinde R. Van de Walle, Troels K. H. Scheel
Summary: EqHV infection in horses typically has an acute resolving course, and the protective immune response lasts for at least a year, which could have important implications for the development of an HCV vaccine.
Article
Biochemistry & Molecular Biology
Marco Salamina, Bailey C. Montefiore, Mengxi Liu, Daniel J. Wood, Richard Heath, James R. Ault, Lan-Zhen Wang, Svitlana Korolchuk, Arnaud Basle, Martyna W. Pastok, Judith Reeks, Natalie J. Tatum, Frank Sobott, Stefan T. Arold, Michele Pagano, Martin E. M. Noble, Jane A. Endicott
Summary: The SCFSKP2 ubiquitin ligase promotes degradation of p27KIP1 to relieve G1 checkpoint control of CDK-cyclin complexes, ensuring orderly cell cycle progression. The interaction between SKP2 and cyclin A provides a structural mechanism for fine-tuning the degradation of p27KIP1 and distinguishes cyclin A from other G1 cyclins.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Emiliano Maiani, Giacomo Milletti, Francesca Nazio, Sos Gronbaek Holdgaard, Jirina Bartkova, Salvatore Rizza, Valentina Cianfanelli, Mar Lorente, Daniele Simoneschi, Miriam Di Marco, Pasquale D'Acunzo, Luca Di Leo, Rikke Rasmussen, Costanza Montagna, Marilena Raciti, Cristiano De Stefanis, Estibaliz Gabicagogeascoa, Gergely Rona, Nelida Salvador, Emanuela Pupo, Joanna Maria Merchut-Maya, Colin J. Daniel, Marianna Carinci, Valeriana Cesarini, Alfie O'sullivan, Yeon-Tae Jeong, Matteo Bordi, Francesco Russo, Silvia Campello, Angela Gallo, Giuseppe Filomeni, Letizia Lanzetti, Rosalie C. Sears, Petra Hamerlik, Armando Bartolazzi, Robert E. Hynds, David R. Pearce, Charles Swanton, Michele Pagano, Guillermo Velasco, Elena Papaleo, Daniela De Zio, Apolinar Maya-Mendoza, Franco Locatelli, Jiri Bartek, Francesco Cecconi
Summary: AMBRA1 has been identified as a key regulator in cell cycle control, playing a crucial role in maintaining genomic integrity by regulating the abundance of D-type cyclins and facilitating the transition from G1 to S phase, thereby preventing replication stress. The findings suggest that CHK1 kinase could be a potential therapeutic target in AMBRA1-deficient tumors, highlighting the importance of the AMBRA1-cyclin D pathway in regulating cell cycle and genomic stability in embryonic development and tumorigenesis.
Article
Multidisciplinary Sciences
Daniele Simoneschi, Gergely Rona, Nan Zhou, Yeon-Tae Jeong, Shaowen Jiang, Giacomo Milletti, Arnaldo A. Arbini, Alfie O'Sullivan, Andrew A. Wang, Sorasicha Nithikasem, Sarah Keegan, Yik Siu, Valentina Cianfanelli, Emiliano Maiani, Francesca Nazio, Francesco Cecconi, Francesco Boccalatte, David Fenyo, Drew R. Jones, Luca Busino, Michele Pagano
Summary: The research identifies AMBRA1 as a ubiquitin ligase that degrades D-type cyclins, whose loss leads to accumulation of D-type cyclins and tumorigenesis. Furthermore, AMBRA1 also affects the sensitivity to CDK4/6 inhibitors.
Article
Multidisciplinary Sciences
Elijah L. Mena, Callie J. Donahue, Laura Pontano Vaites, Jie Li, Gergely Rona, Colin O'Leary, Luca Lignitto, Bearach Miwatani-Minter, Joao A. Paulo, Avantika Dhabaria, Beatrix Ueberheide, Steven P. Gygi, Michele Pagano, J. Wade Harper, Robert A. Davey, Stephen J. Elledge
Summary: The study found an interaction between ORF10 and CRL2ZYG11B but no evidence that ORF10 is functioning to inhibit or hijack CRL2ZYG11B.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Tony Sun, Yingpu Yu, Xianfang Wu, Ashley Acevedo, Ji-Dung Luo, Jiayi Wang, William M. Schneider, Brian Hurwitz, Brad R. Rosenberg, Hachung Chung, Charles M. Rice
Summary: Defective ADAR1 editing can lead to disorders, with the two protein isoforms p150 and p110 showing different contributions to RNA editing. The challenges in expressing p150 without p110 may explain the differences in editing landscape between the two isoforms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Virology
Benjamin E. Nilsson-Payant, Skyler Uhl, Adrien Grimont, Ashley S. Doane, Phillip Cohen, Roosheel S. Patel, Christina A. Higgins, Joshua A. Acklin, Yaron Bram, Vasuretha Chandar, Daniel Blanco-Melo, Maryline Panis, Jean K. Lim, Olivier Elemento, Robert E. Schwartz, Brad R. Rosenberg, Rohit Chandwani, Benjamin R. TenOever
Summary: Research suggests that in SARS-CoV-2 infection, NF-kappa B activation plays a crucial role in viral replication in the absence of traditional IFN-I-related transcription factors. Silencing NF-kappa B transcription factors or using inhibitors can reduce virus replication. These findings contribute to a better understanding of SARS-CoV-2 replication mechanisms and provide a basis for the development of novel treatment approaches.
JOURNAL OF VIROLOGY
(2021)
Article
Oncology
Jiehui Deng, Aatish Thennavan, Igor Dolgalev, Ting Chen, Jie Li, Antonio Marzio, John T. Poirier, David H. Peng, Mirna Bulatovic, Subhadip Mukhopadhyay, Heather Silver, Eleni Papadopoulos, Val Pyon, Cassandra Thakurdin, Han Han, Fei Li, Shuai Li, Hailin Ding, Hai Hu, Yuanwang Pan, Vajira Weerasekara, Baishan Jiang, Eric S. Wang, Ian Ahearn, Mark Philips, Thales Papagiannakopoulos, Aristotelis Tsirigos, Eli Rothenberg, Justin Gainor, Gordon J. Freeman, Charles M. Rudin, Nathanael S. Gray, Peter S. Hammerman, Michele Pagano, John V. Heymach, Charles M. Perou, Nabeel Bardeesy, Kwok-Kin Wong
Summary: The study found that LKB1-deficient lung tumors are sensitive to autophagy inhibition, which can restore impaired antigen presentation and antitumor immune responses, enhancing the effectiveness of PD-1 immunotherapy. Additionally, LKB1 deficiency inhibits antigen processing and presentation, but this can be reversed by targeting the autophagy pathway to restore immunoproteasome activity and antigen presentation.
Article
Oncology
Loredana Moro, Michele Pagano
MOLECULAR & CELLULAR ONCOLOGY
(2020)