Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Anita Tolpinrud, John Stenos, Anne-Lise Chaber, Joanne M. Devlin, Catherine Herbert, An Pas, Magdalena Dunowska, Mark A. Stevenson, Simon M. Firestone
Summary: In this study, the prevalence of Coxiella burnetii infection in macropod populations was investigated. A phase-specific indirect immunofluorescence assay (IFA) was modified and optimized to estimate the infection rate. The results showed that the IFA had high diagnostic sensitivity and specificity. Meanwhile, the commercially available enzyme-linked immunosorbent assay (ELISA) showed low sensitivity.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Ziyi He, Justin Huffman, Kathrine Curtin, Krista L. Garner, Elizabeth C. Bowdridge, Xiaojun Li, Timothy R. Nurkiewicz, Peng Li
Summary: The cPlate system, enabled by a novel microscale fluid manipulation method, allows for highly scalable multiplexed ELISA analysis with high sensitivity and specificity, shorter assay time, and lower consumption, making it suitable for point-of-care testing in resource-limited regions.
ANALYTICAL CHEMISTRY
(2021)
Article
Immunology
A-Yeung Jang, Min-Joo Choi, Yong Zhi, Hyun-Jung Ji, Ji-Yun Noh, Jin-Gu Yoon, Hee-Jin Cheong, Woo-Joo Kim, Ho-Seong Seo, Joon-Young Song
Summary: The standardized GBS-ELISA developed in this study shows high specificity and accuracy in evaluating the immunogenicity of GBS vaccines against the three main serotypes. The results demonstrate good performance of the ELISA in assessing the three serotypes, aiding in the development and evaluation of GBS vaccines.
Article
Biochemical Research Methods
Filippos Filippatos, Elizabeth -Barbara Tatsi, Christos Papagiannopoulos, Vasiliki Syriopoulou, Athanasios Michos
Summary: The study compared the ELISA and FIC methods for detecting neutralizing antibodies after COVID-19 immunization. The results showed good qualitative agreement between FIC and ELISA in detecting positive neutralizing antibodies, suggesting that FIC could be an alternative rapid testing method.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Medicine, General & Internal
Lotfi S. Bin Dahman, Khalid M. Sumaily, Essa M. Sabi, Mohammed A. Hassan, Abeer M. Bin Thalab, Asrar S. Sayad, Saleh M. Bin Kolaib, Fatima M. Alhadhrmi
Summary: This study compared the serum ferritin levels measured by ELISA, Cobas e411, and Cobas Integra 400 methods. The results showed no significant difference between ELISA and Cobas e411, while the values from Cobas Integra 400 were significantly higher. Cobas e411 and Cobas Integra 400 methods demonstrated higher sensitivity, specificity, and accuracy. Further investigations with larger samples are needed to determine interchangeability.
Article
Biochemical Research Methods
Tetsuya Saita, Hiroto Kataoka, Rintaro Sogawa, Tadashi Hayama, Ryoko Tomita, Akira Monji, Yoshito Mizoguchi, Chisato Shimanoe
Summary: The study successfully developed a simple and specific ELISA method for the quantification of PEA, showing comparable results to HPLC measurements and a strong correlation between the values determined by the two methods.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Chemistry, Analytical
Jian Zhou, Jie Liu, Shanshan Song, Liqiang Liu, Chuanlai Xu, Hua Kuang, Wei Ma
Summary: A sensitive and specific monoclonal antibody was developed to detect Diuron in water and sugarcane samples. An immunochromatographic assay strip based on this antibody showed visual detection limits and critical values for Diuron in water and sugarcane samples, which were validated using HPLC-MS.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Applied
Yuechun Li, Han Zhang, Zhaowen Cui, Sijie Liu, Jingke Xu, Conghui Jia, Yaqian Chen, Lulu Wang, Jing Sun, Daohong Zhang, Mingqiang Zhu, Jianlong Wang
Summary: This research explores a novel strategy to enhance the catalytic activity of enzymes, leading to the development of a sensitive ELISA method. By utilizing a chemical staining strategy, the catalytic activity of horseradish peroxidase was directly improved, and a new CS-ELISA method for detecting clenbuterol was established. The CS-ELISA exhibited a 2-fold improvement in sensitivity and linear range compared to conventional ELISA, and demonstrated good applicability in detecting clenbuterol in pork tenderloin samples.
Article
Chemistry, Analytical
Ying Wang, Tong Bu, Yuanyuan Cao, Haiyu Wu, Jia Xi, Qinlin Feng, Chenyu Xuan, Li Wang
Summary: In this study, a peroxidase-mimicking nanozyme-improved enzyme-linked immunosorbent assay (ELISA) was developed using the bimetallic PdRu nanozyme as a catalytic carrier for detecting Escherichia coli O157:H7. The PdRu nanozyme exhibited high catalytic activity and affinity with antibody, ensuring successful establishment of a novel colorimetric biosensor. The PdRu-based ELISA achieved ultrasensitive detection sensitivity and satisfactory specificity and reproducibility, making it suitable for bioassays and clinical diagnostics.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Meng Wu, Bingjian Zhang, Guoping Sun, Leping Jiang
NEW JOURNAL OF CHEMISTRY
(2017)
Article
Chemistry, Analytical
Wu Meng, Zhang Bingjian, Yang Junchang
MICROCHEMICAL JOURNAL
(2020)
Article
Archaeology
Kuanrong Zhai, Guoping Sun, Yunfei Zheng, Meng Wu, Bingjian Zhang, Longguan Zhu, Qi Hu
Summary: The discovery of the earliest lacquerwares at the Jingtoushan Neolithic site in the Yangtze River Delta confirms that humans in the region were using natural lacquer over 8,000 years ago. This finding also adds to the history of organic coatings used during the Neolithic period in China.
Article
Archaeology
Di Zhang, Meng Wu, Hui Zhu, Bingjian Zhang, Longguan Zhu
Summary: This paper presents a new method for detecting trace amounts of sticky rice in mortar by extracting and purifying protein from sticky rice and using the enzyme linked immunosorbent assay (ELISA). The ELISA detection limit based on sticky rice is significantly lower than that of the traditional iodine-starch method.
Article
Archaeology
Meng Wu, Bingjian Zhang, Qiong Zhang, Qian Cheng, Siwei Jiang, Li Li
Summary: This study prepared polyclonal antibodies to peach gum and used biotin-streptavidin-enzyme-linked immunosorbent assay (BA-ELISA) to identify peach gum with high sensitivity and specificity. Enzyme-linked immunosorbent assay (ELISA) was used to detect binders in the Forbidden City and the Dazu Rock Carvings. The results revealed diverse binders in the Dazu Rock Carvings and the coexistence of collagen and peach gum in most samples of the Forbidden City. This study demonstrates the high efficiency of ELISA in analyzing binders in ancient paintings.
STUDIES IN CONSERVATION
(2023)
Article
Anthropology
Meng Wu, Zhongming Tang, Nan Bi, Qinghua Yang, Hui Fang, Quanyu Wang
Summary: The discovery of a grooming set in an ancient Han tomb in Shandong, China, provides insights into the use of cosmetics by non-elite individuals, highlighting the role of cosmetics in reflecting social cultures. Utilizing various analytical methods, the study identified one cosmetic as a lead hydroxycarbonate powder likely used for whitening, and the other as a flesh-colored mica powder containing refined animal fat and plant extract. The findings suggest that the use of cosmetics had spread from the elite to the non-elite in ancient China, indicating the development of a regional cosmetic industry.
ARCHAEOLOGICAL AND ANTHROPOLOGICAL SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Meng Wu, Yunfan Zhang, Bingjian Zhang, Lan Li
Summary: The Zenghou Yi Tomb from 433 BC is an important archaeological find from the early Warring States Period in China. The tomb contained a significant amount of lacquerware, with red, black, and yellow patterns. Analysis of the lacquered inner coffin and zither indicate that the lacquer samples have multilayered structures and contained added eggs and oil. The differences in pigments used for the red, black, and yellow patterns may explain the rarity of yellow painted lacquerware from early periods.
NEW JOURNAL OF CHEMISTRY
(2021)
