Article
Plant Sciences
Baijin Chang, Yanru Liu, Jingting Hu, Zhishu Tang, Zhidong Qiu, Zhongxing Song, Ailing Jia, Yuru Zhang
Summary: This study investigated the key targets and metabolites of the antidepressant effect of B. chinense based on the cAMP signalling pathway. The results showed that B. chinense significantly improved the depression symptoms in rats, and its antidepressant effect is primarily achieved by regulating the cAMP metabolic pathway.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sandeep Kumar Barodia, Tuithung Sophronea, Pratibha Mehta Luthra
Summary: The study demonstrated that A(2A) receptors modulate IP3 levels to release cytoplasmic calcium via cAMP-dependent PKA, potentially opening new avenues in the therapy of neurodegenerative disorders.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2022)
Article
Oncology
Cong Chen, Shanmin Yuan, Xiaohong Chen, Jiangping Xie, Zheng Wei
Summary: The molecular mechanism of Xihuang pill (XHP) in breast cancer (BC) was investigated using murine mammary carcinoma 4T1 cell xenografts. XHP inhibited BC cell proliferation, migration, and invasion, induced pyroptosis, and activated the cAMP/PKA signalling pathway. Inhibiting the cAMP/PKA signalling pathway reversed the anti-cancer activity of XHP.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Plant Sciences
Kai Yin, Guobing Cui, Xinping Bi, Meiling Liang, Zhijian Hu, Yi Zhen Deng
Summary: This study identified the genes involved in polyamine biosynthesis in the sugarcane smut fungus. These genes play important roles in filamentous growth and pathogenic development of the fungus, possibly by collaborating with the cAMP-PKA signaling pathway to regulate intracellular redox homeostasis. The study provides insights into the regulatory mechanisms of S. scitamineum pathogenesis.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Dennis Botman, Sineka Kanagasabapathi, Philipp Savakis, Bas Teusink
Summary: The AKAR3-EV biosensor measures the activity of PKA and Sch9 kinases in single yeast cells, providing insights into the cellular adaptations of yeast to changing nutrient environments. The biosensor was able to accurately measure the phosphorylation status in intact yeast cells, with heterogeneous responses observed for different sugars. The study highlights the importance of the AKAR3-EV sensor in understanding cellular adaptation in single yeast cells.
FEMS YEAST RESEARCH
(2023)
Article
Cell Biology
Griet Van Zeebroeck, Liesbeth Demuyser, Zhiqiang Zhang, Ines Cottignie, Johan M. Thevelein
Summary: The study investigates the PKA pathway in regulating cell growth in yeast, showing different nutrient activation mechanisms, some mediated by cAMP and others through direct release of catalytic subunits to activate PKA. Nutrient transceptors may be evolutionary ancestors of GPCR, using a more primitive direct signaling mechanism compared to the indirect cAMP signaling of GPCRs.
Article
Biotechnology & Applied Microbiology
Viktor C. Persson, Raquel Perruca Foncillas, Tegan R. Anderes, Clement Ginestet, Marie Gorwa-Grauslund
Summary: Although xylose anomer specificity is often overlooked, the addition of xylose epimerase should be considered as a key engineering step, especially when using the best-performing XI enzyme from L. phytofermentans. Additional research into the binding mechanism of xylose is needed to elucidate the enzyme-specific effect and decrease in xylitol accumulation. Finally, the differences in sugar signaling responses between XI and XR/XDH strains indicate that either the redox balance or the growth rate impacts the SNF1/Mig1p sensing pathway.
BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
(2023)
Review
Biotechnology & Applied Microbiology
Daniel P. Brink, Krithika Ravi, Gunnar Liden, Marie F. Gorwa-Grauslund
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Agricultural Engineering
Krithika Ravi, Javier Garcia-Hidalgo, Daniel P. Brink, Martin Skyvell, Marie F. Gorwa-Grauslund, Gunnar Liden
BIORESOURCE TECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Javier Garcia-Hidalgo, Daniel P. Brink, Krithika Ravi, Catherine J. Paul, Gunnar Liden, Marie F. Gorwa-Grauslund
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Daniel P. Brink, Celina Borgstrom, Viktor C. Persson, Karen Ofuji Osiro, Marie F. Gorwa-Grauslund
Summary: Investigation on the signaling response of Saccharomyces cerevisiae to d-xylose and attempts to improve the response through signaling engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Friederike Mierke, Daniel P. Brink, Joakim Norbeck, Verena Siewers, Thomas Andlid
Summary: In this study, the oleaginous nature of P. hubeiensis was further characterized by evaluating its metabolism and gene expression responses during storage lipid formation conditions with glucose or xylose as a carbon source. The genome of P. hubeiensis BOT-O strain was sequenced, generating the first mRNA-supported genome annotation and identifying 6540 genes. The study revealed that BOT-O can efficiently utilize both glucose and xylose, with glucose being taken up faster during mixed cultivation. Nitrogen-starvation had a significant transcriptional effect on the expression of 1179 genes.
FUNGAL GENETICS AND BIOLOGY
(2023)
Article
Microbiology
Nikhil Seshagiri Rao, Ludwig Ermann Lundberg, Julia Tomasson, Cecilia Tullberg, Daniel P. Brink, Shuai Bai Palmkron, Ed W. J. van Niel, Sebastian Hakansson, Magnus Carlquist
Summary: The study investigated the effects of oxygen on Limosilactobacillus reuteri DSM 17938 during cultivation and the resulting properties of the freeze-dried probiotic product. It was found that low levels of oxygen did not influence the growth kinetics of the bacterium, but did affect the redox metabolism and production of acetate and ethanol. Cells cultivated in the presence of oxygen showed higher survival rates, increased unsaturated fatty acid content, improved bile tolerance, and higher 5' nucleotidase activity. However, they exhibited lower tolerance to acidic conditions compared to bacteria grown under complete anaerobic conditions. Overall, controlled oxygen supply during production can optimize the probiotic activity of L. reuteri DSM 17938.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Daniel P. Brink, Elin M. Larsson, Javier Garcia-Hidalgo, Helga Helgadottir, Marie F. Gorwa-Grauslund
Summary: Two bacterial strains (S1 and S4) capable of using syringol as the only carbon source were isolated from compost and sequenced using the Illumina platform. The final assemblies of S1 and S4 genomes were found to have 4.2 Mbp, 63% GC, and 3,912 genes, and 6.2 Mbp, 64% GC, and 5,503 genes, respectively.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Biotechnology & Applied Microbiology
Venkatachalam Narayanan, Anders G. Sandstrom, Marie F. Gorwa-Grauslund
Summary: Various approaches have been used to improve xylose utilization and ethanol productivity in Saccharomyces cerevisiae, and the BUD21 gene has been identified as a potential candidate. However, the positive effects of BUD21 gene deletion on xylose fermentation were strain- or medium-dependent.
METABOLIC ENGINEERING COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Daniel P. Brink, Friederike Mierke, Joakim Norbeck, Verena Siewers, Thomas Andlid
Summary: In this study, six new R. toruloides promoters that were either upregulated or downregulated under nitrogen-starvation were identified. These promoters are expected to be particularly useful for future engineering strategies requiring specific regulation of target genes in accordance with nitrogen availability.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Joao R. M. Almeida, Magnus Wiman, Dominik Heer, Daniel P. Brink, Uwe Sauer, Baerbel Hahn-Hagerdal, Gunnar Liden, Marie F. Gorwa-Grauslund
Summary: To evaluate the capability of Saccharomyces cerevisiae to adapt to lignocellulosic hydrolysates, the physiological effect of cultivating yeast in spruce hydrolysate was comprehensively studied. The yeast developed a rapid adaptive response to lignocellulosic hydrolysate, significantly improving its fermentation performance in subsequent experiments. The adaptive response involved the induction of NADPH-dependent aldehyde reductases and conversion of phenolic compounds.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Karen O. Osiro, Daniel P. Brink, Celina Borgstrom, Lisa Wasserstrom, Magnus Carlquist, Marie F. Gorwa-Grauslund
FEMS YEAST RESEARCH
(2018)