Article
Microbiology
Natalia Padilla-Galvez, Paola Luengo-Uribe, Sandra Mancilla, Amandine Maurin, Claudia Torres, Pamela Ruiz, Andres France, Ivette Acuna, Homero Urrutia
Summary: This study aimed to select endophytic actinobacteria from native potatoes in southern Chile for antagonistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum. It was found that Streptomyces sp. TP199 had significant potential as a biological control agent for the prevention of soft rot and blackleg diseases in potato crops.
Article
Agronomy
Xin Zhan, Rui Wang, Manman Zhang, Yuejiao Li, Tao Sun, Jie Chen, Jishun Li, Tong Liu
Summary: The study demonstrated that emodin from Trichoderma asperellum LN004 acts as a quorum sensing inhibitor (QSI) by competitively binding both ExpI and ExpR proteins in Pcc, interfering with its quorum sensing system and reducing the production of virulence factors. The research provides the first molecular mechanism revealing emodin's ability as a QSI to competitively inhibit two QS proteins simultaneously.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Hyeongsoon Kim, Minsik Kim, Sam-Nyu Jee, Sunggi Heu, Sangryeol Ryu
Summary: Pectobacterium carotovorum subsp. carotovorum is a pathogen that causes soft rot disease in crops by producing plant cell wall-degrading enzymes (PCWDEs). The emergence of antibiotic-resistant bacteria and the demand for harmless antimicrobial products have driven the search for alternative therapeutic strategies. In this study, a phage cocktail targeting two different phage receptors was developed to control P. carotovorum subsp. carotovorum. The phage cocktail effectively reduced the populations of P. carotovorum subsp. carotovorum and attenuated its virulence, as demonstrated by decreased PCWDE production and decreased swimming motility in phage-resistant mutants. These results suggest that phage cocktails targeting multiple receptors could be an effective approach for controlling P. carotovorum subsp. carotovorum in crops.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Aparna Anil Singh, Anil Kumar Singh, Anuradha Nerurkar
Summary: Genus Pectobacterium is a major cause of soft rot diseases in fruits and vegetables globally, and there is a need for alternative ecofriendly and economical strategies. Bacillus sp. OA10 extract can effectively inhibit the pathogenicity of PccBR1, potentially by disrupting the QS pathways.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Jin Cai, Shiqin Wang, Yichen Gao, Qi Wang
Summary: This study investigated the antibacterial activity, chemical compounds and antibacterial mechanism of Polygonum orientale L. essential oil (POEO) against Pectobacterium carotovorum subsp. carotovorum (Pcc). The results showed that POEO exhibited effective inhibitory activity against Pcc, making it a potential alternative to chemical bactericides for controlling Pcc.
Article
Agronomy
Weiyao Yan, Xuan Fu, Yuan Gao, Liyin Shi, Qi Liu, Wenchao Yang, Jianguo Feng
Summary: Copper sulfide nanoparticles (CuS NPs) were prepared and evaluated for their antibacterial activity and safety, showing excellent efficacy against Pectobacterium carotovorum subsp. carotovorum (Pcc) and low toxicity to earthworms and cells. CuS NPs could be utilized as a highly effective, lowly toxic, and environmentally friendly antibacterial agent.
PEST MANAGEMENT SCIENCE
(2022)
Article
Microbiology
Ping-Chen Chung, Ruchi Briam James S. Lagitnay, Reymund C. Derilo, Jian-Li Wu, Yutin Chuang, Jia-De Lin, Duen-Yau Chuang
Summary: Carocin S2 is a bacteriocin produced by Pectobacterium carotovorum subsp. carotovorum 3F3 strain with a low molecular weight. Through mutational research, the active sites of Carocin S2 were identified and it was found that the binding between the immunity protein and the bacteriocin was inhibited by an uncharacterized domain.
Article
Biochemistry & Molecular Biology
Bincheng Li, Jiaoli Huang, Youjin Yi, Sisi Liu, Rukuan Liu, Zhihong Xiao, Changzhu Li
Summary: This study evaluated the potential of rhapontigenin as a quorum sensing (QS) inhibitor against Pectobacterium carotovorum subsp. carotovorum (P. carotovorum). The results show that rhapontigenin significantly inhibited the motility, EPS production, biofilm formation, virulence-exoenzyme synthesis and AHL production of P. carotovorum. Moreover, direct-inoculation assay revealed that rhapontigenin could extend the shelf-life of vegetables and prevent the spread of soft-rot symptoms.
Article
Microbiology
Huang-Pin Wu, Reymund C. Derilo, Shih-Hao Hsu, Jia-Ming Hu, Duen-Yau Chuang
Summary: This study reports the discovery, isolation, characterization, and functional analysis of Carocin S4, a novel low-molecular-weight bacteriocin from Pectobacterium carotovorum subsp. carotovorum (Pcc). Carocin S4, composed of CaroS4K and CaroS4I, exhibits deoxyribonuclease activity and hydrolyzes both genomic and plasmid DNA. This study reveals another nuclease type of bacteriocin in Pcc, with CaroS4K and CaroS4I functioning as killer and immunity proteins, respectively.
Article
Biotechnology & Applied Microbiology
Alexey S. Vasilchenko, Darya Poshvina, Roman Yu Sidorov, Aleksandr Iashnikov, Eugene A. Rogozhin, Anastasia V. Vasilchenko
Summary: Bacterial intercellular communication through quorum sensing is a common mechanism for regulating bacterial colonization and survival. This study discovered plant-derived quorum sensing inhibitors from oak bark, which reduced the production of acyl-HSL and decreased bacterial cellulolytic and protease activity. The treatment also suppressed QS-related genes and conferred resistance to soft-rot symptoms in pre-treated potato tubers. The identification of biologically active molecules in the oak bark extract suggests its potential for bacterial control during potato storage.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chung-Pei Chang, Ruchi Briam James Sersenia Lagitnay, Tzu-Rong Li, Wei-Ting Lai, Reymund Calanga Derilo, Duen-Yau Chuang
Summary: This study investigated the role of the catabolite activator protein (CAP) in the regulation of carocin synthesis in Pcc. Knockout of the crp gene resulted in inhibition of genes related to bacteriocin export and affected the production of low-molecular-weight bacteriocins. UV induction influenced the binding preference of CRP to the CAP sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Wei He, Wenfang Luo, Junhui Zhou, Xiafen Zhu, Jianjun Xu
Summary: An outbreak of stem rot in eggplants was observed in Heshuo County, Xinjiang, during winter 2021-2022, affecting 12-35% of the eggplants in the region. Four bacterial strains were isolated from the infected tissues and identified as Pectobacterium carotovorum subsp. brasiliense. Koch's postulates confirmed the pathogenicity of the strains, and a host range test showed their broad spectrum of infectivity. This study reports the first case of stem rot in eggplants caused by P. carotovorum subsp. brasiliense.
Article
Agricultural Engineering
Mojgan Nouri, Sareh Baghaee-Ravari, Bahareh Emadzadeh
Summary: The study showed that nano-emulsified savory and thyme oils have antibacterial properties and can be effective in preventing soft rot disease in potato tubers.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Yanjiao Qi, Mingyang Wang, Bo Zhang, Yue Liu, Jiaqin Fan, Zifan Wang, Li Song, Peer Mohamed Abdul, Hong Zhang
Summary: Rheum tanguticum extract (RTMBE) is found to have antibacterial activity against drug-resistant phytopathogenic bacteria by inhibiting key protein expressions and attenuating their virulence.
Article
Plant Sciences
Lidan Zhang, Tingmi Yang, Ying Zhao, Zhitong Mo, Zhixiang Yuan, Yanchang Yang, Mingzhao Wang, Hongxia Liu
Summary: A bacterial pathogen strain was isolated from susceptible tissue of Hongyang variety kiwifruit in Zhongfeng Town, Ziyuan County, Guilin City, Guangxi, China. The pathogen was identified as Pectobacterium carotovorum subsp. actinidiae through morphological, physiological, biochemical, and genetic analysis. This is the first report of kiwifruit canker disease caused by P. carotovorum subsp. actinidiae in Guangxi, China. The study provides a preliminary understanding of the pathogen, which will contribute to future disease prevention and control.
Article
Microbiology
Xinxin He, Ronghua Liu, Jinchang Liang, Yuying Li, Xiuxiu Zhao, Lingman Ran, Waqar Ahmad, Xiao-Hua Zhang
Summary: The bacterium strain ZXX205(T) isolated from the Mariana Trench at a depth of 7500 m in the Pacific Ocean is a new species identified as Winogradskyella ouciana within the genus Winogradskyella.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yunhui Zhang, Peng Yao, Chuang Sun, Sanzhong Li, Xiaochong Shi, Xiao-Hua Zhang, Jiwen Liu
Summary: This study examined the microbial populations in subseafloor sediment cores from the South China Sea, providing insights into the vertical changes of microbial diversity and the importance of rare species in co-occurrence networks. The research highlighted the distinct responses of rare species to environmental factors and the potential roles they play in marine benthic ecosystems.
Article
Multidisciplinary Sciences
Jiaqi Liu, Haibo Zhou, Zhiyu Yang, Xue Wang, Hanna Chen, Lin Zhong, Wentao Zheng, Weijing Niu, Sen Wang, Xiangmei Ren, Guannan Zhong, Yan Wang, Xiaoming Ding, Rolf Mueller, Youming Zhang, Xiaoying Bian
Summary: The study rationalized the construction of genome-reduced S. brevitalea mutants, enabling higher yields of heterologous expression and identification of new compounds from Chitinimonas koreensis.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Hao Sun, Ji Liu, Siyin Tan, Yanfen Zheng, Xiaolei Wang, Jinchang Liang, Jonathan D. Todd, Xiao-Hua Zhang
Summary: In the Changjiang Estuary, bacterial DMSP producers and their related genes show higher abundance and transcription levels in seawater samples compared to freshwater samples along the salinity gradient, with a more prominent presence in summer than in winter samples. DMSP cleavage is likely the dominant catabolic pathway, with DMSP lyase genes being significantly more abundant than the demethylase gene dmdA. This study serves as a model for future research on microbial DMSP cycling in estuarine environments.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Mengdan Xing, Yan Wang, Yujuan Zhao, Zhe Chi, Zhenming Chi, Guanglei Liu
Summary: In this study, a C-terminal bacterial immunoglobulin-like domain (Big_2) was identified in kappa-carrageenase and found to be essential for maintaining the activity and thermostability of the enzyme. The noncatalytic Big_2 domain was also shown to contribute to the processivity of the enzyme and could enhance hydrolysis of kappa-carrageenan independently. Additionally, sequence analysis revealed the conservation of Big_2 domains in bacterial kappa-carrageenases, suggesting their universal noncatalytic functions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Jinchang Liang, Jiwen Liu, Xiaolei Wang, Hao Sun, Yulin Zhang, Feng Ju, Fabiano Thompson, Xiao-Hua Zhang
Summary: This study compared the genomes of five Vibrio campbellii strains isolated from different depths of the Mariana and Yap Trenches and identified genomic characteristics that facilitate survival in the deep sea. Pelagic strains showed genome streamlining, while deep-sea strains had more genes involved in amino acid metabolism, cell wall biogenesis, and ion transport and metabolism. Deep-sea strains also had specific antibiotic resistance and substrate degradation genes. In contrast, pelagic strains had the ability for assimilatory nitrate reduction.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biology
Keke Zhang, Zhijian Li, Qiaoyun Zhu, Huansheng Cao, Xinxin He, Xiao-Hua Zhang, Weizhi Liu, Qianqian Lyu
Summary: Characterization of PL7 alginate lyase structure and products enables a bioinformatics approach to predict product distribution from the amino acid sequence.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jiwen Liu, Yanlu Qiao, Yu Xin, Yang Li, Xiao-Hua Zhang
Summary: This study provides insights into the temperature response pattern and disruptive effects of temperature change on marine microbial communities. The results show that temperature plays a critical role in shaping the seasonal dynamics of microbial communities, and even small temperature changes can lead to significant community turnover. The temperature response pattern is phylogenetically structured, indicating that closely related taxa may have contrasting responses to temperature fluctuations.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xinxin He, Yunhui Zhang, Xiaolei Wang, Xiaoyu Zhu, Leiran Chen, Weizhi Liu, Qianqian Lyu, Lingman Ran, Haojin Cheng, Xiao-Hua Zhang
Summary: Alginate is an important carbon source in the marine environment and vibrios are major utilizers. However, little is known about the comprehensive alginate-degrading strategy in vibrios. In this study, a highly efficient alginate-degrading Vibrio strain was characterized and its molecular mechanisms were elucidated. The strain possessed 11 distinct alginate lyases and three alginate utilization loci. Importantly, the involvement of outer membrane vesicles in the secretion of alginate lyases was proposed for the first time. These findings provide insights into the specific alginate degradation strategy in Vibrio and highlight the ecological importance of efficient alginate-degrading vibrios in the ocean.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiaolei Wang, Jiwen Liu, Wenbin Zhao, Jinmei Liu, Jinchang Liang, Fabiano Thompson, Xiao-Hua Zhang
Summary: Vibrio spp. populations in the Chinese marginal seas show distinct distribution patterns and community assembly mechanisms, influenced by spatial scale and stochastic processes. The dominant Vibrio spp. groups vary among different geographical locations, and random processes play a significant role in shaping the geographical pattern of Vibrio communities.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Pingping Zhang, Xiaolei Wang, Ronghua Liu, Xiaoyu Zhu, Wenbin Zhao, Xiao- Hua Zhang
Summary: Two strains of Gram-stain-negative, oxidase-positive, facultative anaerobic, rod-shaped motile bacteria, named ZSDZ34 and ZSDE26, were isolated from offshore surface seawater near Qingdao. Phylogenetic analysis based on 16S rRNA gene sequences placed ZSDZ34T and ZSDE26T within the genus symbolscript family symbolscript class symbolscript. ZSDE26T showed the highest sequence similarity (97.3%) with symbolscript symbolscript VB 8.9T, while ZSDZ34T showed the highest sequence similarity (97.8%) with symbolscript symbolscript subsp. symbolscript DSM 109723T.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Haojin Cheng, Yunhui Zhang, Zihua Guo, Xinxin He, Ronghua Liu, Xiao-Yu Zhu, Jiake Li, Jiwen Liu, Xiao-Hua Zhang
Summary: This study reveals the DMSP content and metabolic bacterial groups in the subseafloor sediments of the Mariana Trench. The vertical variation trend of DMSP content in the Mariana Trench sediment is distinct from that of the continental shelf sediment. Although dsyB and dddP are the dominant DMSP synthetic and catabolic genes in the Mariana Trench sediment, multiple previously unknown DMSP metabolic bacterial groups, especially anaerobic bacteria and actinomycetes, are discovered through metagenomic and culture methods. The active conversion of DMSP, DMS, and methanethiol may also occur in the Mariana Trench sediments.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Wenbin Zhao, Leiran Chen, Mei Liu, Keyi Huang, Yunqi Ding, Jiaguang Xiao, Peng Tian, Jiwen Liu, Xiao-Hua Zhang, Wentao Niu, Xiaolei Wang
Summary: This study investigated the distribution pattern and interactions of total bacteria and Vibrio spp. in the sediments from Xisha Islands during the coral bleaching event of 2020. The results showed that the abundances of Vibrio were high, indicating the bloom of sedimentary Vibrio spp. Coral pathogenic Vibrio species were abundant in the sediments, likely reflecting adverse effects on several kinds of corals. The compositions of the Vibrio spp. were separated by geographical location, which was mainly attributable to the spatial distance and coral species.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Ruihong Guo, Yalin Yuan, Ronghua Liu, Wen Hong, Yi Zhou, Wenbin Zhao, Xiaolei Wang, Xiao-Hua Zhang
Summary: Two novel strains of marine bacteria, designated DBSS07T and ZSDZ65T, were isolated from different marine environments. Phylogenetic analysis revealed their placement within the genus Vibrio. The strains exhibited different growth conditions and fatty acid compositions, prompting the proposal of two new species within the genus Vibrio: V. paucivorans sp. nov. (type strain DBSS07T) and V. qingdaonensis sp. nov. (type strain ZSDZ65T).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Yuechao Zhang, Qinqin Hao, Oumei Wang, Xiao-Hua Zhang, Fanghua Liu
Summary: Microbial iron reduction plays a crucial role in marine ecosystems, contributing to element cycling and supporting biological activities. Fermentative iron reduction exhibits a pH-buffering effect and improves glucose consumption, bacterial growth, and metabolite production.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2023)
