Journal
KIDNEY INTERNATIONAL
Volume 96, Issue 3, Pages 689-698Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.kint.2019.04.023
Keywords
DSA; endothelium; IL-6; kidney transplantation; rejection; Treg
Categories
Funding
- Societe Francophone de la Transplantation
- Agence de la Biomedecine
- Vaincre la Mucoviscidose
Ask authors/readers for more resources
Development of donor-specific antibodies is associated with reduced allograft survival in renal transplantation. Recent clinical studies highlight the prevalence of human leukocyte antigen (HLA)-DQ antibodies amongst de novo donor-specific antibodies (DSAs), yet the specific contribution of these DSAs to rejection has not been examined. Antibody-mediated rejection primarily targets the microvasculature, so this study explored how patient HLA-DQ alloantibodies can modulate endothelial activation and so immunoregulation. HLA-DQ antibodies phosphorylated Akt and S6 kinase in microvascular endothelial cells. This activation prior to culture with alloreactive lymphocytes increased IL-6 and RANTES secretion. The antibody-mediated upregulation of IL-6 was indeed Akt-dependent. The binding of HLA-DQ antibodies to endothelial cells selectively reduced T cell alloproliferation and FoxP3(high) Treg differentiation. In clinical studies, detection of HLA-DQ DSAs with other DSAs is associated with worse graft survival than either alone. Endothelial cells stimulated with HLA-DR and HLA-DQ antibodies showed a synergistic increase in proinflammatory cytokine secretion and a decrease in Treg expansion. HLA-DQ antibodies strongly promote proinflammatory responses in isolation and in combination with other HLA antibodies. Thus, our data give new insights into the pathogenicity of HLA-DQ DSAs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available