Article
Chemistry, Physical
Jing Ren, Jinghan Chi, Baishi Wang, Lingli Guo, Yudi Han, Xinhui Liu, Xuetao Pei, Wen Yue, Yan Han
Summary: This study demonstrated that the combination of human adipose-derived stem cells (hASCs) and a human decellularized adipose tissue matrix (hDAM) could accelerate wound healing and promote angiogenesis, showing potential for clinical application.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Review
Dermatology
Tian Yuan, Li Meijia, Chen Xinyao, Chen Xinyue, Hao Lijun
Summary: Exosomes derived from human adipose-derived stem cells (ADSCs) have shown excellent capability in improving wound healing quality, but their efficacy in clinical applications is still uncertain. This study conducted a systematic review of preclinical studies comparing exosomes from human ADSCs with placebo in animal models of wound closure. The results showed that administration of exosomes derived from human ADSCs significantly improved wound closure compared to controls, with the same effect as ADSCs. The therapeutic effect was further enhanced by modified ADSC-EV. This suggests that exosomes derived from human ADSCs, especially after enrichment for specific non-coding RNA, have promising potential to improve healing efficiency.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Yunchuan Wang, Yue Zhang, Ting Li, Kuo Shen, Ke Jia Wang, Chenyang Tian, Dahai Hu
Summary: Engineered skin substitutes derived from human skin reduce inflammatory reactions and are easier to use in clinics. Type I collagen and platelet-rich plasma are mixed to form a stable scaffold, while adipose mesenchymal stem cell derived exosomes improve the performance of the engineered skin. This method provides a new therapeutic strategy and theoretical basis for tissue regeneration and wound repair.
ADVANCED MATERIALS
(2023)
Review
Cell Biology
Ning Zeng, Hongbo Chen, Yiping Wu, Zeming Liu
Summary: Wound healing in the human body is a complex physiological regulation mechanism, with adipose stem cells playing an important role in regenerative medicine. Adipose stem cells are easy to obtain, high-yielding, rich in components and cytokines, and highly practical.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Health Care Sciences & Services
Meenakshi Suku, Ashang Luwang Laiva, Fergal J. O'Brien, Michael B. Keogh
Summary: The study shows that pre-treatment with beta-klotho protein promotes inflammatory signal pruning, enhances the pro-angiogenic response of diabetic stem cells, and accelerates the cellular expression of matrix proteins, effectively speeding up the functional development of diabetic stem cells for wound healing applications.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Chia-Fen Hsieh, Chih-Hao Chen, Hao-Hsi Kao, Darshan Tagadur Govindaraju, Banendu Sunder Dash, Jyh-Ping Chen
Summary: By incorporating hyaluronic acid (HA) into electrospun poly (lactide-co-glycolide) (PLGA)/gelatin (PG) fibrous membrane scaffolds (FMS), PLGA/gelatin/HA (PGH) FMS was fabricated for 3D culture and delivery of adipose-derived stem cells (ASCs). PGH/ASC treatment accelerates wound healing in rats by modulating immune reaction, promoting angiogenesis, and reducing scar formation at the wound sites.
Article
Engineering, Biomedical
Dequan Zhang, Qiang Fu, Huijuan Fu, Jinshi Zeng, Litao Jia, Minliang Chen
Summary: 3D-printed bioactive scaffolds produced through 3D printing can provide an individualized approach to treating full-thickness skin defects. Adipose tissues obtained by liposuction are rich in adipose-derived bioactive materials, which can support wound healing.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Cell & Tissue Engineering
Yang Zhou, Bo Zhao, Xin-Liao Zhang, Yi-jun Lu, Shou-Tao Lu, Jian Cheng, Yu Fu, Lin Lin, Ning-Yan Zhang, Pei-Xin Li, Jing Zhang, Jun Zhang
Summary: This study investigated the potential clinical application roles of human adipose-derived mesenchymal stem cells (hADSC) and hADSC-derived exosomes (hADSC-Exo) in cutaneous wound healing. The combined administration of local hADSC-Exo smearing and hADSC/hADSC-Exo intravenous administration showed additional benefits in promoting wound healing, accelerating re-epithelialization, reducing scar widths, and enhancing angiogenesis and collagen synthesis.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Immunology
Qingyi Jia, Hanxing Zhao, Yixi Wang, Ying Cen, Zhenyu Zhang
Summary: Wound healing is a complex process that involves multiple phases, including inflammation, proliferation, and remodeling. Abnormal inflammation can impede wound healing under certain pathological conditions. Adipose-derived stem cells (ADSCs) and their secreted extracellular vesicles (ADSC-EVs) have shown potential in promoting wound healing by inhibiting abnormal inflammation responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Jiachao Xiong, Hong Qiang, Ting Li, Jiayi Zhao, Ziyu Wang, Fei Li, Jianwen Xu
Summary: The study demonstrates that human adipose-derived stem cells can promote the vascularization of seawater-treated wounds by differentiating into vascular endothelial cells and promoting their proliferation and migration. Bioinformatics analysis identified genes that may regulate wound healing mechanisms such as extracellular matrix remodeling, programmed cell death, inflammation, and vascularization.
Article
Dermatology
Ali Reza Khalatbary, Melody Omraninava, Davood Nasiry, Mitra Akbari, Saeid Taghiloo, Mahnaz Poorhassan, Roohollah Ebrahimpour-Malekshah, Mahdiyeh Asadzadeh, Amir Raoofi
Summary: This study investigated the effect of adipose-derived stem cells (ADSCs)-derived exosomes loaded bioengineered micro-porous three-dimensional amniotic membrane-scaffold (AMS) on healing in diabetic rats. The results showed that the treated groups, especially the AMS + Exo group, had significantly higher wound closure rate, volumes of newly formed epidermis and dermis, density of fibroblasts and proliferating cells, blood vessel length density, collagen density, and tensiometrical parameters compared to the control group. The expression of TGF-β, bFGF, and VEGF genes was significantly upregulated in all treated groups, while the expression of TNF-α and IL-1β, as well as cell densities of neutrophils, M1 macrophages, and mast cells, decreased more in the AMS + Exo group.
ARCHIVES OF DERMATOLOGICAL RESEARCH
(2023)
Article
Anatomy & Morphology
Davood Nasiry, Ali Reza Khalatbary, Afshin Noori, Beheshteh Abouhamzeh, Zahra Jamalpoor
Summary: The lack of suitable scaffold and blood supply, as well as the shortage of growth factors, are the main factors contributing to non-optimal healing of diabetic wounds. In this study, a combination of a bioengineered decellularized amniotic membrane-scaffold and adipose-derived stem cells (ASCs) was investigated for its potential in promoting healing in ischemic wounds in type 1 diabetic rats. The results showed that the treatment groups had significantly higher rates of wound closure, volumes of new epidermis and dermis, densities of fibroblasts and blood vessels, numbers of proliferating cells and collagen deposition, as well as better biomechanical properties of the healed wounds compared to the untreated group. The DAMS+ASC group exhibited the most favorable outcomes. Overall, using both DAMS engraftment and ASC transplantation had a greater impact on diabetic wound healing.
Article
Cell & Tissue Engineering
Christian Ploner, Tina Rauchenwald, Catherine E. Connolly, Karin Joehrer, Johannes Rainer, Christof Seifarth, Martin Hermann, Markus Nagl, Susanne Lobenwein, Doris Wilflingseder, Giuseppe Cappellano, Evi M. Morandi, Gerhard Pierer
Summary: The presence of adipocytes in wound tissue indicates a prevailing anti-inflammatory environment that can be promoted by NCT treatment, and may be associated with improved healing outcomes.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Nanoscience & Nanotechnology
Jing Li, Hong Gao, Yue Xiong, Ling Wang, Haojie Zhang, Fumei He, Jingxin Zhao, Shuna Liu, Liqian Gao, Ying Guo, Wenbin Deng
Summary: This study found that exosomes derived from neural stem cells may enhance wound healing through neurotrophic factors and immunomodulation.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Chang Sik Pak, Chan Yeong Heo, Jisoo Shin, Soo Young Moon, Seung-Woo Cho, Hyo Jin Kang
Summary: The combination of HA-CA and ADSCs showed synergistic effects in diabetic wound healing, providing significant healing effects by maximizing paracrine signaling and angiogenesis. This combination might represent a novel strategy for the treatment of diabetic wounds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Chen-Chie Wang, Li-Chun Wang, Kai-Chiang Yang, Miin-Jang Chen, Hsin-Chih Lin, Yin-Yi Han
Summary: This study demonstrated that deposition of aluminum oxide (Al2O3) films by thermal atomic layer deposition (T-ALD) can improve anticoagulation of the PVC tubing system. T-ALD had a relative hydrophilicity compared with blank PVC and plasma-enhanced ALD (PE-ALD). Whole blood immersion tests showed that blood clots formed on blank PVC and a large amount of red blood cells was found on PE-ALD substrates, whereas less blood cells were noted in T-ALD samples.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Medicine, General & Internal
Ming-Hsu Chen, Wei-Chiu Tai, Nai-Chen Cheng, Ching-He Chang, Po-Chun Chang
Summary: This study verified the regeneration capability of mucosal connective tissue stromal cells and found that alveolar mucosa cells contain a high percentage of stem/progenitor cells and show differentiation capability toward osteogenic lineage.
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION
(2022)
Article
Materials Science, Multidisciplinary
Pi-Chen Lin, Kaifan Lin, Yu-Hsuan Lin, Kai-Chiang Yang, Vladimir Ivanovitch Semenov, Hsin-Chih Lin, Miin-Jang Chen
Summary: Polycrystalline HfZrO2 (HZO) film can enhance the corrosion resistance and reduce the corrosion rate of as-extruded Mg-Ca alloy. Compared to ZrO2 film, HZO film exhibits better short-term corrosion resistance. HZO film shows low toxicity and is suitable for the protective coating of biological implants.
Article
Engineering, Biomedical
Chih-Yung Chiang, Wei-Chia Chang, Wei-Min Chang, Yin-Chuan Shih, Feng-Huei Lin, Chang-Chin Wu, Kai-Chiang Yang
Summary: The addition of cefazolin changed the characteristics of calcium sulfate bone cement, including prolonging setting time, impairing mechanical strength, accelerating degradation, and causing cytotoxicity. Moreover, it also altered the crystalline structure and morphology of calcium sulfate.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Che-Wei Lin, Po-Ting Wu, Er-Yuan Chuang, Yu-Jui Fan, Jiashing Yu
Summary: Soy protein-based composite scaffolds, blended with tapioca starch and gellan gum, show great potential in biomedical and tissue engineering applications. The adjustable properties and biocompatibility of these scaffolds promote cell adhesion, proliferation, and migration, as well as enhance wound healing processes.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ming-Hsiao Hu, Kai-Chiang Yang, Chih-Wei Chen, Po-Han Chu, Yun-Liang Chang, Yuan-Hui Sun, Feng-Huei Lin, Shu-Hua Yang
Summary: In this study, a multilayer electrospun fibroin/gelatin scaffold mimicking the lamellae structure of the native annulus fibrosus was developed and evaluated. The scaffold exhibited biocompatibility, maintained cell viability and colonization, and induced collagen fibrous tissue production within the annulus fibrosus defect. Compared to the untreated group, the implant group showed superior effectiveness in annulus repair.
Article
Biochemistry & Molecular Biology
Wu-Xun Chen, Nai-Chen Cheng, Yan-Jun Chen, I-Chi Lee
Summary: In this study, silk fibroin-based microneedle patches were fabricated as carriers for adipose stem cell recruitment. The crystallinity of silk fibroin-based microneedles was determined after water annealing treatment. The results showed that the microneedle patches had sufficient strength to penetrate the skin and demonstrated sustained release of the model drug. Dual-layer microneedles loaded with SDF-1 were prepared to enhance drug release for stem cell recruitment.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell & Tissue Engineering
Che-Chang Tu, Nai-Chen Cheng, Jiashing Yu, Yi-Xuan Pan, Wei-Chiu Tai, Yin-Chuan Chen, Po-Chun Chang
Summary: This study evaluates the potential of self-assembled adipose-derived stem cell spheroids (ADsp) in microbial transglutaminase cross-linked gelatin hydrogel (mTG) for treating diabetic periodontal wounds and craniofacial defects. The results show that osteogenically pretreated ADsp-mTG can promote the regeneration of diabetic osseous defects. This suggests that ADsp-mTG can facilitate diabetic periodontal wound healing and craniofacial osseous defect regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
Chia-Hsiang Yen, Nai-Chen Cheng, Hao-Ying Hsieh, Ching-Wen Tsai, An-Li Lee, Chien-Yi Lu, Yin-Tzu Chen, Tai-Horng Young
Summary: This study developed a continuous cell production system by blending pH-responsive chitosan (CS) with polyamide-66 (PA) to mass-produce adipose-derived stem cells (ASCs) with sustained regenerative capacity. The PA17CS blend out-performed other blends in terms of cell detachment ratio and cell viability. ASCs harvested from PA17CS exhibited superior stemness characteristics and demonstrated effective neovascularization and protective effects in a rat model.
MATERIALS TODAY BIO
(2023)
Article
Nanoscience & Nanotechnology
Yu-Sheng Hsiao, Edgar Daniel Quinones, Shih-Chieh Yen, Jiashing Yu, Ji-Tseng Fang, Peilin Chen, Ruey-Shin Juang
Summary: Electric cell-substrate impedance sensing (ECIS) is an innovative approach for the label-free and real-time detection of cell morphology, growth, and apoptosis. In this study, poly(3,4-ethylenedioxythiophene)-poly-(styrenesulfonate) (PEDOT:PSS) conducting polymer (CP)-based bioelectrodes were developed for the simultaneous drug release and real-time biosensing of cancer cell viability. This device can reliably monitor impedance signal changes correlated with cell viability while exhibiting excellent properties for both drug encapsulation and on-demand release.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Kuan-Ting Liu, Edgar Daniel Quinones, Ming-Hsin Liu, Che-Wei Lin, Yan-Ting Chen, Chia-Che Chiang, Kevin Chia-Wen Wu, Yu-Jui Fan, Er-Yuan Chuang, Jiashing Yu
Summary: Targeted thrombolysis using GCPIH nanoparticles is of great significance in overcoming the limitations of short circulation time and off-target hemorrhage.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Chun-Ting Liu, Jiashing Yu, Min-Hsuan Lin, Kai-Hsiang Chang, Che-Yu Lin, Nai-Chen Cheng, Po- Wu, Chun-Wei Huang, Pin-Yu Zhang, Min-Tzu Hung, Yu-Sheng Hsiao
Summary: The investigation of electrical and mechanical stimulations on chondrogenesis in tissue engineering is crucial for successful cartilage repair and regeneration. Electrical stimulation promotes MSC chondrogenesis, while mechanical stimulation enhances the chondrogenic differentiation capacity of MSCs. Understanding the impact of these stimuli is essential for developing effective tissue engineering strategies.
Article
Cell & Tissue Engineering
Chun-Wei Li, Tai-Horng Young, Mu-Hui Wang, Ming-Ying Pei, Tsung-Yu Hsieh, Chia-Lang Hsu, Nai-Chen Cheng
Summary: Our study demonstrates that a diabetic environment has an adverse effect on ASCs, and adopting a low glucose culture condition is a simple and effective way to enhance the wound healing capabilities of diabetic ASCs. This finding is valuable for the clinical application of autologous ASCs from diabetic patients.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
Thu-Trang Nguyen, Chih-Chien Hu, Rajalakshmi Sakthivel, Sasza Chyntara Nabilla, Yu-Wen Huang, Jiashing Yu, Nai-Chen Cheng, Yi-Jie Kuo, Ren-Jei Chung
Summary: This study developed a collagen/gamma-polyglutamate acid/hydroxyapatite composite scaffold using 3D printing technology. A collagen/gamma-PGA/hydroxyapatite 2D membrane was also fabricated for comparison. In-vitro and in-vivo experiments showed that the scaffold has good biodegradation and biocompatibility properties, and the collagen-hydroxyapatite scaffold enhances the mechanical properties for osteochondrogenesis.
BIOMATERIALS RESEARCH
(2022)
