Journal
JOURNAL OF PHYSICS D-APPLIED PHYSICS
Volume 52, Issue 26, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/1361-6463/ab16b4
Keywords
temporal focusing; multiphoton microscopy; quantum dots; fluorescence microscopy; neurophotonics
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Funding
- Wellcome Trust [093831/B/10/Z]
- NIH (Laser Biomedical Research Center) [9-P41-EB015871-26A1]
- European Molecular Biology Organization (EMBO) long-term fellowship
- NIH [5-U54-CA151884, P01-CA080124, R01-CA126642, R01-CA096915]
- NCI/Federal Share Proton Beam Program Income
- ARO through the Institute for Soldier Nanotechnologies [W911NF-13-D-0001]
- Department of Defense [DoD W81XWH-10-1-0016]
- NSF [ECCS-1449291]
- National Institute of Health [5R01NS051320, 4R44EB012415, 1R01HL121386-01A1]
- National Science Foundation [CBET-0939511]
- Hamamatsu Corporation
- Singapore-Massachusetts Institute of Technology Alliance for Research and Technology (SMART) Center, BioSystems and Micromechanics (BioSyM)
- Wellcome Trust [093831/B/10/Z] Funding Source: Wellcome Trust
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The first ever demonstration of temporal focusing with short wave infrared (SWIR) excitation and emission is demonstrated, achieving a penetration depth of 500 mu m in brain tissue. This is substantially deeper than the highest previously-reported values for temporal focusing imaging in brain tissue, and demonstrates the value of these optimized wavelengths for neurobiological applications.
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