Article
Plant Sciences
Dinesh Adhikary, Devang Mehta, R. Glen Uhrig, Habibur Rahman, Nat N. V. Kav
Summary: This study used proteomics to investigate the interaction between P. brassicae pathotype 3 and canola, revealing the involvement of proteins related to calcium signaling, reactive oxygen species (ROS) biochemistry, thaumatin, and other factors. In addition, several proteins orthologous to known clubroot resistance (CR) genes were identified. This research improves our understanding of the protein-level mechanisms involved in canola's response to P. brassicae and provides insights for developing CR cultivars.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Homa Askarian, Alireza Akhavan, Leonardo Galindo Gonzalez, Sheau-Fang Hwang, Stephen E. Strelkov
Summary: The study found a clear relationship between the genetic structure and virulence phenotypes of the clubroot pathogen, with low genetic variability within each pathotype group but significant genetic differentiation among the pathotypes. Matrices produced with genetic data and the hosts of the CCD set showed the highest correlation between genetic structure and virulence.
Article
Microbiology
Leonardo Galindo-Gonzalez, Sheau-Fang Hwang, Stephen E. Strelkov
Summary: Clubroot is a major disease in Canadian canola caused by Plasmodiophora brassicae, with efforts focused on developing host resistance against new resistance-breaking pathotypes. Limited research has been done on investigating the molecular mechanisms of pathogenic infection due to its obligate biotrophic nature.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Xuefei Jiang, Ying Su, Maolin Wang
Summary: In this study, a clubroot disease resistance (CR) gene locus was identified and a key gene, BnERF034, was revealed. This study provides a theoretical basis for understanding the molecular mechanisms underlying CR in plants and resistance breeding in Brassicaceae crops.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Piotr M. Kopec, Katarzyna Mikolajczyk, Ewa Jajor, Agnieszka Perek, Joanna Nowakowska, Christian Obermeier, Harmeet Singh Chawla, Marek Korbas, Iwona Bartkowiak-Broda, Wojciech M. Karlowski
Summary: Clubroot, caused by Plasmodiophora brassicae infection, is a significant disease affecting cruciferous crops, with limited effective protection measures available. Breeding resistant varieties remains crucial in disease control, despite the presence of multiple described resistance loci that require ongoing updates to avoid resistance breakdowns.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Swati Megha, Zhengping Wang, Nat N. Kav, Habibur Rahman
Summary: In this study, 43 Biotin carboxyl carrier protein (BCCP) genes were identified and analyzed in five Brassica species. The results suggest that these genes may play important roles in stress tolerance in plants. This study provides a foundation for improving stress tolerance in Brassica crops.
Article
Microbiology
A. Michael Klueken, Yamen Mahfoud, Sabine Roessler, Jutta Ludwig-Mueller
Summary: This study demonstrates the positive effects of isotianil and B. amyloliquefaciens QST713-HiCFU on reducing clubroot disease in cruciferous crops. The efficacy is influenced by seasons, host plant genotype, and clubroot isolates. Seed treatments are more effective than drench applications. The combination of isotianil and B. amyloliquefaciens QST713-HiCFU can enhance the efficacy in some cases.
Article
Plant Sciences
Charitha P. A. Jayasinghege, Jocelyn A. Ozga, Victor P. Manolii, Sheau-Fang Hwang, Stephen E. Strelkov, Martin Cerny, Veronika Hyskova
Summary: This study investigated the effect of clubroot, a soilborne disease caused by Plasmodiophora brassicae, on plant hormones in Brassica napus. The results showed that the pathogen modulated plant hormonal networks, leading to increased levels of SA and ABA in susceptible plants, and decreased levels of JA. This modulation of plant hormones could be important for understanding plant defense mechanisms against clubroot.
Article
Plant Sciences
Andrea Botero-Ramirez, Sheau-Fang Hwang, Stephen E. Strelkov
Summary: The study evaluated the effect of clubroot disease on canola yield, with results showing that disease severity and incidence increased with higher inoculum concentrations, with susceptible cultivars exhibiting the highest disease levels and resistant cultivars showing lower disease severity. Yield, pods per plant, and 1000-grain weight decreased with increasing disease severity.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Microbiology
Nazanin Zamani-Noor, Johann Hornbacher, Christel Joy Comel, Jutta Papenbrock
Summary: The study revealed variations in total and individual glucosinolates in roots and leaves of different oilseed rape cultivars following inoculation with various Plasmodiophora brassicae isolates. Susceptible cultivars showed lower glucosinolate contents compared to resistant ones, indicating a potential interaction of breakdown products of glucobrassicin with auxin signaling.
Article
Plant Sciences
Yoann Aigu, Stephanie Daval, Kevin Gazengel, Nathalie Marnet, Christine Lariagon, Anne Laperche, Fabrice Legeai, Maria J. Manzanares-Dauleux, Antoine Gravot
Summary: This study aimed to investigate the mechanisms by which nitrogen fertilization influences clubroot disease. The results showed that clubroot infection led to an increase in SA concentration and induction of SA gene expression, while nitrogen-driven resistance was independent of SA signaling, soluble carbohydrate, and amino acid concentrations. The low-nitrogen-driven resistance was associated with transcriptional regulation of specific genes, including NRT2 and NR genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Usha Rani Patar, Marie Manasova, Jonas Hnatek, Jana Wenzlova, Miloslav Zouhar
Summary: The effectiveness of ethanedinitrile (EDN) against Plasmodiophora brassicae, the causative agent of clubroot disease, was evaluated in this study. The results showed that EDN exhibited good efficacy against the pathogen, with complete disease control achieved at certain rates and durations of exposure. Additionally, EDN improved plant health by increasing shoot weight.
Article
Biochemistry & Molecular Biology
Huishan Liu, Chinedu Charles Nwafor, Yinglan Piao, Xiaonan Li, Zongxiang Zhan, Zhongyun Piao
Summary: In this study, circRNA-seq was performed to investigate the role of circRNAs in clubroot disease resistance in Brassica rapa. Differentially expressed circRNAs were identified and a circRNA-miRNA-mRNA network was constructed. One upregulated circRNA was found to suppress the expression of a miRNA and upregulate a gene, possibly leading to plant resistance. These findings provide new insights into clubroot resistance mechanisms and lay the foundation for further studies on complex gene regulation networks in B. rapa.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Lin Ding, Hu Zhou, Hai-di Liang, Lin Tan, Hui Zhao, Xiao-jun Chen, Zuo-hua Ren
Summary: In this study, a bacterium strain X216 with strong antagonistic ability against Plasmodiophora brassicae was isolated from oilseed rape fields. The strain showed high lethality to the resting spores of P. brassicae and could effectively control clubroot disease on oilseed rape. This research provides a promising option for biological control of oilseed rape clubroot.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Nazanin Zamani-Noor, Imke Krohne, Birger Koopmann
Summary: The resistance of oilseed rape (OSR) cultivars to clubroot is mainly dependent on a major resistance gene from the cv. Mendel. The greenhouse experiments showed significant differences in resistance parameters among different cultivars, with the threshold of inoculum density for disease occurrence strongly influenced by the virulence of the pathogen and susceptibility of the host plant.
Article
Biochemical Research Methods
Saadia Ouzia, Anne-Lise Royer, Marzia Pezzolato, Alessandro Benedetto, Elena Biasibetti, Yann Guitton, Bruno Le Bizec, Elena Bozetta, Gaud Dervilly
Summary: Through a study using reversed phase liquid chromatography-electrospray ionization-high-resolution mass spectrometry, specific patterns and candidate biomarkers associated with three steroid treatments in bovine urine profiles were identified. Level-1 identification of two biomarkers related to energy pathways and skeletal muscle functioning was achieved through MS-2 structural elucidation.
DRUG TESTING AND ANALYSIS
(2022)
Article
Plant Sciences
Anne Levrel, Jocelyne Lemoine, Magali Ermel, Youssef Abu-Ahmad, Antoine Gravot, Regine Delourme
Summary: A cut-petiole inoculation method coupled with visual and PCR-based phenotyping was used to evaluate quantitative resistance (QR) to Leptosphaeria maculans in Brassica napus. This method allows for a faster evaluation of QR and provides insights into the dynamics of pathogen growth. The results obtained were consistent with field phenotyping data and enabled detection of the effect of introduced resistance QTL alleles.
CANADIAN JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Biochemical Research Methods
Chloe Cloteau, Gaud Dervilly, Zied Kaabia, Florian Bagilet, Vivian Delcourt, Benoit Loup, Yann Guitton, Anne-Lise Royer, Fabrice Monteau, Patrice Garcia, Marie-Agnes Popot, Bruno Le Bizec, Ludovic Bailly-Chouriberry
Summary: A non-targeted metabolomics approach was used to identify potential biomarkers associated with the abuse of Anabolic-Androgenic Steroids in animal competitions using equine urine samples. The model's performances and robustness were successfully preserved in a high-throughput liquid chromatography-selected reaction monitoring method. The transferability of the method to a second laboratory demonstrated its applicability in developing biomarker-based strategies for doping control.
DRUG TESTING AND ANALYSIS
(2022)
Article
Environmental Sciences
Komodo Matta, Tiphaine Lefebvre, Evelyne Vigneau, Veronique Cariou, Philippe Marchand, Yann Guitton, Anne-Lise Royer, Stephane Ploteau, Bruno Le Bizec, Jean-Philippe Antignac, German Cano-Sancho
Summary: The study confirmed positive associations between certain persistent organic pollutants and the risk of endometriosis, utilizing various analytical methods to identify the effects of POPs on the disease.
ENVIRONMENT INTERNATIONAL
(2022)
Article
Plant Sciences
Yoann Aigu, Stephanie Daval, Kevin Gazengel, Nathalie Marnet, Christine Lariagon, Anne Laperche, Fabrice Legeai, Maria J. Manzanares-Dauleux, Antoine Gravot
Summary: This study aimed to investigate the mechanisms by which nitrogen fertilization influences clubroot disease. The results showed that clubroot infection led to an increase in SA concentration and induction of SA gene expression, while nitrogen-driven resistance was independent of SA signaling, soluble carbohydrate, and amino acid concentrations. The low-nitrogen-driven resistance was associated with transcriptional regulation of specific genes, including NRT2 and NR genes.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Maykel Hernandez-Mesa, Luca Narduzzi, Sadia Ouzia, Nicolas Soetart, Laetitia Jaillardon, Yann Guitton, Bruno Le Bizec, Gaud Dervilly
Summary: Recent epidemiological studies have shown that current levels of exposure to polychlorinated biphenyls (PCBs) remain a significant health concern, especially for non-dioxin-like PCB exposure. This study used pigs as an animal model to investigate the effects of Aroclor 1260 exposure, resulting in the bioaccumulation of NDL-PCBs in pig fat and the identification of biomarkers related to PCB exposure through metabolomics and lipidomics.
Article
Agriculture, Multidisciplinary
Anani Amegan Missinou, Julie Ferreira de Carvalho, Nathalie Marnet, Thomas Delhaye, Oumayma Hamzaoui, David Abdel Sayed, Yann Guitton, Lionel Lebreton, Christophe Langrume, Anne Laperche, Regine Delourme, Maria J. Manzanares-Dauleux, Alain Bouchereau, Antoine Gravot
Summary: This study investigates the chemical diversity in 281 accessions of Brassica napus and identifies numerous rarely reported phenolics and glucosinolates. The study also reveals significant variations in the content of certain compounds among different accessions and identifies two main chemotypes associated with specific compound contents. The findings of this study provide a valuable resource for understanding the phytochemical diversity in B. napus.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Medicine, General & Internal
Luc Colas, Anne-Lise Royer, Justine Massias, Axel Raux, Melanie Chesneau, Clarisse Kerleau, Pierrick Guerif, Magali Giral, Yann Guitton, Sophie Brouard
Summary: This is an open access article published by Elsevier B.V., under the copyright of the authors and licensed under CC BY-NC-ND.
Article
Environmental Sciences
Charlotte Corporeau, Sebastien Petton, Romain Vilaca, Lizenn Delisle, Claudie Quere, Valerian Le Roy, Christine Dubreuil, Sandra Lacas-Gervais, Yann Guitton, Sebastien Artigaud, Benoit Bernay, Vianney Pichereau, Arnaud Huvet, Bruno Petton, Fabrice Pernet, Elodie Fleury, Stephanie Madec, Christophe Brigaudeau, Catherine Brenner, Nathalie M. Mazure
Summary: This study compared intertidal oysters with subtidal oysters and found that intertidal oysters at different depths delayed the onset of mortality associated with POMS. Despite slower growth, intertidal oysters showed increased resistance through metabolic, inflammatory, and immune responses, improving their overall health.
MARINE ENVIRONMENTAL RESEARCH
(2022)
Article
Agronomy
Lethicia Magno Massuia de Almeida, Erwan Corlouer, Anne Laperche, Nathalie Nesi, Alain Mollier, Sophie Brunel-Muguet
Summary: This study aims to improve predictions of crop performance under repeated high temperature events by analyzing ecoclimatic indicators. The results highlight the importance of timing, frequency, and intensity of high temperature events in relation to crop yield. Specific combinations of ecoclimatic indicators are more predictive of crop performance than a single cumulative indicator.
EUROPEAN JOURNAL OF AGRONOMY
(2022)
Article
Entomology
Diane Espel, Camille Coux, Luis R. Pertierra, Pauline Eymar-Dauphin, Jonas J. Lembrechts, David Renault
Summary: Phenotypic plasticity can result in the emergence of different morphotypes adapted to specific environmental conditions. The carabid beetle, Amblystogenium pacificum, exhibits two distinctive morphotypes based on body coloration. This study investigated how these morphotypes, as well as altitude and sexual dimorphism, were related to morphological and biochemical traits in A. pacificum. The results showed that body size and biochemical reserves differed with altitude and sex, with females having higher protein and sugar reserves. However, body size was found to be the main driver of niche partitioning along the altitudinal gradient, rather than morphotype or sex.
Article
Medicine, Research & Experimental
Thibault Leger, Aurelien Brun, Kassandra Lanchais, Jean-Paul Rigaudiere, Arnaud Briat, Yann Guitton, Fabien Marchand, Anne Tournadre, Frederic Capel
Summary: This study found that nutritional supplementation with omega 3 fatty acids can alleviate symptoms of rheumatoid arthritis and be an effective preventive treatment for patients who do not need pharmacological therapy.
