Article
Chemistry, Multidisciplinary
Elahe Memari, Fiona Hui, Hossein Yusefi, Brandon Helfield
Summary: This study aimed to investigate the effect of fluid flow rate and repeated short-pulse ultrasound on microbubble-mediated endothelial cell permeability. The results demonstrate that fast-flowing microbubbles significantly increased cell membrane permeability, and multiple short pulses of ultrasound also elevated endothelial cell permeability.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Martynas Maciulevicius, Diana Navickaite, Sonam Chopra, Baltramiejus Jakstys, Saulius Satkauskas
Summary: Research has shown that different calcium concentrations affect cell membrane permeability and cell viability during sonoporation, with ultrasound-microbubble cavitation playing a significant role. The study also indicates that inertial cavitation activities are directly involved in calcium delivery via sonoporation, leading to rapid cell death.
Review
Chemistry, Multidisciplinary
Zihao Wen, Chen Liu, Zihao Teng, Quanyi Jin, Zhihuan Liao, Xuan Zhu, Shuaidong Huo
Summary: Ultrasound has been used to enhance drug delivery efficiency by improving cell membrane permeability. The cavitation effect generated by ultrasound can facilitate the delivery of various cargoes, especially nanocarriers. Understanding the biophysical mechanism of ultrasound-cell membrane interaction is significant for the scientific community in drug delivery and nanomedicine.
Article
Chemistry, Multidisciplinary
Ines Beekers, Simone A. G. Langeveld, Bram Meijlink, Antonius F. W. van der Steen, Nico de Jong, Martin D. Verweij, Klazina Kooiman
Summary: This study investigated the interaction between microbubbles and endothelial cells during drug delivery. It found that targeted microbubbles were internalized by the cells, leading to changes in microbubble oscillation and resulting membrane perforation. The study provides important insights into the mechanisms of microbubble-mediated drug delivery.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Acoustics
Spiros Kotopoulis, Christina Lam, Ragnhild Haugse, Sofie Snipstad, Elisa Murvold, Taeraneh Jouleh, Sigrid Berg, Rune Hansen, Mihaela Popa, Emmet Mc Cormack, Odd Helge Gilja, Albert Poortinga
Summary: The aim of this study was to develop high load-capacity antibubbles that can be visualized using diagnostic ultrasound and the encapsulated drug can be released and delivered using clinically translatable ultrasound. The antibubbles were developed by optimising a silica nanoparticle stabilised double emulsion template. The results showed that the developed antibubbles can be visualized using diagnostic ultrasound and have a long half-life, indicating their potential as a viable concept for ultrasound guided drug delivery.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Sofie Snipstad, Sigurd Hanstad, Astrid Bjorkoy, Yrr Morch, Catharina de Lange Davies
Summary: Therapeutic agents benefit from encapsulation in nanoparticles for improved pharmacokinetics and biodistribution, while microbubbles in combination with ultrasound enhance delivery of nanoparticles to cells by mechanisms such as sonoporation and sonoprinting. The proximity between cells, nanoparticles, and microbubbles plays a crucial role in uptake efficiency.
Article
Multidisciplinary Sciences
Anshuman Dasgupta, Tao Sun, Roberto Palomba, Elena Rama, Yongzhi Zhang, Chanikarn Power, Diana Moeckel, Mengjiao Liu, Apoorva Sarode, Marek Weiler, Alessandro Motta, Celine Porte, Zuzanna Magnuska, Asmaa Said Elshafei, Roman Barmin, Adam Graham, Arthur McClelland, Dirk Rommel, Elmar Stickeler, Fabian Kiessling, Roger M. Pallares, Laura De Laporte, Paolo Decuzzi, Nathan McDannold, Samir Mitragotri, Twan Lammers
Summary: Surface tension allows microbubbles (MB) to have a perfect spherical shape. By stretching spherical poly(butyl cyanoacrylate) MB above their glass transition temperature, anisotropic MB can be generated, giving them unique features for biomedical applications. Compared to spherical MB, nonspherical polymeric MB exhibited superior performance including increased margination behavior, reduced macrophage uptake, prolonged circulation time, and enhanced blood-brain barrier permeation when combined with transcranial focused ultrasound (FUS). The study identifies shape as a design parameter in the MB landscape and provides a rational and robust framework for exploring the application of anisotropic MB in ultrasound-enhanced drug delivery and imaging applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Acoustics
Sofie Snipstad, Yrr Morch, Einar Sulheim, Andreas Aslund, Andre Pedersen, Catharina de Lange Davies, Rune Hansen, Sigrid Berg
Summary: Combining microbubbles with ultrasound improves drug delivery to tumors, reducing tumor growth and extending survival in experimental models. Enhanced nanoparticle uptake following ultrasound treatment underscores the need for further understanding of tumor biology and ultrasound-mediated treatment mechanisms.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Arvin Honari, Shashank R. Sirsi
Summary: Despite recent advancements in ultrasound-mediated drug delivery, no platform utilizing ultrasound contrast agents has received FDA approval. The sonoporation effect has promising applications in clinical settings, but there are safety concerns. Clinical trials are being conducted to evaluate sonoporation's efficacy in treating tumors.
Review
Pharmacology & Pharmacy
Rachel Chapla, Katherine T. Huynh, Carolyn E. Schutt
Summary: Targeted delivery of therapeutics is crucial for reducing systemic toxicity and optimizing therapeutic efficacy. Ultrasound-controlled targeting is an externally-stimulated drug delivery strategy, using ultrasound waves to stimulate gas-filled microbubbles and deliver attached cargo to surrounding cells. The attachment of nanocarrier particles to microbubbles greatly enhances drug delivery capabilities.
Article
Pharmacology & Pharmacy
Josanne S. de Maar, Charis Rousou, Benjamin van Elburg, Hendrik J. Vos, Guillaume P. R. Lajoinie, Clemens Bos, Chrit T. W. Moonen, Roel Deckers
Summary: Combining ultrasound and microbubbles (USMB) has been shown to improve drug delivery and enhance drug efficacy, but the variety of US setups and parameters used in studies hampers clinical translation.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Spiros Kotopoulis, Mihaela Popa, Mireia Mayoral Safont, Elisa Murvold, Ragnhild Haugse, Anika Langer, Georg Dimcevski, Christina Lam, Tormod Bjanes, Odd Helge Gilja, Emmet Mc Cormack
Summary: The combination of ultrasound and microbubbles has shown great promise in cancer therapy. Through the characterization of three microbubble formulations, SonoVue(R) was found to be the most effective microbubble under specific ultrasound conditions. This was validated in vivo with improved survival and tumor growth inhibition. In vitro, the best microbubble formulation depends on the ultrasound parameters and concentration used.
Article
Pharmacology & Pharmacy
Yongho Jang, Jeehun Park, Pilsu Kim, Eun-Joo Park, Hyungjin Sun, Yujin Baek, Jaehun Jung, Tai-kyong Song, Junsang Doh, Hyuncheol Kim
Summary: In this study, exosome-fused microbubbles (Exo-MBs) were developed by embedding the exosome membrane proteins into microbubbles. The Exo-MBs showed improved stability over conventional microbubbles, and the resulting Exo-NPs demonstrated favorable targeting properties to their original cells. Loading a photosensitizer into Exo-MBs enhanced the therapeutic efficacy of photodynamic therapy, followed by cancer immunotherapy.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Pharmacology & Pharmacy
Oystein Eikrem, Spiros Kotopoulis, Mihaela Popa, Mireia Mayoral Safont, Kjell Ove Fossan, Sabine Leh, Lea Landolt, Janka Babickova, Oddrun Anita Gudbrandsen, Odd Helge Gilja, Bettina Riedel, Jan Schjott, Emmet McCormack, Hans-Peter Marti
Summary: The use of ultrasound and microbubble-enhanced drug delivery, known as sonoporation, has shown promising results in numerous clinical trials. However, further studies are needed to assess the impact on healthy tissues. This study focused on testing the feasibility of low-intensity sonoporation on healthy kidneys and found that it has the potential to enhance drug uptake without causing long-standing damage to the kidneys.
Article
Chemistry, Multidisciplinary
Benjamin van Elburg, Joke Deprez, Martin van den Broek, Stefaan C. De Smedt, Michel Versluis, Guillaume Lajoinie, Ine Lentacker, Tim Segers
Summary: Sonoporation is a process facilitated by ultrasound-driven microbubble oscillations that enhances intracellular drug delivery. This study aimed to investigate the role of microbubble size on sonoporation and the relevant mechanism(s). The results showed that sonoporation efficiency strongly depended on the equilibrium bubble size, with bubbles of approximately 4.7 μm radius being the most efficient. Shear stress was unlikely to be the mechanism of action, while normal stress correlated well with sonoporation efficiency.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Biotechnology & Applied Microbiology
Gareth LuTheryn, Peter Glynne-Jones, Jeremy S. Webb, Dario Carugo
MICROBIAL BIOTECHNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Valerio Pereno, Junjun Lei, Dario Carugo, Eleanor Stride
Article
Chemistry, Analytical
Antonio De Grazia, Gareth LuTheryn, Alireza Meghdadi, Ali Mosayyebi, Erika J. Espinosa-Ortiz, Robin Gerlach, Dario Carugo
Review
Engineering, Biomedical
Alireza Meghdadi, Stephen A. Jones, Venisha A. Patel, Andrew L. Lewis, Timothy M. Millar, Dario Carugo
Summary: This review article focuses on the importance and development trends of foam sclerotherapy in the treatment of varicose veins. Despite the advantages of foam therapy, high recurrence rates post-treatment indicate the need for more effective and long-lasting treatment methods.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2021)
Article
Pharmacology & Pharmacy
Fatih Yanar, Ali Mosayyebi, Claudio Nastruzzi, Dario Carugo, Xunli Zhang
Article
Acoustics
Sara Ferri, Qiang Wu, Antonio De Grazia, Anastasia Polydorou, Jonathan P. May, Eleanor Stride, Nicholas D. Evans, Dario Carugo
Summary: The study showed that during the preparation of NDs, factors such as PFP concentration, sonication power and duration, and the addition of a lipophilic fluorescent dye can affect the size and distribution of NDs. NDs exhibited different stability under various conditions, and phase transition may occur at 37℃.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Acoustics
Qiang Wu, Christophoros Mannaris, Jonathan P. May, Luca Bau, Anastasia Polydorou, Sara Ferri, Dario Carugo, Nicholas D. Evans, Eleanor Stride
Summary: The vaporization process of phospholipid-coated perfluorobutane nanodroplets (PFB NDs) was studied using ultrahigh-speed optical imaging, B-mode ultrasound, and passive cavitation detection. The results indicate that PFB NDs can be vaporized at moderate peak negative pressures, pulse lengths, and pulse repetition frequencies, making them potential cavitation agents. Application-specific thresholds should be defined based on the desired biological/therapeutic effect.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2021)
Article
Engineering, Biomedical
Ali Mosayyebi, Aravinthan Vijayakumar, Maryam Mosayebi, Dirk Lange, Bhaskar K. Somani, Costantino Manes, Dario Carugo
Summary: This study investigated the relationship between flow dynamics and particle accumulation in ureteric stents through computational and experimental methods. The results indicated an inverse correlation between wall shear stress and crystal accumulation at side-holes, with higher wall shear stress levels leading to reduced accumulation. Low wall shear stress levels in side-holes were found to be prone to particle accumulation. The findings highlight the importance of wall shear stress in modulating the localization and extent of particle accumulation in ureteric stents.
APL BIOENGINEERING
(2022)
Article
Computer Science, Interdisciplinary Applications
Xiaohan Yang, Ali Mosayyebi, Dario Carugo
Summary: In this study, a CFD model of the urinary tract was developed to investigate the flow dynamics with a porous ureteric stent. The results showed that increasing the permeability of the porous material could enhance the mass flow rate and fluid velocity within the stent. Moreover, the porous stent demonstrated better drainage performance compared to the unporous stent, especially in the presence of ureteral occlusion.
MEDICAL & BIOLOGICAL ENGINEERING & COMPUTING
(2022)
Article
Acoustics
F. I. L. I. P. Plazonic, G. A. R. E. T. H. LuTheryn, C. H. A. R. L. O. T. T. E. Hind, M. E. L. A. N. I. E. Clifford, M. I. C. H. A. E. L. Gray, E. L. E. A. N. O. R. Stride, P. E. T. E. R. Glynne-Jones, M. A. R. T. Y. N. Hill, J. Mark Sutton, D. A. R. I. O. Carugo
Summary: The aim of this research was to explore the interaction between ultrasound-activated microbubbles (MBs) and Pseudomonas aeruginosa biofilms. The study found that the bactericidal efficacy of MBs and ultrasound varied in different tissue environments due to different treatment parameters.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2022)
Review
Microbiology
Victor Choi, Jennifer L. Rohn, Paul Stoodley, Dario Carugo, Eleanor Stride
Summary: In this Review, emerging drug delivery strategies are discussed as a means to improve the clinical efficacy of antibiofilm agents in preventing and eliminating pathogenic biofilms. The potential benefits of these techniques include improved penetration of antimicrobials through biofilms, enhanced drug dispersal, and synergistic bactericidal effects. However, current limitations and future prospects for these therapies in the clinic are also highlighted.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Gareth LuTheryn, Elaine M. L. Ho, Victor Choi, Dario Carugo
Summary: The presence of multi-drug resistant biofilms in chronic, persistent infections is a major obstacle for successful clinical outcomes. This research aims to develop cationic gas-filled microbubbles that can non-selectively target negatively charged biofilms to enhance drug delivery. Compared to uncharged microbubbles, cationic microbubbles showed a significant increase in their ability to bind and sustain interaction with biofilms. This study demonstrates the utility of charged microbubbles for non-selective targeting of bacterial biofilms and could greatly improve stimuli-mediated drug delivery.
Review
Biochemistry & Molecular Biology
Fatih Yanar, Dario Carugo, Xunli Zhang
Summary: Organic and inorganic nanoparticles have been extensively studied for their unique properties and potential applications in various fields. Recently, hybrid nanomaterials combining organic nanocompartments with inorganic nanoparticles have attracted attention for drug delivery and theranostic applications. These hybrid systems offer improved stability, bioavailability, and reduced toxicity, as well as synergistic interactions between organic and inorganic nanoparticles. Moreover, they serve as multifunctional agents for advanced bioimaging and targeted drug delivery, with great potential in clinical applications.
Review
Urology & Nephrology
Meghana Ramachandra, Ali Mosayyebi, Dario Carugo, Bhaskar K. Somani
RESEARCH AND REPORTS IN UROLOGY
(2020)
Article
Biochemistry & Molecular Biology
Erdinc Sezgin, Dario Carugo, Ilya Levental, Eleanor Stride, Christian Eggeling
Review
Chemistry, Multidisciplinary
Zhiyuan Shi, Yong Hu, Xin Li
Summary: Controlled drug delivery systems that can respond to mechanical force offer a unique solution for on-demand drug activation and release. Among various mechanical stimuli, ultrasound (US) has advantages in achieving spatiotemporally controlled drug release. Traditional US-triggered drug release relies on heat-induced phase transitions or chemical transformations, while the cutting-edge approach of Sonopharmacology leverages polymer mechanochemistry. The remaining challenges and potential future directions in this field are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Lijie Zheng, Yuanzheng Chen, Xun Gu, Yingying Li, Hanqing Zhao, Wenjun Shao, Tao Ma, Chuanbin Wu, Qingqing Wang
Summary: In this study, a novel dosage form consisting of dissolving microneedles and an adhesive transdermal patch was developed for the treatment of rheumatoid arthritis. In vitro and in vivo experiments demonstrated that the combination of drugs delivered by this dosage form effectively reduced joint inflammation and damage.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Kyungjik Yang, Hwa Seung Han, Seung Hwan An, Kyung Hoon Park, Keonwook Nam, Shinha Hwang, Yuyeon Lee, Sung Yeon Cho, Taehyung Kim, Deokyeong Choe, Sang Won Kim, Wonkyu Yu, Hyunah Lee, Jiyong Park, Sangguan You, Dong- Gyu Jo, Ki Young Choi, Young Hoon Roh, Jae Hyung Park
Summary: This study developed CP-loaded CS microcapsules to enhance the oral bioavailability of CP through controlled gastrointestinal delivery. The optimized microcapsules exhibited desirable physicochemical properties, showed anti-photoaging effects via antioxidant activity, and achieved controlled release in the gastrointestinal tract. This research provides a simple and economical approach for enhancing the oral bioavailability of CP for customized bioactive compound administration.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Huiyang Li, Shuo Liu, Wenjin Dai, Bingmei Yao, Yong Zhou, Sujia Si, Hairong Yu, Riguang Zhao, Fang Jin, Liqun Jiang
Summary: Changes in bodily fluid pressures are crucial in diseases like high-altitude pulmonary edema (HAPE). Researchers have developed hydrostatic pressure-sensitive multivesicular liposomes (PSMVLs) that can release drugs in response to pressure changes, with potential applications in HAPE treatment. Animal experiments showed that this system provides better protection for lung tissues and respiratory function, reducing the occurrence of pulmonary edema.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Qian Hu, Hongbing Lan, Yinmei Tian, Xiaonan Li, Mengmeng Wang, Jiao Zhang, Yulin Yu, Wei Chen, Li Kong, Yuanyuan Guo, Zhiping Zhang
Summary: Coacervate droplets formed through liquid-liquid phase separation have potential as delivery vesicles for therapeutics. However, their lack of physiological stability and membranes are challenges. In this study, polylysine-polynucleotide complex coacervate droplets with favorable stability were formulated to concentrate molecules and nanoparticles. Phospholipid membranes were further coated on the droplets to create coacervate-based artificial protocells (ArtPC) with membrane-like structures. These biofunctional ArtPC effectively reduced blood uric acid levels and prevented renal injuries.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Xiaowei Wang, Hongwei Lu, Fang Luo, Dan Wang, Apeng Wang, Xuelei Wang, Wenkai Feng, Xiaobo Wang, Jiayi Su, Mingliang Liu, Guimin Xia
Summary: Four novel lipid-like GEM diesters were synthesized and encapsulated into liposomes to improve the antitumor efficacy of Gemcitabine. The liposomes loaded with dimyristoyl GEM (LipodmGEM) showed enhanced cellular uptake, improved inhibition of cell migration, and a greatly extended half-life compared to free Gemcitabine. LipodmGEM successfully enriched the drug in the tumor and exhibited excellent anticancer efficacy in vivo with negligible systemic toxicity.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Yajing Sun, Ze Lu, John A. Taylor, Jessie L. S. Au
Summary: A recent development in cancer chemotherapy is the use of cytotoxics to induce tumor-specific immune response through immunogenic cell death (ICD). This study describes a method that utilizes immunostaining and machine-learning to identify cells with ecto-CRT in intact 3-dimensional tissues. The method was successfully applied to study drug-induced ICD in human bladder cancer.
JOURNAL OF CONTROLLED RELEASE
(2024)
Review
Chemistry, Multidisciplinary
Rafat Ali, Shantanu Sen, Rohil Hameed, Aamir Nazir, Sandeep Verma
Summary: This review provides a focused overview of emerging strategies for delivering gasotransmitters in a controlled and sustained manner to reestablish neurophysiological homeostasis.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Jing Chen, Xinyi Zhang, Jinshen Zhang, Zhaoxia Wang, Guilan Zhu, Ming Geng, Jinmiao Zhu, Yajun Chen, Wei Wang, Youcui Xu
Summary: In this study, a multifunctional responsive hydrogel system was developed for synergistic reoxygenation and chemo/photothermal therapy. The hydrogel system showed both therapeutic effects against metastatic breast cancer and wound infection, making it a promising strategy for treating and preventing tumor recurrence and associated wound infection.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Baoshan Huang, Na Zhang, Xinying Qiu, Rui Zeng, Shuimiao Wang, Mengxia Hua, Qing Li, Kaihui Nan, Sen Lin
Summary: This study revealed that robust ROS can oxidize mitochondrial DNA (ox-mtDNA) and cause its release into the cytosol, resulting in the activation of NLRP3 inflammasome. By using the mitochondria-targeted antioxidant SkQ1 and a novel mitochondria-targeted nanoparticle (SkQ1 NP), it was found that mitochondrial ROS scavenging could in situ inhibit DED-induced mtDNA oxidation and suppress NLRP3-mediated inflammation.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Wenqi Liu, Cheng Hu, Linyu Long, Shuyi He, Wen Zhang, Zhicun Wang, Li Yang, Yunbing Wang
Summary: Myocardial infarction is the leading cause of cardiovascular mortality, and current treatment methods have limitations. This study developed a smart carrier that can release different therapeutic substances for different pathological processes, effectively improving cardiac function, promoting cardiac repair, and preventing ventricular remodeling.
JOURNAL OF CONTROLLED RELEASE
(2024)