Article
Virology
Huimin Bai, Michiyo Kataoka, Yasushi Ami, Yuriko Suzaki, Naokazu Takeda, Masamichi Muramatsu, Tian-Cheng Li
Summary: Rabbit hepatitis E virus (HEV) is a novel HEV which belongs to genotype 3 in the Orthohepevirus A species. Through expressing truncated rabbit HEV ORF2 proteins, two types of virus-like particles were obtained, with RsVLPs showing stronger antigenic cross-reactivity against different HEV genotypes. Immunization with RsVLPs completely protected rabbits from challenge by rabbit HEV, suggesting potential for vaccine development.
Article
Biochemical Research Methods
Ryan Dickerson, Christopher Argento, John Pieracci, Meisam Bakhshayeshi
Summary: This study presents a novel anion exchange chromatography process for enriching full rAAV2 particles, with an identified operating design space to ensure process robustness. Isocratic chromatography offers advantages over traditional shallow linear gradient elution, including lower buffer consumption, smaller intermediate pool volumes, and more robust manufacturing.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Veterinary Sciences
Alena Vrablikova, Veronika Brezani, Ivan Psikal, Martin Fraiberk, Marek Sebela, Martina Fojtikova, Pavel Kulich, Renata Hezova, Josef Masek
Summary: Bovine papillomavirus type 1 L1 protein was expressed, purified, and confirmed as virus-like particles (VLPs) through electron microscopy and mass spectrometry analysis. Mice immunized with purified VLPs showed an increased humoral immune response, with the best response observed in the group immunized with the highest dose of antigen. These VLPs purified by affinity chromatography using lectins have potential applications for prophylactic immunization in veterinary medicine.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Maryam Moazami Goodarzi, Reza Jalalirad, Delaram Doroud, Hamidreza Hozouri, Mohammadreza Aghasadeghi, Mahdi Paryan
Summary: In this study, the purification of VLP-based recombinant hepatitis B surface antigen (rHBsAg) was optimized by selecting buffering conditions. The new purification method showed improved protein purity and can be a substitute for the conventional method.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Gergana Zahmanova, Milena Mazalovska, Katerina Takova, Valentina Toneva, Ivan Minkov, Hadrien Peyret, George Lomonossoff
Summary: This study demonstrates that HBcAg is a promising carrier platform for neutralizing epitopes of hepatitis E virus (HEV), and the chimeric HBcAg/HEV VLPs could be a candidate for a bivalent vaccine.
Article
Engineering, Chemical
A. A. M. Abusultan, J. A. Wood, T. Sainio, A. J. B. Kemperman, W. G. J. van der Meer
Summary: Reverse osmosis (RO) membrane technology is increasingly used for large-scale production of potable water. However, the acidic and unbuffered RO permeate can damage water piping networks. Remineralization using an ion exchange resin - bipolar membrane electrodialysis hybrid process can protect the water distribution network. This study investigates the use of preparative ion exchange chromatography principles for monovalent-divalent ion separation.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Ayaka Washizaki, Asako Murayama, Megumi Murata, Tomoko Kiyohara, Keigo Yato, Norie Yamada, Hussein Hassan Aly, Tomohisa Tanaka, Kohji Moriishi, Hironori Nishitsuji, Kunitada Shimotohno, Yasumasa Goh, Ken J. Ishii, Hiroshi Yotsuyanagi, Masamichi Muramatsu, Koji Ishii, Yoshimasa Takahashi, Ryosuke Suzuki, Hirofumi Akari, Takanobu Kato
Summary: The study found that an HB vaccine consisting of large-HBsAg can overcome the shortcomings of the current HB vaccine. The large-HBsAg vaccine induces antibodies that can neutralize hepatitis B virus (HBV) infections with vaccine-escape mutations (VEMs).
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Chemical
Tomas Molnar, Maria Bartosova, Monika Antosova, Ludovit Skultety, Milan Polakovic
Summary: This study successfully purified rhEPO using a three-step chromatographic procedure, with a purification factor of about 40. The process involved direct chromatographic separation of the protein from the post-harvest medium, with the removal of over 99% of impurity proteins.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Yue Sun, Chengyun Liao, Feifang Zhang, Zhenzi Xu, Bingcheng Yang
Summary: A gas-free continuously regenerated-anion impurity removal device (CR-ARD) has been developed for ion chromatography (IC), which enables continuous regeneration of the anion exchange resin without the need for offline regeneration, effectively removing non-hydroxide anionic impurities.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Jie Yu, Jo-Shu Chang, Hongliang Guo, Song Han, Duu-Jong Lee
Summary: Sulfuric acid treatment modifies the biochar derived from corn cobs, stalks, and reeds. The modified biochars, especially the corn cobs-biochar, have significantly higher BET values and superior Na+ adsorption capacity compared to the pristine biochars and literature reports. The presence of -SO3H groups on the biochar surface contributes to its enhanced Na+ adsorption capacity, indicating potential application for sodium-contaminated water remediation.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Michal Halaj, Maria Matulova, Peter Capek
Summary: Ion-exchange chromatography was used to separate the biologically active extracellular biopolymer produced by D. chlorelloides into ten fractions. These fractions differed in terms of yield, protein content, monosaccharide composition, and molecular weight distribution. The dominant component in all fractions, except one, was rhamnogalactans, which were substituted to different extents by mannose and glucose.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Environmental
Karl Zimmermann, Jaycee Wright, Pierre Berube, Benoit Barbeau, Madjid Mohseni
Summary: This study confirms the effectiveness and feasibility of Biological Ion Exchange (BIEX) technology in removing dissolved organic carbon (DOC). Results show that BIEX technology can achieve DOC removal levels ranging from 32% to 50% when treating inlet water with low or high sulphate and DOC concentrations. Additionally, the study found that in the secondary ion exchange mechanism, the removed anions directly exchange in charge equivalence with the released anions.
Article
Pharmacology & Pharmacy
Norhan S. Sheraba, Ahmed Hesham, Mohamed Fawzy, Emad Diab, Mohamad E. Basuony, Aymen S. Yassin, Hamdallah H. Zedan, Mohammed Abu-Elghait
Summary: This comprehensive literature assessment focuses on strategies for detecting and removing endotoxins from biotechnological preparations. It provides a brief history of endotoxins and discusses their chemical and physical features, as well as the pathophysiological consequences of excessive or systemic exposure to LPS. The study explores various methods for endotoxin detection and removal, including known approaches and cutting-edge technology, with affinity resin-based chromatography identified as the most efficient method.
Article
Environmental Sciences
Zhen Liu, Morgan Solliec, Isabelle Papineau, Kim M. Lompe, Madjid Mohseni, Pierre R. Berube, Sebastien Sauve, Benoit Barbeau
Summary: This study confirms the contribution of biodegradation on biological ion exchange (BIEX) resins in removing organic micropollutants, while noting that the absence of an adapted bacterial community may lead to lower biodegradation rates. Additionally, nonbiodegradable and neutral micropollutants showed minimal removal during batch tests on BIEX resins.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Xuyang Cao, Min Huang, Ying Wang, Yanzhi Chen, Hanwen Yang, Fusheng Quan
Summary: In this study, the recombinant Cap protein of porcine circovirus type 3 was successfully expressed in Escherichia coli by deleting the nuclear localization sequence (NLS), and the VLPs were observed by transmission electron microscopy. The immunogenicity of the recombinant Cap protein was evaluated in mice and it induced higher levels of humoral and cellular immune responses. A VLP-based ELISA method was developed for the detection of antibodies, which showed good sensitivity, specificity, repeatability, and clinical applicability. These results have significant implications for the prevention, diagnosis, and control of porcine circovirus type 3 associated diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Mahshid Yousefipour, Maryam Khatami, Amin Javidanbardan, Seyed Nezamedin Hosseini, Mohammadreza Mehrnia
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2019)
Article
Biochemical Research Methods
Mehri Mortazavi, Mohammad Ali Shokrgozar, Soroush Sardari, Kayhan Azadmanesh, Reza Mahdian, Hooman Kaghazian, Seyed Nezamedin Hosseini, Mohammad Hossein Hedayati
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2019)
Article
Biochemical Research Methods
Roya Khosravi, Seyed Nezamedin Hosseini, Amin Javidanbardan, Maryam Khatami, Hooman Kaghazian, Seyed Dawood Mousavi Nasab
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Seyed Nezamedin Hosseini, Amin Javidanbardan, Maryam Khatami
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2019)
Article
Biotechnology & Applied Microbiology
Alireza Rahimi, Seyed Nezamedin Hosseini, Amin Jauidanbardan, Maryam Khatami
FOOD AND BIOPRODUCTS PROCESSING
(2019)
Article
Biotechnology & Applied Microbiology
Ahmad Beiroti, Seyed Nezamedin Hosseini, Mohammad Reza Aghasadeghi, Dariush Norouzian
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2019)
Article
Environmental Sciences
L. Mahmoudian-Boroujerd, S. N. Hosseini, A. Karimi-Jashni, M. Khatami, S. Javadpour
Summary: This research aimed to optimize and model the synthesis and application of a novel visible-light-driven Ag/C-TiO(2) nanophotocatalyst for degradation of recombinant DNA in Hepatitis B surface antigen production plant wastewater. The synthesized nanoparticles were characterized and evaluated experimentally to achieve a 60% degradation of recombinant DNA at the optimized operation conditions. The statistical analysis highlighted the significant effects of carbon dopant content, calcination temperature, initial recombinant DNA concentration, and contact time on the degradation process.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Reza Mahdavi, S. Siamak Ashraf Talesh
Summary: The degradation kinetics of methyl orange on Al/Ca co-doped ZnO photocatalysts was investigated in this research. The role of Ca co-dopant in enhancing the photocatalytic activity of Al-doped ZnO was analysed using the obtained kinetic parameters. The Langmuir-Hinshelwood kinetics model was found to fit the reaction well. Characterization techniques such as XRD, SEM, TEM, and UV-Vis spectroscopy were used to study the samples. The results showed that Ca and Al occupied Zn sites without altering the wurtzite structure of ZnO nanoparticles. Increasing Ca co-doping led to rod-like morphology in Al-doped ZnO nanoparticles. The UV-Vis absorption spectra revealed a red-shift of the band-edge in Al/Ca co-doped ZnO nanoparticles compared to pure ZnO, indicating a decrease in optical band gap and an increase in absorption in the visible region. Compared to Al-doped ZnO nanoparticles, Al/Ca co-doped ZnO nanoparticles exhibited higher photocatalytic activity. The optimal Al and Ca content for achieving the highest photocatalytic efficiency were found to be 5% Al and 3% Ca doping (Zn0.92Al0.05Ca0.03O).
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Reza Mahdavi, S. Siamak Ashraf Talesh
Summary: This research investigates the self-cleaning property of Al-doped ZnO nanoparticles synthesized via the sonochemical method. The nanoparticles are used as an additive in waterborne acrylic resin-based coatings to study their photocatalytic and hydrophilic activities. Through XRD, TEM, EDS, and UV-Vis analysis, the influence of different doping amounts of Al on the crystallographic structures, morphologies, and optical properties of the nanoparticles is evaluated. The results show that the morphology of the nanoparticles changes with different doping amounts, and the doped nanoparticles in the coating exhibit higher degradation and lower contact angle.
JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Amin Javidanbardan, Ana M. Azevedo, Virginia Chu, Joao P. Conde
Summary: This study explores the potential of micro- and mesoscale milling technologies for developing cost-effective microfluidic systems. The results demonstrate that the surface roughness can be reduced by implementing milling optimization and postprocessing steps. Additionally, the use of a specifically designed negative mold for replication allows for the rapid development of microfluidic structures with high surface quality and structural integrability.
Article
Biochemical Research Methods
Laleh Abdollahzadeh, Masoumeh Seyfi Mazraeno, Seyed Nezamedin Hosseini, Alireza Fazlali, Maryam Khatami
Summary: Microfluidic particle sorter (miniaturized hydrocyclone) has been proposed as a solution to reduce the number of separation steps and the risk of contamination in various industries. This research investigates the separation of biomass from fermentation broth using 3 mm hydrocyclones. The most efficient mini hydrocyclone was chosen and studied experimentally, achieving a separation efficiency of up to 90%.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Alireza Kavianpour, Mohsen Ashjari, Seyed Nezamedin Hosseini, Maryam Khatami
Summary: This study quantified the interaction between lipopolysaccharide (LPS) and recombinant hepatitis B surface antigen (r-HBsAg) using immunoaffinity chromatography (IAC) and revealed the results through dynamic light scattering and transmission electron microscopy. The findings showed an inverse relationship between LPS concentration and interaction with HBsAg, providing a practical method for quantifying the interaction between LPS and target recombinant proteins.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2023)