Article
Materials Science, Paper & Wood
Li Huang, Wei Yuan, Yue Hong, Suna Fan, Xiang Yao, Tao Ren, Lujie Song, Gesheng Yang, Yaopeng Zhang
Summary: A novel biomaterial ink composed of regenerated silk fibroin and TEMPO-oxidized bacterial cellulose nanofibrils has been developed for 3D printing lung tissue scaffolds. The addition of OBC nanofibrils increased ink viscosity and improved scaffold shape fidelity, with optimal printing parameters identified as 0.3 bar printing pressure, 45 mm/s printing speed, and 410 μm nozzle diameter. OBC nanofibrils were aligned along print lines, guiding the orientation of lung epithelial stem cells and maintaining their proliferation ability and epithelial phenotype after 7 days of culture.
Article
Materials Science, Multidisciplinary
Kai Yan, Xiao Zhang, Yang Liu, Jiangqi Cheng, Chenjun Zhai, Kai Shen, Wenwei Liang, Weimin Fan
Summary: In this study, a porous SF-HPCMA bioink capable of 3D bioprinting was prepared for the first time using silk fibroin (SF) and hydroxypropyl cellulose methacrylate (HPCMA). The SF-HPCMA scaffolds bioprinted using this bioink showed excellent mechanical properties and suitable porosity. In vitro experiments demonstrated that the SF-HPCMA scaffolds were highly biocompatible and could effectively promote cell proliferation and chondrogenic differentiation, resulting in higher quality cartilage tissue. Implantation of SF-HPCMA scaffolds successfully repaired articular cartilage defects in rabbit knees.
MATERIALS & DESIGN
(2023)
Article
Agricultural Engineering
Xiran Qiao, Kuanjun Fang, Xiuming Liu, Jixian Gong, Shuai Zhang, Jinkun Wang, Min Zhang, Fuyun Sun
Summary: In this study, hydroxypropyl methyl cellulose (HPMC) was used to create a continuous film on the surface of cotton/polyamide interwoven fabric, addressing the challenges posed by acid-base conditions, high temperature, and high humidity in inkjet printing on multi-component fabrics. HPMC connected the fibers in the yarn, preventing ink penetration, and maintained high viscosity under acidic conditions, allowing for dyeing of polyamide fibers with reactive dyes. Compared to sodium alginate, HPMC significantly improved the color depth of cyan, magenta, yellow, and black on cotton/polyamide fabrics.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Polymer Science
Chavee Laomeephol, Apichai Vasuratna, Juthamas Ratanavaraporn, Sorada Kanokpanont, Jittima Amie Luckanagul, Martin Humenik, Thomas Scheibel, Siriporn Damrongsakkul
Summary: Binary-blended hydrogels fabricated from Bombyx mori silk fibroin and recombinant spider silk protein showed improved gelation time and cellular interactions compared to individual materials, suggesting potential for biomedical applications. Mixing the two materials enhanced cellular activities, making the hydrogels suitable for various biomedical uses.
Article
Chemistry, Multidisciplinary
Ziyang Sun, Haoran Liu, Dandan Dai, Hao Lyu, Ruochuan Huang, Wenzhao Wang, Chengchen Guo
Summary: This study presents a promising injectable cell-laden hydrogel system based on silk acid, which exhibits good cytocompatibility and injectability. The hydrogel has potential applications in 3D cell culture, targeted cell delivery, and tissue engineering.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Physical
G. Poggi, D. Chelazzi, M. Laurati
Summary: The addition of cellulose nanocrystals accelerates gelation kinetics and increases the stiffness of gels formed by silk fibroin. Continuous shear tests revealed a higher yield stress in silk fibroin/cellulose nanocrystal gels, with the yield strain decreasing at higher shear rates possibly due to shear-induced compaction. Large amplitude oscillatory shear (LAOS) measurements demonstrated a broad and gradual transition from solid to fluid in the gels, with two distinct yield strains indicating different stages of fluidization.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Materials Science, Multidisciplinary
Shuying Li, Chuanzhen Huang, Hanlian Liu, Xu Han, Zhichao Wang, Jun Huang, Yonggan Yan, Zhen Wang
Summary: Cell adhesion is crucial for tissue formation. Developing a bioink with tunable mechanical properties and enhanced cell adhesion is a challenge in tissue engineering. In this study, a printable hydrogel was developed by modifying GelMA/HAMA with SilMA. The hydrogel showed good photocuring behavior and was suitable for DLP 3D bioprinting. SilMA content could tailor the mechanical and biological properties of the hydrogel. SilMA/GelMA/HAMA hydrogel exhibited higher adhesion strength and enhanced cell actin expression. The 3D printed scaffold structure remained stable and supported cell survival and proliferation. The hydrogel has broad implications for customized 3D structures in tissue engineering.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Green & Sustainable Science & Technology
Kun Zhang, Kuanjun Fang, Yawei Song, Fuyun Sun
Summary: An organic solvent-free dye ink was developed using HPMC, which showed better droplet formation and deeper color in printed patterns compared to DEG and commercial inks. The HPMC/dye ink also had lower coloring effluent, making it a clean ink with high performance and lower pollution and cost.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Materials Science, Paper & Wood
Zhuotong Wu, Yu Jiang, Zhe Li, Baoxiu Wang, Huaping Wang, Shiyan Chen
Summary: This study presents a simple strategy to adjust the distribution of bacterial cellulose nanofibers on biomimetic 3D scaffolds by selecting gelatin and silk fibroin with positive and negative charges, respectively. Different structures and properties of protein scaffolds can be obtained by adjusting the proportion of Gel and SF, leading to different densities and distributions of bacteria on the scaffolds. This approach provides a simple idea for guiding bacteria growth on tissue engineering scaffolds, which can be applied in medical implants, cell supports, and tissue regeneration.
Article
Chemistry, Applied
Xiran Qiao, Kuanjun Fang, Xiuming Liu, Jixian Gong, Shuai Zhang, Jinkun Wang, Min Zhang
Summary: In this study, hydroxypropyl methyl cellulose was used to pretreat cotton/polyamide fabric, improving the fabric's surface properties and enhancing the color strength and sharpness of inkjet printing. Compared with sodium alginate, the pretreated fabric showed significant improvement in inkjet printing performance. This research demonstrates that HPMC pretreatment is a simple and effective method with great potential for industrial production in the future.
PROGRESS IN ORGANIC COATINGS
(2022)
Article
Biochemistry & Molecular Biology
Keqin Liu, Kuanjun Fang, Weichao Chen, Chunming Zhang, Liyuan Sun, Jilin Zhu
Summary: A simple and effective coating method was proposed to improve printing quality and material utilization rate. The flow behavior of ink droplets on silk fabric surfaces treated with different chemicals was observed and measured. The results showed that Hydroxyethyl methyl cellulose (HEMC) formed the densest and smoothest film on silk fabrics, resulting in the most hydrophobic surface. Furthermore, HEMC showed a comparative advantage over Hydroxyethyl cellulose (HEC) in improving color effect and increasing dye utilization rate.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Anongnart Duangpakdee, Chavee Laomeephol, Depicha Jindatip, Peerapat Thongnuek, Juthamas Ratanavaraporn, Siriporn Damrongsakkul
Summary: The study developed 3D porous scaffolds fabricated from blends of silk fibroin, gelatin, and hyaluronan, which showed water stability and enhanced swellability. The scaffolds supported cell adhesion and proliferation, had high porosity, interconnectivity, and observed cell penetration into the structures.
Article
Materials Science, Multidisciplinary
Ying Zhou, Jiaxue Wu, Yanyi Li, Wei Zhang, Yushan Zou, Lian Duan, Xiao Yang, Bo Xiao, Shixiong Yi
Summary: In this study, silk fibroin/cellulose blend nanofibrous membranes modified with sodium-3-sulfobenzoate (S-SCBNM) were fabricated for lysozyme adsorption using electrospinning technique. The results showed that S-SCBNM exhibited properties of ultrathin fiber diameter, fast adsorption equilibrium, excellent adsorption performance, and good reversibility, making them promising for proteins separation.
ADVANCED FIBER MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Jun Li, Fei Yang, Dongyan Liu, Sensen Han, Junsheng Li, Guoxin Sui
Summary: Based on the good dispersing effect of nanocellulose, a high content graphene (50 wt%) composite paper with micro/nanocellulose fibers and silk fibroin (SF) was manufactured. The synergistic effects of cellulose microfibers (CMFs), cellulose nanofibers (CNFs), and SF led to a paper with ideal combination of flexibility, electrical conductivity, and mechanical strength. The composite paper shows a tensile strength of 49.29 MPa, surface resistance of 39.0-42.1 Omega, and good joints bend sensing performance. The graphene-based conductive paper is helpful for the development of smart clothing wearable biosensing devices.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Polymer Science
Michael G. Sun, Yuncin Luo, Tao Teng, Victor Guaiquil, Qiang Zhou, Lander McGinn, Osayd Nazzal, Michael Walsh, James Lee, Mark I. Rosenblatt
Summary: Silk fibroin films are promising biomaterials for corneal tissue engineering, offering the ability to mimic native corneal tissue, promote wound healing, and regulate cellular behavior through stiffness tunability. Studies have shown that corneal epithelial cells are mechanosensitive to different stiffnesses of silk films, suggesting that film stiffness can be used to modulate cell behavior for ocular surface repair.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2021)
Letter
Surgery
Xiaoyi Hou, Alexander C. Rokohl, Yongwei Guo, Ludwig M. Heindl
AESTHETIC PLASTIC SURGERY
(2022)
Letter
Ophthalmology
Senmao Li, Alexander C. Rokohl, Yongwei Guo, Ludwig M. Heindl
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Xiaoyi Hou, Alexander C. Rokohl, Marius M. Meinke, Ming Zhang, Yongwei Guo, Ludwig M. Heindl
Summary: This study compared the differences between 2D and 3D techniques in assessing medial canthal tendon (MCT) laxity and found a significant correlation between 3D measurements and MCT laxity, while no significant correlation was found with 2D measurements. The results suggest that measurements on 3D images can be used as predictors of MCT laxity.
JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY
(2022)
Article
Ophthalmology
Senmao Li, Yongwei Guo, Xiaoyi Hou, Jinhua Liu, Wanlin Fan, Sitong Ju, Philomena A. Wawer Matos, Alexander C. Rokohl, Ludwig M. Heindl
Summary: Research trends for uveal melanoma have shown a steady increase in the past two decades, focusing on genetics and immunology. Co-occurrence analysis revealed 6 research hotspots based on author keywords.
INTERNATIONAL OPHTHALMOLOGY
(2022)
Article
Medicine, General & Internal
Wanlin Fan, Yongwei Guo, Xiaoyi Hou, Jinhua Liu, Senmao Li, Sitong Ju, Philomena Alice Wawer Matos, Michael Simon, Alexander C. Rokohl, Ludwig M. Heindl
Summary: Portable VECTRA H2 device showed good intra-device reliability for assessing periocular region, while inter-device reliability deteriorated, suggesting that using the average of two scans could improve inter-device reliability.
FRONTIERS IN MEDICINE
(2022)
Article
Ophthalmology
Jinhua Liu, Alexander C. Rokohl, Honglei Liu, Wanlin Fan, Senmao Li, Xiaoyi Hou, Sitong Ju, Yongwei Guo, Ludwig M. Heindl
Summary: The purpose of this study was to investigate age-and sex-related changes in periocular morphology in Caucasians using a standardized protocol. The results showed significant differences in palpebral fissure width, position changes of endocanthion and exocanthion, and palpebral fissure inclination between different age and gender groups. The position change of the pupil was minimal relative to age, suggesting the need to establish a reference plane to describe periocular changes.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Letter
Oncology
Wanlin Fan, Alexander C. Rokohl, Yongwei Guo, Ludwig M. Heindl
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Ophthalmology
Wanlin Fan, Alexander C. Rokohl, Patrick Kupka, Xiaoyi Hou, Jinhua Liu, Senmao Li, Adam Kopecky, Sitong Ju, Philomena A. Wawer Matos, Yongwei Guo, Ludwig M. Heindl
Summary: In this study, the volume of customized tumor models in the periocular area was measured using three-dimensional stereophotogrammetry, and the reproducibility of these measurements was evaluated. The results showed that 3D stereophotogrammetry provides reliable measurements of periocular tumor volume, indicating its potential clinical usefulness.
OPHTHALMOLOGY AND THERAPY
(2023)
Article
Ophthalmology
Alexander C. Rokohl, Katharina Wall, Marc Trester, Philomena A. Wawer Matos, Yongwei Guo, Werner Adler, Keith R. Pine, Ludwig M. Heindl
Summary: This study compared tear film osmolarity (TFO) values and matrix metalloproteinase 9 (MMP-9) levels between anophthalmic sockets and healthy fellow eyes, and assessed the use of MMP-9 and TFO as objective biomarkers for dry anophthalmic socket syndrome (DASS). The results showed that anophthalmic sockets had higher OSDI, MMP-9, and TFO values, as well as higher conjunctival inflammation compared to healthy eyes. The findings suggest that measuring MMP-9 and TFO may be useful tools in the clinical management of DASS.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2023)
Article
Ophthalmology
Xiao-Yi Hou, Alexander C. Rokohl, Marius M. Meinke, Sen-Mao Li, Ming Lin, Ren-Bing Jia, Yong -Wei Guo, Ludwig M. Heindl
Summary: This study investigates the reliability of a modified 3D-DT and 3D-PT for assessing lower eyelid tension. The results show that the modified 3D-DT method, which involves the use of an eyelid hook, is highly reliable for evaluating LET. This novel and simple method may have potential for further investigation and routine pre-and postoperative clinical evaluation.
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Surgery
Wanlin Fan, Alexander C. Rokohl, Julia Maus, Yongwei Guo, Xiaojun Ju, Xueting Li, Philomena Wawer A. Matos, Ludwig M. Heindl
Summary: This study aimed to define the upper eyelid and upper eyelid fold region through a modified landmark strategy and validate a portable 3D imaging system for area and volume measurements in this periocular region. The results showed that the portable 3D imaging system had high reliability for upper eyelid area measurements, but had poor reliability for area and volume measurements in the upper eyelid fold region.
AESTHETIC SURGERY JOURNAL
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Yongwei Guo, Alexander C. Rokohl, Wanlin Fan, Rodothea Theodosiou, Xueting Li, Lixia Lou, Tao Gao, Ming Lin, Ke Yao, Ludwig M. Heindl
Summary: This study proposes a standardized upper eyelid region selection strategy and validates its reliability for 3D areal and volumetric measurements. The results show high reliability for areal measurement and poor reliability for volumetric measurement. This method provides quantitative data for the upper eyelids and has potential for widespread application in the future.
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2023)
Article
Surgery
Xueting Li, Alexander C. Rokohl, Wanlin Fan, Michael Simon, Xiaojun Ju, Till Rosenkranz, Philomena A. Wawer Matos, Yongwei Guo, Ludwig M. Heindl
Summary: The study quantified periocular characteristics of dermatochalasis patients using standardized 3D imaging, and compared age and sex-related changes in these features. Age was found to be a significant factor in morphological changes of certain periocular features, while gender differences were observed in specific parameters.
AESTHETIC PLASTIC SURGERY
(2023)
Review
Ophthalmology
Xueting Li, Senmao Li, Wanlin Fan, Alexander C. Rokohl, Sitong Ju, Xiaojun Ju, Yongwei Guo, Ludwig M. Heindl
Summary: Graves ophthalmopathy (GO), a complication of autoimmune thyroid disease, can significantly impact patients' quality of life. Corticosteroids have been the first-line treatment, but the use of targeted biological agents and immunosuppressive agents has shown promise. Understanding the pathogenesis and improving clinical management of GO is crucial for prognosis.
INTERNATIONAL OPHTHALMOLOGY
(2023)
Meeting Abstract
Ophthalmology
Alexander Christopher Rokohl, Katharina Wall, Marc Trester, Philomena A. Wawer Matos, Yongwei Guo, Werner Adler, Keith R. Pine, Ludwig M. Heindl
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)