Article
Biochemistry & Molecular Biology
Natalia Lomova, Natalia Dolgushina, Alisa Tokareva, Vitaly Chagovets, Natalia Starodubtseva, Ilya Kulikov, Gennady Sukhikh, Vladimir Frankevich
Summary: This study aimed to investigate the lipid profile of follicular fluid in COVID-19 patients and evaluate its impact on the outcomes of in vitro fertilization (IVF) procedures, in order to identify potential biomarkers for prognosis. Mass spectrometry was used to analyze follicular fluid samples from 237 patients, revealing significant changes in several lipids. The results suggest that lipid metabolism in follicular fluid may be associated with pregnancy occurrence and outcomes after severe COVID-19.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Kourosh Hooshmand, Jin Xu, Anja Hviid Simonsen, Asger Wretlind, Andressa de Zawadzki, Karolina Sulek, Steen Gregers Hasselbalch, Cristina Legido-Quigley
Summary: This study developed a robust lipidomics and metabolomics method based on commonly used two-phase extraction systems for exploring biochemical changes in neurodegenerative diseases. The aB&D method was found to be the most suitable for lipid extraction from human CSF samples. In combination with multiple chromatographical separation approaches, a comprehensive metabolite signature of human CSF samples was achieved.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biochemical Research Methods
Olatomiwa O. Bifarin, Samyukta Sah, David A. Gaul, Samuel G. Moore, Ruihong Chen, Murugesan Palaniappan, Jaeyeon Kim, Martin M. Matzuk, Facundo M. Fernandez
Summary: Ovarian cancer is a deadly cancer that affects the female reproductive system and is often asymptomatic until later stages. Little is known about the metabolic changes in the early stages of high-grade serous ovarian cancer. This study used a mouse model and machine learning analysis to examine the temporal changes in serum lipidome. The results showed unique alterations in cell membrane stability, proliferation, and survival during cancer development and progression, providing potential targets for early detection and prognosis of ovarian cancer.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Endocrinology & Metabolism
Ying Ding, Yihong Jiang, Mingjiang Zhu, Qinling Zhu, Yaqiong He, Yao Lu, Yuan Wang, Jia Qi, Yifan Feng, Rong Huang, Huiyong Yin, Shengxian Li, Yun Sun
Summary: This study investigated the lipidomic profiles in follicular fluid (FF) of different phenotypes of Polycystic ovary syndrome (PCOS) and identified metabolic signatures and candidate biomarkers that provide a better understanding of the pathogenesis of PCOS.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Chemistry, Analytical
Haosong Zhang, Mingdu Luo, Hongmiao Wang, Fandong Ren, Yandong Yin, Zheng-Jiang Zhu
Summary: The development of IM-MS has revolutionized the analysis of small molecules. The curation of comprehensive CCS databases plays a pivotal role in the successful application of IM-MS. AllCCS2, an enhanced version, achieved exceptional prediction accuracy and compatibility with different instrument platforms.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Sven Heiles
Summary: Metabolomics and lipidomics are driving forces in the omics era, with tandem mass spectrometry methods playing a crucial role in characterizing the structural details of metabolites and lipids. Recent advancements in alternative tandem MS methodologies aim to provide full structural characterization of these compounds. The review critically examines the principles, capabilities, drawbacks, and initial applications of these advanced tandem mass spectrometry strategies in the fields of metabolomics and lipidomics.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Stanislava Rakusanova, Oliver Fiehn, Tomas Cajka
Summary: Current metabolomics and lipidomics studies have limitations in terms of the number of examined matrices, the breadth and scope of methods, reporting the number of metabolites, and data sharing. This article discusses the concept of metabolomics and lipidomics atlases, which characterize the quantitative distribution and relationships of metabolites in biological matrices and serve as a resource for future studies. The recommended approach includes combined sample extraction, a multiplatform mass spectrometry-based method, and the use of multiple internal standards for quantification. An interactive web tool should be developed for easy access to the generated data.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
Ping Li, Yongqing Tian, Mingyi Du, Qingrong Xie, Yingying Chen, Lianlian Ma, Yudi Huang, Zhibin Yin, Hanhong Xu, Xinzhou Wu
Summary: Spatial metabolomics and lipidomics were used to investigate the metabolic expression levels and spatial distribution of rotenone-treated Plutella xylostella. The results indicated that rotenone significantly affected purine and amino acid metabolisms and destroyed the cell membrane structure. These findings contribute to a better understanding of the toxicity mechanism of rotenone on target pests.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Biochemical Research Methods
Eva-Maria Harrieder, Fleming Kretschmer, Sebastian Boecker, Michael Witting
Summary: Metabolomics and lipidomics involve the large-scale analysis of metabolites, requiring advanced analytical methods. Liquid Chromatography-Mass Spectrometry (LC-MS) is a commonly used technique that provides different selectivities in separation and high sensitivity in detection. Due to the huge chemical diversity, there is no single analysis method that can cover the entire range of metabolites or lipids, leading to the use of different separation methods. This review explores the current use of LC-MS in metabolomics and lipidomics using data from public databases, and highlights potential future trends and improvements needed.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Liesa Salzer, Michael Witting
Summary: Metabolomics and lipidomics have gained interest in the model organism C. elegans due to its fast development, easy cultivation, and existing genetic tools. Different analytical approaches such as NMR, mass spectrometry, and chromatography have been employed for metabolomic analyses. The reviewed literature also includes application fields, bioinformatic tools, and a curated collection of metabolites and lipids for a future C. elegans-specific metabolome database.
Article
Biochemistry & Molecular Biology
Eva Cifkova, Radana Brumarova, Magdalena Ovcacikova, Dana Dobesova, Katerina Micova, Ales Kvasnicka, Zuzana Vankova, Jiri Siller, Lukas Sakra, David Friedecky, Michal Holcapek
Summary: The study conducted lipidomic and metabolomic analysis on tumor and surrounding normal tissues of 23 patients with non-small cell lung cancer, identifying and quantifying 500 molecular species. Significant changes were observed in several biochemical pathways related to lung cancer, particularly involving glycerophospholipids, sphingolipids, and cholesteryl esters in lipid metabolism.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2022)
Review
Biochemistry & Molecular Biology
Simon Alesi, Drishti Ghelani, Kate Rassie, Aya Mousa
Summary: Gestational diabetes mellitus (GDM) is the fastest growing type of diabetes worldwide, with metabolomic studies highlighting deranged amino acids, carbohydrates, and lipids metabolism pathways in GDM, suggesting potential biomarkers for diagnosis and future integration into clinical practice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Vannuruswamy Garikapati, Claudia Colasante, Eveline Baumgart-Vogt, Bernhard Spengler
Summary: This study utilized a Pex11 alpha KO mouse model to investigate the molecular alterations caused by peroxisomal defects. The results showed significant changes in lipid species, metabolites, and proteins, as well as dysregulated metabolic pathways in various biological specimens. These findings provide valuable information for understanding the roles of Pex11 alpha and the underlying pathophysiological mechanisms of peroxisomal biogenesis disorders.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Neurosciences
Carolina Gonzalez-Riano, Silvia Tapia-Gonzalez, Gertrudis Perea, Candela Gonzalez-Arias, Javier DeFelipe, Coral Barbas
Summary: Brain slice preparations undergo significant changes in metabolome and lipidome, including adjustments in levels of various lipid classes and metabolites. These preparation-dependent biochemical changes related to time intervals should be considered in future studies to facilitate unbiased interpretation of experimental results.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Trine L. Toft-Bertelsen, Soren Norge Andreassen, Nina Rostgaard, Markus Harboe Olsen, Nicolas H. Norager, Tenna Capion, Marianne Juhler, Nanna Macaulay
Summary: The lipidomic signature of CSF in patients with subarachnoid hemorrhage suggests dysregulation of lipids and potential biomarkers for future treatment options.
Article
Obstetrics & Gynecology
Mariana Camargo, Emad Ibrahim, Paula Intasqui, Larissa B. Belardin, Mariana P. Antoniassi, Charles M. Lynne, Nancy L. Brackett, Ricardo P. Bertolla
Summary: Varicocele is associated with decreased semen quality and increased levels of inflammatory cytokines in seminal plasma. Varicocelectomy can lead to improvements in sperm morphology and decreased inflammatory activity in seminal plasma.
Article
Biochemistry & Molecular Biology
Evandro Silva, Jose Perez da Graca, Carla Porto, Rodolpho Martin do Prado, Estela Nunes, Francismar Correa Marcelino-Guimaraes, Mauricio Conrado Meyer, Eduardo Jorge Pilau
Summary: Research on metabolite expression in soybean plants with a resistant genotype inoculated with P. pachyrhizi revealed the potential role of flavonoids and isoflavonoids in combating Asian Soybean Rust.
Article
Biology
Dayana A. Rodrigues, Bianca L. de Sousa, Junio G. da Silva, Gustavo A. M. Pereira, Guilherme M. Bousada, Antonio A. da Silva, Antonio J. Demuner, Ederson D'M Costa, Eduardo J. Pilau, Evandro Silva, Marcelo H. dos Santos
Summary: This research focused on isolating and quantifying the major compounds from G. gardneriana seed extracts and evaluating their phytotoxic activities. The study found that guttiferone-A displayed greater herbicide activity than glyphosate, even at lower concentrations, indicating potential for development of new agrochemicals. Computational studies suggested that guttiferone-A could effectively interact with transferase enzymes, providing insights into its possible mechanism of action in plants.
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2021)
Review
Agriculture, Multidisciplinary
Evandro Silva, Joao Raul Belinato, Carla Porto, Estela Nunes, Francismar Guimaraes, Mauricio Conrado Meyer, Eduardo Jorge Pilau
Summary: Metabolomics plays a crucial role in analyzing small-molecule metabolites in organisms, particularly in agriculture and food security fields. Few studies have utilized metabolomics approach in soybean plants under biotic stress, yet metabolomics-based mass spectrometry shows potential in studying resistance and improvement mechanisms.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Plant Sciences
Thaila Fernanda Oliveira da Silva, Cristina Sayuri Yamaguchi, Susana Tavares Cotrim Ribeiro, Alexandre da Silva Avincola, Eduardo Jorge Pilau, Carla Porto, Arildo Jose Braz de Oliveira, Regina Aparecida Correia Goncalves
Summary: In this study, adventitious roots of Pfaffia glomerata were cultured in vitro under dark conditions using roller bottle and orbital gyratory systems. The quantification of beta-ecdysone by LC-MS/MS revealed the highest accumulation after 8 weeks in the roller bottle system, demonstrating the potential of adventitious root culture as an alternative strategy for beta-ecdysone production. This study highlights the practical applications of bioreactors in the continuous production of high-quality compounds.
PHYTOCHEMISTRY LETTERS
(2021)
Article
Plant Sciences
Anaclara Prasniewski, Cleidiane da Silva, Bruno Ramalho Bresolin Ayres, Evandro Aparecido da Silva, Eduardo Jorge Pilau, Bruno Dias Nani, Pedro Luiz Rosalen, Tatiane Luiza Cadorin Oldoni
Summary: The study identified the chemical composition and nutritional value of Syzygium malaccense leaves, revealing various flavonoid derivatives and phenolic compounds, as well as demonstrating anti-inflammatory activity.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Agriculture, Multidisciplinary
Lorena L. Mendes, Jodieh O. S. Varejao, Jose Antonio de Souza, Jose Walkimar de M. Carneiro, Ana K. S. M. Valdo, Felipe T. Martins, Bruno W. Ferreira, Robert W. Barreto, Toshik da Silva, Markus Kohlhoff, Eduardo J. Pilau, Eduardo V. V. Varejao
Summary: Experimental findings indicated that the presence of bulky substituents at the ortho position of a benzene ring hindered the synthesis of 2,5-DKPs, while bromine as a leaving group was more effective than chlorine in lowering the activation enthalpy for cyclization step, promoting the formation of 2,5-DKPs.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Plant Sciences
Emanuelle Magalhaes Basilio Machado da Silva Landim, Gabriel Rezende Ximenez, Evandro Silva, Matheus Ardenghi Peixoto, Eduardo Jorge Pilau, Armando Mateus Pomini, Mariza Barion Romagnolo, Lindamir Hernandez Pastorini, Silvana Maria de Oliveira
Summary: Lonchocarpus cultratus is a native tree species with potent bioactivity, including antiproliferative and allelopathic activity, for inhibiting weed and plant growth. Analysis of extracts and compounds revealed they are mainly derived from flavonoid compounds.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Jose Renato Pattaro Junior, Icaro Putinhon Caruso, Jessica Marostica de Sa, Taniara Suelen Mezalira, Diego de Souza Lima, Eduardo Jorge Pilau, David Roper, Maria Aparecida Fernandez, Flavio Augusto Vicente Seixas
Summary: The study aimed at characterizing the biophysical properties of recombinant Escherichia coli DdlB, showing that the enzyme's stability was highest at pH 5.4, with a significant presence of alpha-helix structure. The thermal denaturation process of EcDdlB was found to be irreversible, with an increase in beta-structures that may contribute to the formation of protein aggregates.
PROTEIN AND PEPTIDE LETTERS
(2022)
Article
Biochemistry & Molecular Biology
Daniela P. A. F. Braga, Amanda S. Setti, Assumpto Iaconelli Jr, Edson Borges Jr
Summary: The study aimed to investigate the impact of previous infection with SARS-CoV-2 on embryo morphokinetics and implantation. A historical cohort study was conducted in an in vitro fertilization center, including 1628 embryos from 88 patients. The results showed that embryos from patients with previous COVID-19 infection had longer duration in various developmental stages compared to the control group. However, there were no differences in clinical outcomes between the two groups.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Biochemistry & Molecular Biology
Daniela P. de Almeida Ferreira Braga, Amanda S. Setti, Patricia Guilherme, Christina Morishima, Assumpto Iaconelli, Edson Borges
Summary: The present study aimed to investigate the effect of aneuploidy on embryo morphokinetics events using a time-lapse imaging (TLI) system incubator. A retrospective cohort study was conducted in a private university-affiliated in vitro fertilization center between March 2019 and December 2020. Our findings suggest that TLI monitoring may be an adjunct approach to select embryos for preimplantation genetic testing (PGT), but further investigation is still needed.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Andrology
Daniela Paes Almeida Ferreira Braga, Amanda Setti, Christina Morishima, Rodrigo R. Provenza, Assumpto Iaconelli Jr, Edson Borges Jr
Summary: This study aimed to investigate the relationship between sperm DNA fragmentation and intracytoplasmic sperm injection (ICSI) outcomes, and whether this relationship is influenced by the presence of oocyte dimorphisms. The results showed that ICSI cycles with lower sperm DNA fragmentation had higher rates of fertilization, high-quality embryos, implantation, and pregnancy. The presence of oocyte dimorphisms significantly affected the outcomes. The lowest rates of fertilization and high-quality embryos were observed when high sperm DNA fragmentation was associated with certain oocyte defects, such as dark cytoplasm, vacuoles, resistant membrane, and non-resistant membrane.
Review
Chemistry, Medicinal
Mafalda Bacalhau, Mariana Camargo, Grace A. V. Magalhaes-Ghiotto, Sybelle Drumond, Carlos Henrique M. Castelletti, Miqueias Lopes-Pacheco
Summary: Cystic fibrosis is a potentially fatal monogenic disease that has been significantly improved by the introduction of CFTR modulator drugs, particularly the triple combination therapy of elexacaftor, tezacaftor, and ivacaftor (ETI), which has shown safety and efficacy in reducing various disease symptoms. However, close monitoring by a healthcare team is necessary due to possible adverse effects associated with ETI therapy. This review aims to discuss the therapeutic benefits and adverse effects of ETI therapy for cystic fibrosis patients.
Article
Food Science & Technology
Adrieli Rodrigues da Costa Nunes, Claudete Aparecida Mangolin, Arildo Jose Braz de Oliveira, Regina Aparecida Correia Goncalves, Alexandre da Silva Avincola, Rafaela Takako Ribeiro de Almeida, Eduardo Jorge Pilau, Maria de Fatima Pires da Silva Machado
Summary: The study found that the cladode extract from Cereus peruvianus grown in the southern region of Brazil contains abundant phenolic compounds and antioxidant activity, including flavonoids that have not been reported before. These phenolic compounds linked to antioxidant activities support the use of this species as a source of natural antioxidants in human food.
CURRENT RESEARCH IN FOOD SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Alex Graca Contato, Caroline Aparecida Vaz de Araujo, Daniele Maria Zanzarin, Guilherme Mauro Aranha, Priscila Ayumi Sybuia, Eduardo Jorge Pilau, Rafael Castoldi, Rosane Marina Peralta, Cristina Giatti Marques de Souza
Summary: This study aimed to evaluate the antimicrobial capacity and to separate and characterize bioactives from the mycelium of medicinal mushrooms Pleurotus albidus and Phellinus linteus. Results showed that P. albidus extracts had better antimicrobial activity against Bacillus strains, while Ph. linteus extracts were more effective against Staphylococcus aureus and Pseudomonas aeruginosa. Aqueous extraction was found to be the best method for obtaining bioactive compounds of P. albidus, while 30% hydroalcoholic extraction performed best for obtaining Ph. linteus. The identification of the main chemical compounds extracted from fungal biomasses was done through mass spectrometry analyses.
INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS
(2022)
