Article
Multidisciplinary Sciences
R. Sainte-Rose, C. Petit, L. Dijols, C. Frapsauce, F. Guerif
Summary: The study found that blastocyst development rate is significantly reduced in advanced maternal age patients, with blastocysts showing growth retardation on day 5. While cell organization is unaffected by age, blastocysts with 'good' morphology in AMA patients have decreased implantation probability compared to 'average' morphology blastocysts in younger women. Although extended embryo culture is not harmful for AMA patients, it cannot compensate for the reduced implantation and live birth rates associated with maternal age.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Zhuoning Zou, Chuanxin Zhang, Qiuyan Wang, Zhenzhen Hou, Zhuqing Xiong, Feng Kong, Qiujun Wang, Jinzhu Song, Boyang Liu, Bofeng Liu, Lijuan Wang, Fangnong Lai, Qiang Fan, Wenrong Tao, Shuai Zhao, Xiaonan Ma, Miao Li, Keliang Wu, Han Zhao, Zi-Jiang Chen, Wei Xie
Summary: This study utilized Ribo-lite and mRNA sequencing to analyze the transcriptome and translatome of human oocytes and early embryos. The results showed widespread differential translation during the oocyte-to-embryo transition (OET) and zygotic genome activation (ZGA), functioning in epigenetic reprogramming, transposon defense, and small RNA biogenesis. Additionally, TPRX1/2/L were identified as critical regulators of ZGA.
Article
Multidisciplinary Sciences
Shizhe Yu, Haoren Wang, Lingpeng Yang, Yingxue Yan, Qiang Cai, Duo Ma, Long Jiang, Zehai Gao, Zhiyong Yu, Zongping Xia
Summary: The comprehensive study of the spatial-cellular anatomy of the human liver is crucial for understanding the cellular origins of liver disease. In this study, spatial transcriptomics were conducted on normal human liver tissue sections, providing detailed transcriptional information on liver zonation. A total of 6581 high-quality spots from normal livers were analyzed, mainly consisting of hepatocytes, which were classified into four sub-groups. These data serve as a reliable reference for studying the spatial heterogeneity of liver lobules.
Article
Biochemical Research Methods
Romain Bulteau, Mirko Francesconi
Summary: RAPToR is a computational method based on transcriptomic data that precisely estimates the real age of a specimen. It can remove age as a confounding variable and recover the signal of interest in differential expression analysis.
Article
Multidisciplinary Sciences
Jian Lu, Raheel Ahmad, Thomas Nguyen, Jeffrey Cifello, Humza Hemani, Jiangyuan Li, Jinguo Chen, Siyi Li, Jing Wang, Achouak Achour, Joseph Chen, Meagan Colie, Ana Lustig, Christopher Dunn, Linda Zukley, Chee W. Chia, Irina Burd, Jun Zhu, Luigi Ferrucci, Nan-Ping Weng
Summary: The decline of CD8(+) T cell functions with aging is a significant health issue, and this study uses single-cell RNA sequencing and machine learning to understand the mechanisms and predict the age of individual cells. This research has important implications for predicting functional outcomes in vaccination or infection.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Jae Kyun Park, So-Yeon Ahn, Su Hee Seok, Eun Mi Chang, Ji Won Kim, In Pyung Kwak, Woo Sik Lee
Summary: This study investigated the association between post-warming extended culture duration and pregnancy outcomes in patients of advanced maternal age (AMA) undergoing vitrified-warmed blastocyst transfer (SVBT). The results showed that there were no significant differences in the live birth rates (LBR) between AMA patients and younger patients based on blastocyst morphology and post-warming extended culture duration. This finding suggests that post-warming extended culture of blastocysts is not harmful to AMA patients and could be useful in clinical counseling for fertility treatments.
JOURNAL OF KOREAN MEDICAL SCIENCE
(2022)
Article
Cell Biology
Christopher A. Wolff, Miguel A. Gutierrez-Monreal, Lingsong Meng, Xiping Zhang, Lauren G. Douma, Hannah M. Costello, Collin M. Douglas, Elnaz Ebrahimi, Ann Pham, Aline C. Oliveira, Chunhua Fu, Amy Nguyen, Bryan R. Alava, Stuart J. Hesketh, Andrew R. Morris, Mehari M. Endale, G. Ryan Crislip, Kit-yan Cheng, Elizabeth A. Schroder, Brian P. Delisle, Andrew J. Bryant, Michelle L. Gumz, Zhiguang Huo, Andrew C. Liu, Karyn A. Esser
Summary: Cellular circadian clocks regulate daily transcriptional program and are important for maintaining homeostasis and resilience. Age-related changes in circadian functions have been observed. This study characterized age-dependent changes in the circadian transcriptome in multiple tissues, revealing tissue-specific alterations in clock output and decreased number of rhythmically expressed genes (REGs) with aging. Analysis of differentially expressed genes (DEGs) at different times of day identified distinct gene expression patterns, with increased variability in aged tissues. This study expands our understanding of aging and highlights the impact of aging on circadian clock function and temporal gene expression changes.
Article
Multidisciplinary Sciences
Juquan Song, Steven G. Widen, Steven E. Wolf, Amina El Ayadi
Summary: This study demonstrates the characteristic profile of gene expression in skeletal muscle in young and adult burned mice. Prominent age effects were revealed in transcriptional levels with increased alterations of genes, miRNAs, pathways, and interactions.
SCIENTIFIC REPORTS
(2022)
Article
Oncology
Lingyue Hua, Wei Chen, Yan Meng, Meng Qin, Zhiqiang Yan, Rui Yang, Qiang Liu, Yuan Wei, Yangyu Zhao, Liying Yan, Jie Qiao
Summary: This study revealed that advanced maternal age (AMA) induces aging-like changes in DNA methylome and gene expression in both parents and offspring. The findings suggest a potential intergenerational inheritance of AMA-related DNA methylome and transcriptome, providing new insights into the health problems in AMA offspring.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Reproductive Biology
Agata Zmijewska, Wioleta Czelejewska, Ewa M. Drzewiecka, Anita Franczak
Summary: The pituitary gland plays a crucial role in female reproduction. However, little is known about the changes in the transcriptome of the porcine pituitary during reproductive status. This study aimed to analyze and compare the transcriptome profile of the pituitary gland in pregnant pigs and pigs during the estrous cycle. The analysis revealed 482 differentially expressed genes, with 68 up-regulated and 414 down-regulated in pregnant pigs. These findings provide insights into the molecular mechanisms involved in maternal recognition of pregnancy in pigs.
Article
Obstetrics & Gynecology
P. Ntostis, D. Iles, G. Kokkali, T. Vaxevanoglou, E. Kanavakis, A. Pantou, J. Huntriss, K. Pantos, H. M. Picton
Summary: The study found a decrease in mitochondrial-related transcripts in MII oocytes with advanced age, indicating reduced energy potential with ageing.
HUMAN REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Stephanie M. Zimmerman, Robin Fropf, Bridget R. Kulasekara, Maddy Griswold, Oliver Appelbe, Arya Bahrami, Rich Boykin, Derek L. Buhr, Kit Fuhrman, Margaret L. Hoang, Quoc Huynh, Lesley Isgur, Andrew Klock, Alecksandr Kutchma, Alexa E. Lasley, Yan Liang, Jill McKay-Fleisch, Jeffrey S. Nelson, Karen Nguyen, Erin Piazza, Aric Rininger, Daniel R. Zollinger, Michael Rhodes, Joseph M. Beechem
Summary: Emerging spatial profiling technology allows high-plex molecular profiling in biological tissues while preserving the spatial and morphological context of gene expression. By expanding the chemistry for the Digital Spatial Profiling platform, researchers have successfully quantified whole transcriptomes in human and mouse tissues, enabling applications in cancer research, molecular pathology, and developmental biology.
Article
Biotechnology & Applied Microbiology
Emilie Derisoud, Luc Jouneau, Cedric Dubois, Catherine Archilla, Yan Jaszczyszyn, Rachel Legendre, Nathalie Daniel, Nathalie Peynot, Michele Dahirel, Juliette Auclair-Ronzaud, Laurence Wimel, Veronique Duranthon, Pascale Chavatte-Palmer
Summary: This study found that maternal age affects gene expression in equine blastocysts. As mares age, gene expression related to mitochondria and protein metabolism is disrupted, leading to impaired embryo development and decreased fertility.
Review
Biochemistry & Molecular Biology
Laszlo Tora, Stephane D. Vincent
Summary: In somatic cells, transcription initiation by Pol II depends on TFIID/TBP binding to core promoters, while in growing oocytes, TBPL2 replaces TBP for active transcription. Maternal mRNAs stored in oocytes are inherited by zygotes after fertilization, serving as the sole source for protein translation until zygotic genome activation. Reshaping of the maternal transcriptome involves readenylation, translation, and degradation of stored transcripts, leading to clearance after zygotic genome activation. Recent studies have shed light on the regulation of maternal mRNA dynamics in mice.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Nutrition & Dietetics
Chang Cao, Miguel A. Prado, Liang Sun, Shira Rockowitz, Piotr Sliz, Joao A. Paulo, Daniel Finley, Mark D. Fleming
Summary: Maternal iron deficiency has limited impact on placental gene expression in mice during mid-gestation, mainly mediated by IRE coordination of iron gene expression. Interestingly, sexually dimorphic effects of maternal iron deficiency on placental gene expression were observed, with more alterations in male mouse placentas.
JOURNAL OF NUTRITION
(2021)
Article
Multidisciplinary Sciences
Michelle M. Denomme, Jason C. Parks, Blair R. McCallie, Nathan McCubbin, William B. Schoolcraft, Mandy G. Katz-Jaffe
Article
Genetics & Heredity
Blair R. McCallie, Mary Haywood, Michelle M. Denomme, Rachel Makloski, Jason C. Parks, Darren K. Griffin, William B. Schoolcraft, Mandy G. Katz-Jaffe
Summary: The study identified significant DNA variants associated with diminished ovarian reserve (DOR) in young women, particularly impacting the Glucocorticoid receptor signaling pathway. Further analysis revealed a cut-off at 31 years for distinguishing young DOR patients. Embryonic global methylome sequencing showed minimal methylation alterations in the DOR group, while RNA sequencing indicated no significant transcription changes between DOR blastocysts and controls.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Developmental Biology
Blair R. McCallie, Jason C. Parks, Darren K. Griffin, William B. Schoolcraft, Mandy G. Katz-Jaffe
MOLECULAR HUMAN REPRODUCTION
(2017)