Article
Plant Sciences
Koichi Yoshioka, Hoon Kim, Fachuang Lu, Nette De Ridder, Ruben Vanholme, Shinya Kajita, Wout Boerjan, John Ralph
Summary: A previously overlooked benzofuran moiety was discovered in lignin, revealing its potential mechanistic role in lignification.
Review
Biochemistry & Molecular Biology
Christopher J. Robinson, Jonathan Tellechea-Luzardo, Pablo Carbonell, Adrian J. Jervis, Cunyu Yan, Katherine A. Hollywood, Mark S. Dunstan, Andrew Currin, Eriko Takano, Nigel S. Scrutton
Summary: Metabolic engineering technologies have been successfully employed for the engineering and optimization of industrial host strains over the past three decades. Design-Build-Test-Learn pipelines are being established to rapidly deliver diverse chemical targets through iterative optimization of microbial production strains. Biofoundries are using in silico tools for genetic design and combinatorial design of experiments to optimize selection within the potential design space based on multi-criteria objectives.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Plant Sciences
Shan Jiang, Xu Tian, Xiaolong Huang, Jiankang Xin, Huiqing Yan
Summary: The CAD multigene family in P. patens consists of four genes. PpCADs have the potential to synthesize monolignols by catalyzing NADPH-dependent reduction. While PpCAD1 did not produce conventional monomers of lignin, it promoted phenylpropanoids to modify plant architecture and development, potentially enhancing resistance against pathogens.
Article
Plant Sciences
Youngsung Joo, Hoon Kim, Moonyoung Kang, Gisuk Lee, Sungjun Choung, Harleen Kaur, Shinyoung Oh, Jun Weon Choi, John Ralph, Ian T. Baldwin, Sang-Gyu Kim
Summary: Plants have developed tissue-specific defense mechanisms against different herbivores, such as upregulating lignin-associated genes in the inner parenchymal cells of stems after attack by stem-boring herbivores. This induced lignification in response to stem-boring herbivores is modulated by jasmonate and ethylene signaling pathways.
Article
Horticulture
Dan Wang, Qiyang Chen, Weiwei Chen, Qigao Guo, Yan Xia, Di Wu, Danlong Jing, Guolu Liang
Summary: The study found that MT treatment can reduce weight loss and firmness decline in loquat fruit during cold storage, while maintaining the sweetness of the fruit. MT also increases the content of phenolic compounds and organic acids in the fruit, as well as enhancing antioxidant capacity. Additionally, MT treatment regulates the activities of lignin biosynthesis-related enzymes.
SCIENTIA HORTICULTURAE
(2021)
Article
Forestry
Guosong Dong, Qunqun Guo, Wenjun Deng, Yu Zhang, Hongzheng Tai, Chao Wang, Guicai Du, Ronggui Li
Summary: This study selected the cinnamyl alcohol dehydrogenase gene (BxCAD-1) from the pine wood nematode (PWN) to investigate the pathogenic mechanism of pine wilt disease (PWD). The BxCAD-1 gene was cloned and expressed in Escherichia coli, and its activity was characterized under different conditions. By using RNA interference, the functions of BxCAD-1 in the nematode were down-regulated, resulting in decreased movement, feeding, reproduction, and lifespan. The study also showed that BxCAD-1 was expressed in the digestive tract of male and female adults. These findings provide a promising target for PWD control.
Article
Plant Sciences
Nan Chao, Shuai Huang, Xiaoru Kang, Keermula Yidilisi, Mingjie Dai, Li Liu
Summary: In this study, five MaCADs were cloned and characterized in Morus alba. The results showed that MaCAD3/4 were bona fide CADs involved in lignin biosynthesis. Down-regulation of MaCAD3/4 resulted in a significant decrease in lignin content in both stems and leaves. MaCADs exhibited different expression patterns in response to various stresses, indicating their possible diverse roles. These findings provide an important foundation for the genetic modification of the monolignol pathway in mulberry.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Plant Sciences
Tengxun Zhang, Fei Bao, Aiqin Ding, Yongjuan Yang, Tangren Cheng, Jia Wang, Qixiang Zhang
Summary: Floral scent is an important trait of Prunus mume and shows significant differences in composition among cultivars. Cinnamyl alcohol is a key floral volatile compound and PmCAD1 plays a crucial role in its synthesis. This study provides a theoretical foundation for understanding the molecular biosynthesis mechanism of floral volatiles in P. mume.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Xing Huang, Bochao Xu, Shibei Tan, Yanlei Huang, Jingen Xi, Xu Qin, Tao Chen, Helong Chen, Xiaohan Yang, Kexian Yi
Summary: This study identified the evolutionary and expression characteristics of the CAD gene family in agave, providing potential targets for improving lignin traits in the future. The transcriptome dataset of A. angustifolia presented here will facilitate further research on lignin biosynthesis in agave.
Article
Genetics & Heredity
Lei Hu, Xuan Zhang, Huihui Ni, Fangyu Yuan, Shuiming Zhang
Summary: This study identified and studied the expression correlation of the pomegranate CAD gene family. The family has 25 members distributed on seven chromosomes. The family was divided into four groups, with one involved in lignin synthesis and another in stress resistance. Gene expression analysis revealed multiple functions of the PgCAD gene family.
Article
Biochemistry & Molecular Biology
Yankai Li, Yakun Pei, Wenwen Yu, Wenjun Wu, Ding Li, Zhaonong Hu
Summary: By combining bioinformatics analysis and in vitro enzyme assays, this study elucidated the structure and function of the CAD gene family in Phryma leptostachya, identifying the CAD isoform with the highest catalytic activity and providing valuable insights into furofuran lignans biosynthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Kamolwan Khianchaikhan, Suvichark Aroonluk, Supachai Vuttipongchaikij, Chatchawan Jantasuriyarat
Summary: In this study, the oil palm homeodomain leucine zipper (EgHD-ZIP) family was identified and found to play a crucial role in embryogenesis. The expression analysis revealed that EgHD-ZIP IV genes were up-regulated at the late stages of somatic embryogenesis, while the BBM gene was up-regulated at the early stage. Furthermore, the direct binding between members of the EgHD-ZIP IV subfamily was confirmed. These findings are important for addressing the challenges in oil palm tissue culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agronomy
Yuanyuan Hou, Liangyi Zhao, Bing Xie, Shunqing Hu, Yonghua Zheng, Peng Jin
Summary: CaCl2 treatment alleviates chilling-induced lignification in loquat fruit, probably via the synergistic role of EjCaM7 and EjCAMTA3 in the modulation of EjPOD64 and EjLAC12-like gene expression, leading to the repression of lignin polymerization.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Plant Sciences
Yu Yan, Peng Wang, Yi Lu, Yujing Bai, Yunxie Wei, Guoyin Liu, Haitao Shi
Summary: The molecular mechanism underlying drought stress resistance in cassava involves MeRAV5 modulating H2O2 and lignin levels. Silencing of MeCAD15 or MeRAV5, either separately or together, results in lower drought stress resistance in cassava, which can be alleviated by exogenous lignin treatment.
Article
Biotechnology & Applied Microbiology
Chong Yu Lok Yusuf, Nuraini Sabri Nabilah, Nur Atiqah Amiza Mohd Taufik, Idris Abu Seman, Mohd Puad Abdullah
Summary: Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme involved in lignin biosynthesis in plants. This study identified and analyzed seven CAD genes in oil palm, with a focus on two genes, EgCAD1 and EgCAD2. The results showed that EgCAD1 and EgCAD2 are involved in lignin biosynthesis during normal development and response to stress in oil palm, and their 5' flanking regions contain important regulatory elements.
Article
Agriculture, Multidisciplinary
Xiangmei Cao, Kaili Xie, Wenyi Duan, Yunqi Zhu, Mingchun Liu, Kunsong Chen, Harry Klee, Bo Zhang
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Chemistry, Analytical
Xiaoyong Zhao, Cheng Guo, Yu Huang, Lili Huang, Ge Ma, Yaqin Liu, Quan He, Huiwen Wang, Kunsong Chen, Yuanjiang Pan
ANALYTICAL CHEMISTRY
(2019)
Article
Plant Sciences
Meng Xu, Shao-jia Li, Xiao-fen Liu, Xue-ren Yin, Donald Grierson, Kun-song Chen
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Plant Sciences
Jing Zhang, Xue-Ren Yin, Heng Li, Meng Xu, Meng-Xue Zhang, Shao-Jia Li, Xiao-Fen Liu, Yan-Na Shi, Donald Grierson, Kun-Song Chen
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Agriculture, Multidisciplinary
Qiang Lyu, Xin Wen, Yilong Liu, Chongde Sun, Kunsong Chen, Cheng-Chih Hsu, Xian Li
Summary: The chemical structural analysis identified various phenolic compounds in red and white bayberries, including 18 flavonoid glycosides and 13 proanthocyanidins. Biqi showed an increase in flavonoid glycoside content and a decrease in PACs during fruit maturation, while Shuijing exhibited a decrease in PAC levels without accumulating flavonoid glycosides during fruit development.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Menghua Lin, Jiahui Chen, Di Wu, Kunsong Chen
Summary: Mechanical damage to fruit causes flavor changes during post-harvest supply chains. The study identified hexanal and ethyl acetate as potential volatile biomarkers for detecting damaged apples. Transcription factors may contribute to the accumulation of hexanal and ethyl acetate by regulating the expression of LOX pathway-related genes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Yuting Ma, Song Wang, Ziyang Zhang, Xiangmei Cao, Bo Zhang, Di Wu, Kunsong Chen, Wen-Jun Wang, Pingwei Liu
Summary: COF-based hollow nanoparticles with good water dispersibility and thermoresponsive controlled release properties have potential in food preservation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Plant Sciences
Meng Xu, Hui Li, Zhen-Ning Liu, Xiao-Hua Wang, Ping Xu, Sheng-Jie Dai, Xue Cao, Xiao-Yu Cui
Summary: The study showed that GmCIPK2 positively regulates drought tolerance and ABA signaling in plants, enhancing plant adaptation to drought stress by affecting stomatal closure and the expression of drought-responsive genes. Additionally, it was demonstrated that GmCIPK2 physically interacts with a positive regulator of drought stress, GmCBL1, on the plasma membrane.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
